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The Membrane Society of Korea (한국막학회)
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How to Design Membrane Chromatography for Bioseparations: A Short Review

바이오분야 분리용 막크로마토그래피 설계 방안

  • Park, Inho (Department of Energy Engineering, Hanyang University) ;
  • Yoo, Seung Yeon (Department of Energy Engineering, Hanyang University) ;
  • Park, Ho Bum (Department of Energy Engineering, Hanyang University)
  • 박인호 (한양대학교 에너지공학과) ;
  • 유승연 (한양대학교 에너지공학과) ;
  • 박호범 (한양대학교 에너지공학과)
  • Received : 2021.04.22
  • Accepted : 2021.04.28
  • Published : 2021.04.30
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Abstract

While there are increasing demands on biomolecules separation, resin chromatography lacks in terms of throughput and membrane chromatography is an alternative with high binding capacity and enhanced mass transfer properties. Unlike typical membrane processing, where the performance can only be empirically assessed, understanding how mechanisms work in membrane chromatography is decisive to design biospecific processing. This short review covers three separation mechanisms, including affinity interaction modes for selectively capturing bulk molecules using biospecific sites, ion exchange modes for binding biomolecules using net charges and hydrophobic interaction modes for binding targeted, hydrophobic species. The parameters in designing membrane chromatography that should be considered operation-wise or material-wise, are also further detailed in this paper.

현재 바이오 분야에서 분리에 대한 수요가 급증함에 따라, 투과율 및 결합능 측면에서 높은 성능을 띠는 막크로마토그래피가 수지 크로마토그래피의 대체 분리 공정으로 부상하고 있다. 실증을 기반으로 하여 막 소재가 결정되는 기존 분리막 공정과 달리, 막크로마토그래피의 경우 분리하고자 하는 목표 물질에 적합한 분리 메커니즘 이해 그리고 이를 기반한 공정 설계가 필요하다. 본 논문에서는 생특이성을 활용하여 선택적으로 거대 분자를 포집하는 친화성 작용, 전하를 활용하여 생분자와 결합하는 이온 교환 작용 그리고 소수성을 활용하여 생분자와 결합하는 소수성 작용과 같은 막크로마토그래피 주요 분리 메커니즘들에 대해 다루고자 한다. 또한 본 논문에서는 단계적 측면에서 또는 소재 측면에 막크로마토그래피 기술설계 시 고려해야할 변인들에 대해서 다루고자 한다.

Keywords

Acknowledgement

This work was supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (No. 20202020800330).

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