Browse > Article
http://dx.doi.org/10.23097/JPAF:2022.24(4).18

Analysis of Ingredients and DPPH, ABTS Activity for the Development of Cosmetic Raw Materials using 5 Kinds of Plants Native to Mt. Jiri  

Youn Ok, Jung (Sooy-K Bio Lab, Research and Development)
Bo Kyung, Kang (B.K Bio)
No Bok, Park (Department of Floriculture, Korea National College of Agriculture and Fisheries)
Publication Information
Journal of Practical Agriculture & Fisheries Research / v.24, no.4, 2022 , pp. 18-29 More about this Journal
Abstract
Five species of plants (Clerodendrum trichotomum Thunb., Angelica dahurica (Fisch. ex Hoffm.) Benth. & Hook. f. ex Franch. & Sav., Caryopteris incana (Thunb. ex Houtt.) Miq., Lonicera japonica Thunb., and Parasenecio auriculatus var. matsumurana Nakai) native to the clean area of Mt. Jiri were selected. The collection period was from May to September 2021, and the five species plants were collected in their native habitats with flowers in full bloom. The collected plants were extracted with 70% EtOH, and 17 kinds of polyphenol components were analyzed. Next, flowers, leaves, stems, and roots were separated from plants, extracted with 70% EtOH for each part and experiments were conducted on DPPH, ABTS, total polyphenols, and total flavonoids. The results are as follows. 1. It was found that there were a total of 8 kinds of polyphenols contained in 5 species of plants that are native to Mt. Jiri. Among the polyphenol components, chlorogenic acid was contained in 4 species of plants, and caffeic acid was contained in 2 species of plants. 2. As a result, the DPPH radical scavenging activity was the best in the stem of P. auriculata and the C. trichotomum was good regardless of the specific part. It was found that the activity-scavenging activity was good in the flowers of A. dahurica and the leaves of L. japonica. 3. The highest ABTS radical scavenging activity was C. trichotomum Thunb., whose EC50 value was 38.73~66.28ppm. Next, the leaves and stems of L. japonica Thunb., A. dahurica and P. auriculata, and the leaves and stems of C. incana appeared in that order. 4. The highest total polyphenol content was 154.83mg GAE/g in the leaves of C. trichotomum, followed by about 130mg GAE/g in the flowers of C. trichotomum and P. auriculata. The lowest was 26.27mg GAE/g in the stems of A. dahurica.
Keywords
C. trichotomum; A. dahurica; Polyphenol; DPPH; ABTS;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 문수찬. 2022. 포공영, 금은화, 진피 혼합물의 항산화 및 항염증 효과에 관한 연구. 대전대학교대학원. 석사학위논문 
2 임융호. 2014. 식물유래 폴리페놀 배당체 기반 화장품 소재 개발. 건국대학교 대학원 
3 정민영. 2001, 누리장나무 잎의 항산화 성분. 중앙대학교대학원 박사학위논문 
4 Agung Nugroho, Choi JS and Park HJ. 2017. Quantification of Caffeoylquinic Acids and the Effect of the Cacalia hastata var. orientalis Extract on Acetylcholinesterase Activity and Peroxynitrite Concentration. Kor. J. Pharmacogn. vol. 48(3) : p.243-247 
5 Choi CW, Jung HA, Kang SS and Choi JS. 2007. Antioxidant constituents and a new triterpenoid glycoside from Flos Lonicerae. Arch Pharm Res. vol. 30(1) : p.1-7    DOI
6 Choo HN, Lee SI, Kim JS, Jeong JK. 2015. Effect of Clerodendron trichotomum Thunberg tea on anti-hypertension. Kor. J. Herbol. 2015；30(4): p.129-135    DOI
7 Gao J, Igalashi K, Nukina M. 1999. Radical scavenging activity of phenylpropanoid glycosides in Caryopteris incana. Biosci Biotechnol Biochem. vol. 63 : p.983-988    DOI
8 Gao J, Igarashi K, Nukina M. 2000. Three new phenylethanoid glycosides from Caryopteris incana and their antioxidative activity. Chem Pharm Bull vol. 48 : p.1075-1078    DOI
