• Title/Summary/Keyword: 자외선-B

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OZONE MEASUREMENTS IN THE STRATOSPHERE FROM KSR420S-1 AND -2 (과학 1, 2호 로켓 실험을 통한 성층권 오존량 측정)

  • Lee, K. Y.;Lee, D. H.;Kim, J.;Park, C. J.;Cho, H. K.
    • Journal of Astronomy and Space Sciences
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    • v.11 no.1
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    • pp.53-70
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    • 1994
  • The Korean sounding rockets(KSR420S-1, -2) equipped with ozone detectors have b3en launched at An-heung, Chungchungnam-do, on June 4 and September 1, 1993, respectively. The ozone detector is used to measure the attenuation of solar UV radiation for various frequency bands in the stratosphere, to obtain vertical profiles of the ozone number density in the stratosphere. They confirm that the maximum ozone densities occur near 25 km, which is quite consistent with the mean value in the mid-latitude region. Our results from KSR420S-1 and -2 are compared with the other observation data from the Dobson spectrophotometer at Yonsei Univ., the LIDAR at Kyunghee Univ., the SBUV from Nimbus satellite, and the TOVS from NOAA satellite, which were performed simultaneously with the sounding rocket experiments.

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Effect of Iron(II)-ascorbate Complex on Protein and DNA of Phages (파아지 단백질 및 DNA에 대한 2가철-아스코르빈산착체의 영향)

  • Lho, Il-Hwan;Murata, Akira
    • Korean Journal of Food Science and Technology
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    • v.25 no.1
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    • pp.46-51
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    • 1993
  • The inactivating effect of iron(II)-ascorbate complex (Fe-Asc) on various phages was previously reported. This paper describes the molecular target in the phage virion attacked by Fe-Asc. The effect of Fe-Asc on protein was investigated with bovine serum albumin and the structural protein of phage J1. There were no differences in the SDS-polyacrylamide gel electrophoresis (patterns of these two proteins when either they were treated) with Fe-Asc or not. Also, there were no changes in the amino acid composition and ultraviolet spectrum of the proteins. The effects of Fe-Asc on DNA was investigated with pUC18 DNA, M13mpB DNA and ${\lambda}$ DNA as well as DNA from phage J1. Fe-Asc caused initially nicking of the subsequently form of pUC18 DNA to yield the open circular form and then subsequently the linear form. Strand breaks were also confirmed with M13mp8 DNA and ${\lambda}$ DNA as well as J1 DNA. The results indicate that the strand breaks in phage DNA could be responsible for the inactivation of phages by Fe-Asc.

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Genetic Analysis of Recombinants by Interspecific Protoplast Fusion of Coryneform Bacteria and Their L-glutamate & L-glutamine Production (Corynebacterium 세균의 이종간 원형질체 융합에 의한 재조합주의 유전학적 분석과 L-glutamate와 L-glutamine 생성)

  • 백선영;이혜경;최순영;김종욱;이세배;임번삼;민경희
    • Microbiology and Biotechnology Letters
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    • v.18 no.3
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    • pp.296-300
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    • 1990
  • For interspecific portoplast fusion, Brevibacterium flauum lOAHR (Rifr axg his) and Corynebacterium glutamicum 11TS ($Sm-r$ trp) were induced by UV and NTG treatment. The protoplast fusion frequency between E. flavum XOAHR and C. glutamicum llTS was $3.7\times 10^{-6}$ with the lysozyme treatment (300 P $\mu g$ml) for 18 hrs. Genotypes of recombinants were analized as FMM ($Rif^r\; Sm^r$), FA (Rift $Sm^r$ arg), FH ($Rif^r\; Sm^r$ his), FT ($Rif^r\; Sm^r$ trp), FAH ($Rif^r\; Sm^r$ arg trp), FAT ($Rif^r\; Sm^r$ arg trp), and FAHT ($Rif^r\; Sm^r$ arg his trp). FAH 1 produced 12 fold of glutamate production compared to parental type, E. flauum 10AHR. In glutamine productivity, it produced 2.6 fold to parental type, C. glutamicum 11TS. Production of glutamate or glutamine by recombinants was involved in the specific activities of glutamate dehydrogenase (GDH) and glutamine synthetase (GS), respectively.

