• Korean Journal of Poultry Science
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• v.19 no.1
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• pp.13-16
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• 1992

Avian infectious bronchitis virus(IBV) was propagated in SPF eggs and purified by sucrose density gradient centrifugation in order to prepare the antigen. Several fusions were made between mouse myeloma cells and spleen cells from BALB/c mouse immunized with IBV antigen and two hybridoma clones producing specific monoclonal antibody(MCA) against the IBV were established. The MCAs were classified as IgG type and revealed no neutralizing and hemagglutination inhibition activity. Using the MCA IBV antigen was detected by IFA method in tracheal smears made from chickens infected with IBV during the experimental period of 10 days.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.29-29
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• 2002

참오갈피(Acanthopanax pedunculus)는 국내 고유한 자생 종이며 현재 몇 그루 만이 존재하는 것으로 추정된다. 이 수종은 대체적으로 다른 오갈피 수종에 비해 잎 및 열매는 물론 식물 전체 부위가 다소 커서 경제성이 높다고 판단된다. 본 실험에서는 참오갈피의 세포배양 기술을 이용한 묘목 및 약용원료의 산업적 대량 생산 기술을 개발하고자 수행 하였다. 참오갈피 접합자배를 적출하여 무균적으로 배양한 다음 자엽단계로 성숙한 이들 접합자배를 1 mg/1 2,4-D가 첨가된 MS 배지에 배양하여 배형성 캘러스를 유도하였다. 배형성 세포의 유도율은 약 72%에 달했다. 유도된 배형성 캘러스는 2,4-D가 첨가된 같은 고체 및 액체 배지에서 계대배양하여 배형성 캘러스 및 세포를 유지하였다. 배형성 캘러스를 2,4-D가 첨가되지 않은 배지에 옮겨주면 이들 세포로부터 체세포배가 발생되었다. 약 2개월의 기간을 거쳐 자엽단계로 성숙된 체세포 배는 발아되지 않기 때문에 5 mg/1 GA$_3$가 첨가된 1/2 MS 배지에 옮겨 발아 및 식물체로 재생 시켰다. 약 7 cm 크기로 재생된 식물체를 인공토양에 옮겨 1달간 순화시키고 난 후 토양에 옮겼을 경우 87%가 생존하였다. 기내에서 유도된 참오갈피 식물체는 특별한 처리를 하여주지 않아도 뿌리로부터 배발생 세포를 형성 할 수 있었다. 따라서 이들 식물체로부터 배발생세포 유도는 세포주의 보존 및 증식에 효과적으로 이용될 수 있었다. 한편 참오갈피 세포는 5-10 리터 바이오리엑터 배양을 통하여 배형성세포, 자엽단계의 배, 유식물의 대량생산의 가능성을 확인하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.27 no.5
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• pp.453-456
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• 2001

Background: Autogenous alveolar bone cell transplantation may be suitable for tissue engineering for alveolar bone reconstruction. This study aimed to isolate human alveolar bone-derived cells (HABDCs) and to evaluate the ability of collagen gels to support HABDC proliferation and differentiation for human alveolar bone tissue engineering applications. Method: Cultures of primary HABDCs were established from alveolar bone chips obtained from 10 persons undergoing tooth extraction. These cells were expanded in vitro until passage 3 and used for the in vitro characterization of HABDCs and the in vitro analysis of collagen gels for alveolar bone tissue engineering. Results: Of the 10 attempts made to obtain HABDC cultures, eight were successful. HABDCs expressed the osteoblastic phenotype characterized by alkaline phosphatase activity, osteocalcin expression and the mineralization of the extracellular matrix in vitro. When seeded on collagen gels, HABDCs penetrated into the collagen gel matrices and proliferated inside the gels. Significantly, when HABDCs were embedded into the gels, collagen fibers and mineralization were produced within the gels. Conclusion: This study demonstrates the feasibility of using cultured HABDCs and collagen gels for human alveolar bone tissue engineering applications.

