• Current Research on Agriculture and Life Sciences
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• v.31 no.2
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• pp.124-130
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• 2013

The significance of environment change and genetic safety has been recently recognized by many genetically modified (GM) plants. This study was to evaluate the safety of drought-tolerant rice and to identify the environment variance. The GM rice of drought-tolerant rice and four check cultivars were analyzed the data on agronomic characters and principal component in large-GM crop field. There was no significant difference in agronomic characters between the drought-tolerant rice and donor plant, 'Ilmi'. Grain yield showed the standard deviation of the difference, did not significant statistically. Related to grain characters, grain appearance were similar to the drought-tolerant rice and donor plant, 'Ilmi'. In Chemical characters, brown rice of the drought-tolerant rice and a donor plant, 'Ilmi' did difference in starch and protein, however, was similar as 'Ilpum'. These results indicated that drought-tolerant rice may perform to detect genetic safety in GM plants progeny.

• Korean Journal of Plant Taxonomy
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• v.48 no.2
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• pp.143-152
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• 2018

A comparative study of leaf epidermal microstructures in genus Aruncus (two species, five varieties) was carried out using scanning electron microscopy in order to evaluate their significance in terms of taxonomy. All of the leaves of the taxa studied here were amphistomatic with undulate anticlinal walls, and smooth and flat periclinal walls on both surfaces. The size range of the stomata complex is $8.95-21.97{\times}7.50-16.99{\mu}m$: the largest one was found in Aruncus dioicus var. astilboides (average $18.01{\times}13.47{\mu}m$) and the smallest was measured and determined to be A. gombalanus (average $11.11{\times}8.94{\mu}m$). An anomocytic stomata complex was found in all of the studied taxa. The stomatal frequency on average was $27.54/0.05mm^2$; it is highest in A. gombalanus ($60.4/0.05mm^2$) and lowest in A. dioicus var. acuminatus ($11.6/0.05mm^2$). Two types (short stalked capitate glandular trichome and non-glandular trichome) of trichomes are found in the leaves. The non-glandular trichome was divided into three types based on the presence and degree of development of subsidiary cells. Anomocytic stomata of the hypostomatic type and the distribution pattern of capitate glandular trichomes were the major characters in this genus. The stomata size and frequency, the epidermal cell structure, the trichome type and the distribution pattern may have diagnostic importance among the taxa in the genus. Our leaf micromorphological results provide useful information for the taxonomic revision of the genus Aruncus.

• Korean Journal of Plant Taxonomy
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• v.33 no.4
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• pp.339-357
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• 2003

The general morphological characters and chromosome numbers about 7 taxa of Korean Allium sect. Sacculiferum were reviewed. From the results, authors elucidated the key character as well as the range of variations in each taxon, and considered the relationships among taxa in this section. Clear taxonomic treatments also carried out based on the type specimens and the original descriptions. Shape and growing pattern of leaves, size of inflorescences, shape of perianths and tepals, as well as chromosome numbers were key characters in identifying the taxa and in estimating their relationships. On the basis of these characters, A. thunbergii var. thunbergii, A. sacculiferum and A. deltoide-fistulosum were recognised as a distinct species respectively. Hal-la-bu-chu which had been misidentified as A. cyaneum (sect. Reticulato-bulbosa) in Korea was proved to be A. taquetii and belonged to the sect. Sacculi/erum. In addition, A. cyaneum var. deltoides, had been described as a variety of A. cyaneum, was recombined as a variety of A. thunbergii [A. thunbergii var. deltoides (S. Yu, W. Lee et S. Lee) H. J. Choi et B. U. Oh] with the new Korean name of 'Se-mo-san-bu-chu'. In conclusion, Korean sect. Sacculiferum was composed of five species and two varieties. A key to identify the taxa in this section was provided.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.266-266
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• 2004

재래산양은 우리나라 고유의 재래가축이라는 특성과 산양육의 경우 건강보조식품으로서의 인식이 팽배해 왔기 때문에 그동안 체계적인 개량이 이루어지지 못하였다. 동물복제 또는 형질전환 등과 같은 첨단생명공학연구를 위해서는 무엇보다도 oocyte의 대량확보가 가능해야 한다. 본 연구는 재래산양에 있어서 과배란 처리에 의한 oocyte의 회수방법과 회수란의 질적 개선을 확립하기 위하여 과배란 처리 후 회수시간이 난포의 발달과 난자의 회수율에 미치는 영향을 조사하였다. (중략)

• Korean Journal of Animal Reproduction
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• v.20 no.4
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• pp.453-458
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• 1997

