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http://dx.doi.org/10.5352/JLS.2011.21.2.202

Expressional Regulation of Replication Factor C in Adipocyte Differentiation  

Cho, Hyun-Kook (Department of Molecular Biology, Pusan National University)
Kim, Hye-Young (Department of Molecular Biology, Pusan National University)
Yu, Hyun-Jeong (Department of Molecular Biology, Pusan National University)
Cheong, Jae-Hun (Department of Molecular Biology, Pusan National University)
Publication Information
Journal of Life Science / v.21, no.2, 2011 , pp. 202-210 More about this Journal
Abstract
Adipocyte differentiation is an ordered multistep process requiring the sequential activation of several groups of adipogenic transcription factors, including CCAAT/enhancer-binding protein-$\alpha$ and peroxisome proliferator-activated receptor-$\gamma$, and coactivators. In previous reports, we identified that replication factor C 140 (RFC140) protein played a critical role in regulating adipocyte differentiation as a coactivator. Here, we show expressional regulation of RFC140 and small RFC subunit, RFC38, following characterization of gene promoter of RFC140 and RFC38. In addition, RFC140 increases PPAR$\gamma$-mediated gene activation, resulting from direct protein-protein interaction of RFC140 and PPAR$\gamma$. Taken together, these findings demonstrate that the regulated expression of RFC140 and RFC38 by specific adipocyte transcription factors is required for the adipocyte differentiation process.
Keywords
Replication factor C; adipocyte differenation; C/EBP; ATF2; PPAR$\gamma$;
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