• Proceedings of the Materials Research Society of Korea Conference
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• 2009.05a
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• pp.46.1-46.1
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• 2009

우리 몸의 뼈를 재료적인 측면으로 보면, 주로 나노 크기의 콜라겐과 아파타이트로 이루어 져 있는 복합체이다. 때문에 최근 생체 모사적인 측면에서 나노 크기의 생체 활성 재료를 이용하여 골 재생 촉진이 우수한 지지체를 제작하고자 하는 많은 연구 들이 진행되고 있다. 이러한 나노 크기의 재료는 일반적인 마이크로 크기의 생체 재료에 비해 표면적이 월등히 크기 때문에 생체 활성 (bioactivity)이 우수하다고 알려져 있으며, 이를 골 재생용 지지체의 구성 재료로 사용하였을 경우 기계적 강도 또한 향상 시킬 수 있다고 알려져 있다. 따라서 본 연구에서는 나노 크기의 HA, CaSiO3 등 다양한 나노 생체 활성 입자들을 침전법 (precipitation method)을 통하여 제조하였으며, 이를 이용하여 골 재생 촉진을 위한 3차원 지지체를 제조 하였다. 또한 기존의 마이크로 크기의 생체 재료로 제작된 지지체와의 생물학적, 기계학적 비교 평가를 통하여 나노크기의 재료의 우수성을 입증하고자 하였다. 결론적으로, 나노 크기의 재료로 제작된 골 재생용 지지체의 경우 기존의 마이크로 크기의 재료로 제작된 지지체보다 골세포의 부착, 증식 및 분화능이 우수하였고, 지지체의 기계적 강도 또한 향상됨을 알 수 있었다. 이를 통하여 나노 크기의 생체 활성재료는 골 재생 촉진을 위한 지지체 제작에 응용 가능성이 높음을 확인 할 수 있었다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.2
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• pp.175-184
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• 2019

The epidermal growth factor (EGF) has a intrinsic function of inducing growth and proliferation of cells through interacting with cell membrane receptors in human epidermis and dermis layer. These functions of EGF are used as a main ingredient for wound healing medicines and anti-aging cosmetics. As a cosmetic ingredient, the EGF has a problem in exhibiting its natural efficacy due to the lack of the ability to penetrate through the stratum corneum, which is known as the skin barrier. In this study, a recombinant human epidermal growth factor ($MTD_{151}-EGF$) fused with the macromolecule transduction domain $(MTD)_{151}$ with the skin penetration ability was developed to improve the skin penetration efficiency of the EGF. Expression of $MTD_{151}-EGF$ was performed in E. coli transformed with a vector encoding the $MTD_{151}-EGF$ gene and then purified. The purified $MTD_{151}-EGF$ was evaluated using cell proliferation assay, cytotoxicity test and skin penetration test by franz diffusion cell assay and artificial skin. Cell proliferation activity of $MTD_{151}-EGF$ purified to high purity of 99% or above was equivalent to the EGF or better, and cytotoxicity was not observed. In addition, the $MTD_{151}-EGF$ showed an excellent penetration efficiency compared to the EGF in the skin penetration test with EGF and $MTD_{151}-EGF$ labeled by FITC in an artificial skin penetration model. Based on the quantitative analysis of the penetrating substance using franz diffusion cell assay, the amount of penetration was about 16 times more than that of EGF. These results can be regarded as an effective alternative to improve the existing physical transdermal penetration method related to the use of various active ingredients for cosmetics.

• Journal of Korean Society of Forest Science
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• v.97 no.6
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• pp.570-575
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• 2008

This study was carried out to provide basic information on physiological changes of 4-year-old seedlings of Pinus thunbergii inoculated with pine wood nematodes by analyzing changes of symptom development, stem and needle water content and nematode populations in stem. Twenty days after inoculation, needles were discolored as an external symptom, and xylem drying and pith browning occurred at the above and below of the inoculation sites as an internal symptom. However, xylem drying began to occur 10 days after inoculation, which was determined by the difference in drying status of xylem and cortex between control and inoculated seedlings. Although population of pine wood nematode increased from 5 to 10 days after inoculation, it has increased dramatically from 10 to 20 days after inoculation when both internal and external symptoms appeared. As the time passed by after inoculation with pine wood nematodes, water content of stem and relative water content in current needles and branch gradually decreased. As the number of nematodes increases, water content of stem and relative water content of current needles and branches decreased significantly. There was a positive relationship between the number of nematodes and xylem drying and/or disease development, but the number of nematodes rapidly decreased as seedlings become severely diseased.

