• Title/Summary/Keyword: 일차성

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An Enzyme-Linked Immunosorbent Assay for Fumonisins in Corn without Cleanup Procedure (추출물의 희석에 의한 옥수수 중 Fumonisin의 효소면역측정법)

  • Shon, Dong-Hwa;Kim, Young-Mok
    • Korean Journal of Food Science and Technology
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    • v.28 no.5
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    • pp.953-958
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    • 1996
  • A simple and rapid ELISA (enzyme-linked immunosorbent assay) system for fumonisins, a group of potentent carcinogen, was developed. To produce anti-fumonisin B1 (FB1) antibodies, FB1 conjugated to keyhole lympet hemocyanin (KLH) and Freund's adjuvant were immunized into rabbits subcutaneously 3 times. From one of the antisera showing high titer and good competition with the toxin in ELISA, polyclonal antibodies were purified. The cross-reactivities of the antibodies against fumonisin $B_1,\;B_2\;and\;B_3$ were 100%, 69%, and 166%, respectively. When competitive direct ELISA established by use of the antibody was applied to the spike test of $FB_1$ onto uncontaminated corns, the assay recovery was unstable unless 75% methanol extracts of corn were diluted to 1/100 with buffer. In that condition the mean ELISA recovery of FB1 from corns spiked $1-30\;{\mu}g/g$ was 67% and stable (coefficient of variation (CV) of each recovery percentage, 3.4%). The results suggest that the ELISA system established in this study needs no cleanup procedure and therefore would be powerful to screen a large number of corn samples contaminated with fumonisins.

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White Ginseng Saponin Upregulated the Production of -TNFTNF-α, IL-1β and NO in Primary Cultures of Mixed Glial Cells (고려인삼에 의한 신경면역 및 염증반응 조절: 백삼사포닌에 의한 교세포에서의 TNF-α, IL-1β 및 NO 생성 증가)

  • 성정훈;최동희;김동훈;전보권;최상현
    • Journal of Ginseng Research
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    • v.28 no.2
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    • pp.120-126
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    • 2004
  • Glial cells such as astrocytes and microglial cells are the main source of proinflammatory cytokines and nitric oxide(NO) in the central nervous system, which exert neuroimmune and inflammatory functions and other various neurobiologic effects. Though Panax ginseng C.A. Meyer has been known to strengthen the body's defence mechanisms and also to maintain the homeostasis in the central nervous system, the effects of Panax ginseng on the production of immune and inflammatory mediators have not been studied well in the brain. Therefore, this study was designed to study the effects of ginseng saponins on the production of proinflammatory cytokines and NO in the primary cultures of mixed glial cells. White ginseng saponin, 200-500 $\mu$g/ml, showed significant cytotoxicity after 72 hrs and increased TNF-$\alpha$, IL-$\beta$, and NO production. Lower doses of 50-100 $\mu\textrm{g}$/ml showed little cytotoxicity until 72 hrs and also increased the production of TNF-$\alpha$, IL-1$\beta$, and NO. Triple immune staining showed that white ginseng saponin, 200$\mu\textrm{g}$/ml for 72 ks, induced stellation of astrocytes and iNOS expression exclusively in microglial cells. Taken together, the white ginseng saponin increased the production of proinflammatory cytokines such as TNF-$\alpha$ and IL-1$\beta$, and induced iNOS expression and NO production in mixed glial cell cultures, which may be ascribed to the enhancement of central immune responses and the regulation of inflammatory reactions by Panax ginseng.

