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Control of Yam-Putrefactive Psychrotrophic Bacterium Using Clove Oil and Preparation of Functional Fresh-Cut (클로버 오일을 이용한 생마 저온부패균의 제어 및 기능성 생마 신선편이의 제조)

  • Ryu, Hee-Young;Park, Sang-Jo;Lee, Bong-Ho;Sohn, Ho-Yong
    • Microbiology and Biotechnology Letters
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    • v.35 no.1
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    • pp.66-72
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    • 2007
  • Yam has been recognized as healthy food due to its various biological activities, such as anti-obesity, antimicrobial, anticancer and immune-stimulation activities. In this study, antibacterial activities of 800 more natural plant extracts against yam-putrefactive psychrotrophic bacterium, Pseudomonas rhodesiae YAM-12, were evaluated to select a natural preservative, which is useful to long-term storage of yam and fresh-cut production. Finally, the clove oil was selected, and applied for the production of yam fresh-cut. The 1% of clove oil treated fresh-cut showed minor color changes during 31 days storage at $4^{\circ}C$ and the microbial growth was not detected. When the artificially contaminated fresh cut by dipping with P. rhodesiae YAM-12 suspension for 3 min was treated 1% clove oil, the microbial growth was identified to $10^4$ CFU/g from $10^3$ CFU/g with minor color changes. Whereas, treatment of sterilized water, or 100 ppm NaOCl into artificially contaminated fresh cut showed severe putrefaction $(10^8\;CFU/g)$ and color changes. Considering the previous reports that clove oil has antimicrobial, antioxidation, and antithrombosis activity, the use of clove oil into the yam fresh cut will provide market safety and consumer acceptability by prevention of microbial putrefaction and its biological activity.

Anti-oxidant Property and Tyrosinase Inhibition Activity of Various Extracts from plants in Compositae plants (국화류 추출물의 항산화 및 멜라닌 생성 억제 활성)

  • Kang, Jeong-Ran;Lee, Mi-Kyung;Kang, Sang-Mo
    • Applied Biological Chemistry
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    • v.51 no.4
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    • pp.321-328
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    • 2008
  • The research was conducted to identify the antimicrobial effect, anti-oxidative effect and tyrosinase inhibitory effect of MeOH 80% extract and n-hexane, chloroform, ethyl acetate, n-butanol and water fractions from the extract of six kinds of compositae plants, which are naturally grown across the nation. In the antimicrobial effect, the extract and chloroform fraction of Arctium lappa and hexane/ethyl acetate fractions of Taraxacum platycarpum exhibited significant inhibition. In case of antioxidant effect, the extract of Artemisia capillaries showed the highest effect and ethyl acetate/butanol fractions of all plants showed about 90%, which fractions were more polar than the fractions that showed antimicrobial effect. In case of tyrosinase activity, only the MeOH 80% of Arctium lappa among the extracts showed a potent inhibition, and butanol fraction of Chrysanthemum indicum, as well as ethyl acetate/water fractions of Artemisia capillaries showed 48, 38, and 37% respectively, which were higher than control group (arbutin). These active fractions in tyrosinase inhibition also were higher polarity than those that showed antimicrobial effect. In MeOH 80% extracts, only Arctium lappa was found to have antimicrobial, anti-oxidant and tyrosinase inhibitory activity, however there was no fraction to show effects commonly in the three assay system.

Studies on Rehabilitation Progress of Vegetation on Landslide Scars - In Cases of Yongin-gun·Hwaseong-gun at Areas - (산사태지(山沙汰地)의 식생(植生) 회복과정(回復過程)에 관(關)한 기초적(基礎的) 연구(硏究) - 용인(龍仁)·화성군지역(華城郡地域)을 대상(對象)으로 -)