• Journal of Korean Society of Forest Science
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• v.55 no.1
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• pp.22-29
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• 1982

Two ectomycorrhizal fungi, Pisolithus tinctorius and Thelephora terrestris, were introduced form U.S.A. and inoculated to five pine species in Korea to evaluate the reported growth stimulation of host plants after inoculation. These fungi were grown as mycelial inoculum in large quantity and ioculated to the fumigated nursery soil just before seed sowing. At the end of the first growing season. Pisolithus stimulated the height growth of Pinus densiflora. P. thunbergii. P. rigida, and P. rigida x teada by55, 36, 69, and 37%, respectively, compared with control seedlings with no fumigation and no inoculation. When the growth stimulation was expressed with dry weight, Pisolithus increased dry weight of P. densiflora and P. rigida x taeda by 143% and 128%, respectively, over control seedling. Thelephora failed to stimulate growth of inoculated plants. Pinus koraiensis did not respond to the inoculation during the first growing season. It is concluded that artificial inoculation of nursery pine trees with selected mycorrhizal fungi should be seriously considered to improve the quality of planting stocks and to stimulate early plant growth. The potential for use of Pisolithus in reforestation on adverse sited is also discussed.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.153-153
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• 2003

Reproductive cycle with the ovarian developmental phases, first sexual maturity of the female venus clam, Cyclina sinensis, was investigated by a histological study, and the number of spawned eggs and spawning frequency by artificial spawning induction of the clams were estimated. Samples were collected from the intertidal zone of Simpo, Gimje city, Korea between January and December, 2001. The reproductive cycle of this species can be classified into five successive stages; early active (February to April), late active (March to June), ripe (May to August), spent (July to September), and spent and inactive (September to February). The spawning period was once a year between July and August, a spawning peak was seen in July and August. Percentages of first sexual maturity of female clams of 25.1-30.0 mm in shell length were 64.3%, and 100% for the clams > 40.1 mm. The number of the eggs released from each clam by spawning induction increased as the size of clam in terms of shell length increased. The mean number of the eggs released from the second induction of spawning was 76.87% of the number of the eggs released in the first spawning. Our data indicated that the interval of each spawning was estimated to be approximately 15-17 (average 16.5) days.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.189.2-190
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• 2003

