• Journal of the Korean Applied Science and Technology
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• v.38 no.3
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• pp.750-761
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• 2021

This study was performed to observe the responses of angiogenesis-related protein expression in skeletal muscle of aged rats by regular resistance exercise training with aerobic exercise. For the purpose of the study, naturally aged SD rats (20-24 months, N=18) were used and divided into control (CON, n=6), resistance exercise (RE, n=6), and resistance + aerobic exercise (RE + AE, n=6) groups. RE group performed 3 sets × 4 exercises each session using a ladder for laboratory animals, and RE +AE group performed 2 sets × 3 times of ladder climbing and additional treadmill running (30 min) each session. After 8 weeks of exercise training, soleus muscle and extensor digitorum longus muscle (EDL) were extracted and used for analysis. Western blot was performed to analyze the expression levels of angiogenesis-related proteins (HIF-1α, VEGF, FLK-1, Ang-1, Ang-2) in skeletal muscle. As a result of the study, the expression of HIF-1α, VEGF, FLK-1, Ang-1, and Ang-2 proteins in soleus muscle (type I muscle) was higher in RE +AE than in CON group, and HIF-1α, VEGF, Ang-1, Ang-2 protein expression of RE group was higher than that of CON group. Furthermore, Ang-2 to Ang-1 ratio of RE + AE group was higher than that of RE group, showing differences by exercise type. In EDL muscle (type II muscle), HIF-1α was increased only by RE group, whereas VEGF and FLK-1 protein expressions were increased in both training types, and no difference was observed between the types of exercise training. In addition, there was no difference in angiopoieitin protein expressions in EDL muscle by exercise training. Therefore, in aging, regular exercise training induces skeletal muscle angiogenic response regardless of exercise type, and in particular, the combination of aerobic exercise with resistance exercise may have an additional positive effect on angiogenesis in type I muscle.

• Journal of Life Science
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• v.33 no.3
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• pp.277-286
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• 2023

Sarcopenia is the age-related loss of muscle mass and function. It is a natural part of aging and can lead to decreased mobility and increased frailty. The ubiquitin-proteasome pathway, which is involved in muscle protein degradation, is closely linked to sarcopenia. Germinated Rhynchosia nulubilis hydrolysate (GRH) has been reported to have anti-inflammatory and antioxidant properties, but there have been no reports on its inhibitory effect on muscle reduction. However, no study has yet explored the relationship between GRH and muscle loss inhibition. In this study, we evaluated the effects of GRH on muscle atrophy inhibitory activity in dexamethasone (Dexa)-induced muscle atrophy C2C12 myotubes and mouse models. Moreover, we identified a molecular pathway underlying the effects of GRH on skeletal muscle. May Grunwald-Giemsa staining showed that the length and area of myotubes increased in the groups treated with GRH. In addition, the GRH-treated group significantly reduced the expression of muscle ring finger protein 1 and muscular atrophy F-box (MAFbx) in the Dexa-induced muscular atrophy C2C12 model. GRH also improved muscle strength in C57BL/6 mice with Dexa-induced muscle atrophy, resulting in prolonged running exhaustive time and increased grip strength. We found that muscle strengthening by GRH was correlated with a decreased expression of the MAFbx gene in mouse muscle tissue. In conclusion, GRH can attenuate Dexa-induced muscle atrophy by inhibiting the ubiquitin-proteasome pathway via downregulation of the MAFbx gene expression.

• The Journal of Natural Sciences
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• v.18 no.1
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• pp.39-46
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• 2007

Three kinds of plant fermentation broths and one liquor were prepared using persimmon, Ulmus davidiana var. japonica and barley, and investigated its physiological functionality. Anti-aging antioxidants activity was the highest of 99% in Ulmus dacidiana bar. japonica liquor and also was high in the persimmon vinegar(68.3 %). SOD-like activity show also 80.6% in the Ulmus davidiana var japonica liquor however the other fermentation broths were less 25%. Antihypertensive angiotensin I-converting enzyme inhibitory activity and fibrinolytic activity were weak or not detected in all the fermentation broths. Anti-dementia acetylcholinesterase inhibitory activity were showed 54.7% in the Ulmus davidiana var. japonica liquor. Our results showed that the Ulmus davidana var. japonica liquor has the potential to become a new functional liquor because of high antioxidant activity and anti-dementia activity.

