• Analytical Science and Technology
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• v.19 no.5
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• pp.383-388
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• 2006

Simple post-wash method by ion chromatography (IC) was established for the rapid and precise determination of fluoride ion in wastewater from mine in fluorite mineralized area. High sulfate in sample was retained in a pre-column and less strongly held fluoride ion was transferred to the principal separation system using modified conventional IC with switching technique. An analytical column with high capacity (AS 9 HC) was used as a pre-column to retain the amount of high sulfate. A guard column (AG 14) as a separation column was used to increase the response of fluoride and reduce the system pressure. According to the recovery of fluoride ion with one detector and the observation of sulfate peak with another conductivity detector, the optimum switching time of 10-port chromatographic injector was 4.3 min. The limit of detection (S/N = 3) of fluoride in synthetic solution containing $500mg\;L^{-1}$ sulfate was $2.4{\mu}g/L$, with $25{\mu}L$ sample volume.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.60-60
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• 1993

새로운 항생물질을 개발하기 위하여 토양으로부터 분리한 균주를 액체배지에서 배양하여 그 배양액을 여러 검정균에 대하여 종이디스크법으로 항균효력을 측정하였다. 그 결과 G(＋), G(－)균에 강한 항균효력을 보인 토양균 SNUS 9101-55와 C. aibicans와 같은 진균류에 항균력을 보인 토양균 SN-US 9101-68을 선택하여 각각의 배양액에서 항생물질을 분리하고, 분리한 항생물질의 구조를 규명하고자 하였다. 토양균 SNUS 9101-55의 배양액으로부터 항생물질을 분리하기 위하여 양이온 교환수지 관 크로마토그래피와 셀룰로오스 관 크로마토그래피를 수행하여 시료 AMJ-I-212-B를 얻었다. 시료 AMJ-I-212-B의 IR, $^1$H-NMR, FAB-MS 스펙트럼을 얻어 분리한 항생물질의 구조를 분석하고 Sakaguchi test와 같은 정색반응을 통하여 그 발색단을 동정하여 이 항생물질의 구조가 스트렙토마이신과 동일하다는 것을 확인하였다.

• Journal of the Korean Chemical Society
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• v.6 no.1
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• pp.84-87
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• 1962

A new peptide was isolated from an edible brown algae "Undaria pinnatifida" by both two dimensional and ion-exchange resin chromatographies. The peptide was composed of three amino acids, glutamic acid, alanine and aspartic acid.

• Analytical Science and Technology
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• v.12 no.2
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• pp.111-115
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• 1999

Single column anion chromatography by indirect UV detection was applied to the determination of 7 common anions($H_2PO_4^-$, $Cl^-$, $NO_2^-$, $NO_3^-$, $I^-$, $SO_4^{2-}$, $SCN^-$). A low capacity silica-based anion-exchange column(TSK guardgel QAE-SW; ca. 0.3 meq./g; 4.6 mm i.d${\times}$150 mm) was used. In separating common anions, we compared naphthalene trisulfonic acid(NTS) and naphthalene disulfonic acid(NDS) to find more sensitive, selective and rapid separation method. And cations and anions can be separated simultaneously in 0.20 mM NTS-5% acetonitrile-water.

• Analytical Science and Technology
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• v.14 no.1
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• pp.28-33
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• 2001

The ion chromatography for the separation of plutonium species which are suggested to be $Pu^{3+}$, $Pu^{4+}$, $PuO_2{^+}$ and $PuO_2{^{2+}}$ in natural water was studied. Two separation methods were performed; 1) two-column method containing each of $SiO^-$ and SiO-$SO_3{^-}$ cation exchanger, 2) IC with AG11 column and the eluent of oxalate/nitric acid. Separation conditions for $Eu^{3+}$, $Th^{4+}$, $NpO_2{^+}$, $UO_2{^{2+}}$ in place of plutonium species were acquired from preliminary tests. When these conditions were applied to separate the plutonium species, two-column method was separated them successfully. However, the IC method with oxalate eluent was difficult in the separation of plutonium species due to the change of $Pu^{3+}$ and $PuO_2{^{2+}}$ to $Pu^{4+}$ and $PuO_2{^+}$ respectively.