It is known that the incorporation of genes into transgenic mice is generally stable and is p passed on to succeeding generations in a Mendelian fashion. In this report, transgenic mice were set as a model to evaluate whether the transgenes are transmitted in a Mendelian principle in a successive generations and how they are tran s smitted into their offspring. A 3.0 kb linear DNA fragment, containing the MMTV LTR, bovine aSI casein cDNA and SV 40 splicing and polyadenylation site; was microinjected into fertilized mouse embryos. The tail DNAs of the resulting pups were subjected to dot and Southern hybridizations to screen transgenic founders. The DNAs of their offspring were anlyzed by PCR to confirm the transmission of the transgene from F0. Out of 72 live pups four pups (5.6%), 3 males and 1 female, were positive for the transgene. The rates of transmission from F0 into F1 were 33.3, 7.7, 0, and 62.5%. Those from F1 into F2 were 63.6, 5.9, and 68.8% and those from F2 into F3 were 85.7, and 88.2%. In this report, the transmission pattern of transgenes in transgenic mice into their offspring was demonstrated. It either follows or does not follow in a Mendelian fashion. Deletion or loss of the transgenes from F0 in some lines became apparant to the succeeding generations.

• Journal of Korean Society of Forest Science
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• v.86 no.3
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• pp.378-390
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• 1997

Ten total populations of Korean fir (Abies koreana Wilson) and Manshurian fir [A. nephrolepis (Traut.) Maxim.] were sampled from south Korea to investigate patterns of intraspecific variation in these species and to evaluate a recognition of the two species. Principal components analysis and cluster analysis were performed both on seed-cone data and on needle morphology data. The characters that contributed most to the separation between A. koreana and A. nephrolepis along three principal components axis were leaf width, length of seed, width of seed wing, length of seed wing, cone width, width of scale, and length of bract tip, but these characters were not diagnostic because of overlap in reality. Therefore, all these characters were not reliable in distinguishing these two taxa including bract position (exerted and recurved vs. exerted and straight). The individuals of A. koreana from Mt. Chi-ri appeared quite unique probably on account of its larger cone size and longer scale tip, while those from Mt. Hal-la of A. koreana were generally distinct from others in terms of their larger seed and seed wing and longer scale width. The Mt. Duk-yu specimens of A. korecana appeared somewhat smaller but more data were needed due to the small sampling size. Generally, the gradual clinal geographic trends made evident by the position of resin ducts in leaves of A. koreana can be detected. The southern populations, Mt. Hal-la (an insular population) were generally distinct from the northern populations (Mt. Chi-ri, Mt. Ga-ya and Mt. Duk-yu) in terms of their position of resin duct (medial, within mesophyll vs marginal, close to epidermis : 100% vs 75 or 50%). Although no sharp boundary separating these two species could be detected based on cone and needle morphology, the observed clinal pattern was distinct in northern populations of A. koreana and southern population of A. nephrnlepis. In a preceding study of the flavonoids variation of 20 species in eastern Asia, flavanone (5-deoxyflavanone) was found to be characteristic of A. faxoniana Rehder et Wilson, A. georgei Orr of China and A. koreana of Korea. A. faxoniana, which is assumed to be primitive species, has position of resin duct relative to both the medial and the marginal, while A. georgei and A. koreana are identified by marginal position of resin duct. With respect of foliar flavonoids chemistry, A. koreana was distinct from A. nephrolepis : the southmost samples (Mt. Hal-la and Mt. Chi-ri) contained additional flavonoids derivatives (mainly flavanone) that were not found in the northmost samples of A. nephrolepis except a few individuals from Mts. Seo-rak and Tae-bak populations of Kwang-won province. The presence of A. koreana type flavonoids in two Chinese species suggested that position of resin duct may be a phyletic character. Abies koreana including two Chinese taxa, exhibited the most elaborate and specialized flavonoids profile within the Abies in eastern Asia. Contrary to our initial expectations, the apparent intermediates between A. nephrolepis and A. koreana in Duk-yu and Ga-ya mountains were found. The pattern of variation on position of resin duct and flavonoids chemistry in these populations of A. kareana suggested that genetic interchange or natural hybridization had occurred between these two species. The evidence needed to resolve the status of this taxon is still inconclusive in our opinion until intermediate individuals from Mts. Duk-yu and Ga-ya show indication of hybridization between the two species.

