• Clinical and Experimental Reproductive Medicine
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• 제19권1호
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• pp.65-69
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• 1992

The sucess of intrauterine inseminations with washed human spermatozoa was evaluated in 92 patients. In according to indication of insemination, intrauterine inseminations of women with hostile cervical mucus yield a 35.3% and 12.8% with male factors and 27.3% with unexplained infertility. In ovulation induction group with variable agents including clomiphen, human menopausal gonadotropin (HMG) and GnRH analog, the pregnancy rate was 27.8% (22/79) and in natural cycle group, 15.4% (2/13). The fetal loss rate in insemination group was 12.5% (3/24). Multiple pregnancies were 7 cases and ovulation induction were performed in 6 cases among them. Intrauterine insemination with washed human spermatozoa therefore represents an effective and safe procedure selected infertile couples.

• 한국발생생물학회지:발생과생식
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• 제7권1호
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• pp.9-13
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• 2003

말똥성게 정자의 냉장보존을 위한 여러 가지 희석액의 보존 효과를 비교하였다. 다양한 농도의 포도당 용액, 인공해수(ASW)를 희석액으로 하여 정자를 4$^{\circ}C$에서 보존하였다 보존효과는 정자 활성지수 (SAI), 생존율 및 말똥성 게 난에 대 한 수정률로 평가하였다. ASW와 1.2 M glucose액을 희석 액으로 하여 30일간 냉장보존하였을 때 가장 높은 운동성 및 생존율을 유지하였다. 정자의 냉장보존을 위한 희석액의 적정 pH는 7.0∼8.0 이었다. 정자의 SAI와 생존율을 높이기 위한 항생제로는 neomycin(400 ppm)이 효과적인 것으로 나타났다

• Clinical and Experimental Reproductive Medicine
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• 제15권1호
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• pp.61-65
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• 1988

Retrograde ejaculation, an infrequent cause of male infertility, may be the sequala of prostate or bladder neck surgery or the result of interruption in the sympathetic innervation, the diagnosis is established by history and examination of urine. Infertile couple artificial insemination homologous(AIH) using retrograde ejaculate recovered from bladder has been successfully acomplished. In this case, ovulation was induced by clomiphene citrate, osmorality and pH of urine was controlled by buffer solution and immediately specimen collection, to improve sperm mobility. We had experienced a successful pregnancy and delivery case by above method. So here reported with brief review of literature.

• Clinical and Experimental Reproductive Medicine
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• 제15권1호
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• pp.10-16
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• 1988

Artificial insemination with donor sperm(AID) or husband sperm(AIH) has been a major form of treatment for the infertile couples with severe male factors. The conception rate in oligoasthenoteratozoospermia is very low. Therefore the aim of this study was to examine those factors associated with improved success rate. The results are obtained as the follows; 1. The husband semenalysis(n=639) revealed normal findings in 32.2%. The abnormal findings included the oligospermia(18.5%), oligoasthenospermia(20.7%), oligoasthenotetatozoospermia(8.5%) and azoospermia(20.1 %). 2. The causes of abnormal semenalysis are idiopathic(most common), varicocele, congenital anomalies, vaso-vasostomy, etc.. 3. The semen washing to improve sperm motility and concentration was effective in case of over 20 ${\times}lO^6$/ml sperm count, but there was no significant improvement in case of be low 10-5${\times}lO^6$/ml sperm count. 4. The improvement of motility after cryopreservation depended on the initial sperm concentration. 5. The pregnancy rates following AIH are higher in normospermia than oligospermia.

• 한국어류학회지
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• 제7권1호
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• pp.71-78
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• 1995

낙동강 상류의 여러 수계에서 거의 암컷으로 구성된 Cobitis sinensis - longicorpus complex 2배체와 3배체 집단이 높은 빈도로 출현하였다. 그 가운데, 수컷의 C. sinensis (2n=48) 및 C. longicorpus (2n=50)와 암컷 3배체 C. sinensis - longicorpus (3n=73)를 실험실내에서 인공 교잡 실험을 하였다. 그 결과 각 조합에서 얻은 자손의 체측 반문에는 부계 형질이 발현되었고 그들의 염색체는 모두 부계의 haploid genome이 포함된 diploid form (2n=48,49)이었다. 이상의 결과로 볼때 3배체 암컷인 Cobitis sinensis - longicorpus complex는 제 1 감수분열에서 uneven genome을 배제하고 제 2감수분열에 의한 반수체 ovum을 형성하는 독특한 생식 양상을 갖는 것으로 사료된다.

