• Journal of the Korea Convergence Society
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• v.10 no.6
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• pp.65-71
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• 2019

The propose of this study has been conducted to examine expression of c-Myc and Thymosin-${\beta}4$ in liver cirrhosis model from liver fibrosis and For the method of study, the experiment was conducted in 2 groups; liver cirrhosis model experiment group due to liver fibrosis and control group with distilled water. This study outcome showed that liver cirrhosis model experiment group had significantly higher expression of c-Myc and Thymosin-${\beta}4$. with changes to hepatic tissue of special staining and electron microscopy. In conclusion, in clinical tests regarding liver function, molecular evaluation of c-Myc and Thymosin-${\beta}4$ and their expression along with serological change and histological assessment can be utilized as a reference for diagnosing liver disease for prevention and diagnosis of the disease, Based on this research in the future, we will carry out an in-depth study by adding the types of experimental groups and related genes.

• Journal of Life Science
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• v.14 no.2
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• pp.325-330
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• 2004

The RAD3 gene of Saccharomyces cerevisiae is required for excision repair and is essential for cell viability. RAD3 encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. To examine the extent of conservation of structure and function of RAD3 during eukaryotic evolution, the RAD3 homolog gene was isolated by screening of genomic DNA library. The isolated gene was designated as HRD3 (Homologue of RAD3 gene). The over-expressed HRD3 protein was estimated to be a 75 kDa in size which is in good agreement with the estimated by the nucleotide sequence of the cloned gene. Two-dimensional gel electrophoresis showed that a number of other protein spots dramatically disappeared when the HRD3 protein was overexpressed. The overexpressed RAD3 protein showed a toxicity in E. coli host, suggesting that this protein may be involved in the inhibition of protein synthesis and/or degradation of host protein. To determine which part of HRD3 gene contributes to the toxicity in E. coli, various fusion plasmids containing a partial sequence of HRD3 and lac'Z gene were constructed. These results suggest that the C-terminal domain of HRD3 protein may be important for both toxic effect in E. coli and for its role in DNA repair in S. pombe.

• Journal of Aquaculture
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• v.10 no.1
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• pp.33-37
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• 1997

construct containing reporter gene(pFV4CAT) regulated by carp $\beta$-actin promoter was microinjected into the one-cell stage egg of mud loach (Misgurnus mizolepis), and was successfully expressed, possibly by the integration into the genome. Both mean hatching success and early survival of the microinjected groups were not significantly different with those of control groups (P>0.05). The incidence of transgene was ranged from 7 to 48% based on the PCR and/or Southern blot analyses with the DNA prepared from fin or blood tissue. The spatial and temporal patterns of expression of the pFV4CAT gene, measured by in situ immunohistochemical analysis peroxidase-conjugated anti-CAT antibody, were variable among the experimental individuals. These results suggest that carp $\beta$-actin promoter is effective to express other transgene in mud loach, such that this promoter can be useful in the generation of valuable transgenic mud loach.

• Journal of the Korea Convergence Society
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• v.8 no.1
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• pp.19-23
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• 2017

Energy is a vital resource for the economic growth and the social development for any country. As the industry becomes more sophisticated and the economy more grows, the electricity demand is increasing. So forecasting electricity demand is an important for electricity suppliers. Forecasting electricity demand makes it possible to distribute electricity demand. As the market for Negawatt market began to grow in Korea from 2014, the prediction of electricity consumption demand becomes more important. Moreover, power consumption forecasting provides a way for demand management to be directly or indirectly participated by consumers in the electricity market. We use Genetic Algorithms to predict the energy demand of the fishing industry in Jeju Island by using GDP, per capita gross national income, value add, and domestic electricity consumption from 1999 to 2011. Genetic Algorithm is useful for finding optimal solutions in various fields. In this paper, genetic algorithm finds optimal parameters. The objective is to find the optimal value of the coefficients used to predict the electricity demand and to minimize the error rate between the predicted value and the actual power consumption values.