9 Ha SH, Ha ES, Oh YS, Jeong MH, Kim S, Seo HM. 2014. A composition for antioxidating, whitening and improving wrinkle comprising extracts of nursery spiraea. KOR Patent 1020140187919 
10 Hu YY, Tian JP, Zhang JZ. 2012. Determination of the total flavonoids in Flos Lonicera in Hainan province. Medicinal Plant, vol.3(11) : p.10-12 
11 Jeong YT, Jeong SC, Hwang JS and Kim JH. 2015. Modulation effects of sweroside isolated from the Lonicera japonicaon on melanin synthesis. Chem. Biol. Interact. vol. 238 : p. 33-39    DOI
12 Joo EY and Kang WJa. 2005. Analysis on the Components of the Angelica dahurica Root. Korean J. Food Preserv. vol. 12(5) : p.476-481 
13 Kim JH, Song HN, Ko HC, Lee JY, Jang MG and Kim SJ. 2017. Anti-oxidant and Anti-inflammatory Properties of Clerodendrum trichotomum Leaf Extracts. Journal of Life Science vol. 27(6) : p.640-645    DOI
14 Kim PS. 2011. Whitening Effects of Angelica dahurica Radix Ethanol Extract, Journal of the Korea Academia-Industrial Coop. Society. Vol. 12(9) : p. 4038-4045    DOI
15 Kim PS, Lee TJ, Kim YH and Kim JS. 2011. Anti-oxidative Activities of Angelica dahurica Radix Ethanol Extract, Journal of the Korea Academia-Industrial Coop. Society. Vol. 12(10) : p. 4378-4384    DOI
16 Kim SJ. 2008. Composition and Cell Cytotoxicity of Essential Oil from Caryopteris incana Miq. in Korea, Korean Soc. Appl. Biol. Chem. vol. 51(3) : p.238-244 
17 Kim CM, Kwon YS and Choi SY. 1995. Antithrombotic effect of the BuOH soluble fraction of Angelica dahurica root. Kor. J. Pharmacogn., vol. 26(1) : p.74-77 
18 Kim SH, Kang SS and Kim CM. 1992. Coumarine glycosides from the roots of Angelica dahurica. Arch. Pharm. Res. 15: 73-77.    DOI
19 Kim DK, Lim JP, Yang JH, Eom DO, Eun JS and Leem KH. 2002. Acetylcholinesterase inhibitors from the roots of Angelica dahurica. Arch. Pharm. Res., vol. 25(6) : p.856-859    DOI
20 Kim HS and Chi HJ. 1990. Studies on essential oils of plants of Angelica genus in Korea(III). Kor. J. Pharmacogn., vol. 21 : p.121-125 
21 Kim SK, Kim YH, Kang DK, Chung SH, Lee SP and Lee SC. 1998. Essential oil content and composition of aromatic constituents in leaf of Saururus chinensis, Angelica dahurica and Cnidium officinale. Kor. J. Medicinal Crop Sci. 6: 299-304. 
22 Kim SJ, Kim YH, Kang DK, Chung SH, Lee SP and Lee SC. 1998. Essential oil content and composition of aromatic constituents in leaf of Saururus chinensis, Angelica dahurica, and Cnidium officinale. Korean J. Medicinal Crop Sci., 6 : p.299-304 
23 Kimura K, Sakamoto Y, Fujisawa N, Uesugi S, Aburai N, Kawada M, Ohba S, Yamori T, Tsuchiya E and Koshino H. 2012. Cleavage mechanism and anti-tumor activity of 3,6-epidioxy-1,10-bisaboladiene isolated from edible wild plants. Bioorg. Med. Chem. vol. 20 : p.3887-3897.    DOI
24 Kwon YS and Kim CM. 1992. Coumarine glycosides from the roots of Angelica dahurica. Kor. J. Pharmacogn. 23: 221-224. 
25 Lee JE, Lee EH, Kim BO, Cho YJ. 2017. Biological activities of extracts from Caryopteris incana Miq., J Appl Biol Chem vol. 60(1) : p.61-68    DOI
26 Lee JS, Myung CH, Lee JE, Jo MR, Kim HS, Lee NY, Woo HD, You JE, Jo H and Hwang JS. 2019. Anti-Inflammatory and Moisturizing Effect of Centella Extracts Fermented in Jeju Lava Water, J. Soc. Cosmet. Sci. Korea vol. 45(4) : p.363-372    DOI
27 Ma RZ, Zhang YC and Ye W. 2010. Chemical constituents from n-butanol extract of the stems of Lonicera japonica. J. China Pharm. Univ., vol. 41(4) : p.333. 
