• Horticultural Science & Technology
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• v.32 no.3
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• pp.366-374
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• 2014

The study was conducted to investigate the possibility that epigenetic DNA methylation causes tree phenotypic variation in autotetraploids through evaluating the phenotypic variation and DNA methylation in autotetraploids occurred spontaneously from diploid trifoliate orange. Chromosome analysis confirmed that fourteen trifoliate orange trees of selected by flow cytometry were tetraploids (2n = 4X = 36) without any aneuploids. Chromomycin A3 staining determined that these trees were all autotetraploid with doubled chromosome set. Tree phenotypes, such as tree height and width, branching number, length, and angle, internode length, and leaf characteristics, varied in the autotetraploids. Chlorophyll indices were diverse in the autotetraploids, but photosynthetic rates were not significantly different. In addition, a wide range of variation was observed in stomatal density and guard cell length. Analysis of global cytosine DNA methylation showed that there was a variation of the methylation level in autotetraploids. More than half of 14 autotetraploids had at least 2 times higher methylation level than diploid trifoliate orange. The results indicate that tree phenotypic variation in autotetraploids might be related to global DNA methylation for reducing gene redundancy.

• Journal of the Korean Ceramic Society
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• v.41 no.11
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• pp.797-803
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• 2004

The heat generation tests of SiC and MO$_2$ samples by use of a microwave heating system were carried out and UO$_2$+5 wt% CeO$_2$ pellets were sintered in a microwave furnace in an oxidizing atmosphere, by taking into account the characteristics of the microwave heating obtained from the heat generation tests. The characteristics of pellets sintered in a microwave furnace were analysed and compared with those of the pellets sintered in a conventional electrical furnace. The temperature of MO$_2$ pellets with microwave heating increased quickly with input power and the variation of output power depended on the reaction characteristics of SiC and MO$_2$ with microwave. The sintered density of UO$_2$+5wt% CeO$_2$ pellets sintered in the microwave furnace was lower about 2% T.D. than that of the pellets sintered in an electrical furnace with sintering parameters. The microstructure of pellets sintered in microwave furnace has a broader pore distribution but has a larger grain size than that of the pellets sintered in the electrical furnace.

• Korean Journal of Microbiology
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• v.42 no.2
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• pp.83-88
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• 2006

This research was performed to determine the production of lovastatin and its HMG-CoA reductase activity produced by fruit bodies and mycelial liquid cultures of domestic edible mushrooms (8 fungal strains). By deter-mining TLC analysis for the confirmation of the presence of lovastatin, all the extracts from fruit bodies and mycelial liquid culture showed same Rf value (0.46), whick was identical to that of the standard lovastatin. In order to extract lovastatin from fruit body, the mixture of water/acetonitrile/methanol was chosen as the most effective solvent. Extracts from fruit body and mycelial liquid culture of pleurotus ostreatus produced the high-est lovastatin 0.98 mg/g based on dry biomass, and 21.90 mg/L, respectively. In the inhibition rate of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, the highest was obtained in P. ostreatus as 67.8% among fruit bodies, and the rates of mycelial liquid culture extracts from P. ostreatus and Laetiporus sulphureus were 37.2% and 29.1%, respectively. Unusually L. sulphureus showed high inhibition rate with low content of lovastatin due to the contribution of campesterol and gamma-sitosterol with hypocholesterolemic activity as metabolites.

• Journal of Dental Rehabilitation and Applied Science
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• v.31 no.1
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• pp.60-66
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• 2015

Screw loosening and screw fracture of abutment is one of most frequent mechanical complications in implant restoration. Fractured fragments in implant restoration like abutment and screw should be completely removed and the procedure needs minimal damage to the fixture of implant. In some cases, it could fail to remove fractured fragments and cause a lot of damage to the fixture of implant. These situations could render implant unusable at the worst. This article describes three different situations and simple techniques for successful removal of fractured fragments without damage of implants. The procedures used are described in this clinical report.

• Journal of the Korean Society of Hazard Mitigation
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• v.4 no.2 s.13
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• pp.47-52
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• 2004

Whereas The masonry buildings are safe under gravity loads, most of the masonry buildings in Korea have many structural defects under a lateral load due to an earthquake acceleration. But there is no earthquake resistant design code for the Masonry in Korea. Therefore it may be necessary to be set up an seismic code and be suggested for reinforcing methods for existing masonry buildings. The purpose of this paper is to investigate seismic capacity of reinforced masonry buildings subjected to earthquake load. The typical two models of the masonry building in Korea are selected through a site investigation. On the basis of test results, the fiber reinforcing effect of the two models was considerable. The maximum base shear force and deformation capacity for RM were remarkably increased. It was found that the pier rocking failure was a dominant mode for the RM buildings during a seismic excitation.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.429-432
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• 2000

