• Journal of Life Science
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• v.19 no.4
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• pp.514-519
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• 2009

Monocyte chemoattractant protein 1 (MCP1) plays a key role in monocyte /macrophage infiltration to the sub-endothelial space of the blood vessel wall, which is a critical initial step in atherosclerosis. In this study, we examined $interleukin-1{\bate}$ ($IL-1{\beta}$) induced MCP1 expressions via activation of transcription factor $NF-{\kappa}B$ in primary human aorta smooth muscle cells. We determined the effect of several anti-inflammatory agents on $IL-1{\beta}-induced$ MCP1 expression. The pretreatment of luteolin significantly suppressed $IL-1{\beta}-induced$ MCP1 expressions through blocking activation and translocation of $NF-{\kappa}B$ to the nucleus.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.1142-1146
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• 2008

토양수분은 지표수의 유출과정을 설명하는 과정에서 중요인자이며, 생태수문학의 핵심변수이자 기상모형의 결정적인 입력변수이다. 또한 토양수분의 공간적 시간적 특징들은 강우 및 지하수와 토양수분간의 순환 구조를 규명하는데 매우 중요하다. 본 연구에서는 산지사면의 토양수분을 체계적으로 측정하는데 필요한 시스템의 구축을 위한 기초조사 및 사전분석에 대한 연구를 수행하였다. 우수한 토양 수분 측정 장비인 TDR 장비 매설에 앞서 대상유역 선정에 대한 여러 가지 고려사항을 검토하고 수치지형 분석 등을 통한 사전분석을 실시하였다. 대상유역을 선정하기 위해서는 대상유역의 자료획득의 용이함, 지정학적, 시스템 운영적 측면에서의 가용성, 그리고 정밀측량 및 부수적요인 등 여러 요소의 고려가 요구된다. 본 연구에서는 경기도 파주시 적성면 설마리의 설마천 유역내 감악산 범륜사 우측 산지 사면을 측정대상 사면으로, 지정학적 위치, 식생분포, 지질구조 및 심도 등의 토양특성의 고려를 통해서 선정하였다. 또한 대상 사면에 흐름 발생 및 분포를 계산하기 위해서 대상사면의 지표 및 기반암 표고를 정밀 측량하였으며, 기반암 또는 풍화대까지의 깊이를 실측하여 지표면 및 지하면의 수치지형 모형을 구축하였다. 이를 대상사면 및 지하면에 대하여 표고수치지형모형(Digital Elevation Model:DEM)으로 도식한 후 흐름 발생 공간 분포를 계산하였다. 흐름발생공간분포예측은 단방향 알고리즘, 다방향 알고리즘, 흐름 분배 알고리즘 그리고 다중무한방향 알고리즘을 사용하여 지형인자인 기여사면적과 지형습윤지수를 계산하였다. 각 분배알고리즘의 의해 도출된 지형인자들로 인한 흐름발생 공간적 분포특성을 비교하였다. 이는 합리적인 토양수분 측정시스템을 구축하는데 중요한 의사결정 수단으로 판단된다.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.2
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• pp.77-85
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• 2009

In South Korea, seasonal, local and temporal climatic characteristics are variable in rainfall patterns. To design or assess the reliability of hydrosystem, information about the rainfall event under consideration is important. In this process, the complete description of a design storm involves the specification of rainfall duration, depth, and its temporal pattern. Generally, to use an appropriate temporal pattern for a design storm is of great importance in the design and evaluation of hydrological safety for hydrosystem. For purpose of selecting of factors affecting the occurrence of rainfall patterns, Huff's dimensionless method was executed and examined by statistical contingency tables analysis through which the inter-dependence of the occurrence frequency of rainfall patterns with respect to geographical location, rainfall duration and depth, and seasonality is investigated. This analysis result can be used to establish flood policies and to design or assess the reliability of hydrosystem.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.192-192
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• 2018