28 Lee JW, Bae JJ and Kwak JH. 2016. Glycosides from the Flower of Clerodendrum trichotomum, Kor. J. Pharmacogn. vol. 47(4) : p.301-306 
29 Lee JW, Kang SC, Bae JJ, Lee KB and Kwak JH. 2015. Flavonoids from the Flower of Clerodendrum trichotomum. Kor. J. Pharmacogn. 46(4) : p.289-294 
30 Lee YS. 2007. Antioxidative and Physiological Activity of Extracts of Angelica dahurica Leaves. Korean J. Food Preserv., vol.14(1) : p. 78-86 
31 Miyazawa M, Kawauchi Y, Utsumi Y and Takahashi T. 2010. Character impact odorants of wild edible plant-Cacalia hastata L. var. orientalis-Used in Japanese traditional food. J. Oleo Sci. vol. 59 : p.527-533    DOI
32 Nho JH, Jung HK, Lee MJ, Jang JH, Sim MO, Jung JK, Jung DE, An BK and Cho HW. 2017. Inhibitory Effect of Cynaroside Isolated from Lonicera japonia Thunb on Doxorubicin-induced Necrosis in Human Renal Proximal Tubular HK-2 Cells. Korean J. Medicinal Crop Sci. 25(5) : 322 - 327    DOI
33 Pae HO, Oh HC, Yun YG, Oh GS, Jang SI, Hwang KM, Kwon TO, Lee HS and Chung HT. 2002. Imperatorin, a furanocoumarin from Angelica dahurica (Umbelliferae), induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia, HL-60 cells. Pharmacol. Toxicol., vol.91(1) : p.40-48    DOI
34 Ryu SY, Kim JC, Kim YS, Kim HT, Kim WK, Choi GJ, Kim JS, Lee SW, Heor JH and Cho KY. 2001. Antifungal activities of coumarins isolated from Angelica gigas and Angelica dahurica against plant pathogenic fungi. The Korean J. Pesticide Science vol. 5(3) : p.26-35 
35 Shanga X, Pana H, Li M, Miaoa X and Ding H. 2011. Lonicera japonica Thunb.: Ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine. J. Ethnopharmacol., vol. 138(1) : p.1-21    DOI
36 Wang YD, He Y, Dai Z, Kang S, Zhang J and Ma SC. 2016. A comparative study on bioactive constituents in different parts of Lonicera japonica determined by HPLC ESI-MS(n). J. Asian Nat. Prod. Res., vol. 18(10) : p. 988 
37 Sun X, Sun GB, Sun XB. 2011. Protective effects of cynaroside against H2O2- induced apoptosis in H9c2 cardiomyoblasts. J. Cell. Biochem. 112 : p.2019-2029    DOI
38 Tian P, Li C, Wang J and Kang W. 2011. Analysis on volatiles in Clerodendrum trichotomum Thumb. by headspace solid phase micro-extraction coupled with GC-MS. Tianran Chanwu Yanjiu Yu Kaifa 23 : p.1077-1079 
39 Wang LJ. 2010. The study progress of Lonicera japonica. Medical Information. 8 : p.2293-2296. (in Chinese) 
40 Wang LQ. 2008c. Studies on antiviral effect and immunopotentiating activity of Lonicera japonica Thunb, and flos lonicerae in vitro. Agricultural University of Henan. Mater thesis (in Chinese). 
41 Xiong J, Li S, Wang W, Hong Y, Tang K and Luo Q. 2013. Screening and identification of the antibacterial bioactive compounds from Lonicera japonica Thunb. leaves, Food Chem., vol. 138(1) : p.327-333   DOI