• Journal of Life Science
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• v.21 no.2
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• pp.202-210
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• 2011

Adipocyte differentiation is an ordered multistep process requiring the sequential activation of several groups of adipogenic transcription factors, including CCAAT/enhancer-binding protein-$\alpha$ and peroxisome proliferator-activated receptor-$\gamma$, and coactivators. In previous reports, we identified that replication factor C 140 (RFC140) protein played a critical role in regulating adipocyte differentiation as a coactivator. Here, we show expressional regulation of RFC140 and small RFC subunit, RFC38, following characterization of gene promoter of RFC140 and RFC38. In addition, RFC140 increases PPAR$\gamma$-mediated gene activation, resulting from direct protein-protein interaction of RFC140 and PPAR$\gamma$. Taken together, these findings demonstrate that the regulated expression of RFC140 and RFC38 by specific adipocyte transcription factors is required for the adipocyte differentiation process.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.75-75
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• 2017

하천의 합류부는 서로 다른 지형학적 특성과 수리학적 특성을 가지는 두 개의 하천이 하나로 합쳐지는 구간으로 급격한 흐름의 변화 및 퇴적물의 유입과 수리학적 지형변화가 발생하는 구간이다. 이러한 합류부에서는 흐름구조와 물의 물리 화학적인 특성이 지속적으로 변화할 수 있고, 침식과 퇴적으로 인한 하상변동과 같은 하도 변화가 발생할 수 있다. 또한, 하천의 합류부는 두 지류가 만나 형성되는 지역으로 하천생태계에서 매우 중요한 역할을 하는 구간이다. 이러한 합류부 구간의 혼합 매커니즘을 이해하기 위해서는 지류의 다양한 유입조건에 따른 본류와의 수체혼합의 공간적인 패턴을 분석하는 것이 중요하다. 그러나, 대부분의 합류부 연구들은 실측에 기반한 공간적인 수체혼합의 패턴 분석의 어려움으로 인해 수리 및 수질 수치모형에 의존하여, 실측자료에 기반하여 지류의 유입에 따른 수체혼합을 공간적으로 분석하는 연구는 매우 제한적이었다. 따라서, 본 연구에서는 ADCP를 활용하여 합류부의 혼합 현상을 규명하는 인자로 유속과 수심 등 기본적인 수리학적 인자들뿐만 아니라 최근 활발하게 연구되고 있는 ADCP의 초음파반사율을 활용하여 상이한 농도의 유사가 혼합되는 양상을 측정하여 합류부의 혼합 특성을 공간적으로 분석하고자 하였다. 초음파반사율은 부유사와 관련되는 인자로 SonTek ADCP 이동식으로 측정된 SNR자료를 확산, 물에 의한 흡수를 고려하여 보정한 후 3차원 혼합거동의 공간적인 분포를 도출하였다. 그리고 초음파반사율을 활용한 방식을 검증하고 지류와 본류의 유입 전후 입도분포를 확인하기 위해 LISST 측정을 수행하였고, 드론 영상을 활용하여 유입 유사의 2차원 공간적인 분포를 확보하여 초음파반사율을 활용한 방법과 비교하였다. ADCP, LISST, 드론의 계측자료는 낙동강과 남강 합류부에서 측정되었고, ADCP로부터 제공되는 유속, 수심자료의 공간적인 분포를 분석하여 합류부의 수리특성을 분석할 수 있었고, 초음파반사율을 유사 혼합의 공간적 특성을 규명하는 인자로 활용하여 본류와 지류의 유입량에 따른 다양한 유사 혼합의 3차원 공간적인 패턴을 분석할 수 있었다.

• Journal of Life Science
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• v.33 no.1
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• pp.34-42
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• 2023

