• Microbiology and Biotechnology Letters
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• v.46 no.1
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• pp.18-28
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• 2018

In this study, the antioxidant activities and cytoprotective effects against oxidative stress of Lonicera japonica Thunb. 50% ethanol extract and ethyl acetate fraction were investigated. Using the 1,1-diphenyl-2-picrylhydrazyl assay, the free radical scavenging activity (FSC50) of L. japonica Thunb. 50% ethanol extract and ethyl acetate fraction was determined as 152.00 and $77.25{\mu}g/ml$, respectively. To measure the reactive oxygen species (ROS) scavenging activity, the total antioxidant capacity (OSC50) was determined by using a luminol-dependent chemiluminescence assay. The antioxidant activity of the ethyl acetate fraction ($0.33{\mu}g/ml$) was approximately four times stronger than that of the 50% ethanol extract ($1.12{\mu}g/ml$). The protective effect against $^1O_2$-induced cellular damage of human erythrocytes (${\tau}_{50}$) was 46.0 min at $10{\mu}g/ml$ of the 50% ethanol extract and 52.3 min at $1{\mu}g/ml$ of the ethyl acetate fraction. We also investigated the cytoprotective effects against oxidative stress induced by $H_2O_2$ and the intracellular ROS scavenging activity in response to UVB irradiation and found that the extract and fraction protected human skin cells from damage and reduced ROS. These results confirmed that L. japonica Thunb. was a valuable plant-derived natural antioxidant with potential for development as an antioxidative functional ingredient.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.4
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• pp.222-228
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• 2006

This study was conducted to evaluate the heavy metal content (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb, Zn) of forest soil and of Lonicera japonica and to understand the correlation in contents of heavy metal between forest soil and L. japonica near the Onsan industrial region. The content of As, Cd, Cu, Pb and Zn was higher in the industrial region than in forest regions, but re, Mn and Ni content was higher in forest regions than in the industrial region. Among heavy metals, the content of Cd, Cu, Pb and Zn in leaf, stem and root of L. japonica growing near industrial regions was significantly higher (p<0.05) than in forest regions. The content of As, Cd, Cu, Pb and Zn in L. japonica tissues showed a positive or negative correlation with those in forest soils. Correlation coefficients of Cu content between forest soil and organs of L. japonica ranged from 0.93 to 0.99 (p<0.01). It was concluded that L. japanica could be used in heavy metal (Cd, Cu, Pb, and Zn) decontamination of forest soils of industrial regions.

• Landscaping Tree
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• v.40 no.9_10
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• pp.25-26
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• 1997

• Korean journal of applied entomology
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• v.58 no.3
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• pp.203-207
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• 2019

Bemisia tabaci (Hemiptera: Aleyrodidae) is one of the major insect pests causing economic damages to over 900 different crops in the world. Especially, B. tabaci JpL (L. japonica) is known as a species living only in Japan and Korea so far, and mainly in Lonicera japonica and specific plant species. This study was conducted to investigate the occurrence and spread of B. tabaci JpL on Jeju Island in 2019. Of the total 25 areas surveyed, 1,003 individuals of B. tabaci JpL adults were collected from 24 L. japonica and one Conyza sumatrensis. All populations identified belonged to the JpL species. The result of this study showed that the population of B. tabaci JpL has increased at very high speeds to date compared to the previous reports in Korea.

• Korean Journal of Agricultural and Forest Meteorology
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• v.4 no.3
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• pp.159-163
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• 2002

This study was analyzed to photosynthetic pigment concentrations and changes of SOD activities on seven species of liana of A. heterophylla, P. scandens, V. thunbergii, P. tricuspidata, C. trilobus, L. japonica and T. kirilowii, and two species of E. arvense and A. princeps of non climbing plants. Concentrations of chlorophyll a and b, total chlorophylls and total carotenoids of P. tricuspidata in 100 ppb ozone site were the most increased. It was the most increased to P. scandens in ratio of chlorophylls and carotenoids, and E. arvense in ratio of chlorophyll a and b. There was difference to ratio of chlorophyll a and b of liana and non liana. At ratio of chlorophyll a and b of 100 ppb ozone site and the control it was more sensitive to chlorophyll a than chlorophyll b, and P. tricuspidata was the most sensitive at comparing with species, and it was more sensitive to liana than non liana. In SOD activities A. princeps was the most increased to 3535.7 unit/g, and P. scandens was the fewest increased to 109.3 unit/g, and A. heterophylla was only decreased to 131.7 unit/g in comparing to 100 ppb ozone sites and the control.