  • Woo, Bo-Myeong;Jeon, Gi-Seong;Choi, Hyeong-Tae;Jeong, Do-Hyun
    • Journal of Korean Society of Forest Science
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    • v.84 no.1
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    • pp.31-40
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    • 1995
  • The purpose of this study was to analyze the process of natural plant establishment and revegetation on the slopes of landslide scars, both untreated(l4 plots) and treated plots (14 plots) with rehabilitation measures in 1993 and 1994 at Yongin Hwaseong-gun region. While total of 61 species were identified at surveyed sites. in the region, the pioneer species on landslide scars were Lespedeza cyrtobotrya, Arundinella hirta, Artemisia princeps, Pteridium acquilinum, Erigeron canadensis. Rubus crataegifolius, Stephanandra incisa etc. The species diversity (H') and evenness(E') of the untreated scars were greater than those of the treated scars. Similarity indices in the untreated scars were higher than those in the treated scars, and succession indices showed similar value in both areas. As a conclusion, this study shows that the untreated scars were more influenced by the around-vegetation, and the native vegetations(plants) like Lespedeza cyrtobotrya, Arundinella hirta. Miscanthus sinensis, Artemisia princeps, Pteridium acquilinum, Erigeron canadensis. Rubus crataegifolius, Stephanandra incisa ete. could be more effectively used as plant vegetation for rehabilitation of landslide scars.

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Reconsideration of Rhus (Anacardiaceae) with Respect to Fruit, Seed and Inflorescence (한국산(韓國産) 옻나무속(屬) 과실(果實)과 종자(種子) 및 화서(花序)의 분류학적(分類學的) 재고(再考))

  • Chun, Jae Min;Kim, Sam Shik
    • Journal of Korean Society of Forest Science
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    • v.86 no.3
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    • pp.288-300
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    • 1997
  • Morphology and anatomy of the fruit and seed of eight species of Rhus (Anacardiaceae) were investigated. The inflorescence of R. typhina was an apical and erect thysus, that of R. javanica was panicle. while that of the others were an axillary and pendulous panicle. Anatomical investigation of the pericarp showed that exocarp and mesocarp in R. javanica and R. typhina were integrated, but its in the others were disintegrated and uniform. In all these species, endocarp consisted of distinctly three lignified cell layers (outer, middle and inner endocarp) and crystal layers. The surface sculpturing of mature seed was variable and also contributed to understanding of species delimitation and relationships within the genus. Categorization by cluster analysis with 27 characters obtained from morphological and anatomical features of the fruit and seed resulted in two groups : R. trichocarpa - R. javanica group vs. R. ambigua - R. verniciflua - R. sylvestris - R. succedanea group. Taxonomic significance for the genus by these characters was supported by results of principal component analysis. The keys for the genus Rhus were provided using morphological and anatomical characters of the inflorescence, and fruit and seed.

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Comparison of Physicochemical Properties between Extracts and Soaked Liquor of Lespedeza cuneata G.Don (비수리(Lespedeza cuneata G.Don) 추출물과 담금주의 이화학적 특성비교)

  • Jeong, Kap-Seop
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.12
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    • pp.705-711
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    • 2017
  • Lespedeza cuneata (LC) is a herbaceous plant that grows throughout Korea. To investigate the physicochemical properties and the antioxidant activities of LC extracts and LC-soaked liquor (s. liquor), extraction with distilled water (DW) and ethanol was conducted and the extracts were compared with s. liquor. A total of 22 free amino acids from LC extract were detected, with the main ones being phosphoethanolamine 20.36>L-proline 18.02>ammonia 14.48>L-aspartic acid 12.96>${\gamma}$-amino-n-butyric acid 10.67%. The total flavonoid contents (TFC) and total phenolics contents (TPC) were in the order of s. liquor>DW>ethanol extract. The electron donating ability based on DPPH radical scavenging ability was highest for s.liquor, which equivalents 81.4% ascorbic acid in the order of s. liquor>ethanol>DW extract. The orders of ferric reducing antioxidant power and ABTS radical scavenging ability were proportional to the TFC and TPC of extracts, and in the order of s. liquor>ethanol>DW extract. The nitrite scavenging ability of s. liquor was highest among the three extracts (96.6% at pH 1.2), which was 2.6 times greater than that of DW extract.

