• Journal of Plant Biotechnology
• /
• v.43 no.2
• /
• pp.213-222
• /
• 2016

This study aims to establish a system for the rapid discrimination of Zoysia species using metabolite fingerprinting of FT-IR spectroscopy combined with multivariate analysis. Whole cell extracts from leaves of 19 identified Zoysia japonica, 6 identified Zoysia sinica, and 38 different unidentified Zoysia species were subjected to Fourier transform infrared spectroscopy (FT-IR). PCA (principle component analysis) and PLS-DA (partial least square discriminant analysis) from FT-IR spectral data successfully divided the 25 identified turf grasses into two groups, representing good agreement with species identification using molecular markers. PC (principal component) loading values show that the $1,100{\sim}950cm^{-1}$ region of the FT-IR spectra are important for the discrimination of Zoysia species. A dendrogram based on hierarchical clustering analysis (HCA) from the PCA and PLS-DA data of turf grasses showed that turf grass samples were divided into Zoysia japonica and Zoysia sinica in a species-dependent manner. PCA and PLS-DA from FT-IR spectral data of Zoysia species identified and unidentified by molecular markers successfully divided the 49 turf grasses into Z. japonica and Z. sinica. In particular, PLS-DA and the HCA dendrogram could mostly discriminate the 47 Z. japonica grasses into two groups depending on their origins (mountainous areas and island area). Considering these results, we suggest that FT-IR fingerprinting combined with multivariate analysis could be applied to discriminate between Zoysia species as well as their geographical origins of various Zoysia species.

• Weed & Turfgrass Science
• /
• v.4 no.4
• /
• pp.405-412
• /
• 2015

Forty schools having natural turf playgrounds were investigated by on-spot investigation and oral interviews with relevant school officials to find out basic information on turfgrass management practices of school playgrounds in Gyeonggi province. Average area of playground was $3,890m^2$ per school and $12m^2$ per student. Ninety five percent of turf playgrounds were managed by school staff and ninety percent of schools spent less than 5 million won per year for turf management. The difficulties in turfgrass management were considered as a major challenge for the schools, followed by turfgrass management cost. Among the management practices, school officials pointed out weed management as the most difficult work, followed by irrigation. The average number of fertilization and mowing was 2 and 6 times per year, respectively. About the half of playgrounds were irrigated only when there was wilting symptom. Zoysiagrass was the most popular choice for the school playgrounds and only three school playgrounds were established with pop-up irrigation system. Fourteen school playgrounds had good turf quality but the rest of school playgrounds had inadequate turf quality requiring minor or full renovation.

• Asian Journal of Turfgrass Science
• /
• v.26 no.2
• /
• pp.89-95
• /
• 2012

Korean lawn grass, which belongs to Zoysia genus in Gramineae family, is one of the major turfgrass which is widely adapted to its native niches in the temperate region of the north east Asia through tropical regions including Philippine, Thailand and Australia. The Turfgrass Information Center' database provides 5,340 descriptive records including 638 referred papers and 1,370 technical reports concerning 'Zoysia'. The database focused on researches on golf courses and lawn care industries. The researches provide informations on seed technology and use of plant protectant for pest management in turf management industries. The purpose of this study is to analyze and classify the research contents of zoysiagrasses which have been published in Korean journals. The total number of research papers published in Korean journals were 274 including 102, 38, and 134 in the 'Botanical', 'Environmental' and 'Cultural' researches, respectively. Publication in foreign journals by Korean investigators were not counted in this research. Research fields concerning golf course maintenance and plant protectant are believed to be necessary when compared to international trends. Moreover, advanced research efforts for the development of new cultivars with various environmental and disease resistance should be accomplished.

• Weed & Turfgrass Science
• /
• v.6 no.1
• /
• pp.40-54
• /
• 2017

The mound grave of Korean traditional funeral culture is a unique habitat which is a Zoysiagrass lawn being sustainable in proper management. We phytosociologically described an unusual Zoysiagrass vegetation with Gueldenstaedtia verna by the Zürich-Montpellier School's method and analyzed eco-floristic characteristics. A new association, Gueldenstaedtio-Zoysietum japonicae ass. nov. hoc loco, was identified and subdivided into festucetosum ovinae, typicum, and trifolietosum repensae. The subassociations were distinguished by the difference in species composition resulting from site accessibility and lawn management method. The association was assigned as not only a regional but also a locally-limited vegetation type, which distributes on a particular microhabitat with strong continentality in the Daegu regional subdistrict of Bioclimatological division. The Zoysiagrass vegetation of Korea was considered as a continental type apparently different from the oceanic type of Zoysion japonicae (Miscanthetea sinensis, Caricetalia nervatae). Mound graves in Korea should be acknowledged as a meaningful habitat for the in-situ conservation of biodiversity and phytocoenosen, despite being a secondary grassland of Gueldenstaedtio-Zoysietum japonicae.

• Asian Journal of Turfgrass Science
• /
• v.26 no.2
• /
• pp.129-134
• /
• 2012

Research was focused on the improvement of poor drainage problems on golf course fairway which had not been performed soil test or properly amended during the course construction. The analysis of the drainage problem basically was caused by a deterioration of soil physical properties by the top layer compaction. The soil hardness reached about 3,000 Kpa around 5~6 cm of soil profile. The slow infiltration speed to subsoil by the compaction was caused directly a poor drainage capacity. However, the properly amended sand soil showed an apparent value of 1,500 Kpa through the subsoil. The water content test showed a similar result that higher rate of 20~30% and ideal rate of 8~12% at poor drainage area and successfully amended area, respectively. However, an imported topsoil media which had higher content of silt and clay from a trans-planted sod had made a heterogeneous soil profile and that caused a poor drain capacity by a low infiltration rate. Those drainage problems triggered to buildup a reduced soil layer by poor soil gas exchange. The soil environment of deoxidation enhanced anaerobic microbial population and induced methane gas build-up to 55 ppm, and that resulted an adverse effect on turf growth by root growth retardation, consequently.