• Clinical and Experimental Reproductive Medicine
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• 제28권1호
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• pp.47-53
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• 2001

Objective: This study is to investigate the clinical efficacy of low-dose FSH regimen, comparing with clomiphene citrate and human menopausal gonadotropin (CC/hMG) regimen. Methods: Retrospective study of the ovulatory factor infertility 39 patients who had been treated by intrauterine insemination (IUI). The 31 cycles of 21 patients were stimulated by CC/hMG regimen, the 22 cycles of 18 patients were stimulated by low-dose FSH regimen. We compared the rate of clinical pregnancy, multiple pregnancy and ovarian hyperstimulation syndrome (OHSS) of both group. Results: The rate of clinical pregnancy of the CC/hMG group was 25.7% per cycle, and that of the low-dose FSH group was 54.5% per cycle. The low-dose FSH group showed a higher rate of clinical pregnancy per cycle than CC/hMG group (p=0.028). However, no differences was found statistically in the rate of multiple pregnancy and OHSS between CC/hMG group (22.2%, 5.7%) and low-dose FSH group (33.3%, 13.6%). Conclusion: This study showed that the low-dose FSH regimen is superior to CC/hMG regimen in getting clinical pregnancy, but dose not reduce the ovulation induction complications.

• 한국양식학회지
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• 제10권2호
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• pp.199-211
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• 1997

톳의 유성생식을 통한 인공종묘생산 방법을 확립하기 위하여 초기 생장에 미치는 조도, 광주기 및 온도의 효과를 연구하였다. 각기 다른 조도 (500, 1,000, 2,000, 4,000, 8,000 lux), 광주기 (8 : 16, 12 : 12, 16 : 8 (L : D))와 온도 (10, 15, 20, 25, $30^{\circ}C$) 조건에서 유배를 70일간 배양한 결과 초기 생장 단계의 최적 조건으로는 조도 2,000-4,000 lux, 광주기 16 : 8 (L : D)와 온도조건으로는 15-2$0^{\circ}C$가 효과적인 것으로 나타났다.

• Clinical and Experimental Reproductive Medicine
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• 제20권1호
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• pp.57-63
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• 1993

From September 1988 to August 1992, two different methods of preparing human sperm before intrauterine insemination(IUI) were compared using the semen samples of seventy-three infertile couples. The sperms were prepared by a swim-up after sperm washing or by a continuous percoll gradient technique. Fourteen of 35 women conceived during IUI cycles using a sperm washing and swim-up method (40%), and 12 of 38 women conceived during IUI cycles using a percoll gradient technique(31.6%). Among the group with male infertile etiologic factor only, one of 5 women conceived during sperm washing and swim-up cycles(20%); one of 4 women conceived during percoll gradient cycles(25%). On the contrary, among the group with cervical factor only, six of 10 women conceived during sperm washing and swim-up cycles (60%) ; Five of 17 women conceived during percoll gradient cycles(29.4%). It is suggested that sperm separation by sperm washing ar -up is a useful technique for intrauterine insemination in cervical infertility, and sperm separation in percoll gradient appears to be more valuable for intrauterine insemination of male subfertility.

• 대한수의학회지
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• 제38권1호
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• pp.77-83
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• 1998

돼지 생식기호흡기증후군(porcine reproductive and respiratory syndrome, PRRS) 바이러스가 웅돈에 감염되었을 경우에는 정충의 기형 등 정액의 질 저하와 더불어 정액에 바이러스가 함유되어 있어 종부시 모돈에 바이러스를 전파하는 것으로 알려져 있다. 감염된 웅돈의 정액으로 인공수정을 실시할 경우 농장의 모돈 전체에 순식간에 바이러스를 전파하여 막대한 피해를 유발할 가능성이 높다. 따라서 감염웅돈을 신속히 검색하여 격리함으로써 피해를 사전에 방지해야 하며, 이를 위해서 웅돈의 감염여부를 신속히 확진하는 진단법의 개발이 필요한 실정이다. PRRS의 진단을 위해서는 바이러스학적인 진단법으로는 바이러스 분리동정, 혈청학적인 방법으로 바이러스 분리동정이 필수적이나 검사시간이 많이 소요되고, 분리동정 자체가 까다로운 단점이 있다. 본 연구에서는 웅돈의 정액내에서 PRRSV 바이러스에 대한 유전자를 RT-PCR법으로 증폭하는 방법을 개발하였으며, 진단의 민감도를 높이기 위하여 Nested PCR법으로 재확인 할 경우, 바이러스의 역가가 $1TCID_{50}$만 함유되어 있어도 진단이 가능한 조건을 확립하였다. 이 방법을 이용할 경우 웅돈의 정액시료에 대한 PRRS 바이러스 감염여부를 신속, 정확하게 검색하여 감염웅돈을 통한 PRRS바이러스의 전파를 미리 차단할 수 있으므로 PRRS방제에 효과적으로 이용될 것으로 사료된다.

• Clinical and Experimental Reproductive Medicine
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• 제17권2호
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• pp.137-144
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• 1990

Seventy-two patients initiated intrauterine insemination between March 1989 and March 1990. Indication for the intrauterine insemination included abnormal semen, poor cervical mucus, presents of sperm antibody, unexplained, poor postcoital test and endometriosis. During this time period, 216 inseminations were performed and resulted in twenty four pregnancies in the 72 patients receiving artificial insemination by homologous donor for an overall pregnancy rate of 33.3%. Where pergonal treatment was followed by insemination, a significantly greater pregnancy per cycle was achieved. It is therefore suggested that a trial if induction of multiple follicular development be performed on coulpes with male-related or unexplained infertility before inclusion in protocols for invasive procedures, such as IVF or GIFT.