• Journal of Life Science
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• v.32 no.11
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• pp.833-840
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• 2022

Chronic inflammation has been shown to be closely associated with tumor development and progression. Nuclear factor kappa B (NF-κB) is composed of a family of five transcription factors. NF-κB signaling plays a crucial role in the inflammatory response and is often found to be dysregulated in various types of cancer, making it an attractive target in cancer therapeutics. In this study, CDC-like kinase 3 (CLK3) was identified as a novel kinase that regulates the NF-κB signaling pathway. Our data demonstrate that CLK3 inhibits the canonical and non-canonical NF-κB pathways. Luciferase assays following the transient or stable expression of CLK3 indicated that this kinase inhibited NF-κB activation mediated by Tumor necrosis factor-alpha (TNFα) and Phorbol 12-myristate 13-acetate (PMA), which are known to activate NF-κB signaling via the canonical pathway. Consistent with data on the ectopic expression of CLK3, CLK3 knockdown using shRNA constructs increased NF-κB activity 1.5-fold upon stimulation with TNFα in HEK293 cells compared with the control cells. Additionally, overexpression of CLK3 suppressed the activation of this signaling pathway induced by NF-κB-inducing kinase (NIK) or CD40, which are well-established activators of the non-canonical pathway. To further examine the negative impact of CLK3 on NF-κB signaling, we performed Western blotting following the TNFα treatment to directly identify the molecular components of the NF-κB pathway that are affected by this kinase. Our results revealed that CLK3 mitigated the phosphorylation/activation of transforming growth factor-α-activated kinase 1 (TAK1), inhibitor of NF-κB kinase alpha/beta (IKKα/α), NF-κB p65 (RelA), NF-κB inhibitor alpha (IκBα), and Extracellular signal-regulated kinase 1/2-Mitogen-activated protein kinase (ERK1/2-MAPK), suggesting that CLK3 inhibits both the NF-κB and MAPK signaling activated by TNFα exposure. Further studies are required to elucidate the mechanism by which CLK3 inhibits the canonical and non-canonical NF-κB pathways. Collectively, these findings reveal CLK3 as a novel negative regulator of NF-κB signaling.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2010.07a
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• pp.36-39
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• 2010

멀티 포맷 방송용 모니터는 SDI 신호뿐만 아니라 HDMI, DVI, Component, Composite로 전송되는 영상, 음성, 부가 데이터를 보여주는 모니터로 방송용 레퍼런스 모니터로 사용되고 있다. 특히 부가 데이터 중에서 Closed Caption의 경우 북미에서는 EIA-608과 EIA-708 두 가지 표준이 있고, 세부적으로 네 가지의 방법으로 전송되는데 일반적인 방송용 모니터에는 적용되어 있는 것이 극히 드물다. 또한 SDI 신호로 전송되는 Closed Caption 데이터를 Decoding하는 상용 IC는 거의 없는 수준이다. 이에 본 논문에서는 SDI로 전송되는 다양한 방식의 Closed Caption 데이터를 모두 표시하기 위한 방법을 제안하였다. 먼저 VBI (Vertical Blanking Interval) 에 아날로그 Waveform 형태로 입력되는 경우 데이터의 신뢰도를 높이기 위해 Clock Run In을 실시간으로 검출 할 수 있는 구조를 제안하고 FPGA (Field Programmable Gata Array)로 구현하였다. 또한 VANC (Vertical Ancillary Space)로 들어오는 Caption데이터의 경우 특히 EIA-708 처럼 많은 데이터가 입력되는 경우 실시간으로 처리하기 위해서 기존의 I2C와 같은 느린 전송 방법이 아닌 FPGA와 프로세서 간에 메모리를 직접 Access 할 수 있는 방법을 제안하였다. 본 논문에서 제안 한 방법을 FPGA로 구현하였고, 실제 미국이나 캐나다 방송국에서 사용하는 Caption 인코더 장비 뿐만아니라 방송 콘텐츠를 직접 이용하여 동작 상태를 검증하였다.

• The Journal of the Acoustical Society of Korea
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• v.22 no.3
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• pp.169-174
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• 2003

A novel subspace speech enhancement using subband whitening filter is proposed. Previous subspace speech enhancement method either assumes additive white noise or uses whitening filter as a pre-processing for colored noise. The proposed method tries to minimize the signal distortion while reducing residual noise by processing the signal using subband whitening filter. By incorporating the notion of subband whitening filter, spectral resolution in Karhunen-Loeve(KL) domain is improved with the negligible additional computational load. The proposed method outperforms both the subspace method suggested by Ephraim and the spectral subtraction suggested by Boll in terms of segmental signal-to-noise ratio (SNRseg) and perceptual evaluation of speech quality (PESQ).

