Browse > Article
http://dx.doi.org/10.5656/KSAE.2017.03.0.007

Inhibitory Effect of Chlorine Dioxide Using Reactive Oxygen Species Against Heart Contraction of the Indianmeal Moth, Plodia interpunctella  

Kim, Chul-young (Department of Plant Medicals, Andong National University)
Kwon, Hyeok (Department of Biosystems and Biotechnology, Korea University)
Kim, Wook (Department of Biosystems and Biotechnology, Korea University)
Kim, Yonggyun (Department of Plant Medicals, Andong National University)
Publication Information
Korean journal of applied entomology / v.56, no.2, 2017 , pp. 147-152 More about this Journal
Abstract
Heart contraction is essential for insect hemolymph circulation, in which various physiological changes need control of the heart contraction. Thus, interruption of heartbeats intimidate insect survival. Chlorine dioxide induces release of reactive oxygen species (ROS), which has been regarded as the main insecticidal agent. This study analyzed the effect of chlorine dioxide on heartbeats of the Indianmeal moth, Plodia interpunctella. The larvae have the dorsal vessel on the medial dorsal region in a form of an extending tube from the first thorax to 10th abdominal segment. Dorsal vessel contraction was noticeable between 3rd and 10th abdominal segments, where five heart chambers were observed. Average heartbeat rate was 118.6 beats per min at $25^{\circ}C$. However, the heartbeats varied with ambient temperature. Injection of chlorine dioxide to hemocoel suppressed the heartbeats in a dose-dependent manner. The suppressive effect of chlorine dioxide also increased with exposure time to gas form of the chemical at 100 ppm. However, vitamin E (an antioxidant against ROS) treatment significantly rescued the larvae treated with chlorine dioxide in the heart contraction activity. These results suggest that chlorine dioxide negatively influences on the heart contraction of P. interpunctella via its production of ROS.
Keywords
Chlorine dioxide; Heartbeat; Reactive oxygen species; Plodia interpunctella;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Brigelius-Flohé, R., Traber, M.G., 1999. Vitamin E: function and metabolism. FASEB J. 13, 1145-1155.   DOI
2 Chiang, R.G., Chiang, J.A., Davey, K.G., 1990. Morphology of the dorsal vessel in the abdomen of the blood-feeding insect Rhodnius prolixus. J. Morphol. 204, 9-23.   DOI
3 Gibbs, S.G., Lowe, J.J., Smith, P.A., Hewlett, A.L., 2012. Gaseous chlorine dioxide as an alternative for bedbug control. Infect. Control Hosp. Epidemiol. 33, 495-499.   DOI
4 Hamoudi, Z., Lange, A.B., Orchard, I., 2016. Identification and characterization of the corazonin receptor and possible physiological roles of the corazonin-signaling pathway in Rhodnius prolixus. Front. Neurosci. 10, 357.
5 Hertel, W., Pass, G., 2002. An evolutionary treatment of the morphology and physiology of circulatory organs in insects. Comp. Biochem. Physiol. A 133, 555-575.   DOI
6 Kim, Y., Kumar, S., Rahman, M.M., Kwon, H., Chon, Y., Na, J., Kim, W., 2015a. Evasive behavior of the red flour beetle, Tribolium castaneum, against chlorine dioxide and its suppression by heat treatment. Korean J. Appl. Entomol. 554, 151-158.
7 Kim, Y., Park, J., Kumar, S., Kwon, H., Na, J., Chun, Y., Kim, W., 2015b. Insecticidal activity of chlorine dioxide gas by inducing an oxidative stress to the red flour beetle, Tribolium castaneum. J. Stored Prod. Res. 64, 88-96.   DOI
8 Kumar, S., Park, J., Kim, E., Na, J., Chun, Y.S., Kwon, H., Kim, W., Kim, Y., 2015. Oxidative stress induced by chlorine dioxide as an insecticidal factor to the Indian meal moth, Plodia Interpunctella. Pesti. Biochem. Physiol. 124, 48-59.   DOI
9 Miller, T.A., 1974. Electrophysiology of the insect heart. In: Rockstein, M. (Ed.), Physiology of the Insecta, 2nd Edn. Vol. V, pp. 169-200. Academic Press, New York.
10 Miller, T.A., 1997. Control of circulation in insects. Gen. Pharmacol. 29, 23-38.   DOI
11 Sanz, A., 2016. Mitochondrial reactive oxygen species: Do they extend or shorten animal lifespan? Biochim. Biophys. Acta 1857, 1116-1126.   DOI
12 SAS Institute, Inc., 1989. SAS/STAT user's guide. SAS Institute, Inc., Cary, NC.
13 Young, R.O., 2016. Chlorine dioxide ($ClO_{2}$) as a non-toxic antimicrobial agent for virus, bacteria and yeast (Cadida albicans). Int. J. Vaccines Vaccin. 2, 00052.
14 Shim, J.K., Ha, D.M., Nho, S.K., Song, K.S., Lee, K.Y., 2008. Upregulation of heat shock protein genes by envenomation of ectoparasitoid Bracon hebetor in larval host of Indian meal moth Plodia interpunctella. J. Invertebr. Pathol. 97, 306-309.   DOI
15 Slama, K., Sakai, T., Taneda, M., 2006. Effect of corazonin and crustacean cardioactive peptide on heartbeat in the adult American cockroach (Periplaneta americana). Arch. Insect Biochem. Physiol. 62, 91-103.   DOI
16 Smits, A.W., Burggren, W.W., Oliveras, D., 2000. Developmental changes in in vivo cardiac performance in the moth Manduca sexta. J. Exp. Biol. 203, 369-378.
17 Tartes, U., Vanatoa, A., Kuusik, A., 2002. The insect abdomen--a heartbeat manager in insects? Comp. Biochem. Physiol. A 133, 611-623.   DOI
18 Tublitz, N.J., Truman, J.W., 1983. Insect cardioactive peptides. II. Neurohormonal control of heart activity by two cardioacceleratory peptides in the tobacco hawkmoth, Manduca sexta. J. Exp. Biol. 114, 381-395.