• Title/Summary/Keyword: 이완시간

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$In$ $vitro$ MRI and Characterization of Rat Mesenchymal Stem Cells Transduced with Ferritin as MR Reporter Gene (페리틴 리포터 유전자를 발현하는 백서 중간엽 줄기세포의 특성과 자기공명영상 연구)

  • Shin, Cheong-Il;Lee, Whal;Woo, Ji-Su;Park, Eun-Ah;Kim, Pan-Ki;Song, Hyun-Bok;Kim, Hoe-Suk
    • Investigative Magnetic Resonance Imaging
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    • v.16 no.1
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    • pp.47-54
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    • 2012
  • Purpose : This study was performed to evaluate the characteristics of rat mesenchymal stem cells (RMSCs) transduced with human ferritin gene and investigate $in$ $vitro$ MRI detectability of ferritin-transduced RMSCs. Materials and Methods: The RMSCs expressing both myc-tagged human ferritin heavy chain subunit (myc-FTH) and green fluorescence protein (GFP) were transduced with lentiviurs. Transduced cells were sorted by GFP expression using a fluorescence-activated cell sorter. Myc-FTH and GFP expression in transduced cells were detected by immunofluorescence staining. The cell proliferative ability and viability were assessed by MTT assay. The RMSC surface markers (CD29+/CD45-) were analyzed by flow cytometry. The intracellular iron amount was measured spectrophotometically and the presence of ferritin-iron accumulation was detected by Prussian blue staining. $In$ $vitro$ magnetic resonance imaging (MRI) study of cell phantoms was done on 9.4 T MR scanner to evaluate the feasibility of imaging the ferritin-transduced RMSCs. Results: The myc-FTH and GFP genes were stably transduced into RMSCs. No significant differences were observed in terms of biologic properties in transduced RMSCs compared with non-transduced RMSCs. Ferritin-transduced RMSCs exhibited increased iron accumulation ability and showed significantly lower $T_2$ relaxation time than non-transduced RMSCs. Conclusion: Ferritin gene as MR reporter gene could be used for non-invasive tracking and visualization of therapeutic mesenchymal stem cells by MRI.

Pullout Characteristics of Pressure Reinjection-Grouted Reinforcements in Decomposed Granite Soil (화강풍화토 지반에 설치된 압력재주입 그라우팅 보강재의 인발특성)

  • Shim, Yong-Jin;Lee, Jong-Kyu;Lee, Bong-Jik
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.11
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    • pp.61-68
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    • 2012
  • Most widely methods for reinforcement of soil utilized in Korea are anchor method, soil nail method and micro pile method. These methods are classified by the intended use of the structure to be constructed, but the reinforcement of the ground is accomplished contains in common the process of grouting work after inserting the reinforcements. Domestically, gravity grouting has been used mostly so far, but there has always been the risk of insufficient restoration of the loose ground area from the drill holes because the grouting is conducted only by gravity. On the other hand, pressure reinjection grouting may enhance the grouting quality by solving the problem of the existing grouting method considerably since it additionally reinjects grouting through pre-installed tube a certain time after the first grouting. Accordingly, this study evaluated the pullout characteristics by the grouting methods by performing model test on decomposed granite soil, and investigated the support increasing characteristics of reinforcements depending on the curing time, reinjection pressure, and uplift force variation of the pressure reinjection grouting. The result of this research shows that the pressure reinjection grouting demonstrated 1.1~1.3 times of performance of the gravity grouting, and suggests some analysis on optimal water content, reinjection pressure and curing time of the pressure reinjection grouting.

