• Journal of the Korean Chemical Society
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• v.30 no.2
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• pp.224-230
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• 1986

Dodecyltrimethylammonium bromide (DTAB) was examined as a counter-ion in the separation of aromatic sulfonic acids on alkylmodified silica (ODS) column by reversed-phase ion-pair chromatography. The capacity factor of acids was investigated and separation was attempted. The capacity factor of samples was influenced by the several factors such as a concentration of counter-ion, concentration of methanol, kinds and concentration of electrolyte in mobile phase as well as kinds and position of functional group in samples. Some mixtures of samples were able to be separated under optimum condition.

• Proceedings of the Membrane Society of Korea Conference
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• 1991.10a
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• pp.45-46
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• 1991

분리막 공정은 상변화를 일으키지 않기 때문에 장치비가 저렴하며, 열 및 화학적으로 민감한 물질, 특히 생체물질 등을 다루는데 적합하다. 또한 최종 생성물질을 정제하는 방법으로는 이온교환 크로마토그래피, 전기 영동 및 친화성 크로마토그래피(affinity chromatography)가 있다. 이중 친화성 크로마토그래피는 가장 선택적이고, 단일 장치로서 기존의 분리방법에 비해 약 1000배 가까이 정제할 수 있다. 본 실험에서는 트립신 억제제인 m-aminobenzamidine을 포함하고 있는 수용성 고분자를 제조하여 트립신을 선택적으로 분리하고자 하였으며, 본 연구 필요한 분리막은 막제조가 용이하며 비용이 저렴한 cellulose acetate막을 사용하였다. CA 막의 세공크기는 겔화매질의 에탄올 농도로 조절하였다. Casting 용액의 조성은 다음 표에 간략하게 나타내었다.

• Membrane Journal
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• v.34 no.2
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• pp.124-131
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• 2024

Viruses have various applications in the biopharmaceutical industry. They are used in pesticide production, production of vaccines, gene transfers, cancer therapeutics, and more. The downstream processing of viruses is an essential step for their biological and pharmaceutical applications. Among the various processes, the purification of viruses is critical. Membrane chromatography plays a vital role in this process. While ion exchange membrane chromatography is a primarily used method, it has various limitations regarding size exclusion and insufficient purification. Also, it cannot be applied to the rapidly changing strains of viruses such as influenza. This review examines various improved methods of membrane chromatography or alternatives. It focuses on purification, viral recovery rates, and scalability of the methods.

• Proceedings of the Korea Water Resources Association Conference
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• 1995.05a
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• pp.197-203
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• 1995

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2003.11a
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• pp.366-367
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• 2003

암모니아 가스는 무색의 기체이지만 자극성이 크면서 부식성이 있고 수용액이 알칼리성인 대표적인 악취물질이다. 보통의 경우는 굴뚝 등에서 배출가스의 형태로 대량 방출되기도 하지만 자연상태에서도 여러 가지 생물학적 또는 화학적 반응 등을 통하여 생성되므로, 일상적인 생활 공간에서도 쉽게 검출될 수 있는 물질이다. 대기 중 암모니아의 농도는 기체상 시료를 직접 분석하는 가스 크로마토그래피와 시료를 액체상으로 변환시킨 후 암모늄 양이온의 농도를 분광광도법이나 중화적정법으로 측정한다. (중략)

• Journal of the Korean Chemical Society
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• v.30 no.3
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• pp.312-319
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• 1986

Dodecyltrimethylammonium bromide (DTAB) was examined as a counter-ion in the investigation of capacity factor and separation of aromatic carboxylic acids on alkyl-modified silica (ODS) column as a stationary phase by ion-pair chromatography on reversed-phase system. The capacity factor of samples was influenced by the several factors such as a concentration of counter-ion as well as kinds and concentration of electrolyte, concentration of methanol in mobile phase and kinds and position of functional group in sample molecule. Some mixtures of samples were able to be separated under optimum condition.

• Journal of the Korean Chemical Society
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• v.34 no.1
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• pp.69-75
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• 1990

An ion-association chromatography was applied for the separation and determination of individual rare earth elements (REE) contained in mineral monazite. Prior to the determination, the group separation of REE was achieved by a cation exchange column of Dowex 5OW-X8 resin. The quantitative recovery of REE by the resin column, free from coexisting elements in monazite, was confirmed with radioactive tracers as well as with ICP-MS. Individual REE at ppm level was separated on reversed-phase column ($\mu$-Bondapak $C_{18}$) using gradient elution from 0.05 to 0.3 M $\alpha$-hydroxyisobutyric acid at pH 4.6. The individual REE was detected at 546 nm following post-column reaction with PAR (4-(2-pyridylazo)-resorcinol monosodium salt).

• Analytical Science and Technology
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• v.7 no.2
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• pp.201-204
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• 1994

Cation exchange column chromatography of lithium was carried out to investigate the lithium isotope separation in aqueous ion exchange system. A Pyrex glass column of $50cm{\times}6mm$ inner radius with a water jacket was used as the separation column in experiment. Upon column chromatography using hydrochloric and succinic acid mixtures as an elunent, single separation factor, ${\alpha}$, 1.0068 was obtained. From the experiment, it was found that $^6Li$ was enriched in the resin phase and $^7Li$ in the solution phase.

• Journal of the Korean Chemical Society
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• v.34 no.1
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• pp.44-50
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• 1990

The liquid chromatographic retention behavior of structural isomers of phenols was investigated by a change of the mobile phase properties. The retention behavior of structural isomer of phenols in reversed phase liquid chromatography was affected by eluent electrolyte added. It can be seen that this behavior is illustrated by a mechanism of Langmuir isotherm and ion exchange between phenolate and the reversed phase coated with ions. The retention behavior was represented as two different areas according to the concentration of the electrolytes. These areas can be explained as counter ion and co-ion effect, respectively. The maximum retention values were dependent not upon the kinds of organic modifier but upon the kinds of electrolyte.

• Membrane Journal
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• v.31 no.2
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• pp.145-152
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• 2021

While there are increasing demands on biomolecules separation, resin chromatography lacks in terms of throughput and membrane chromatography is an alternative with high binding capacity and enhanced mass transfer properties. Unlike typical membrane processing, where the performance can only be empirically assessed, understanding how mechanisms work in membrane chromatography is decisive to design biospecific processing. This short review covers three separation mechanisms, including affinity interaction modes for selectively capturing bulk molecules using biospecific sites, ion exchange modes for binding biomolecules using net charges and hydrophobic interaction modes for binding targeted, hydrophobic species. The parameters in designing membrane chromatography that should be considered operation-wise or material-wise, are also further detailed in this paper.