Antioxidant Activity and Inhibitory Effect of Aster scaber Thunb. Extract on Adipocyte Differentiation in 3T3-L1 Cells (참취(Aster scaber Thunb.) 추출물의 항산화 효과와 3T3-L1 지방전구세포에서의 지방분화 억제 효과)

  • Choi, Jun-Hyeok;Park, Yun-Hee;Lee, In-Seon;Lee, Sam-Pin;Yu, Mi-Hee
    • Korean Journal of Food Science and Technology
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    • v.45 no.3
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    • pp.356-363
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    • 2013
  • Clinical and preclinical trials of involving drugs with anti-obesity effects have focused on screening for herbal medicines suspected to have anti-obesity activities. In this study, an extract of Aster scaver Thunb., which was prepared in 80% methanol (ASE), was assessed for its total phenol content, total flavonoid content, antioxidant activity ability to scavenge the ${\alpha}-{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl, 2,2'-azino-bis-[3-ethylbenzthiazoline]-6-sulfonic acid radical, and anti-adipogenic effects. The anti-adipogenic effect of ASE on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by assaying the suppression of adipocyte differentiation and lipid accumulation by using western blot analysis and the Oil Red-O assay, respectively. The staining results showed that ASE significantly inhibited 3T3-L1. Western blot analysis results showed that ASE decreased the levels of peroxisome proliferator-activated receptor-${\gamma}$, CCAAT/enhancer-binding protein ${\alpha}$, and sterol regulatory element-binding protein 1c. These results demonstrate that ASE directly inhibits the differentiation of preadipocytes, and might be an important adjunct in the therapeutic efforts to reduce adipogenesis.

Antifungal Activity on the Water Extracts of Five Fagaceae Plants (참나무과 수목 5종 수용성 추출물의 항균활성)

  • Moon, Sang-Ho;Song, Chang-Khil;Kim, Tae-Keun;Oh, Dong-Eun;Kim, Hyoun-Chol
    • Korean Journal of Organic Agriculture
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    • v.25 no.2
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    • pp.295-310
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    • 2017
  • This study investigated the growth of five phytopathogenic fungi including Colletotrichum gloeosporioides, Diaporthe citri, Phytophthora capsici and others according to different concentrations of water extract in order to provide reference data for developing environment-friendly agricultural materials using five native Fagaceae species including Quercus acuta, Quercus salicina, Quercus glauca, Quercus gilva and Castanopsis cuspidata var. sieboldii. As the concentration of aqueous extracts of Fagaceae increased according to donor plants, the mycelial growth of phytopathogens showed a decreasing tendency. Differences were found in the degree of inhibition according to types of donor plants and pathogenic fungi. Diaporthe citri, Phytophthora capsici, Pythium graminicola on the water extract of Castanopsis cuspidata var. sieboldii inhibited mycelial growth by 84% in 25% of the treatment group and by 87% in more than half of the treatment group. The water extract of Quercus acuta was found to have no inhibitory effect against the mycelial growth of Diaporthe citri. The aqueous extracts of Quercus salicina, Quercus glauca and Quercus gilva insignificantly inhibited mycelial growth by approximately 15%. The total phenolic content of receptor plants exhibiting antifungal activity was highest in Castanopsis cuspidata var. sieboldii with a content of 22.32 mg/g phenols, followed by Quercus salicina with 8.32 mg/g, Quercus glauca with 6.83 mg/g, Quercus gilva with 5.95 mg/g, and Quercus acuta with 5.24 mg/g. The aqueous extracts of Castanopsis cuspidata var. sieboldii among the five Five Fagaceae Plants of were the most effective antifungal activity.

Antioxidative Activities and Whitening Effects of Ethyl Acetate Fractions from The Immature Seeds of Abeliophyllum distichum (미선나무 미성숙 종자의 항산화 및 미백 활성)