Effect of Microwave Treatment on the Physicochemical and Microbiological Characteristics of Beef Loin during Storage at 4℃ (마이크로파 처리가 4℃ 저장 중 우육의 이화학적 및 미생물학적 특성에 미치는 영향)

  • Kang, Ho Jin;Lee, Hyun-Yu;Park, Jong-Dae;Kum, Jun-Seok
    • Korean Journal of Food Science and Technology
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    • v.45 no.2
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    • pp.161-166
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    • 2013
  • This study was carried out to investigate the physicochemical and microbiological characteristics of beef loin following microwave treatment at $4^{\circ}C$. Two types of microwave treatment were applied. i.e., continued microwave treatment (CW) and holding microwave treatment (HW). The L value increased, while a and b values were not significantly different among the samples as the storage time and microwave dose increased. The initial pH and after 3 days ranged from 5.51-5.74 and 6.32-6.51, respectively (p<0.05). The thiobarbituric acid value of all beef loins increased with increasing storage period and decreased with increasing microwave dose. The volatile basic nitrogen (VBN) content of the control was higher than that of the other samples and the VBN content decreased with increasing microwave dose. The total plate count of beef loins decreased with increasing microwave dose. This result indicated that T2 (100 W, HW) is most effective treatment with regard to the safety of beef loins without decreasing the physicochemical and microbiological characteristics.

An Analysis on Patients Trend and Income of Primary Care Clinic (일차 진료의원의 진료수입의 형평성 분석연구)

  • Lim, Sun Mi;Im, Geum Ja;Park, Kwan Jun;Park, Yoon Hyung
    • Health Policy and Management
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    • v.24 no.1
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    • pp.92-99
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    • 2014
  • Background: Korea's primary care clinics are seeking increase in consultation fees by expanding supply within the frame of the health insurance system, but inequality of physician income between regions and individuals is exacerbating. The purpose of this study lies in analyzing the distribution of patients of primary care clinics, their specialized field, and the degree of inequality between medical fee income according to region. Data was acquired from the Health Insurance Review and Assessment Service on charged bills made by clinic-size medical institutions from 2008 to 2011. Methods: By comparing the outpatient number per clinic according to the clinic's specialized field, results showed that ophthalmology, otolaryngology, dermatology, orthopedics, and internal medicine showed high numbers whereas plastic surgery, neuropsychiatry, cardiothoracic surgery had fewer outpatients. The number of outpatients for clinic according to region showed Chuncheonnam-do, Jeju-do, Gangwon-do, Chungcheongbuk-do, Ulsan to have higher numbers of outpatients. For those four years, clinics in the Seoul area had a rather lower number. Results: As a result of comparing the decile hierarchy distribution ratio between specialized fields according to primary care clinics income from National Health Insurance, the inequality degree showed that obstetrics and gynecology and general medicine were each 0.130, 0.280 for the decile distribution ratio, which was the highest degree of inequality within the specialized field. Their Gini coefficient were also relatively high at 0.691, 0.528 respectively. On the other hand, the decile distribution ratio for otolaryngology and orthopedics were 0.510, 0.468, respectively, while their Gini coefficient each at 0.318, 0.314 makes their inequality degree relatively lower than other fields. Conclusion: This study is limited in that the data used was the health insurance charges submitted by clinics, which does not provide total information of the doctors' income. However, because most clinics are largely dependant on their income to come from health insurance reimbursements. Therefore, the results of this study can be used effectively. In the future, research that includes data on non-covered service income should be conducted to closely examine policy plans with a new medical fee policy which can resolve the medical fee income inequality issue between clinics as well as revitalize primary medical care.

Identification of the Oligotrophic Bacteria Strain 7F Biocontrolling Phytophthora Blight Disease of Red-pepper (고추 역병 방제를 위한 저영양 길항세균 7F 균주의 동정)