토양수분량은 가뭄, 홍수 및 갈수 예보, 유출 해석, 작물의 소비수량 산정과 같이 국가 물 관리 및 수자원 계획 및 개발 등의 목적으로 활용되고 있다. 또한, 생태수문학의 중요한 인자로써 식생의 성장 및 변동의 원인과 결과를 동시에 제공한다. 이는 대기의 상호작용 및 총체적인 물 수지와 관련되며 지표면의 침투, 증발산, 오염물의 이송 및 생태계에도 중요한 인자로 활용된다. 토양수분량은 수자원 부존량에 차지하는 비중은 매우 적지만 대상유역의 유출기구 특성을 지배하는 주요인자로 작용하여 홍수량의 규모에 크게 영향을 미친다. 미국 등 여러 선진 외국에서는 오래 전부터 토양수분에 대한 이론적인 개념을 정립하여 토양수분량을 정기적으로 관측해 오고 있다. 우리나라 또한 토양수분량 관측을 하고 있으며, 주로 농업목적으로 토양수분량 관측소가 운영되고 있다. 그 중 기상청에서는 주로 농업기상관측 항목에 포함하여 농업기상관측소를 운영하고 있으며, 농촌진흥청에서는 농작물의 생육 목적으로 토양수분량 자료를 관측하고 있다. 하지만, 유역 단위의 가뭄과 물 순환을 규명하기 위한 연구가 부족하여 최근 국토교통부에서는 유역 단위의 토양수분량 자료생산 연구를 계획하고 있다. 유역 단위의 토양수분관측의 어려움은 주로 산지에서 발생하는 토양수분의 변동이다. 이는 사면의 유출과 식생의 영향 및 기반암의 영향을 받기 때문이다. 본 연구에서는 청미천(수레의산)과 설마천(감악산) 산지 사면에 설치된 유전율식(TDR, Time Domain Reflectometer)장비로 관측된 최근 3년간(2015~2017)의 데이터를 이용하여 경년변화특성을 분석하였다. 분석 결과는 유역의 물 순환을 규명하는데 가장 중요한 연구로써 활용될 것으로 기대된다.

• Clinical and Experimental Reproductive Medicine
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• v.33 no.2
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• pp.125-132
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• 2006

Objective: In the previous study, we complied the differentially expressed genes during early folliculogenesis. Objective of the present study was to identify downstream target genes of transcription factors (TFs) using bioinformatics for selecting the target TFs among the gene lists for further functional analysis. Materials & Methods: By using bioinformatics tools, constituent domains were identified from database searches using Gene Ontology, MGI, and Entrez Gene. Downstream target proteins/genes of each TF were identified from database searches using TF database ($TRANSFAC^{(R)}$ 6.0) and eukaryotic promoter database (EPD). Results: DNA binding and trans-activation domains of all TFs listed previously were identified, and the list of downstream target proteins/genes was obtained from searches of TF database and promoter database. Based on the known function of identified downstream genes and the domains, 3 (HNF4, PPARg, and TBX2) out of 26 TFs were selected for further functional analysis. The genes of wee1-like protein kinase and p21WAF1 (cdk inhibitor) were identified as potential downstream target genes of HNF4 and TBX2, respectively. PPARg, through protein-protein interaction with other protein partners, acts as a transcription regulator of genes of EGFR, p21WAF1, cycD1, p53, and VEGF. Among the selected 3 TFs, further study is in progress for HNF4 and TBX2, since wee1-like protein kinase and cdk inhibitor may involved in regulating maturation promoting factor (MPF) activity during early folliculogenesis. Conclusions: Approach used in the present study, in silico analysis of downstream target genes, was useful for analyzing list of TFs obtained from high-throughput cDNA microarray study. To verify its binding and functions of the selected TFs in early folliculogenesis, EMSA and further relevant characterizations are under investigation.

• Journal of KIISE:Computing Practices and Letters
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• v.15 no.12
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• pp.928-932
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• 2009

Most diseases are caused by complex processes of various factors. Although previous researches have tried to identify the causes of the disease, there are still lots of limitations to clarify the complex factors. Here, we present a disease classification model based on an evolutionary learning approach of combinatorial features using the data sets from the genetics and cohort studies. We implemented a system for finding the combinatorial risk factors and visualizing the results. Our results show that the proposed method not only improves classification accuracy but also identifies biologically meaningful sets of risk factors.

