• Asian Journal of Turfgrass Science
• /
• v.12 no.3
• /
• pp.237-248
• /
• 1998

The soil pH favored by Korean wild plants is 5.33~7.20, while naturalized invader species prefer soils of pH 3.95~6.48. The germination rate of Zoysia japonica was inhibited sharply, up to 60%, in extracts of naturalized plants of concentrations over 50%. Erigeron canadensis extract most strongly inhibited germination, while the Korean wild species, Cassia mimosoides var. nomame increased germination rates in concentrations of 30% and 50%. The seedling growth of Zoysia japonica in extracts of Korean wild species and naturalized species did not show differences, but was inhibited strongly, up to 60%, in the extract of Cassia mimosoides var. nomame concentration of over 15%. Analysis of the extract from Cassia mimosoides var. nomame identified procatechuic acid, $\rho$-hydroquinone, $\rho$-coumaric acid and ferulic acid at 254nm; and vanillic acid, hydroquinone, benzoic acid and cinnamic acid were discovered at 284nm. The seed germination and seedling growth of Zoysia japonica were investigated under different concentrations of phenolic compounds. Ferulic acid and vanillic acid were associated with an increased germination rate, while $\rho$-coumaric acid appeared to inhibit seedling growth.

• Weed & Turfgrass Science
• /
• v.2 no.1
• /
• pp.55-61
• /
• 2013

This study was carried out to investigate the morphological and soil characteristics of Zoysia spp. native to South Korea. Samples [41 ecotypes of Zoysia japonica, 28 ecotypes of natural hybride between Z. japonica and Z. sinica (Junggi), 22 ecotypes of Z. sinica, and 8 ecotypes of Z. matrella] were collected in seashores, levee and summit of the rock in southern Korea. Variations in leaf width, plant height, leaf angle, length of leaf sheath, leaf trichome, stolon length, and seed shape were measured at collection sites and experimental plots. Among the entries, most of the measurements did not show any significant differences between natural and experimental plot except for leaf angle of Z. sinica and the number of seeds per spike of Z. matrella, which might be caused by different environmental conditions. Soil pH was 6.0 at the most of the collection sites. $Mg^+$ was 0.06-0.02 $cmol{_c}^+kg^{-1}$, $Mg^{2+}$ was 0.09-0.03 $cmol{_c}^+kg^{-1}$, and $K^+$ was 0.02-0.007 $cmol{_c}^+kg^{-1}$ at most zoysiagrasses growing soils.

• Asian Journal of Turfgrass Science
• /
• v.25 no.2
• /
• pp.138-146
• /
• 2011

This study was carried out to select salt tolerant zoysiagrass breeding lines. Eighty two native zoysiagrasses collected from S. Korea were used in this study. Saline water were prepared by mixing sea water and tap water. ECw levels of saline water treated ranged from 2 to $3dS{\cdot}m^{-1}$. Zoysiagrass planted in pot by sprigging were soaked into the plastic box containing saline water. Winter injury was investigated under the pot condition. Most of Z. japonica types did not show winter injury. But Z. tenuifolia type, Z. matrella type, and Z. sinica type showed winter injury under the pot condition at Cheonan area. NaCl level in soil was increased from 0% to 0.51% by treatment of saline water. Soil ECe measurement showed upto $170dS{\cdot}m^{-1}$. Z. tenuifolia type (Z5034), Z. matrella type ('Konhee', Z4109, 'Semill'), Z. japonica type (Z1055, Z1040, Z1008, 'Zenith', 'Millock') and medium leaf type zoysiagrass (Z6096, Z6118, Z6021, Z6074) resulted in below 30% leaf firing under the saline condition. This approach might be useful for selecting salt tolerant breeding lines.

• Weed & Turfgrass Science
• /
• v.6 no.2
• /
• pp.136-150
• /
• 2017

Zoysiagrass mite, Aceria zoysiae is one of the main turfgrass pests causing significant damage on zoysiagrass. In this study we investigated damage by zoysiagrass mite for the selection of zoysiagrass mite-resistance turfgrass individual in natural collected turfgrass isolates transplanted in genetic resources conservation area. In the results of investigation of 295 turfgrass isolates collected from Korea, damage level was different depending on turfgrass species and individuals. Zoysia japonica was highly damaged representing 97.6%, followed by hybrid zoysia and Z. sinica with damage percentages of 87.7% and 81.1% respectively. Additionally, 2 individuals of Z. macrostachya were also damaged by zoysiagrass mite. However several individuals were not damaged by zoysiagrass mite in green house and field. Damage by zoysiagrass mite was occurred from May and peak at after July in field. The study highlighted some important resistant turfgrass individuals which can be used in the management of zoysiagrass mite.