• Korean Journal of Breeding Science
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• v.42 no.1
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• pp.40-49
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• 2010

This study was conducted to examine genetic variation on leaf characteristics of Zelkova serrata populations. Leaf samples were obtained from eighteen populations and fourteen leaf characteristics such as leaf blade length, leaf width, leaf area and etc. were measured. In all leaf characteristics, there were significant differences among populations and among individuals within population. Most variance of leaf characteristics was contributed to among individuals within population except for length from leaf base to maximum width (x3) and the ratio of x3 to leaf blade length (x10). The relatively high variations of leaf characteristics were found at Gurye, Jungeup and Gyungju populations while Youngwol, Cheongsong, Youngchun populations showed the lower variation. There was high correlation among leaf characteristics related to leaf size, but not among the variables of ratio between leaf characteristics. Length from leaf base to maximum width(x3) and the ratio of x3 to x10 showed significant positive correlation with latitude and altitude of populations, which reflect the differences of mean annual temperature among populations. Mean annual rainfall of populations showed negative correlation with leaf blade length, leaf width, length from apex to first serration and leaf area. Four principal components (PC) were deduced from principal component analysis, which explain the 88.5% of total variance of leaf characteristics. Leaf area, length from leaf base to maximum width, serration number and petiole length showed the highest contribution to PC1, PC2, PC3, PC4, respectively. According to cluster analysis, the populations of Z. serrata were divided into two groups, which reflect the difference of mean annual temperature between groups. Within group, however, specific tendency of clustering was not observed among populattions.

• Korean Journal of Plant Taxonomy
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• v.46 no.1
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• pp.13-32
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• 2016

Neillieae was traditionally recognized as a small tribe in Rosaceae, which consists of Neillia, Stephanandra, and Physocarpus. Recently, Stephanandra was merged into Neillia based on molecular phylogenetic analyses, meaning that Neillieae now contains Neillia and Physocarpus. The genus Neillia is distinguished from Physocarpus by ovate to lanceolate leaves with acuminate to caudate apices, racemose or paniculate inflorescences, and a unicarpellate (rarely bicarpellate) gynoecium. Plants of Neillia are distributed from the Himalayas across China and Korea to Japan in the east, and south to Indonesia. This study provides a taxonomic treatment of Neillia based on a morphological examination of herbarium specimens, including types, and field observations, as the second part of the taxonomic revision of the tribe Neillieae. A summary of the phylogeny of Neillia, keys to the species, nomenclatural reviews, descriptions, distribution maps, and lists of specimens examined are provided. Twelve species with ten varieties are recognized in Neillia. A lectotype was designated here for the following species: N. sinensis f. glanduligera and N. thyrsiflora.

• Journal of Plant Biology
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• v.35 no.4
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• pp.385-392
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• 1992

여뀌속 Tovara절 식물은 매우 복잡한 양상의 형태변이를 나타내기 때문에 각 분류군의 한계 및 위치 성정, 계통적 유연관계 해석에 있어 많은 혼란이 나타나고 있다. 따라서 본 연구에서는 본 절 분류군에서 나타나는 형태 변이의 양상을 수리분류학적으로 분석하여, 각 분류군의 한계를 명확히 설정하고 그 분류학적 타당성을 검토하고자 하였다. 본 절 분류군의 주요 식별형질에 대한 주성분 분석을 수행한 결과, 본 절은 형태적으로 크게 P. virginianum, P. filiforme, P. neofiliforme의 3분류군으로 구분되며, 이들은 주로 잎의 형태에 의해 식별되는 것으로 밝혀졌다. 또한 P. virginianum var. glaberrimum은 그 분류학적 타당성이 없는 것으로 나타났으며, Tovara (=Polygonum) virginiana var. kachina는 형태적으로 볼 때 P. neofiliforme의 변종으로 취급하는 것이 타당한 것으로 판명되었다. Polygonum virginianum, P. filiforme, P. neofiliforme는 그 flavonoid 조성에 있어서도 명확한 차이를 나타내며, 이러한 결과를 종합해 볼 때 이들은 각각 독립된 종으로 인식하는 것이 타당한 것으로 판단된다.

• Microbiology and Biotechnology Letters
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• v.18 no.1
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• pp.94-97
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• 1990

The restriction cleavage map of multi-copy recombinant plasmid, pJY502 (5.5 kb), carrying the thiostrepton resistance gene (tsr) was determined. Comparison of the restriction pattern with that of Streptomyces plasmids previously demonstrated that pJY502 was novel. The plasmid pJY502 had a broad host range in Streptomyces and contained single BgtII site for cloning purpose. Transformation frequency of pJY502 was $2.2 \times 10^5$ in S. lividans. E. coti-Streptomyces bifunctional plasmid, pJY504, was also constructed.