In a signal transduction network, from the perception of stress signals to stress-responsive gene ex- pression, binding of various transcription factors to cis-acting elements in stress-responsive promoters coordinate the adaptation of plants to abiotic stresses. Among the AP2/ERF transcription factor family genes, group VII ERF genes, such as RAP2.12, RAP2.2, RAP2.3, AtERF73/HRE1, and AtERF71/ HRE2, are known to be involved in the response to hypoxia stress in Arabidopsis. In this study, we dissected the HRE1 promoter to identify hypoxia-responsive region(s). The 1,000 bp upstream promoter region of HRE1 showed increased promoter activity in Arabidopsis protoplasts and transgenic plants under hypoxia conditions. Analysis of the promoter deletion series of HRE1, including 1,000 bp, 800 bp, 600 bp, 400 bp, 200 bp, 100 bp, and 50 bp upstream promoter regions, using firefly luciferase and GUS as reporter genes indicated that the -200 to -100 region of the HRE1 promoter is responsible for the transcriptional activation of HRE1 in response to hypoxia. In addition, we identified two putative hypoxia-responsive cis-acting elements, the ERF-binding site and DOF-binding site, in the -200 to -100 region of the HRE1 promoter, suggesting that the expression of HRE1 might be regulated via the ERF transcription factor(s) and/or DOF transcription factor(s). Collectively, our results suggest that HRE1 contains hypoxia-responsive cis-acting elements in the -200 to -100 region of its promoter.

• Economic and Environmental Geology
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• v.56 no.5
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• pp.533-545
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• 2023

In this study, an investigation was conducted on the features, events, and processes (FEP) that could impact the long-term safety of the natural barriers constituting high-level radioactive waste geological repositories. The FEP list was developed utilizing the IFEP list 3.0 provided by the Nuclear Energy Agency (NEA) as foundational data, supplemented by geological investigations and research findings from leading countries in this field. A total of 49 FEPs related to the performance of the natural barrier were identified. For each FEP, detailed definitions, classifications, impacts on long-term safety, significance in domestic conditions, and feasibility of quantification were provided. Moreover, based on the compiled FEP list, three scenarios that could affect the long-term safety of the disposal facility were developed. Geological factors affecting the performance of the natural barrier in each scenario were selected and their relationships were visualized. The constructed FEP list and the visualization of interrelated factors in various scenarios are anticipated to provide essential information for selecting and organizing factors that must be considered in the development of mathematical models for quantitatively evaluating the long-term safety of deep geological repositories. In addition, these findings could be effectively utilized in establishing criteria related to the key performance of natural barriers for the confirmation of repository sites.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.296-296
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• 2019

전 세계적으로 기후변화로 인한 국지성 집중호우 및 태풍으로 인한 피해가 지속적으로 발생하고 있으며, 그에 따른 2차 피해인 산사태 및 토석류 피해 또한 증가하고 있는 추세이다. 최근 국내의 산사태 및 토석류에 대한 선행연구는 지속적으로 수행되고 있으나, 산사태 및 토석류 위험성이 높은 구간, 즉, 발생기작을 판단할 수 있도록 지표화 해놓은 것이며, 현재 피해예측지도 및 피해 하류부의 시설물을 고려한 연구는 미비한 실정이다. 따라서 본 연구에서는 강우-유출모형인 S-RAT모형 및 토석류 수치해석 프로그램 RAMMS 모형을 이용하여 산사태 및 토석류 피해를 극대화 시키는 인자인 토석유동심(H), 토석유속(V)을 이용하여 토석류피해예측지도를 작성하였으며, 피해 하류부의 시설물을 건물 유형별 시설물의 중요도로 구분하였다. 또한 작성된 피해예측지도 및 시설물 중요도를 중첩하여 위험성 지도를 제시하였다.

• Journal of Animal Science and Technology
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• v.50 no.5
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• pp.633-640
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• 2008

With a double mismatch primer set designed for amplifying the modified DNA sequence fragments, bovine melanocortin-1 receptor(MC1R) gene encoded in Extension locus which plays a critical role in coat color development was analyzed using polymerase chain reaction mediated restriction fragment length polymorphism(PCR-RFLP). Amplified PCR fragments were successfully discriminated with combining the MspI- and AluI-RFLP into three major alleles(ED, E+, and e), directly related to bovine coat color phenotypes. The genotyping results showed that Jeju black cattle contained three MC1R alleles, but yellowish-red colored Hanwoo and bridle colored Korean Brindle cattle did not contained the dominant black allele ED. However, two dominant black-colored cattle breeds, Holstein and Angus, contained the ED allele over 96% in frequency. Hanwoo×Holstein F1 and Hanwoo×Angus F1 crossbred calves showed ED/e MC1R genotypes, and uniformly black coat color. the results suggested that this MC1R genotyping method be useful in allele discrimination for bovine MC1R gene which used for breed classification and characterization, as one of the important genetic markers, using combination of MspI- and AluI-RFLP for modified PCR product amplified with a newly designed double mismatch primer set.