• Weed & Turfgrass Science
• /
• v.2 no.2
• /
• pp.184-190
• /
• 2013

It is important for genetic resources to collect and identify in native medium-leaf type zoysiagrasses species distributed in Korea. This study was conducted to investigate morphological variation and characteristics of native medium-leaf type zoysiagrasses from coastal, island and inland regions in Korea. Among them, 75 collected lines was confirmed to have various morphological variations, accessions were classified into 2 main based group coastal and inland regions by morphological characteristics and site environment. Group I included Z. sinica type, this group showed 3.7 mm in leaf width, 29 in number of seed per spikelet and 5.0 mm in seed length. Group II included Z. japonica type, this group showed 4.4 mm in leaf width, 42 in number of seed per spikelet and 3.5 mm in seed length. There is a need for additional research on growth characteristics and the molecular level for the introgressive hybridization between species which confirmed that cross-pollination is possible due to protogyny. The individuals showing variations should be preserved as valuable genetic resources for the expansion of variations in zoysiagrasses, and the results of this investigation on the genetic resources collected will be highly valuable in breeding high quality turfgrass.

• Asian Journal of Turfgrass Science
• /
• v.24 no.2
• /
• pp.97-105
• /
• 2010

This study focuses on investigating morphological characteristics and variations level in native zoysiagrasses from costal regions and islands in South Korea. In addition, this were collected to conserve germplasm for the breeding of zoysiagrass. One hundred fifty-nine of native zoysiagrasses were collected from 16 different cities and districts. There were morphological evaluations, including plant height, leaf width, leaf length, leaf angle, seed length(SL), seed width(SW), SL/SW ratio and number of seed per spike in native zoysiagrasses. Type of Z. japonica, Z. sinica, Z. macrostachya and Z. matrella showed significant variations in plant height, leaf length, leaf angle and number of seed per spike depending on where they were collected from. On the other hand, little variation were found in leaf width, seed length, seed width and SL/SW ratio. We have discovered a variety of variations among the investigated traits in hybrid zoysiagrass. These results can be used as basic information for development of zoysiagrass breeding.

• Horticultural Science & Technology
• /
• v.35 no.3
• /
• pp.344-360
• /
• 2017

Zoysiagrasses are important turf plants used for school playgrounds, parks, golf courses, and sports fields. The two most popular zoysiagrass species are Zoysia japonica and Zoysia sinica. These are widely distributed across different growing zones and are morphologically distinguishable from each other; however, it is phenotypically difficult to differentiate those that grow along the coastal line from those in beach area habitats. A combination of morphological and molecular approaches is desirable to efficiently identify these two plant cultivars. In this study, we used a rapid identification system based on DNA barcoding of the nrDNA-internal transcribed spacer (ITS) regions. The nrDNA-ITS regions of ITS1, 5.8S nrDNA, and ITS2 from Z. japonica, Z. sinica, Agrostis stolonifera, and Poa pratensis were DNA barcoded to classify these grasses according to their molecular identities. The nrDNA-ITS sequences of these species were found at 686 bp, 687 bp, 683 bp, and 681 bp, respectively. The size of ITS1 ranged from 248 to 249 bp, while ITS2 ranged from 270 to 274 bp. The 5.8S coding region ranged from 163 - 164bp. Between Z. japonica and Z. sinica, nineteen (2.8%) nucleotide sites were variable, and the G+C content of the ITS region ranged from 55.4 to 63.3%. Substitutions and insert/deletion (indel) sites in the nrDNA-ITS sequence of Z. japonica and Z. sinica were converted to cleaved amplified polymorphic sequence (CAPS) markers, and applied to the Zoysia grasses sampled to verify the presence of these markers. Among the 62 control and collected grass samples, we classified three groups: 36 Z. japonica, 22 Z. sinica, and 4 Z. japonica/Z. sinica hybrids. Morphological classification revealed only two groups; Z. japonica and Z. sinica. Our results suggest that used of the nrDNA-ITS barcode region and CAPS markers can be used to distinguish between Z. japonica and Z. sinica at the species level.