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Characterization of a New Gene Resistant to Alkylating Agents and 3-Aminobenzamide When Knocked Out in Fission Yeast (분열형 효모에서 유전자 결실에 의해 알킬화제와 3-AMINOBENZAMIDE에 저항성을 나타내는 새로운 유전자의 특성 분석)

  • 박종군;차재영;황성진;박세근;김미영;백성민;최인순;이정섭
    • Journal of Life Science
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    • v.12 no.2
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    • pp.219-225
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    • 2002
  • The organization of eukayotic chromatin into specific conformation that are associated with transcription, replication, reapir and other nuclear processes are achieved via a series of DNA-protein interaction. These interactions are mediated by a range of DNA-binding domains such as SAP domain et at. By searching S. pombe genomic DNA database, we have found a gene named SAPuvs (SAP UV Sensitive) whose amino acid sequence is in part similar to SAP domain of Arabidopsis poly (ADP-ribose) polymerase and Ku7O. Knock-out cell of S. pombe SAPuvs gene was constructed using Ura4 as a selection marker. Survival analysis of knock-out cell indicated that treatment with UV significantly reduces the survival compared to wild type cell. Potentiation of MMS-induced cytotoxicity by 3AB post-treatment was observed in wild type cells, but not in knock-out cells. These data suggested that the protein encoded by SAPuvs gene is associated with chromatin reorganization during DNA repair.

Web-Building Strategy of a Wasp Spider, Argiope bruennichi, under Sensory Information Emitted by a Prey Species (먹이 종 감각 정보 제공에 따른 긴호랑거미(Argiope bruennichi)의 웹 건축 전략)

  • Jin, Woo-Young;Shin, Hyun-Chul;Kim, Kil-Won
    • Journal of Life Science
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    • v.21 no.7
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    • pp.1009-1015
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    • 2011
  • Web structure of spiders is a foraging strategy as well as an investment to get prey. In order to increase the fitness consequence, spiders change their foraging strategy based on sensory information provided by prey species. We conducted an experiment to demonstrate whether an orb-web building spider, Argiope bruennichi modifies its web-building behavior after experiencing sensory information emitted by a UV recognizing flying insect, Bombus terrestris. To know how the web structure would be modified, we observed web characteristics after providing sensory information of a potential prey species, the bumblebee (experimental group) and compared them to the control group (no information provided). We hypothesized that the spiders of the experimental group would increase investments in their web construction and stabilimentum decoration. The results rejected our hypothesis. Web and stabilimentum constructions decreased in the course of the experiment in both the control group and the experimental group. The individuals did not increase their stabilimentum length and did not extend their web areas, in spite of sensory information given by the nectar collector.

Production of Aminoglycoside-3'-Phosphotransferase by the Fed-Batch Cultivation of Mutant Obtained from E. coli ATCC 21990 (E.coli ATCC 21990 변이주의 유가배양법에 의한 Aminoglycoside-3'-Phosphotransferase 생산)

  • 김기태;김학주;김계원;나규흠;양중익;김수일
    • Microbiology and Biotechnology Letters
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    • v.19 no.5
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    • pp.491-496
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    • 1991
  • To maximize the production of aminoglycoside-3'-phosphotransferase of E. coli ATCC 21990 carrying R factor which encodes aminoglycoside-3'-phosphotransferase (APH(3')) phosphorylating the 3'-hydroxyl group of aminoglycoside, mutants M1 and M2, media composition and several factors affecting the enzyme production during fermentation were studied. Although the specific activity of APH(3') produced by a mutant M1 was increased as much as four times than that of E. coii ATCC 21990, the growth rate was decreased. The increase of the enzyme production was obtained by increased biomass during fermentation. A mutant M2 was obtained to increase the cell growth rate. Mutant M2 cells were cultivated with optimal media and pure oxygen gas in a fed-batch mode of fermentor operation. The specific activity of APH(3') was decreased, but total enzyme activity of APH(3') was increased as much as two point five times than that of mutant MI.

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Application of the Health Risk Models Estimating Skin Cancer Caused by UVB Radiation (자외선(UVB) 노출 증가에 대한 피부암 위해도 예측 모델의 적용)

