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http://dx.doi.org/10.5352/JLS.2002.12.2.219

Characterization of a New Gene Resistant to Alkylating Agents and 3-Aminobenzamide When Knocked Out in Fission Yeast  

박종군 (원광대학교 자연대 생명과학부)
차재영 (원광대학교 자연대 생명과학부)
황성진 (원광대학교 자연대 생명과학부)
박세근 (원광대학교 자연대 생명과학부)
김미영 (원광대학교 자연대 생명과학부)
백성민 (원광대학교 자연대 생명과학부)
최인순 (신라대학교 자연대 생물과학부)
이정섭 (조선대학교 자연대 샘영과학과)
Publication Information
Journal of Life Science / v.12, no.2, 2002 , pp. 219-225 More about this Journal
Abstract
The organization of eukayotic chromatin into specific conformation that are associated with transcription, replication, reapir and other nuclear processes are achieved via a series of DNA-protein interaction. These interactions are mediated by a range of DNA-binding domains such as SAP domain et at. By searching S. pombe genomic DNA database, we have found a gene named SAPuvs (SAP UV Sensitive) whose amino acid sequence is in part similar to SAP domain of Arabidopsis poly (ADP-ribose) polymerase and Ku7O. Knock-out cell of S. pombe SAPuvs gene was constructed using Ura4 as a selection marker. Survival analysis of knock-out cell indicated that treatment with UV significantly reduces the survival compared to wild type cell. Potentiation of MMS-induced cytotoxicity by 3AB post-treatment was observed in wild type cells, but not in knock-out cells. These data suggested that the protein encoded by SAPuvs gene is associated with chromatin reorganization during DNA repair.
Keywords
S. pombe; MMS (methylmethane sulfonatde); UV; 3AB (3-Aminobenzamide); PARP (poly (ADP-ribose)polymerase); SAP (SAF-A/B, Acinus and P-IAS) domain;
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