A Study on the Warpage of Glass Fiber Reinforced Plastics for Part Design and Operation Condition: Part 2. Crystalline Plastics (유리섬유로 보강된 수지에서 제품설계 및 성형조건에 따른 휨의 연구: Part 2. 결정성 수지)

  • Lee, Min;Kim, Hyeok;Lyu, Min-Young
    • Polymer(Korea)
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    • v.36 no.6
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    • pp.677-684
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    • 2012
  • Injection molding process is a popular polymer processing involving plasticizing and enforcing the material flow into the mold. A polymer material shrinks according to temperature variations during the shaping process, and subsequently molding shrinkage developed. Developed deflections or warpages after molding process in part are caused by residual stress relaxation contained in the part. Adding inorganic materials or fibers such as glass and carbon to control shrinkage and enhance warpage resistance are common. In this study, warpages according to part design have been investigated through experiment. Warpages for molding conditions and mold designs such as gate locations were measured. Warpages along flow direction and perpendicular to the flow direction were also measured. Warpages near gate and far from gate were compared. Glass fiber reinforced crystalline polymers, PP and PA66 have been used in this experiment. Glass fiber reinforced crystalline polymers showed large warpage compared with glass reinforced amorphous polymers. Warpages in crystalline polymers were less influenced by molding conditions compared with amorphous polymers, however warpages of crystalline polymers significantly depend on part design.

Inhibitory Effect of Chlorine Dioxide Using Reactive Oxygen Species Against Heart Contraction of the Indianmeal Moth, Plodia interpunctella (이산화염소의 활성산소 발생에 따른 화랑곡나방 심장박동 억제 효과)

  • Kim, Chul-young;Kwon, Hyeok;Kim, Wook;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.56 no.2
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    • pp.147-152
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    • 2017
  • Heart contraction is essential for insect hemolymph circulation, in which various physiological changes need control of the heart contraction. Thus, interruption of heartbeats intimidate insect survival. Chlorine dioxide induces release of reactive oxygen species (ROS), which has been regarded as the main insecticidal agent. This study analyzed the effect of chlorine dioxide on heartbeats of the Indianmeal moth, Plodia interpunctella. The larvae have the dorsal vessel on the medial dorsal region in a form of an extending tube from the first thorax to 10th abdominal segment. Dorsal vessel contraction was noticeable between 3rd and 10th abdominal segments, where five heart chambers were observed. Average heartbeat rate was 118.6 beats per min at $25^{\circ}C$. However, the heartbeats varied with ambient temperature. Injection of chlorine dioxide to hemocoel suppressed the heartbeats in a dose-dependent manner. The suppressive effect of chlorine dioxide also increased with exposure time to gas form of the chemical at 100 ppm. However, vitamin E (an antioxidant against ROS) treatment significantly rescued the larvae treated with chlorine dioxide in the heart contraction activity. These results suggest that chlorine dioxide negatively influences on the heart contraction of P. interpunctella via its production of ROS.

Survey Study on Radioactivity of Domestic Fishery Product (국내 시중 유통 수산물에 대한 방사능 농도 조사)

  • Kim, Chang-Jong;Lim, Chung-seop;Lee, Wanno;Jang, Mee;Ji, Young-Yong;Chung, Kun-Ho;Kang, and Mun-Ja
    • Korean Journal of Food Science and Technology
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    • v.47 no.6
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    • pp.789-792
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    • 2015
  • Samples of fishery products were tested for radioactivity by using the intake frequency data from Korea Health Statistics. The radioactivity of $^{40}K$, $^{137}Cs$, $^{134}Cs$, and $^{131}I$ was analyzed using gamma spectrometry with a simplified sample pre-treatment procedure. The radioactivity range for $^{40}K$ was 21.9-3050 Bq/kg, whereas the radioactivities of $^{137}Cs$, $^{134}Cs$, and $^{131}I$ were under minimum detectable activity which were in the range of 0.140-1.97, 0.0900-1.89 and 0.124-1.94 Bq/kg, respectively, for the three species. The results suggest that the Fukushima accident did not have a significant impact on domestic fishery products, which were analyzed during the period from 2013 to 2015. Additionally, there seemed to be no significant impact of additional exposure dose by the analyzed radionuclides.