  • Shin, Dong-Chun;Lee, Jong-Tae;Chung, Yong;Kang, Na-Kyung;Yang, Ji-Yeon
    • Environmental Analysis Health and Toxicology
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    • v.11 no.1_2
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    • pp.1-10
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    • 1996
  • A decrease in stratospheric ozone probably caused by chloroflurocarbons (CFCs) emissions, has been observed large parts of-the globe. It is generally accepted that if ozone levels in the stratosphere are depleted, greater amounts of shortwave ultraviolet radiationB (UVB) will reach the earth's surface, resulting in increased incidence of nonmelanoma skin cancer. In this study, we evaluated several mathematical models, such as a power and an exponential model, and a geometric model considering the surface area of a human body part and ages for the prediction of Skin cancer incidence caused by exposure to the UVB radiation. These models basically estimated the risk of skin cancer based on those measurements of the local ozone in stratosphere and UVB. Both were measured at a part of Seoul with a Dobson ozone spectrometer and Robertson-Berger UV Biometer for 1995. As a result, we calculated the point estimation applying a biological amplification factor (BAF), UVB radiation and other factors. We used a Monte-Carlo simulation technique with assumption on the distribution of each considered factor. The sensitivity analysis of model by there components conducted using Gaussian sensitivity method. The annual integral of UVB radiation was 2275 MED (minimal erythema dose)/yr. Also, an estimate of the annual amount of UVB reaching the earth's surface at a korea's latitude and altitude was 3328 MED/yr. The values of the radiation amplification factor (RAF) were ranged from 0.9 to 1.5 in Seoul. To give the effective factors required to model the prediction of skin cancer incidence caused by exposure to the UVB radiation in Korea, we studied the pros and cons of above mentioned models with the application of those parameters measured in Seoul, Korea.

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Action of Rodgersia podophylla Root Extracts on Melanin Biosynthesis in Skin (도깨비 부채 뿌리 추출물의 피부 과다색소침착에 미치는 영향)

  • Kong, Yeon-Hee;Lee, Pyeong-Jae;Choi, Sang-Yoon
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.6
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    • pp.434-436
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    • 2007
  • In the previous study, we reported the inhibitory effects of Rodgersia podophylla root extract on tyrosinase activity and melanin production in melan-a cells. However, mechanism of the inhibitory activity and in vivo assay were not yet examined. This study performed the examination of the effects of Rodgersia podophylla root extract on protein expression and in vivo depigmenting activity using melan-a cells and brown guinea pigs. As the results of western immunoblotting analysis, treatment of Rodgersia podophylla root extract reduced tyrosinase expression rates in 10 and 100 ppm concentrations, dose dependently. Moreover, Rodgersia podophylla root extract exhibited depigmenting activity on UV-B induced hyperpigmentation in brown guinea pig skin. These results suggested that Rodgersia podophylla root extract could act as whitening agent for the skin via not only direct tyrosinase activity inhibition but also reducing of tyrosinase expression.

Analysis of Browning Factors During Fermentation of Kochujang (고추장 발효 중 갈변 요인에 대한 분석)

  • Kim, Moon-Sook;Ahn, Yong-Seon;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.1149-1157
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    • 2000
  • To confirm the factors concerning color changes of traditional kochujang, heating, U.V. and $N_2$ gas substitution treatment were conducted on duk(rice cake) kochujang and shikhae(malt digested syrup) kochujang. The value of L, a and b value by Hunter of each kochujang were gradually decreased during fermentation and ${\delta}E$ increased. The ${\delta}E$ value of duk kochujang treated by U.V. was higher than those of the other treatments and non heated shikhae kochujang showed higher ${\delta}E$ value. The main cause of color change by factor analysis on various factors of duk kochujang and shikhae kochujang were light, and light and heat respectively. The acetone and water extracted pigments had maximum absorbance at 450-470 nm and 200-205 nm respectively.

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Color Characteristics of 3D-Printed TPU Material Applied with Ultra-Violet Curable Digital Printing Process (자외선 경화형 디지털 프린팅을 이용한 3D 프린팅 TPU 소재의 색채 특성)

  • Lee, Sunhee;Park, Soyeon;Jung, Imjoo;Lee, Jungsoon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.45 no.6
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    • pp.1052-1062
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    • 2021
  • This study aims to confirm the possibility of Ultra-Violet (UV)-printed 3D printing materials using thermal polyurethane (TPU) with CMYK colors by applying an eco-friendly UV digital printing process. A UV-printed 3D printing TPU material was prepared with cycles of UV printing and CMYK colors. Dyeability of the 3D TPU samples with cycles of UV printing and CMYK were analyzed for thickness, weight, surface roughness, reflectance, colorimetry, and K/S values. The thickness and weight of 3D-printed TPU samples with cycles of UV printing are increased with overprints from 1 to 5. The surface roughness of 3D-printed TPU samples with increasing UV prints were decreased, meaning that the surface of TPU samples becomes gradually smoother. The reflectance spectra of CMYK UV-printed TPU samples showed the surface reflectance within each characteristic wavelength of CMYK. The 3D-printed TPU samples, subjected to UV printing twice or more, showed low surface reflectance. After examining the L*a*b* of the 3D-printed TPU samples by the cycles of UV printing, the study found that the more UV got printed more than 2 times, the closer the color to each CMYK.