  • Kim, Dong-Gwan;Yeo, Yun-Soo;Kwon, Soon-Wo;Jang, Kil-Su;Lee, Chang-Muk;Lee, Mi-Hye;Kim, Soo-Jin;Koo, Bon-Sung;Yoon, Sang-Hong
    • Research in Plant Disease
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    • v.16 no.1
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    • pp.41-47
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    • 2010
  • A total of 10,753 oligotrophic bacteria were isolated from the cultivated soils of red-pepper infected by Phytophthora blight disease in various regions of Korea (Chungju, Anmyon, Taean, Andong, Eumsung and Goesan). Seven bacteria isolates among these collected resources were selected by the first screening of in vitro antagonistic assay against major several plant pathogenic fungi including Phytophthora capsici. Finally, strain 7F was selected by pot assay for a possible biological control agent against Phytophthora blight disease of pepper seedling in the greenhouse. Strain 7F was identified as Bacillus subtilis on the basis of its 16S rDNA sequence analysis and as standardized biochemical characteristics assay kits such as API20 NE. In the experiment of P. capsici zoospore infected red-pepper on the pot test, infection rate of red-pepper with nonetreatment to Phytophthora blight disease was 87%, while the rate was only 6% in the pot treated with strain 7F. This result indicated that the Bacillus subtilis strain 7F will be useful as a potential biocontrol agent for Phytophthora blight disease of red-pepper.

Seasonal Variation in Abundance and Species Composition of Surf Zone Fish Assemblage at Taecheon Sang Beach, Korea (大川海濱 碎波帶 魚類群集의 季節變化)

  • 신민철;이태원
    • 한국해양학회지
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    • v.25 no.3
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    • pp.135-144
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    • 1990
  • Fish assemblage occupying the surf zone habitat of Taecheon sand beach in Cheonsu Bay was analyzed using monthly samples collected by a beach seine from September 1984 through August 1985. During each sampling period five hauls were completed, one at each 2-hour interval during the spring tide. Of 35 species identified, Favonigobius gymnauchen, Sillago japonica, Konosirus punctatus, Sardinella zunasi and chaenogobius mororana accounted for 79% of the individuals collected, and F. gymnauchen, s. japonica, K. punctatus, S. zunasi and Kareius bicoloratus constituted 67% of the biomass obtained. Overall abundance in terms of numbers and in biomass were 0.13 individuals/m$^2$ and 0.33g/m$^2$, respectively. A larger number of individuals and greater biomass was observed during the warmer months showing significant correlation with water temperature. High abundance of June was related to the occurrence of adult F. gymnauchen. A large number of individuals and great biomass was collected in August and September, a period of high abundance in juveniles of the seasonal species such as S. japonica, K. punctatus and S. zunasi. Temperature and habitat occupation according to life history of the dominant species might be the primary factors of the marked seasonality of the surf zone fish populations in the study area.

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Measuring the quality of research papers across countries using Relatively Rank-normalized Impact Factor ($R^2nIF$) (상대적순위보정지수($R^2nIF$)를 활용한 주요국의 SCI 논문 질적 수준 비교분석)

  • Oh, Donghun;Kim, Youngjun;Kim, Yongjeong
    • Journal of Technology Innovation
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    • v.21 no.1
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    • pp.85-108
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    • 2013
  • This paper introduces a new qualitative measurement indicator, Relatively Rank-normalized Impact Factor ($R^2nIF$) that can overcome drawbacks of the existing research performance measures. With the help of this innovative indicator, this study analyzes and compares the quality of research papers across countries and National Science Indicators (NSI) standard academic fields. The development of an improved bibliometric indicator for evaluating the quality of research papers and disentangling the "international" dimension of research performance will be of interest to academics and practitioners alike.

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A Comprehensive Groundwater Modeling using Multicomponent Multiphase Theory: 1. Development of a Multidimensional Finite Element Model (다중 다상이론을 이용한 통합적 지하수 모델링: 1. 다차원 유한요소 모형의 개발)