• Korean Journal of Animal Reproduction
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• v.18 no.3
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• pp.175-182
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• 1994

Rat $\beta$-casein 유전자와 사람 성장호르몬 (hGH) 유전자의 융합유전자를 생쥐 수정란의 웅성전핵에 미세주입하여 형질전환생쥐 (transgenic mouse) 6계통을 확립하였다. 이들로부터 사람성장호르몬(hGH) 유전자의 발현 여부를 조사한 결과, 6계통중 4계통의 생쥐 유즙에서 hGH가 2~900 ng/ml 수준으로 발현되고 있었으며, 혈중에서는 hGH가 검출되지 않았다. 따라서 이들 형질전환생쥐에서 사람성장호르몬이 우선특이적으로 발현, 분비되고 있음을 알 수 있었다. 한편, 사람성장호르몬의 발현이 확인된 두 계통 (ChGH2-2, CChGH2)의 형질전환생쥐를 대상으로 하여 세대별, 산차별로 유즙내 사람성장호르몬의 함량을 조사한 결과, 3세대에 걸쳐, 또한 제1세대에서의 세차례 반복된 비유기에도 유즙내 사람성장호르몬은 지속적으로 분비되고 있었다. 이상의 결과는 형질전환생쥐에서의 외래유전자의 발현성은 반복되는 비유기와 여러 세대에 걸쳐 안정적으로 유지됨을 보여 주고 있다.

• Journal of the Institute of Convergence Signal Processing
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• v.3 no.3
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• pp.63-69
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• 2002

Detailed routing is a problem assigning each net to a track after global routing. The most popular algorithms for detailed routing include left-edge algorithm, dogleg algorithm, and greedy channel routing algorithm. In this paper we propose a genetic algorithm searching solution space for the detailed routing problem. We compare the performance of proposed genetic algorithm(GA) for detailed routing with that of greedy channel routing algorithm by analyzing the results of each implementation.

• Proceedings of the Korea Institute of Convergence Signal Processing
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• 2001.06a
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• pp.269-272
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• 2001

In this paper, we proposed the face recognition algorithm which can use a security system. The distance and angle of the face features are used in the conventional method, but the proposed method used the genetic algorithm which selects image to best fit the input image in the database images. The performance of proposed algorithm is verified through the simulation. The proposed one has good performance.

• Journal of the Institute of Convergence Signal Processing
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• v.3 no.2
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• pp.15-22
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• 2002

In this paper, we proposed the face recognition algorithm which can be used for a security system. The distance and angle of the face features are used in the conventional method, but the proposed method used the genetic algorithm which selects image to best fit the input image in the database images. The performance of proposed algorithm is verified through the simulation. The results of proposed method show good performance.

• Journal of the Institute of Convergence Signal Processing
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• v.3 no.2
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• pp.89-95
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• 2002

Global routing is to assign each net to routing regions to accomplish the required interconnections. The most popular algorithms for global routing inlcude maze routing algorithm, line-probe algorithm, shortest path based algorithm, and Steiner tree based algorithm. In this paper we propose weighted network heuristic(WNH) as a minimal Steiner tree search method in a routing graph and a genetic algorithm based on WNH for the global routing. We compare the genetic algorithm(GA) with simulated annealing(SA) by analyzing the results of each implementation.

• The Journal of the Convergence on Culture Technology
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• v.8 no.4
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• pp.415-422
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• 2022

The heterogeneous Onju citrus genes (A=20.57, C=32.71, G=30.01, U=16.71%) and coffee genes (A=20.66, C=31.76, G=30.187, U=16.71%) have the same genetic ratio of 95% or more. It is known that gene compatibility is generally not possible with this group. However, it can be grafted if the conditions of Chargaff rule and Shannon Entropy are met with gene functional-similarity of more than 95%, and it becomes a new breed of Coffrange. We calculated the world's first BCJM matrix for DNA-RNA and published it in US patents and international journals. All animals and viruses are similar to human genes. Based on this, it was announced in June in the British matrix textbook by solving the genetic characteristics of COVID-19 and the human body. In plants, it is treated with BCJM-Transposon treatment, a technique that easily changes gene location. Simulation predicted that the matrix could be successful with Cut & Paste and Transpose.