• Weed & Turfgrass Science
• /
• v.5 no.2
• /
• pp.95-100
• /
• 2016

Seeded variety of zoysiagrass has not been bred yet in Korea. Development of seeded zoysiagrass cultivar will be very important for the growth of turfgrass industry internationally as well as domestically. This research was conducted to investigate seed yield and germinability of 102 collected native zoysiagrass ecotypes in South Korea. Two hundred and seventy seven ecotypes were collected from various locations including coastal and mountain areas, while 102 morphologically distinct and seed producing ecotypes were selected and planted in $1m{\times}1m$ maintenance plots. Seed yield ranged from 0.1 to $32.2g\;m^{-2}$. Highest yielding line was a medium leaf type zoysiagrass of Z6011 with $32.2g\;m^{-2}$. Most collected lines showed seed germination rates of below 50%. However, Z2095 showed highest germination rate of 78%. Considering germination rate and seed yield, collected lines of Z6011, Z 6015, Z1075, ZN1008, and Z1084, which were mostly medium leaf type and Z. japonica types, showed reasonably high potential to be used as breeding lines for high yield seed varieties of zoysiagrass.

• Weed & Turfgrass Science
• /
• v.2 no.3
• /
• pp.267-273
• /
• 2013

In this study, 241 zoysiagrasses (Zoysia spp.) accessions naturally growing in South Korea were collected in order to investigate the seed morphological traits. One of the goals of this study was to identify the morphological characteristics and variations of zoysiagrass seed. For the 241 zoysiagrasses accessions collected, a total of 41% (99 accessions) was interspecific hybrid zoysia, which showed various types of seed variations different from zoysiagrass species. Most of these hybrids zoysiagrasses were classified into Z. sinica type and Z. japonica type. Group I and II included Z. sinica type, this group showed 30.1 and 17.5 in number of seed per spike and 4.6 and 5.2 mm in seed length. Group III and IV included Z. japonica type, this group showed 59.2 and 45.8 in number of seed per spike and 3.3 and 3.4 mm in seed length. There is a need for additional research on growth characteristics and the molecular level for the interspecific hybridization which confirmed that genetic variation from level of gene flow between the species.

• Korean Journal of Soil Science and Fertilizer
• /
• v.35 no.2
• /
• pp.93-104
• /
• 2002

A field survey and experiment was conducted from 1996 to 1998 to develop rational technology for turfgrass vegetation of runway side of Incheon International Airport on the reclaimed tidal land in Young-Jong Island. Backfill of the experimental site was finished on August 1995. The experimental site was 8 ha located in the middle of the construction place for the main parking lot in front of the terminal building construction. The experimental field was drained by main open ditch, and divided three main plots, no subsurface tile drain, subsurface tile drain spacing with 22.5m, and with 45 m, respectively. The 17 sub plots were designed to test the effect of soil covering with red earth loam by 5 cm and 20 cm depth, application of chemical compound fertilizers and livestock manures, dressing of artifical soils and hydrophylic soil conditioners. The tested turfgrasses were three transplanting indigenous turfgrasses, Zoysia koreana, Zoysia sinica and Zoysia japonica, and two hydroseeding mixed exotic turgrasses, cool type I(tall fescue 30%, kentucky blue grass 40%, perenial ryegrass 30%), and cool type II(tall fescue 40%, perenial ryegrass 20%, fine fescue 20%, alkaligrass 20%). The soil backfilled with dredged seasand was sand textured with high salt concentration and low fertility. The soil showed high pH, low organic matter and low available phophate contents. The percolation rate was fast with high hydraulic conductivity. Desalinization was fast after installation of the main open drainage system. No subsurface tile drainage effect was found showing little difference in turfgrass growth. The covering and visual growth of turfgrasses were the best in the 20-cm soil covering with compound fertilizer treatment. The covering and visual growth of turfgrasses were satisfactory in the 5 cm soil covering with compound fertilizer treatment and with livestock manure treatments. The hydrophillic soil conditioner treatments were effective but expensive at present. The coverage and visual quality of turfgrasses were good for Zoysia koreana and Zoysia japonica. The coverages of turfgrasses by the hydroseeding with the mixed exotic turfgrasses were less than transplanting of native turfgrasses. In conclusion, for the runway side vegetation purposes, the subsurface tile drainage might not necessary as main open ditch drainage be sufficient due to fast percolation rate of the backfilled dredged seasand. The 5 cm soil covering with red earth might be sufficient for the runway side, but the 20 cm soil covering might be necessary for the runway side where high density of turfgrass coverage was necessary to protect from the airplance air blow.