Chitosan scaffold is widely applied to drug delivery and tissue engineering. We have developed chitosan scaffolds, with various pore size, by differing freezing temperature and duration of ultraviolet (UV) irradiation, for reconstructing skin equivalent. Chitosan scaffold was coated with type I collagen, fibronectin and basic fibroblast growth factor (bFGF) in various combinations and concentrations, to evaluate the effect of extracellular matrix (ECM) and bFGF on cell adhesion, growth and differentiation of dermal fibroblasts. Human dermal fibroblasts, isolated from newborn foreskin and passaged between 3 and 5, were seeded on the top of scaffolds and cultivated for 2 weeks. We examined the morphology and the secretion of ECM of fibroblasts using scanning electron microsopy (SEM) and histochemistry. A stellate morphology of fibroblasts were seen in all groups. The scaffold coated with either type I collagen and bFGF or type I collagen and fibronectin, however, showed the best condtion of dermal fibroblasts, in that the highest cell number and ECM secretion were seen. On the contrary, scaffolds coated with all three factors, type I collagen, bFGF and fibronectin, showed lower number of cells and ECM secretion than scaffolds with two factors. There was a tendency of dose-dependence in all three factors for fibroblast growth and ECM secretion. In conclusion, we may suggest that chitosan scaffold coated with either type I collagen/bFGF or type I collagen/fibronectin could provide more favorable environment for the growth and differentiation of dermal fibroblasts.

• The Journal of the Acoustical Society of Korea
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• v.33 no.3
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• pp.153-162
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• 2014

Empirical prediction method of the acoustic load on the fairing is based on jet experimental data on the basis of similarity principle. Representative empirical prediction method, DSM-II(Distributed Source Method-II), is a distributing source method along the jet plume. But the empirical prediction model is limited to reflect the impingement source in real environment because it is based on the free jet data. So, we propose a empirical prediction method considering the impinging jet effect by adding a impingement source in the existing prediction method. Considering the additional source's displacement, spectrum, strength and directivity, we calculate the acoustic load on the KSR-III(Korean Sounding Rocket-III) rocket and compare the results with the existing method and experiment data.

• Journal of the Korean Chemical Society
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• v.27 no.4
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• pp.287-293
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• 1983

Free radical-initiated copolymerizations of diethyl ${\alpha}$-phenylvinyl phospbate (DEPVP) with acrylonitrile (AN) and maleic anhydride (MAnh) were studied. The monomer reactivity ratios for AN/DEPVP pair, determined at $70^{\circ}C$ in bulk using benzoyl peroxide as an initiator, were;$ r_1(AN) = 0.77, r_2(DEPVP) = 0.002$. The values of the Alfrey-Price constants, Q and e, for DEPVP were calculated to be 0.012 and -1.35, respectively. Free radical-initiated copolymerization of MAnh/DEPVP pair in chloroform at $70^{\circ}C$ produced 1 : 1 alternating copolymers regardless monomer feed composition with the highest copolymerization rate at the molar ratio of MAnh : DEPVP = 7 : 3. The equilibrium constant of a charge-transfer complex between DEPVP and MAnh in deutrated chloroform, determinated at room temperature by transformed Benesi-Hildebrand NMR method, was 0.085 l/mol. The reduced viscosity of copolymers of AN/DEPVP pair decreased as the content of DEPVP units increased, while that of MAnh/DEPVP pair remained more or less constant.

• Journal of the Korea institute for structural maintenance and inspection
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• v.17 no.5
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• pp.40-48
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• 2013

Eco deck plate system is a construction method that deconstruction of galvanized steel sheets is possible by integrating steel-wire-integrated girders and the galvanized steel sheets with bolts. Therefore, compared with previous steel-wire-integrated deck plates which were joined by welding, the system is acknowledged as the construction method possible management and repair. This study conducted an experimental research by manufacturing total 24 full size specimens in a same condition for 12-shape specimens by two parts to evaluate structural behaviors of the eco deck plates. In the results after the test, permissible deflection for the construction load action was shown to be values under design values and satisfactory. The processing of lattice steel wires was presented to be structurally advantageous in being manufactured by cutting downward. Also, in case of a specimen that D13 as a steel wire was used, destruction occurred at the welding part of the bottom steel wire and the lattice steel wire, so improvement measures for the welding in factory manufacture are necessary.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.6
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• pp.4108-4114
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• 2015

An image acquisition by using an optical mirror is called as a catadioptric method. The catadioptric imaging method is generally used for acquisition of 360-degree all directional visual information in an image. An exemplar omnidirectional optical mirror is the bowl-shaped hyperbolic mirror. In this paper, a single camera omnidirectional stereo imaging method is studied with an additional concave lens. It is possible to obtain 3 dimensional coordinates of environmental objects from the omnidirectional stereo image by matching the stereo image having different view points. The omnidirectional stereo imaging system in this paper is cost-effective and relatively easy for correspondence matching because of consistent camera intrinsic parameters in the stereo image. The parameters of the imaging system are extracted through 3-step calibration and the performance for 3-dimensional coordinates reconstruction is verified through experiments in this paper. Measurable range of the proposed imaging system is also presented by depth-resolution analysis.