Properties of Recombinant Derivatives of pJY501, A Multi-copy Streptomyces plasmid

Multi-copy Streptomyces 플라스미드, pJY501의 재조합 유도체의 특성

The restriction cleavage map of multi-copy recombinant plasmid, pJY502 (5.5 kb), carrying the thiostrepton resistance gene (tsr) was determined. Comparison of the restriction pattern with that of Streptomyces plasmids previously demonstrated that pJY502 was novel. The plasmid pJY502 had a broad host range in Streptomyces and contained single BgtII site for cloning purpose. Transformation frequency of pJY502 was $2.2 \times 10^5$ in S. lividans. E. coti-Streptomyces bifunctional plasmid, pJY504, was also constructed.

Thiostrepton 내성 유전자(tsr)를 포함하는 multicopy 재조합 플라스미드 pJY502(5.5kb)의 제한효소 지도를 비교해본 결과 pJY502는 새로운 플라스미드로 확인되었다. pJY502는 Streptomyces에서 넓은 host range를 나타내었으며 cloning에 사용할 수 있는 단일 BglII 제한효소 인식부위를 갖고 있었다. pJY502의 형질전환 빈도는 S.lividans에서 $2.2 \times 10^5$이었다. 또한 E.coli-Streptomyces bifunctional 플라스미드 pJY504을 제조하였다.