• Analytical Science and Technology
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• v.14 no.4
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• pp.306-310
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• 2001

Ion chromatography was applied to determine iodide remained in sulfuric acid aqueous solution after adsorption procedure. Iodide was determined in 0.25 M, 0.5 M and 1 M sulfuric acid solution on time variation. Because sulfuric acid in solution plays as an oxidizer, the concentration of iodide decreased with increasing concentration or sulfuric acid. Thereafter, sulfuric acid was neutralized with sodium hydroxide. Good linearity(r=0.99999) was obtained at the range of 0-20 mg/L 1 in 0.5 M sodium sulfate matrix.

• Applied Biological Chemistry
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• v.47 no.1
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• pp.22-26
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• 2004

The ligninolytic basidiomycete, Pleurotus ostreatus K-2946, was produced a manganese peroxidase (MnP) activity when grown in liquid culture with glucose-yeast-peptone (G-Y-P) medium. However, lignin peroxidase (LiP) was not detected in this culture medium. The purification progress of MnP was purified that included chromatography on Sepharose CL-6B, Superdex 75 prep grade and Mono-Q. MnP purified by column chromatography, was 36400 dalton and a pI of 3.95. The optimal pH and temperature of the purified MnP activity were 5.0 and $55^{\circ}C$. The characteristics of MnP produced was quite similar to those of MnP 3 isoenzyme produced by other strains of P. ostreatus.

• Analytical Science and Technology
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• v.12 no.2
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• pp.89-93
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• 1999

In this study, conductivity cell and suppressor for micro-column ion chromatography were developed to analyze ions in small columns of samples. With a capillary column, the flow rate of the mobile phase is so small (usually $5{\sim}20{\mu}L/min$) that the usual conductivity cell can not be used. Therefore, we developed a new type of conductivity cell and suppressor which have small inner volumes. The conductivity cell was made with two Pt hypodermic needles (i.d. 0.010 mm) which are slightly separated (about $2{\mu}m$), and the suppressor was made of Nafion tubings. When several anions(fluoride, nitrite, nitrate, chlorate) were analyzed using developed conductivity cell and suppressor, a good chromatogram was obtained.

• Analytical Science and Technology
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• v.12 no.6
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• pp.521-527
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• 1999

In this study, capillary columns for ion chromatography were developed to analyze trace amount of ions in samples. When small I.D. capillary columns are used in ion chromatography, the typical flow rate of the mobile phase is $5{\sim}15{\mu}L/min$ and the typical column length is 50~150 mm. The capillary columns were made using RSL-300 fused silica capillary(I.D.: 0.53 mm, O.D.: 0.67 mm) and AG14 column resin(support : polystyrene-divinylbenzene, functional group : alkyl quaternary ammonium). The new conductivity cell and suppressor were also developed and made for capillary column ion chromatography. When several anions (fluoride, nitrite, nitrate, chlorate) were analyzed using these capillary columns, reproducible and good chromatograms were obtained.

• Journal of the Korean Chemical Society
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• v.32 no.2
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• pp.113-121
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• 1988

The retention mechanism and separation of various aromatic sulfonic and carboxylic acids on reversed-phase liquid chromatographic column were studied in the mobile phase containing dodecyltrimethylammonium bromide (DTAB). The retention mechanism was found to be followed the ion-interaction model where the DTAB occupies a primary layer at the stationary phase while the sample anions and other co-anions in the system compete for forming the secondary layer. The capacity factors of samples were influenced by the several factors such as pH, concentration of various organic solvents, co-anions in the mobile phase and functional groups in sample molecules. Some mixtures of organic samples were attempted to separate under optimum condition.