• Microbiology and Biotechnology Letters
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• v.6 no.4
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• pp.181-185
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• 1978

The competitions were made between two kinds of Kochujang, one is the native fermented Kochujang which is fermented at 20$\pm$2$^{\circ}C$, and the other is quick fermented Kochujang which is maintained at 35$\pm$2$^{\circ}C$ for 30 days. The results were showed that the general compositions, amino acid, nitrogen and flavor of quick fermented Kochujang were superior to the native fermented Kochujang.

• 한국HCI학회:학술대회논문집
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• 2007.02a
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• pp.841-847
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• 2007

최근 삶의 수준의 향상과 의학 기술의 발전으로 노인 인구가 증가하고 있다. 하지만 늘어나는 노인 인구에 비례하여 신체적 노화로 거동이 어려운 노인의 수 또한 증가하는 추세이다. 실제로 많은 노인 인구가 거동이 불편해 정상적인 생활을 하지 못하고 있기 때문에 보행 시 적절한 힘을 보조해 줄 수 있는 보행 보조 장치의 개발이 필요하다. 이 같은 보행 보조 장치를 개발함에 있어 보행자의 보행 패턴이 고려된다면 보행자의 걸음걸이에 맞춰 자연스럽게 힘을 보조해 줄 수 있기 때문에 보행자의 보행 단계 분류에 관한 연구가 선행되어야 한다. 그래서 본 논문에서는 하지 근전도 신호를 이용해 보행 단계를 구분하는 방법을 제안하고자 한다. 근전도 신호는 근육이 움직일 때 발생하는 아주 작은 전기적인 신호이다. 근전도 신호는 작은 잡음에도 민감하며, 전극을 부착하는 근육의 위치에 따라서도 값의 차이가 크기 때문에 근전도 신호의 획득 및 처리 방법이 중요하다. 위를 위해 피실험자 별 근육의 위치와 보행 속도를 달리하여 근전도 신호를 획득하고 획득한 신호로부터 여러 특징 값을 추출한다. 그리고 새로운 데이터에 대해 적응성이 강하고 시간에 따라 변하는 근전도 신호의 특성을 잘 반영할 수 있으며 각 집합(class)의 비선형 분리가 가능한 퍼지 최대-최소 신경망(Fuzzy Min-Max Neural Network: FMMNN)을 이용해 보행 단계를 분류해 본다. 실험 결과를 통해 제안한 방법의 타당성을 검증해 보고 보행자, 보행속도, 근전도 측정을 위한 근육의 위치가 보행 패턴 분류에 미치는 영향을 알아본다.

• Journal of Hydrogen and New Energy
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• v.23 no.3
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• pp.256-263
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• 2012

We studied the degradation phenomenon of Pt catalyst in PEMFC. We used the electron microscope analysis technique including the ultra-microtome pretreatment method, FEG-SEM and TEM analysis methods for analysis of Pt nanoparticles. The Pt catalyst degradation is observed not only in electrode site but also in membrane site. We investigated these various degradation phenomena. The cathode electrode layer thickness is reduced. The size of the catalyst is increased much larger than initial size in membrane site. The catalyst moved from electrode layer to the electrolyte membrane. The rounded shape of catalyst was changed to the polygon. As a result, we found that the catalyst degradation processes of migration and coarsening occurred by the followings mechanisms; (1) dissolution of Pt ; (2) diffusion of Pt ion ; (3) Pt ion chemical reduction in membrane; (4) Coarsening of Pt particles (Ostwald ripening) ; (5) polygon shape change of Pt by {111} plane growth.