CVD Growth of Grapbene on a Thin Ni Film (Ni 금속 박막위 그라핀 CVD 성장 연구)

  • Choi, In-Sung;Kim, Eun-Ho;Park, Jae-Min;Lee, Han-Sung;Lee, Wan-Kyu;Oh, Se-Man;Cho, Won-Ju;Jung, Jong-Wan;Lee, Nae-Sung
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.06a
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    • pp.425-425
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    • 2009
  • 그라핀을 금속 촉매를 이용하여 상압 혹은 저진공 CVD로 성장할 경우 대형 기판을 쉽게 얻을 수 있으므로 최근 들어 금속 촉매를 이용한 CVD 기술이 재 각광받고 있다. 최근 MIT의 Jing Kong 그룹, Purdue 대학의 Yong P. Chen 그룹, 국내에서는 성균관대학에서 이에 대한 논문을 발표한 바 있다. CVD 방법의 가장 큰 장점은 그라핀 박막의 가장 큰 문제점 중 하나인 대형 기판에 매우 유리하다는 점이다. 본 연구에서는 결함 없는 대형 그라핀기판을 얻기위해 Si/$SiO_2$/Ni 박막위에 그라핀을 LPCVD로 성장하는 실험을 진행하였다. 우선 시료는 Si위에 $SiO_2$를 Sputtering으로 증착하였고, 그 위에 250nm, 300nm두께의 Ni 박막을 e-beam evaporator로 증착하였다. $0.5-1cm^2$ 크기의 샘플을 Thermal CVD 장비를 이용하여 그라핀을 성장하는 실험을 진행하였다. 성장 압력은 95 torr, 성장온도는 $800^{\circ}C$, $850^{\circ}C$, $900^{\circ}C$에서 Hydrocarbon ($C_2H_2$)을 5min, 10min으로 성장시간을 split하였다. Hydrocarbon을 흘리기 전에 Ni grain을 성장하기 위해 성장온도에서 30~60min정도 $H_2$분위기에서 Ni 산화막의 환원 및 어닐링을 진행하였다. 그림.1은 $850^{\circ}C$, 5분간 성장한 그라핀/Ni 샘플의 광학사진이다. 그림.2는 $850^{\circ}C$에서 5min, 10min 성장한 샘플의 Raman spectrum이다. (파장은 514.532nm). 850C 10min 샘플은 G>G' peak 이지만, 5min으로 성장한 샘플의 경우 G'>G peak 임을 알 수 있고, 따라서 5min의 조건에서는 층 두께가 4층 미만의 그라핀 박막을 얻을 수 있음을 보여준다. 또한 G' peak의 위치가 두께가 감소할수록 내려감을 확인할 수 있다. 다만 D peak가 실험한 대부분의 샘플에서 보여서 아직 성장한 그라핀의 결합이 많은 것으로 보인다. 이러한 이유는 성장온도가 낮은 것이 일차 원인으로 생각되며 박막의 균일도 향상과 결함을 줄이기 위한 추가적인 개선 실험을 진행 중이다.

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Real-time Environmental Radiation Monitoring System with Automatic Restoration of Backup Data in Site Detector via Communication using Radio Frequency (현장검출기에 저장된 백업데이터를 무선통신방식으로 자동 복원하는 실시간 환경선량 감시 시스템)

  • Lee, Wan-No;Kim, Eun-Han;Chung, Kun-Ho;Cho, Young-Hyun;Choi, Geun-Sik;Lee, Chang-Woo;Park, Ki-Hyun;Kim, Yun-Goo
    • Journal of Radiation Protection and Research
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    • v.28 no.3
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    • pp.255-261
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    • 2003
  • An environmental radiation monitoring system based on high pressurized ionization chamber has been used for on-line gamma monitoring surrounding the KAERI (Korea Atomic Energy Research Institute), which transmits the dose data measured from ion chamber on the site via radio frequency to a central processing computer and stores the transmitted real-time data. Although communication using radio frequency has several advantages such as effective and economical transmission, storage, and data process, there is one main disadvantage that data loss during transmission often happens because of unexpected communication problems. It is possible to restore the loss data by off-line such as floppy disk but the simultaneous process and display of current data as well as the backup data are very difficult in the present on-line system. In this work, a new electronic circuit board and the operation software applicable to the conventional environmental radiation monitoring system are developed and the automatical synchronization of the ion chamber unit and the central processing computer is carried out every day. This system is automatically able to restore the backup data within 34 hours without additional equipments and also display together the current data as well as the transmitted backup data after checking time flag.