  • Jang, Tae Won;Park, Jae Ho
    • Journal of Life Science
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    • v.27 no.5
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    • pp.536-544
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    • 2017
  • Abeliophyllum distichum Nakai is deciduous shrubs of flowering plant in Oleaceae. It is important plant resources and consists of one species in the world. Also the endemic plant of A. distichum has been protected and designed endangered plant in Korea. For this reason, study on the immature seeds of A. distichum (ADS) hasn't progressed. In the present study, we evaluated the antioxidant activity and inhibitory effects on proteins and mRNA levels were related in the whitening effect in B16F10 cells. ADS was effective for reaction oxygen species (ROS). ROS causes various diseases such as aging, inflammation, cancer, and etc. Antioxidant properties were evaluated DPPH, ABTS radical scavenging activity and Reducing power. Plants were known that contained phenolic compounds were related in antioxidant activity. Phenolic compounds were phytochemicals commonly named natural polyphenols. These are secondary metabolites of plants involved in the defense against different types of stresses. In results, ADS suppressed the expression and transcription of Tyrosinase, TRP-1, TRP-2, and Microphthalmia-associated transcription factor (MITF). Tyrosinase, tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 1 (TRP-2) are known to play an important role in melanin biosynthesis. MITF regulated the expression and transcription of Tyrosinase, TRP-1, and TRP-2. In conclusion, ADS was effective in both antioxidant activities and whitening effects. Also, they were associated with the content of phenolic compounds. We suggested that ADS can be use antioxidants and skin-whitening functional cosmetics material derived from natural plant resources.

Vehicle Ramming Terror Attacks and Physical Barriers as a Counterterrorism Policy (차량돌진테러와 물리적 방어물에 관한 연구)

  • Yun, Minwoo;Kim, Eunyoung
    • Korean Security Journal
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    • no.55
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    • pp.9-29
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    • 2018
  • Recently, it has been frequently reported there were rapid increase of vehicle ramming attacks in the Western countries, such as Europe, United States of America, Australia, and Canada. Vehicle ramming attacks happened in Western countries specifically targeted civilians and maliciously intented to attack as many victims as possible. and resulted in significant number of casualties and wounds. Experts in terrorism analyze the increase of terrors using vehicle is largely due to the change of terror strategy of Islamic extremest groups like ISIS which encouraging would-be terrorist to use vehicles as an effective killing weapons. Accordingly, The most of countries experienced vehicle terrorist attacks began to build physical barriers including ballards, fences, and obstacles on the main shopping streets, transportation facilities, and famous crowded places and buildings in order to prevent mass killing by terrorists' vehicle ramming attack. Contrary to such swift respond to be prepared attacks using vehicle as a weapon Western countries, there are still lack of interests in preparing this type of terrorist attacks among domestic policy makers and scholars. To fulfill the research gap, this study aimed to investigate important issues regarding physical barriers in South Korea. The contributions, implications of this study and suggestions for policy implications of this study findings were discussed in results and discuss.

Bioactive Compounds and Antioxidant Activity of Jeju Camellia Mistletoe (Korthalsella japonica Engl.) (제주 동백나무 겨우살이의 용매별 기능성 성분 및 항산화 작용)

  • Kang, Da Hee;Park, Eun Mi;Kim, Ji Hye;Yang, Jung Woo;Kim, Jung Hyun;Kim, Min Young
    • Journal of Life Science
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    • v.26 no.9
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    • pp.1074-1081
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    • 2016
  • Mistletoes are hemi-parasitic plant growing on different host tree and shrubs. They are traditionally used in folkloric medicine for the treatment of diarrhea, cough, diabetes, hypertension, cancer and skin infection. The purpose of this study was to determine the contents of phenolics and antioxidant activity of 70% ethanol, 100% methanol and hot water extracts of Jeju camellia mistletoe (Korthalsella japonica Engl.). Ethanol was most effective in extracting total phenols (7,427 mg gallic acid equivalent (GAE)/100 g) and flavonoid (1,777 mg rutin equivalent (RE)/100 g). The free radical scavenging activity, 1,1-diphenyl-2-picryl hydrazyl (DPPH) (EC50 = 7.8 mg/ml) and hydrogen peroxide (H2O2) (EC50 = 1.4 mg/ml), and the capacity for chelating metal ions (EC50 = 8.0 mg/ml) and reducing power (EC50 = 14.9 mg/ml) of the samples also higher in ethanolic extracts. The strong correlation (r2 = −0.996~−0.881) between antioxidant capacities and the phenolic contents implied that phenolic compounds are a major contributor to the antioxidant activity of the ethanolic extracts of Jeju camellia mistletoe. As conclusions, Jeju camellia mistletoe contains bioactive substances with a potential for reducing the physiological as well as oxidative stress and this could explain the suggested cancer preventive effect of these plants as well as their protective role on other major diseases.