• Tuberculosis and Respiratory Diseases
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• v.62 no.6
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• pp.516-522
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• 2007

Background: Postoperative pulmonary complications are important problems that prolong the length of the hospital stay and increase patient mortality. However, our knowledge of the postoperative factors that increase the risk of these complications is less than complete. Methods: We conducted a prospective study to determine the risk factors for postoperative pulmonary complications. A total of 199 patients were referred to the Pulmonology Department for preoperative pulmonary evaluation. We reviewed the perioperative variables according to the National Surgical Quality Improvement Program (NSQIP). Postoperatively, we collected data on the occurrence of postoperative pulmonary complications and we evaluated the relationship between the perioperative variables and outcomes. Results: Twenty two patients (11%) had pulmonary complications; 6 had respiratory failure, 4 had pneumonia, 13 had pleural effusion and, 2 had atelectasis. Five variables were statistically significantly associated with pulmonary complications on the bivariate analyses. Multiple logistic regression analyses revealed that three of these variables were independently associated with an increased risk of pulmonary complications; a high cardiac risk index (OR 16.5, p=0.002), emergency surgery (OR 10.3, p=0.017), and thoracic/abdominal surgery (OR 3.8, p=0.047). Conclusion: The risk factors for postoperative pulmonary complications are a high cardiac risk index, emergency surgery and thoracic/abdominal surgery.

• The Journal of Engineering Geology
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• v.27 no.2
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• pp.165-172
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• 2017

This research aims to define influence factors to perform an optimized section design and evaluate tunnel collapse risk during construction using Delphi technique. A total of five upper classification systems were constructed through literature review, pervious research analysis, and brainstorming of expert group for establishing influence factors. The $1^{st}$, $2^{nd}$, and $3^{rd}$ Delphi survey process was proceeded by panel group which is consisted 21 experts to prevent errors and bias in the expert judgement process. In Delphi $1^{st}$ survey, a total of 22 influence factors candidates were derived through open-ended questionnaire. In Delphi $2^{nd}$ survey, questionnaire was proceeded based on 7-point Likert scale method. In order to verify the validity, CVR (Content Validity Ration) analysis was performed to exclude inappropriate candidates. In the $3^{rd}$ survey, verification of influence factors was proceeded once more with the result of $2^{nd}$ survey, and lastly, a total of 14 influence factors was derived by CVR and COV (Content Validity Ration) analysis for response of experts.

• Journal of Chest Surgery
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• v.34 no.12
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• pp.937-943
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• 2001

Background: The aims of this study are to determine the predicting factors related with cardiovascular injuries in sternal fractures and to suggest the indication of emergency echocardiography. Material and Method: A retrospective review of the Ewha Womans University Mok-Dong Hospital trauma registry revealed 40 patients, that visited the hospital with a sternal fracture a over 5-year period. We analyzed 4 factors as predicting factors; 1) presence of restraint, 2) presence of associated injuries, 3) presence of a past medical history involving cardiovascular system, and 4) Revised Trauma Score(RTS). We, also, assessed the utility of conventional study methods involving cardiovascular injuries, such as ECG, chest X-ray, and enzyme level. Based on the methods, we inferred an indication for emergency echocardiography in sternal fractures. Result: Statistically significant predicting factors were the presence of a past medical history involving cardiovascular system and abnormal RTS on admission. Usage of emergency echocardiography depended upon the predicting factors and the results from conventional evaluations. We can now suggest the indications of emergency echocardiography in sternal fractures as 1) if more than two studies reveal abnormality without any significant predicting factors, and 2) if more than one study reveals abnormality with any significant predicting factors. Conclusion: The past medical history involving cardiovascular system and initial vital signs imply the presence of cardiovascular injuries in sternal fractures. And if needed, emergency echocardiography should be performed.

• Journal of Korean Society of Forest Science
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• v.109 no.3
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• pp.350-360
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• 2020

The purpose of this study was to identify and prioritize the key indicators and drivers of forest thinning. The research for this study was designed in two-phases: 1) sequential, exploratory, mixed methods research that was initiated with a qualitative phase (Delphi technique), and 2) the quantitative phase (Analytic Hierarchy Process technique). Results indicated that management and planning were the most important factors in the first level of criteria among the "management and planning," "directing and monitoring," "supervision," and "quality of thinning work." On the sub-criteria level, "the quality of forest management planning" was indicated as the most important factor among the ten sub-criteria. Our results have shown that the developed forest thinning evaluation factors were a well-represented characteristic for a variety of forest thinning work in Korea.