• Journal of the Korea Society of Computer and Information
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• v.3 no.2
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• pp.77-84
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• 1998

The continuous time signal system by development of CMOS technology have been receiving consideration attention. In this paper, Low pass filter using CMOS floating resistor have been designed with cut off frequency for speech signal processing. Especially a new floating resistor consisting entirely of CMOS devices in saturation has been developed. Linearity within $\pm$0.04% is achieved through nonlineartiy via current mirrors over an applied range of $\pm$1V. The frequency response exceeds 10MHz, and the resistors are expected to be useful in implementing integrated circuit active RC filters. The low pass filter designed using this method has simpler structure than switched capacitor filter. So reduce the chip area. The characteristics of the designed low pass filter using this method are simulated by pspice program.

• The Journal of the Acoustical Society of Korea
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• v.21 no.7
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• pp.606-613
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• 2002

In this paper we applied a transmit beamforming technique to the underwater acoustic communication system for high rate data transmission. A prototype transmit system was designed and implemented with the general purpose DSP processor and multiple digital-to-analog converters. The performances of the implemented system were evaluated by the experiment in water tank. In order to simplify the procedure the channel coding and equalizer were omitted. And the simplest OOK (On-Off Keying) technique in digital communication methods was applied. The experimental result shows that the transmission data rate is higher about 3 times in the case of 5 hydrophone transmitting may than 1 hydrophone transmitter at bit error rate 10/sup -2/. We verified that the maximum data rate was 400 bps for speech signal transmission in water tank.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.10a
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• pp.113-116
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• 2015

This paper presented about the system for controlling a wireless power transmission using bluetooth protocol. Bluetooth protocol has been applied in many fields that communicate with data and audio signal in short range. Recently, however, Bluetooth low energy(BLE) more simple than the existing protocol is standardized and is widely used in medical applications and consumer electronics that handle small amount of sensor data and transmit by the low power control signal. It has also been adopted as the standard for the control in the wireless power transfer system. In this paper, We analysed and described the bluetooth low energy protocol techniques for controlling the wireless power transfer system.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.11
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• pp.2474-2479
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• 2011

As society has developed rapidly toward a highly advanced digital information age, a multimedia communication service for acquisition, transmission and storage of image data as well as voice has being commercialized. However, image data is always corrupted by various noises during image processing, so researches for removing noises have been continued until now. In this paper, in order to remove impulse noise we proposed modified adaptive switching median filter that consists of two stages: noise detection and noise removal. Proposed algorithm only processes noise pixels and these noise pixels are replaced by filter output, so proposed algorithm performs well not only removes noise but also preserves edge information. Also we compare existing methods using PSNR(peak signal to noise ratio) as the standard of judgement of improvement effect and choose conventional algorithms to compare with our proposed method.

• The Journal of the Acoustical Society of Korea
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• v.23 no.1
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• pp.47-53
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• 2004

We propose a new speech enhancement algorithm based on left-right Hidden Markov Model (HMM) with state decision using Log-likelihood Ratio Test (LRT). Since the conventional HMM-based speech enhancement methods try to improve speech quality for all states, they introduce huge computational loads inappropriate to real-time implementation. In the left-right HMM, only the current and the next state are considered for a possible state transition so to reduce the computational complexity. In this paper, we propose a method to decide the current state by using the LRT on the previous state. Experimental results show that the proposed method improves the speed up to 60% with 0.2∼0.4 dB degradation of speech quality compared to the conventional method.

• Journal of Korea Society of Industrial Information Systems
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• v.6 no.2
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• pp.47-53
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• 2001

Real-time multimedia transmit services like internet broadcasting, web-based education, chatting, conference, circumference observation etc. by using internet are given a great deal of weight on most recently internet services. In these way, various internet services are presented and there applications are also gradually spreaded because of improvement MPEG and streaming processing techniques. But currently internet service is a development of system which has independent function of above services. And a research and development of system which have a multipurpose internet service are insufficiency. In this paper, we design and develop system(RealCam SUN A/V) for transmitting images and voices based on MPEG4 streaming. As a result of our research, it made possible to transmit images with 15 frame/sec in 160×120 frame size at 56Kbps and 30 frame/sec in 640×480 frame size at 512Kbps. And also we can apply this system as multipurpose function to circumference observation and internet broadcasting etc., by transmitting voices, images and control signal simultaneously.

• The Journal of the Acoustical Society of Korea
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• v.14 no.4
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• pp.46-63
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• 1995

This thesis described an unlimited vocabulary connected speech recognition system using Time Delay Neural Network(TDNN). The recognition unit is the diphone unit which includes the transition section of two phonemes, and the number of diphone unit is 329. The recognition processing of korean connected speech is composed by three part; the feature extraction section of the input speech signal, the diphone recognition processing and post-processing. In the feature extraction section, the extraction of diphone interval in input speech signal is carried and then the feature vectors of 16th filter-bank coefficients are calculated for each frame in the diphone interval. The diphone recognition processing is comprised by the three stage hierachical structure and is carried using 30 Time Delay Neural Networks. particularly, the structure of TDNN is changed so as to increase the recognition rate. The post-processing section, mis-recognized diphone strings are corrected using the probability of phoneme transition and the probability o phoneme confusion and then the eojeols (Korean word or phrase) are formed by combining the recognized diphones.