• Journal of Life Science
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• v.13 no.4
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• pp.463-472
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• 2003

Interactions between factor Va (HFVa) and membrane phosphatidylserine (PS) regulate the activity of the prothrombinase complex. I have previously shown that two solvent exposed hydrophobic residues located in the C2-domain, Trp2063 and Trp2064, are required for binding to immobilized PS and for expression of procoagulant activity on membranes containing 5% PS. In order to fully define the functional importance of these two residues I have expressed and isolated recombinant factor Va (rHFVa) W2063A/W2064A double mutant. In contrast to the native protein the two glycoforms resulting from alternative glycosylation of Asn2181 eluted as a single peak with rHFVa1 W2063A/W2064A eluting on the leading edge and rHFVa2 W2063A/W2064A eluting on the trailing edge. The double mutant rHFVa2 W2063A/W2064A expressed little or no procoagulant activity on membranes containing 1-10% mol % PS. In contrast, the procoagulant activity of this mutant was slightly greater than the native protein on membranes containing>18 mol % PS. The binding of rHFVa2 W2063A/W2064A to immobilized phospholipid vesicles was markedly reduced compared to the native protein in a surface plasmon resonance binding assay. I conclude that Trp2063 and Trp2064 are required for high affinity binding of factor Va to PS membranes and that this interaction is necessary for assembly of the prothrombinase complex on membranes containing physiological concentrations of PS.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.1
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• pp.1-9
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• 2015

This paper presents a design method for optimizing the focused beam characteristics, which are mainly determined by the condenser lenses in a scanning electron microscopy (SEM) design. Sharply reducing the probe diameter of electron beams by focusing the condenser lens (i.e., the rate of condensation) is important because a small probe diameter results in high-performance demagnification. This study explored design parameters that contribute to increasing the SEM resolution efficiently using lens analysis and the ray tracing method. A sensitivity analysis was conducted based on those results to compare the effects of these parameters on beam focusing. The results of this analysis on the design parameters for the beam characteristics can be employed as basic key information for designing a column in SEM.

• The Journal of the Korea Contents Association
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• v.16 no.4
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• pp.614-623
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• 2016

Grid(pipe network) design is an important element of Smart Water Grid, which essential to estimate hydraulic parameters such as the pressure, friction factor, friction velocity, head loss and energy slope. Especially, friction velocity in a grid is an important factor in conjunction with energy gradient, friction coefficient, pressure and head loss. However, accurate estimation friction head loss, friction velocity and friction factor are very difficult. The empirical friction factor is still estimated by using theory and equation which were developed one hundred years ago. Therefore, in this paper, new equation from maximum velocity and friction velocity is developed by using integration relationship between Darcy-Weisbach's friction head loss equation and Schlichting equation and regression analysis. To prove the developed equation, smooth pipe data areis used. Proposed equation shows high accuracy compared to observed data. Study results are expected to be used in stability improvements and design in a grid.

• Journal of Environmental Impact Assessment
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• v.25 no.2
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• pp.155-164
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• 2016

Soil has been recognized as an important resource due to its finitude and valuable functions. Therefore, many countries have adopted soil quality related indicators in Strategic Environment Impact Assessment(SEIA). However, Korean SEIA has used limited indicators such as soil contamination until now. In this study, the SEIA on "Development Basic Plan" was modified for considering complex characteristics of soil and applied to "Bogeumjari Housing Supply Plan" and "Urban Management Plan". The performance of the SEIA considering soil characteristics was evaluated using a checklist for assessing consistency with upper plan, adequacy of alternatives, and feasibility of siting. Soil organic matter and soil erosion were derived as main indicators for "Basic Development Plans". The proposed SEIA technique equipped with soil indicators is proved to contribute to the soil resource conservation.