  • Joon Hyun Kim
    • Journal of Korea Soil Environment Society
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    • v.1 no.1
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    • pp.89-102
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    • 1996
  • An integrated model is presented to describe underground flow and mass transport, using a multicomponent multiphase approach. The comprehensive governing equation is derived considering mass and force balances of chemical species over four phases(water, oil, air, and soil) in a schematic elementary volume. Compact and systemati notations of relevant variables and equations are introduced to facilitate the inclusion of complex migration and transformation processes, and variable spatial dimensions. The resulting nonlinear system is solved by a multidimensional finite element code. The developed code with dynamic array allocation, is sufficiently flexible to work across a wide spectrum of computers, including an IBM ES 9000/900 vector facility, SP2 cluster machine, Unix workstations and PCs, for one-, two and three-dimensional problems. To reduce the computation time and storage requirements, the system equations are decoupled and solved using a banded global matrix solver, with the vector and parallel processing on the IBM 9000. To avoide the numerical oscillations of the nonlinear problems in the case of convective dominant transport, the techniques of upstream weighting, mass lumping, and elementary-wise parameter evaluation are applied. The instability and convergence criteria of the nonlinear problems are studied for the one-dimensional analogue of FEM and FDM. Modeling capacity is presented in the simulation of three dimensional composite multiphase TCE migration. Comprehesive simulation feature of the code is presented in a companion paper of this issue for the specific groundwater or flow and contamination problems.

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The Effects of Gender Cue and Antecedent Case on the Immediacy of Pronominal Resolution (대명사의 성별단서와 선행어 격이 참조해결의 즉각성에 미치는 효과)

  • JaehoLee
    • Korean Journal of Cognitive Science
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    • v.4 no.1
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    • pp.51-86
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    • 1993
  • The purpose of this study was to investigate on-line comprehension processing in pronoun resolution. The two important constraints investigated in this study were the gender cue of pronoun and the antecedent case. Using antecedent probe recognition task. Experiment 1 investigated the effects of gender cues and antecedent cases on probe recognition time. There were on signigicant effects of employed variable. This result suggest the possibilty of immediate antecedent assignment depending on the degree of syntactic constraints satisfaction. In Experiment 2. using antecedent probe recognition task. the primed activation level differences between antecedents and non-antecedents over time-course intervals from 0 to 250msec were measured. The effect of gender cues was obtained over 0-250msec time-course condition. This indicates that the gender cues can determine the assignment of proper antecedent for a pronoun. In Experiment 3, subect-case pronouns were used only:Unambiguous gender cues were given and the time-course intervals of 250 and 750msec were employed. A signigicant interaction effect of antecedent cases with probe conditions was obtained. All the results of this research suggest that gender cues are powerful constraints for pronoun resolution.

Effects of Temperature and Pressure on the Reaction of [Pt(dien)X]$^+$ with ${NO_2}^-$ Aqueous Solutions (수용액에서 [Pt(dien)X]$^+$${NO_2}^-$와의 치환반응에 미치는 온도 및 압력변화)

  • Sang Oh Oh;Sang Hyup Lee;Hwan Jin Yeo;Jong Wan Lim;Du Hwan Jo
    • Journal of the Korean Chemical Society
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    • v.33 no.4
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    • pp.371-378
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    • 1989
  • Kinetics of the substitution reaction of [pt[dien]X]$^+$(X = $Cl^-$, $Br^-$, $I^-$) with NO2- in aqueous solution were investigated by a spectrophotometric method at $20-35^{\circ}C$ and 1-1500 bars. The rates of these reactions were increased with raising temperature and pressure. The relative reactivities of replaceable ligands in [pt[dien]X]$^+$ follow the order ($Cl^-$ > $Br^-$ > $I^-$). Activation volumes (${\mid}{\Delta}V^{\neq}{\mid}$) were large negative values and decreased with raising temperature. First ($k_1$) and second($k_2$) order rate constants of these reactions were determined at $25^{\circ}C$ and both $k_1$ and $k_2$ were increased with raising pressure and activation volumes (${\Delta}{V_1}^{\neq}$, ${\Delta}{V_2}^{\neq}$) for the $k_1$ and $k_2$ path were large negative values respectively. The $k_1$ and $k_2$ of these reactions were determined at 1 bar and these values were increased with raising temperature. Activation parameters of $k_1$ and $k_2$ path were determined, therefore it can be inferred frp, these results that the substitution reactions of [pt[dien]X]$^+$ with ${NO_2}^-$ proceed through on associative (A) mechanism independent of a leaving groups and reaction paths.

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