Study on Evaluation Factors Determining Satisfaction and Revisiting of Broadcasting Contents Market (방송콘텐츠마켓 만족도와 재방문에 미치는 평가요인: 부산콘텐츠마켓(BCM) 사례를 중심으로)

  • Gu, Jong-Sang;Lee, Wan-Soo;Lee, Jin-Ro
    • Korean journal of communication and information
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    • v.52
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    • pp.111-134
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    • 2010
  • This paper explored satisfaction and revisiting of participants on Busan Contents Market(BCM) programme in 2009. Specifically, this study conducted regression analysis for finding satisfaction and revisiting factors on main events, broadcasting-telecom convergence forum, broadcasting business marketing forum, world culture contents forum and BCM academy. Results show as following. Firstly, Participants satisfied on comfortable lodging and possibilities as international events, but not satisfied on facilities, managements, public relations, contents and service. In particular, regional attraction and interpretation service are one of factors for revisiting in BCM main events. Secondly, one of competition factors for revisiting intention in BCM main events is production and investment. Thirdly, contents, theme and qualities of presenters and discussants are main factors for revisiting in BCM. Fourthly, satisfaction degree on education contents and lectures in BCM academy is high and contents, time, line up of lectures analyzed as determined factor for revisiting. Fifthly, documentary planning, production and marketing with international investors drive to induce revisiting BCM. We discussed motivation and utility values for revisiting of broadcasting contents market.

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Automatic Left Ventricle Segmentation Algorithm using K-mean Clustering and Graph Searching on Cardiac MRI (K-평균 클러스터링과 그래프 탐색을 통한 심장 자기공명영상의 좌심실 자동분할 알고리즘)

  • Jo, Hyun-Wu;Lee, Hae-Yeoun
    • The KIPS Transactions:PartB
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    • v.18B no.2
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    • pp.57-66
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    • 2011
  • To prevent cardiac diseases, quantifying cardiac function is important in routine clinical practice by analyzing blood volume and ejection fraction. These works have been manually performed and hence it requires computational costs and varies depending on the operator. In this paper, an automatic left ventricle segmentation algorithm is presented to segment left ventricle on cardiac magnetic resonance images. After coil sensitivity of MRI images is compensated, a K-mean clustering scheme is applied to segment blood area. A graph searching scheme is employed to correct the segmentation error from coil distortions and noises. Using cardiac MRI images from 38 subjects, the presented algorithm is performed to calculate blood volume and ejection fraction and compared with those of manual contouring by experts and GE MASS software. Based on the results, the presented algorithm achieves the average accuracy of 6.2mL${\pm}$5.6, 2.9mL${\pm}$3.0 and 2.1%${\pm}$1.5 in diastolic phase, systolic phase and ejection fraction, respectively. Moreover, the presented algorithm minimizes user intervention rates which was critical to automatize algorithms in previous researches.

Optical spectroscopy of Tb3+ ions doped NaCa(PO3)3 phosphors (Tb3+ 이온이 첨가된 NaCa(PO3)3 형광체의 형광특성)

  • Yoon, Changyong
    • Journal of the Korean Society of Radiology
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    • v.12 no.4
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    • pp.451-457
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    • 2018
  • Luminescence properties of $NaCa(PO_3)_3$ doped with $Tb^{3+}$ ions are investigated by optical and laser excitation spectroscopy. The phosphors were prepared by solidstate reaction method The X-ray diffraction(XRD) was used to analyze the crystal structure and the crystallinity of the samples. The excitation and emission spectra and decay curve of $NaCa(PO_3)_3:Tb^{3+}$(0.01 ~ 30mol%) were measured at room temperature. The f - d band of $Tb^{3+}$ is observed in the excitation spectra of $NaCa(PO_3)_3:Tb^{3+}$ in the wave length region 205 ~ 245 nm. Strong emission lines due to the $^5D_4{\rightarrow}^7F_J$ transition and weak emission lines due to the $^5D_4{\rightarrow}^7F_J$ transition are observed in the emission spectra of $NaCa(PO_3)_3:Tb^{3+}$. The energy transfer and cross relaxation between $Tb^{3+}$ ions are discussed in $NaCa(PO_3)_3:Tb^{3+}$ in the emission spectra and life time.