• The Korea Journal of Herbology
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• v.32 no.6
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• pp.31-39
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• 2017

Objectives : Menopausal symptoms are characteristic symptoms which are occur in women before and after menopause. In Western medicine, hormone therapies are mainly used but patients show reluctance because they exhibit serious side effects. In Oriental medicine, there are also limits to the treatment. So I tried to find a new easily accessible treatment. I performed the experiments to verify the effect of natural dyed apparel with Indigo and Schisandra fruit. Methods : This experiment was performed on 30 women who were experiencing menopausal symptoms. We checked the participant's general condition through questionnaire and physical condition with inbody test and made natural-dyed living suit style dresses. We asked the women to wear the apparel for four weeks. They checked for changes in symptoms weekly with kupperman's index. Results : The main symptoms of participants are sweating, hot flushes, joint pain. After wearing of the apparel, there were decreased hot flushes, sleep improvement, psychological stability etc. According to the kupperman's index, patients with mild symptoms were 13.3% at first week to start the experiment but four weeks later, patients with mild symptoms were increased to 50%. On the other hand, patients with severe symptoms were decreased 33.3% to 6.7%. In the test results, there was a significant decrease in 1, 2 and 3 weeks, there was a decrease in 4 weeks but no significance in the figure Conclusion : Natural dyed apprel with Indigo and Schisandra fruit was effective on treating women who were experiencing menopausal symptoms.

• Proceedings of the Korean Society of Disaster Information Conference
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• 2023.11a
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• pp.295-296
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• 2023

2023년 우리나라에 발생한 재난은 남부지방의 가뭄, 태풍 카눈에 의한 폭우, 7월~9월 폭염 등의 자연재난과 4월 5일 분당 정자교 붕괴, 4월 29일 검단신도시 붕괴사고, 7월 13일 대구 염색산업단지 황산 누출 등의 사회재난이 발생하였다. 이전 2022년에는 022년 10월 29일, 서울 이태원역 부근에 대규모 인파가 한번에 몰리며 압사사고가 발생하여 159명이 목숨을 잃고 196명의 부상자가 발생하였다. 이처럼 다양한 형태로 발생하는 재난에 대하여 우리나라는 「헌법」과 「재난 및 안전관리 기본법」에서 재난으로부터 국민의 생명·신체 및 재산과 국가의 피해를 줄이기 위해 노력하여야 한다고 명시하고 있다. 재난은 다양한 특성을 지니고 있으며, 학자들의 일반적 견해는 누적성, 불확실성, 복잡성, 인지성 등의 특성을 들고 있다(재난관리론, 2020). 특히, 사회재난은 발생위치, 시점, 피해, 규모를 예측하기가 상대적으로 매우 어렵기에(장대원 외, 2019) 불확실성의 특성을 강하게 보여주고 있다. 이러한 사회재난의 특성은 자연재난과 비교하여 사람들에게 물리적·심리적 영향을 크게 미칠 뿐만 아니라 단기간에 회복하기 어려운 특성을 지닌다(신재헌 외, 2020). 이에 대하여 많은 국민들은 심각한 사회재난이 발생할 때마다 정부차원의 적극적 재난관리 및 통제능력 향상을 요구하고 있으나, 사회재난 특성 상 특정 기관 중심으로 대응하기에는 많은 어려움이 있다(신재헌 외, 2020). 우리나라 경찰은 2021년 7월부터 자치경찰제도를 실시하며 국민의 생명·신체 및 재산의 보호, 범죄예방, 수사 및 질서유지업무 이외에 국민들의 봉사·서비스 행정 요구의 증대로 인한 적극적·능동적 경찰활동으로 변화하게 되었다(조호대, 2014). 이에 따라 경찰은 경찰의 재난관리체계를 확립하고, 경찰의 재난관리에 관한 사항을 규정함을 목적으로 2012년 「경찰 재난관리규칙」을 제정하였다. 이에 본 연구에서는 경찰의 재난 발생 시 역할이 무엇이며, 특히 사회재난 시 경찰의 적극적 대응 방안은 무엇인가에 대하여 살펴보고자 한다.

• Korean Journal of Veterinary Research
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• v.37 no.4
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• pp.825-829
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• 1997

The spleen and placenta from the aborted fetuses as well as lymphnodes and placenta of the corresponding dams naturally infected with T sergenti were used to localize the parasite antigens by immunohistochemical staining for the possible congenital transmission of theileriosis. Parasite-specific antigens were detected immunohistochemically by incubating the sections with specific monoclonal antibody prepared against 34KD surface antigen of T sergenti and visualized via the avidin biotin complex(ABC) method. Specific T sergenti antigen was detected in the sections of formalin or acetone-fixed fetal spleens and placenta. Similar antigens were also demonstrated in lymphnodes and placentas of the corresponding dams. It is concluded that this technique will eventually play an important role in specialized diagnostic laboratories in the verification/evaluation of congenital infection with T sergenti.