• Journal of Integrative Natural Science
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• v.16 no.2
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• pp.61-67
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• 2023

Human body ages differently due to environmental, genetic and pathological factors. DNA methylation patterns also differs depending on various factors such as aging and several other diseases. The aging clock model, which uses these differences to predict age, analyzes DNA methylation patterns, recognizes age-specific patterns, predicts age, and grasps the speed and degree of aging. Aging occurs in everyone and causes various problems such as deterioration of physical ability and complications. Alzheimer's disease is a disease associated with aging and the most common brain degenerative disease. This disease causes various cognitive functions disabilities such as dementia and impaired judgment to motor functions, making daily life impossible. It has been reported that the incidence and progression of this disease increase with aging, and that increased phosphorylation of Aβ and tau proteins, which are overexpressed in this disease and accelerates epigenetic aging. It has also been reported that DNA methylation is significantly increased in the hippocampus and entorhinal cortex of Alzheimer's disease patients. Therefore, we calculated the biological age using the Epi clock, a pan-tissue aging clock model, and confirmed that the epigenetic age of patients suffering from Alzheimer's disease is lower than their actual age. Also, it was confirmed to slow down aging.

• Journal of Life Science
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• v.34 no.7
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• pp.476-484
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• 2024

Although two-dimensional (2D) monolayer cell culture models are still widely used as the optimal models for anticancer activity research, three-dimensional (3D) multicellular tumor spheroid (3D MTS) models that can better approximate the tumor environment can offer an alternative to bridge the gap between in vitro and animal model studies. Isoalantolactone is among the sesquiterpene lactones found in medicinal plants, including the roots of Elecampane (Inula helenium L.), and is known to have various pharmacological activities, including anticancer activity. In this study, we investigated whether the anticancer activity of isoalantolactone observed in 2D models could be reproduced in a 3D MTS model derived from human hepatocellular carcinoma (HCC) Hep3B cells. According to our results, isoalantolactone inhibited the formation of MTSs in a manner dependent on the treatment concentration, which was accompanied by an increase in reactive oxygen species (ROS) generation. In particular, as isoalantolactone treatment and the culture time increased, the area of proliferating cells was replaced by cells in which apoptosis was induced. Additionally, in MTSs, isoalantolactone increased the expression of death-receptor-related proteins and the activity of caspase-3, and it decreased the expression of the Bax/Bcl-2 expression ratio and total poly(ADP-ribose) polymerase. However, when the production of ROS was artificially blocked, all these changes caused by isoalantolactone were attenuated and the cell survival rate of MTS cells was restored. Therefore, the results of this study suggest that the induction of apoptosis in Hep3B cell-derived MTSs by isoalantolactone is achieved through the activation of extrinsic and intrinsic pathways and is ROS-dependent.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.7
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• pp.4272-4278
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• 2014

One of the most effective ways of preventing skin aging is to protect the skin from UV radiation, which was identified as the primary cause of photoaging. Therefore, it is necessary to develop natural and environment-friendly materials to the human skin. This study examined the effects of MAAs extracted from Chlamydononas hedleyi on UV protection and anti-inflammation in human skin cells. The function of porphyra-334 in the skin, which was isolated and purified from MMAs mixture, was tested in terms of its UV protective ability and anti-inflammation. As a result, porphyra-334 played a role in protecting the skin from UV radiation and anti-inflammation through the suppression of COX-2 expression. These results suggest that porphyra-334 can be a useful material in cosmetic products because it can protect the skin from UV radiation and anti-inflammation.

• 한국노년학
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• v.29 no.4
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• pp.1665-1683
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• 2009

The purpose of this study is to examine what the formation of a community incorporating two generations of people can give the elderly and the problems which are associated with establishing related programs of this kind. For this, the researcher enacted ethnographic method like as observant participation and in-depth interview on 24 participants. From this study, I found that the elderly and the children who took part in several programs to form the intergenerational linkages made the system meaningful in the following ways: first, the more harmonious the communication across between two age groups is, the more the understanding between them increases. Second, the sense of community has intensified the natural harmony. Third, the more self-satisfaction and confidence increases, the more self-efficacy is enhanced. Fourth, the purposeful and creative activities with peers have enabled the elderly to enjoy their leisure time. Fifth, the elderly have experienced the pleasure of learning and sharing common sense as a life-long learners. However, in the process of this program, several problems occurred such as the rigidly bureaucratic operation of the program and the elderly people's individual differences. In addition, the lack of a precisely-existing program necessary to form the intergenerational linkages and to bring together different generations was a problem. Finally, I have concluded that the effort to form the intergenerational linkages helps increase the understanding and cooperation across age groups and contributes to the successful aging of the elderly.