• 한국약용작물학회:학술대회논문집
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• 2008.05a
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• pp.140-141
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• 2008

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.1
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• pp.67-72
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• 2020

In this study, we attempted to obtain an active ingredient that inhibits the production of matrix metalloproteinase-1 (MMP-1) that breaks down collagen in human skin fibroblasts. More than 50 plant extracts were screened, and Lonicera japonica was selected for this study. The stem of L. japonica was extracted with 70% ethanol and fractions with solvents in the order of hexane, ethyl acetate, and butanol. MMP-1 production were significantly inhibited at the concentration of 50 ㎍/mL of the ethyl acetate layer and 200 ㎍/mL of the butanol layer. To get a fraction containing all of these effective components, 80% ethanol fraction (LJ F80) was obtained through HP20 resin column chromatography. The reference substance, loganin and LJ F80 inhibited dose-dependently MMP-1 production. At the same concentration, LJ F80 showed a higher inhibitory effect than loganin. The stability of this fraction was analyzed with HPLC while kept storing at 4 ℃, room temperature, and 40 ℃, for 16 week. The stability was maintained as ± 10% of initial value with reference loganin. Therefore, it is thought that LJ F80 of L. japonica may be used to improve wrinkles.

• Journal of Korean Society of Forest Science
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• v.99 no.3
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• pp.432-438
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• 2010

This study was carried out to understand the effect on soil chemical property, ion contents, and growth of Lonicera japonica by treatment of simulated acid rain and wood vinegar. It was treated total 30 times by one time per 2 weeks during 150 days from June to October dividing to various pH level of simulated acid rain (each pH 5.6, 4.5, 3.5 and 2.5) and the control site (pH 6.3). The wood vinegar diluted with 500 times was also treated total 10 times by one time per 2 weeks at the experimental sites. The soil pH was reduced as pH level of simulated acid rain was decreased. The organic content, total nitrogen, and Av. $P_2O_5$ in soil showed a tendency to increment. However, the soil improvement effect was exposed to be insufficient when a wood vinegar was treated. In the component of the plant body, $SO_4{^2}$ and $NO_3{^-}$ increased due to the simulated acid rain treatment and the increment ratio was reduced in the wood vinegar treatment plot. In addition, the cation contents showed a tendency to variation due to pH of simulated acid rain. And it showed more increment in the leaves than the root. The early fallen leaves and growth inhibition of L. japonica showed up after the simulated acid rain treatment but the growth of L. japonica increased after the wood vinegar treatment.

• FLOWER RESEARCH JOURNAL
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• v.17 no.2
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• pp.88-92
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• 2009

This experiment was conducted to find out the effects of pinching on shoot growth, flowering and covering of Lonicera sempervirens growth in containers. 180 cm high pinching of plants increased shoot length compared to 60 cm low pinching and decreased lateral shoot numbers, but there was no difference in branch numbers by the pinching methods. Covering rates of the pergola were 85% with 60 cm low pinching, 74% with 120 cm middle pinching, and 62% with 180 cm high pinching. The pinched plants continued to flower from the middle of May to the middle of September. The first flowering time was in the middle of May in low pinching and in the early of June in middle and high pinching. The lower pinching height and the earlier pinching time induced earlier flowering time, and the peak flowering of pinched plants was in mid-August. To cover over 85% of the pergola screen with 3.0 m wide and 2.2 m high, two plants per 1 m needed to grow and to pinch at 60 cm height.

• Journal of Practical Agriculture & Fisheries Research
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• v.24 no.4
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• pp.18-29
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• 2022

Five species of plants (Clerodendrum trichotomum Thunb., Angelica dahurica (Fisch. ex Hoffm.) Benth. & Hook. f. ex Franch. & Sav., Caryopteris incana (Thunb. ex Houtt.) Miq., Lonicera japonica Thunb., and Parasenecio auriculatus var. matsumurana Nakai) native to the clean area of Mt. Jiri were selected. The collection period was from May to September 2021, and the five species plants were collected in their native habitats with flowers in full bloom. The collected plants were extracted with 70% EtOH, and 17 kinds of polyphenol components were analyzed. Next, flowers, leaves, stems, and roots were separated from plants, extracted with 70% EtOH for each part and experiments were conducted on DPPH, ABTS, total polyphenols, and total flavonoids. The results are as follows. 1. It was found that there were a total of 8 kinds of polyphenols contained in 5 species of plants that are native to Mt. Jiri. Among the polyphenol components, chlorogenic acid was contained in 4 species of plants, and caffeic acid was contained in 2 species of plants. 2. As a result, the DPPH radical scavenging activity was the best in the stem of P. auriculata and the C. trichotomum was good regardless of the specific part. It was found that the activity-scavenging activity was good in the flowers of A. dahurica and the leaves of L. japonica. 3. The highest ABTS radical scavenging activity was C. trichotomum Thunb., whose EC₅₀ value was 38.73~66.28ppm. Next, the leaves and stems of L. japonica Thunb., A. dahurica and P. auriculata, and the leaves and stems of C. incana appeared in that order. 4. The highest total polyphenol content was 154.83mg GAE/g in the leaves of C. trichotomum, followed by about 130mg GAE/g in the flowers of C. trichotomum and P. auriculata. The lowest was 26.27mg GAE/g in the stems of A. dahurica.