• Microbiology and Biotechnology Letters
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• v.16 no.3
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• pp.182-186
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• 1988

The possibility of strain improvement of cellulolytic fungus, Penicillium verruculosum via protoplast fusion was investigated. The cellulolytic activities of the six fusants, finally selected for their hyper-cellulolytics were 2 times of those of wild type and 1.2 to 4.4 times of those parental auxotrophs. It was confirmed that the nuclear fusion occurred in fusants by their DNA contents and nuclear staining with Giemsa. It was also found that the fusants were aneuploids, and their genetic stability was demonstrated from the subculture for four months.

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.315-318
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• 1998

This study was conducted to clarify the acclimatization rate and ploidy level of anther culture-derived plants in broccoli. The acclimatization rate from 71.4 to 100% was obtained from 7 varieties in 2 years. It was possible to identify the ploidy of the Plants obtained through anther culture by measuring the number of chloroplast in the guard cell. The average numbers of chloroplasts per guard cell in haploid diploid, and tetraploid were 8.5, 13.5 and 18.5, respectively. The regenerated plants could be classified based on these results into 47.1-51.3% of haploids, 47.9∼51.7%, diploid, and 0.8∼l.2% of tetraploids.

• Korean Journal of Veterinary Research
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• v.24 no.2
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• pp.237-243
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• 1984

한국에서 돼지에 감염되고 있는 Eperythrozoon 증양 질병의 역학적 생물학적 기초연구를 위해 본 질병에 자연감염 발병한 돼지 157두와 정상인 돼지 240두에 대하여 조사하였던 바 다음과 같은 결과를 얻었다. 혈액표본에서 관찰한 본 병원체의 감염율 형태 및 크기는 계절에 따라 변화를 보였는데, 정상인 돼지는 년평균 54% 감염율을 발병한 돼지는 모두 감염된 상태였다. 5종의 형태를 보이는 본병원체는 크기가 $0.5{\sim}0.5{\mu}$이었는 데 감염 혈액을 $0^{\circ}C$에 1주 이상(혹은 $-20^{\circ}C$에 24시간) 냉장하는 경우 그 크기가 $1.4{\sim}8.5{\mu}$까지 커졌으며 세포질내에 염색질 과립을 보이는 예도 발견되었다. 본 병원체는 적혈구막에 부착되거나 혈장내 유리된체 관찰되었으며 병든 돼지의 위장관과 방광내용물 중에서도 다수의 병원체가 발견되었다. 인공감염 실험에서 접종 감염된 돼지는 2개월간 경도의 체온상승과 빈혈상을 나타내면서 혈액내에 전형적인 병원체를 보유하였으며 접종시킨 쥐에서는 12개월이상 동일한 병원체의 잠복 감염상태를 유지하였다. 또한 병든 돼지 주위에서 포획한 파리와 모기의 장내용물 중에서 전형적인 본 병원체를 관찰할 수 있었다.

• Proceedings of the Korean Society for Quality Management Conference
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• 2006.04a
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• pp.367-374
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• 2006

시화호 개발 시점은 1975년 농업진흥공사가 시화호 유역을 간척농지로 개발한다는 계획을 수립한 시점부터 잡을 수 있으나 건설교통부가 이 지역을 반월특수지역 개발구역으로 지정한 1986년 9월 27일로 보는 것이 타당할 것으로 본다. 1986년에 정부가 그렇게 지정한 목적은 이 지역을 방조제로 막아서 담수호를 만들어 농업용수를 공급하고 농지 조성과 수자원 확보를 기한다는 목적 및 그에 따른 수도권 인구 분산 및 도서 지역 균형 개발을 이루겠다는 것이었다. 그러나 1995년 4월 2억4천백85만 톤의 썩은 시화호 물을 서해로 당류하고 바닷물을 유입한 이래 2001년 2월 정부가 다시 해수호로 인정하기까지 이 호수는 숟한 우여 곡절과 정부의 잦은 정책 변경으로 자연 환경이 파괴되었으며 생태계 변화에 따른 인근 지역과 농작물에 많은 피해를 보았고 염색 공단에서 흘러나온 폐수로 인해 악취를 풍기는 등 오염이 심각해 완전히 실패로 돌아간 '환경실패대작'이라고 할 수 있다. 이렇게 된 데는 정책 집행 과정에서 환경오염 저해 요인에 대한 무사 안일한 대처, 비밀주의, 철저하지 못한 오폐수의 단속 등 정부 폭의 실책을 우선해서 말할 수 있다. 본 논문에서는 이런 것을 포함해서 시화호가 실패하게 된 여타 중요 요인에 대해서 알아보고 또 지속 가능한 활용방안 및 대책에 대해서 간략히 알아보고자 한다.