• Weed & Turfgrass Science
• /
• v.4 no.4
• /
• pp.368-375
• /
• 2015

Earthworm cast occurred in surface of turfgrass in golf courses which affect turfgrass maintenance and golf play. In this study several survey of seasonal fluctuation of earthworm cast in different golf courses (Anseong, Gapyeong, Gunpo and Yongin in Gyeonggi province, and Geumjeong in Busan) was done. A number of earthworm, soil temperature and moisture of detection site of earthworm cast in each golf course were also done. Cast occurred in different golf courses are found mostly from April to November on turf surface and the cast number varied in different month as well as in golf courses. In the same golf courses, a number of detected cast was difference from individual sites. A number of cast and earthworm was correlated. Also, in higher soil moisture showed the higher cast density in different golf courses. Soil temperature and moisture of detecting cast ranging from $2.1^{\circ}C$ to $33.1^{\circ}C$ and 4.9 to 44.1%, respectively. In case of cast, soil temperature level lies in between $10-15^{\circ}C$ where the highest soil moisture was 25%.

• Weed & Turfgrass Science
• /
• v.2 no.1
• /
• pp.55-61
• /
• 2013

This study was carried out to investigate the morphological and soil characteristics of Zoysia spp. native to South Korea. Samples [41 ecotypes of Zoysia japonica, 28 ecotypes of natural hybride between Z. japonica and Z. sinica (Junggi), 22 ecotypes of Z. sinica, and 8 ecotypes of Z. matrella] were collected in seashores, levee and summit of the rock in southern Korea. Variations in leaf width, plant height, leaf angle, length of leaf sheath, leaf trichome, stolon length, and seed shape were measured at collection sites and experimental plots. Among the entries, most of the measurements did not show any significant differences between natural and experimental plot except for leaf angle of Z. sinica and the number of seeds per spike of Z. matrella, which might be caused by different environmental conditions. Soil pH was 6.0 at the most of the collection sites. $Mg^+$ was 0.06-0.02 $cmol{_c}^+kg^{-1}$, $Mg^{2+}$ was 0.09-0.03 $cmol{_c}^+kg^{-1}$, and $K^+$ was 0.02-0.007 $cmol{_c}^+kg^{-1}$ at most zoysiagrasses growing soils.

• Asian Journal of Turfgrass Science
• /
• v.24 no.2
• /
• pp.125-130
• /
• 2010

Dollar spot and brown patch disease are the most economically important disease for highly maintained turfgrass area such as golf courses. Previous researches indicated that various natural fertilizers are good nitrogen (N) sources and they may lead to turf disease suppression because disease suppression by natural N sources may be due to microbial activity increased. Increased microbial activities in soil reduce the activity of plant pathogen. The objective of this study was to evaluate efficacy of various N sources to suppress dollar spot and brown patch on creeping bentgrass. The seven N sources and fungicide combinations were investigated for turf disease suppression. $Emerald^{TM}$ and $ProStar^{TM}$ were applied for the applications of fungicide combination. No differences were found on turf quality among N source treatments. However, there was significant difference on turf quality between fungicide combination and fungicide combination plus urea. Overall, N source had no significant effects on suppression of dollar spot and brown patch. Although there were a few differences among N source treatments, fungicide treatments were needed for turf disease suppression for highly maintained turfgrass area such as golf courses.

• Weed & Turfgrass Science
• /
• v.4 no.3
• /
• pp.262-267
• /
• 2015

In order to establish the efficient sod production and soil management, there is a need to perform research on the growing condition of zoysiagrass on soil environments. With an attempt to identify the growth of zoysiagrass and the chemical characteristics of soil according to different growing seasons, this study was carried out in separate areas where zoysiagrass has been grown for 1 year, 10 years, 20 years, and 30 years. As the growing season became longer, bulk density of the soil was increased, porosity and gaseous phase were decreased. The level of pH was highest in the area where zoysiagrass has been produced for 30 years, whereas total nitrogen and organic matters were found to be the greatest in where zoysiagrass has been produced for 1 year. Accordingly, the chemical properties of soil were deteriorated more in the area with continuous cropping than in the area with 1 year of cropping. As the time period of producing zoysiagrass became longer, growth of shoot and root were decreased. In this study, it is required to produce zoysiagrass through soil improvement in areas that have been used for production for over 10 years.

• Weed & Turfgrass Science
• /
• v.6 no.3
• /
• pp.249-261
• /
• 2017

To prevent large patch disease, caused by Rhizoctonia solani AG-2-2, in zoysiagrass a fungicide, Tebuconazole and three microbial agents Streptomyces sp. Burkholderia sp. and Streptomyces sp. S8 were applied in commercial turfgrass cultivation field in Sanchung, Gyeongnam, Korea. All treatments showed 50% reduced the pathogen population in thatch layer throughout the yearly cultivation period. Not only reduced the pathogen population, Tebuconazole, Streptomyces sp. Burkholderia sp. and Streptomyces sp. S8 treatment also enhanced turfgrass growth, chlorophyll and proline content. Malondialdehyde contents in each treatment was reduced from 6.2~28.9% when compared with the control. Taken together, reduction of pathogen population in soil lowered the disease incidence or severity, and allowed the turfgrass developed as normal condition. The results suggested that the selected microbial agents may use as biological control and growth promotion agents for the Zoysia turfgrass.