현재까지 미생물의 증식을 억제하는 보존제로는 인공합성품이 많이 알려져 이들이 주로 상업적으로 사용되고 있으나 그 안정성이 경우에 따라 문제되고 있으며 근래소비자의 건강 지향적 욕구가 증대됨에 따라 인공합성품의 기피현상이 두드러지고 있다. 따라서 이들 천연 항균물질의 개발과 이용은 인공 합성보존제의 대처라는 의미와 소비자기피현상을 유발시키지 않으면서 각종 가공식품의 저장성향상 및 저온 유통식품의 안정성확보라는 견지에서 그 중요성이 있다. 본 실험에서는 식품의 미생물 작용에 의한 변질의 저해 효과가 뛰어난 식물성천연항균제품(Botanical antimicrobial agents-Citrus product : 이하 BAAC라 칭함)에 천연보조제(Ginseng extract, Aloe, 매실추출물)를 첨가하여 Paper disk 법에 의한 항균성 검사를 실시한 결과 Ginseng extract를 첨가한 경우 생육저해환이 가장 크고 뚜렷하게 나타났다. 따라서 BAAC에 보조제(Ginseng extract)를 첨가하여 제조 된 천연 항균복합제재(이하 BAAG라 칭함)를 우유에는 각 농도별로 첨가하여 4$^{\circ}C$의 냉장상태와 2$0^{\circ}C$의 상온에서 저장하였고, 계란과 콩나물의 경우는 침지 처리한 후 꺼내어 상온에서 저장하면서 각각 주사전자현미경 관찰, 대장균수, 총균수의 변화, 외관상의 변화에 미치는 영향을 무처리 대조구와 비교하면서 조사하였다. 아울러, 계란의 경우는 조단백함량의 변화도 관찰하였다. 주사전자현미경 촬영사진의 결과 분석을 통하여 BAAG를 처리한 균체 세포는 세포막 및 세포벽 기능이 파괴되어 세포내용물이 균체외부로 유출되어 균체의 생육이 억제되며 성장을 저해 또는 사멸시키는 것으로 나타났다. 대장균수는 우유, 달걀 및 콩나물의 모든 처리구에서 BAAG의 첨가에 의해 성장이 억제되었으며 농도가 증가시킬수록 균증식 억제 효과가 뚜렷하였으며, 총균수도 압도적으로 낮은 간을 보여주었다. 달걀의 조단백함량의 변화는 BAAG를 처리하지 않은 무처리 대조구에 비하여 침지 처리한 경우가 조단백 변화가 크지 않았다. 즉, BAAG의 침지 처리한 경우, 저장기간 14일이 경과 한 후에 10%정도의 수준으로 감소한데 반하여, 무처리구인 대조구의 경우, 23%수준으로 감소 증도가 증가하였다. 외관상의 변화와 상품 가치를 측정한 결과는 달걀의 경우, 14일이 경과후에는 무처리 대조구에서 흰자위의 감소와 노른자위와의 경계가 뚜렷하지 못하여 상품으로서의 가치가 크게 떨어진 상태였다. 우유의 경우에는 4$^{\circ}C$와 2$0^{\circ}C$에 저장된 대조구에서는 각각 3일과 12일경과 후 강한 부패취와 아울러, 표피의 갈변정도가 심하되어 관능적으로 부패상태를 인지할 수 있었다. 콩나물의 경우도 저장3일 경과 후에 부패취와 함께 점질성 갈변물질이 생성되었다. 이와 같은 결과로 볼 때, BAAG의 처리는 BAAC의 경우보다 가격은 저렴하면서도 항균력은 우수한 천연 항균복합제재로써 농산물 식품원료에 적용하여 선도유지 기간을 연장할 수 있는 효과를 기대할 수 있었다.

• Tuberculosis and Respiratory Diseases
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• v.52 no.5
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• pp.519-528
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• 2002

Background : Steroid therapy has been shown to improve the clinical outcome in acute respiratory distress syndrome (ARDS) patients with histological evidence of fibroproliferation in the lung tissue and no identifiable source of infection. Because the histopathological features of acute interstitial pneumonia(AIP) are identical with that of ARDS, early steroid therapy was used in AIP patients who had histological evidence of fibroproliferation in the lung tissue and no identifiable source of infection. We analyzed seven years of our experience to evaluate the efficacy of early steroid therapy in AIP. Materials and Methods : A retrospective review was performed on AIP patients who received steroid therapy within 7 days of mechanical ventilatory support in Dankook university Hospital between May 1995 and May 2002. AIP was diagnosed clinically by ARDS without a known cause of the etiology and pathologically by a lung biopsy showing a fibroproliferative stage of diffuse alveolar damage. The clinical response and physiologic parameters were evaluated during steroid therapy. Results : Five AIP patients received intravenous methylprednisolone (1-2 mg/kg every 6 hours) after $0.6{\pm}1.7$ days of mechanical ventilatory support. Lung biopsies were performed after $1.8{\pm}1.4$ days of mechanical ventilatory support. Four patients(80%) survived and were extubated after $2.8{\pm}0.4$ days of steroid therapy with improvement in the $PaO_2/FiO_2$ ratio ($127.4{\pm}10.0$ at day 0 to $223.8{\pm}37.6$ at day 7) by steroid therapy. However, one patient(20%) died of respiratory failure after 15 days of steroid therapy. Conclusion : Early steroid therapy sppears to be beneficial in AIP patients without evidence of infection. However, as our study group was too small, further large scale studies to define the effectiveness of steroids are required.