• Development and Reproduction
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• v.15 no.4
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• pp.373-383
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• 2011

Tributyltin (TBT) is one of endocrine disrupters which are known as having similar function to sex steroid hormone inducing apoptosis in various tissues of rodents. Recently, it has been reported that TBT induces apoptosis in thymus causing the decreased thymic function, but little is known about the mechanism. To elucidate the mechanism, three-week-old SD female rats were orally administrated with TBT 1, 10, and 25 mg per body weight (kg) and sesame oil as a control for 7 days. On day 8, the thymi were obtained and weighed, and then the number of thymocytes was counted. We also performed H&E staining, TUNEL assay, and Annexin V flow cytometric analysis to examine the apoptosis rates and the structure in the thymus. Next, we investigated the adipogenesis and apoptosis-related mRNA expression levels in the thymi by real-time PCR. The thymic weight and the number of thymocytes were decreased by TBT in a dose-dependent manner. As a result of the H&E staining, the boundary between cortical and medullary area was blurred in the thymi of TBT treated rats compared to those of controls. In the results of TUNEL assay and Annexin V flow cytometric analysis, apoptosis rates in the thymus were increased after TBT treatment. The expression levels of thymic epithelial cell marker genes such as EVA, KGF, AIRE, and IL-7 were significantly decreased in the thymi of TBT treated rats, but $PPAR{\gamma}$, aP2, PEPCK, and CD36 were significantly increased. The expression of $TNF{\alpha}$ and TNFR1 as apoptosis-related genes also was significantly increased after TBT treatment. The present study demonstrates that TBT can increase the expression of adipogenesis and apoptosis-related genes leading to apoptosis in the thymus. These results suggest that the increased adipogenesis of thymus by TBT exposure might induce apoptosis in the thymus resulting in a loss in thymic immune function.

• Journal of Life Science
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• v.12 no.4
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• pp.452-459
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• 2002

Neutrophil apoptosis is a constitutive process that can be enhanced or delayed by various stimuli. In this study, effect of brefeldin A (BFA), which affects biological process of secretion, on constitutive and delayed apoptosis of neutrophils was investigated. Neutrophil apoptosis was determined after culturing for 20 hr in vitro by morphological changes, annexin V staining and DNA electrophoresis. BFA increased the constitutive apoptotic rate of neutrophils in dose-dependent manner. The delay of apoptosis induced by granulocyte macrophage-colony stimulating factor and lipopolysaccharide was also blocked by 10 $\mu$M of BFA. However, this effect of BFA was less marked when neutrophils were treated with dexamethasone, interleukin-8, or dibutyryl-cAMP. Moreover, the delay of neutrophil apoptosis induced by rottlerin, a specific inhibitor of protein kinase C-$\delta$ was significantly abrogated by BFA. Although BFA-induced apoptosis was not blocked by the caspase-3 inhibitor, zDEVD-fmk, expression levels of myeloid cell leukemia-1 (Mcl-1) were down-regulated by BFA. These results suggest that derangement of vesicular protein transport may be involved in the apoptosis of neutrophils, and that the action of BFA on apoptosis is dependent on changes in the expression of Mcl-1.

• Journal of Ginseng Research
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• v.30 no.4
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• pp.206-211
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• 2006

To investigate mycorrhizal infection by arbuscular mycorrhizal fungi(AMF), samples of fine lateral roots were taken from the wild ginseng(Panax ginseng C.A. Meyer) naturally growing at various locations in Korea. Mycorrhiazal infections were studied by cleaning the root samples and staining fungal hyphae with frypan blue. Wild ginsengs for this study were graded by an appraisal committee consisting of 12 experts of Korea Mountain Ginseng Association. Following five quality groups were recognized: Heaven group(pure natural), Earth group (from seeding of wild ginseng), Man group(from seeding or seedlings of wild ginseng with slight environmental modification), unmarketable, and imported wild ginseng. Morphology of AMF was typical Paris-type which shows intracellular hyphal coils with rare vesicles and lack of arbuscules. Average infection rate of individual wild ginsengs was 58.3% and showed no differences among five quality groups. When portions of fine roots were quantified for mycorrhizal infection, 18.7% of the total length of the primary and secondary roots were infected by AMF. Wild ginsengs from Gyeonggi Province(84.2%), and from mountains lower than 1,200 meters above sea level(about 70%) showed higher infection rate, while the ginseng from Gyeongbuk Province(27.8%) had lower rate. Wild ginsengs at older age showed lower infection rates.