Browse > Article
http://dx.doi.org/10.15207/JKCS.2019.10.6.065

A Study on the Expression of Thymosin-β4 and c-Myc mRNA in the Model of liver cirrhosis with fibrosis  

Kim, Jean-Soo (Department of Clinical Laboratory Science, Daejeon Health Institute of Technology)
Park, Un-Kyu (Department of Clinical Laboratory Science, Daejeon university)
Publication Information
Journal of the Korea Convergence Society / v.10, no.6, 2019 , pp. 65-71 More about this Journal
Abstract
The propose of this study has been conducted to examine expression of c-Myc and Thymosin-${\beta}4$ in liver cirrhosis model from liver fibrosis and For the method of study, the experiment was conducted in 2 groups; liver cirrhosis model experiment group due to liver fibrosis and control group with distilled water. This study outcome showed that liver cirrhosis model experiment group had significantly higher expression of c-Myc and Thymosin-${\beta}4$. with changes to hepatic tissue of special staining and electron microscopy. In conclusion, in clinical tests regarding liver function, molecular evaluation of c-Myc and Thymosin-${\beta}4$ and their expression along with serological change and histological assessment can be utilized as a reference for diagnosing liver disease for prevention and diagnosis of the disease, Based on this research in the future, we will carry out an in-depth study by adding the types of experimental groups and related genes.
Keywords
Hematoxylin; Eosin; Masson's Trichrome; Reticulum; c-Myc; Thymosin-${\beta}4$; Convergence;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Y. S. Choi. (2017). The Regulatory Role of Dickkopf-1 as a Target of Oncogenic Transforming Growth Factor-${\beta}1$-Smad3 Pathway in Human Lung Cancer Cells. Master's dissertation, Gangwon univrsity Graduate school, Chuncheon
2 J. H. Park. (2016). The association of single nucleotide polymorphisms in the TGF-${\beta}1$ and RAGE genes with polycystic ovary syndrome. Master's dissertation, Cha university General Graduate Shool, 2-3. Pocheon.
3 H. Rouach, M. Clement, M. T. Ofanelli, B. Janvier, J. Nordmann & R. Nordmann. (1983). Hepatic lipid peroxidation and mitochondrial susceptibility to peroxidative attacks during ethanol inhalation and withdrawal. Biochem. Biophys, Acta, 753, 439-444.   DOI
4 T. L. Low & A. L. Goldstein. (1982). Chemical characterization of thymosin beta-4. J. Biol. Chem, 257, 1000-1006.   DOI
5 M. J. Kim. (2017). A study on Expression of Thymosin ${\beta}4$ in Cervical Cancer induced Human Papilloma Virus. Master's dissertation, Konyang university Graduate school. Daejeon.
6 K. C. Hong. (2015). Overexpression and characterization of thymosin beta-4 in Escherichia coli. Master's dissertation, Dongwee univrsity Graduate school, Busan.
7 K. O. Hong. (2016). Thymosin b4 induces proliferation, invasion, and epithelial-to-mesenchymal transition of oral squamous cell carcinoma. Doctoral dissertation, Seoul univrsity Graduate school, Seoul.
8 S. H. Yoon. (2008.). Expression of Thymosin-${\beta}4$ in odontoblast differentiation of the mouse. Doctoral dissertation. Josun Univ. Graduate school. Gwangju.
9 D. Safer, M. Elzinga & V. T. Nachmias. (1991). Thymosin beta4 and Fx, an actin-sequestering peptide, are indistinguishable. J. Biol. Chem, 266, 4029-4032.   DOI
10 D. Safer, R. Golla & V. T. Nachmias. (1990). Isolation of a 5-kilodalton actin-sequestering peptide from human blood platelets. Proc. Natl. Acad. Sci. USA, 87, 2536-2540.   DOI
11 D. S. Grant, J. L. Kinsella, M. C. Kibbey, S. LaFlamme, P. D. Burbelo, A. L. Goldstein & H. K. and Kleinman. (1995). Matrigelinducesthymosin beta4 gene in differentiating endothelial cells. J Cell Sci, 108, 3685-3694.   DOI
12 D. S. Grant, W. Rose, C. Yaen, A. Goldstein, J. Martinez & H. Kleinman. (1999). Thymosin-${\beta}4$ enhances endothelial cell differentiation and angio genesis. Angio genesis, 3, 125-135.
13 J. Gomez-Marquez, J. I. Pedrares, A. Otero & R. Anadon. (1993). Prominent expression of the actin-sequestering peptide Fx gene in the hippo campal region of ratbrain. Neuroscience Letters, 152, 41-44.   DOI
14 J. Gomez-Marquez, F. F. delAmo, P. Carpintero & R. Anadon. (1996). High levels of mouse thymosin b4 mRNA in differentiating P19 embryonic cells and during development of cardiovascular tissues. Biochim BiophysActa, 1306, 187-193.
15 R. Anadon, I. R. Moldes, P. Carpintero, G. Evangelatos, E. Livianou, L. Leondiadis, I. Quintela, M. C. Cervino, J & Gomez-Marquez. (2001). Differential expression of thymosin b4 and b10 during rat cerebellum postnatal development. Brain Res, 894, 255-265.   DOI
16 E. A. Clark, T. R. Golub, E. S. Lander & R. O. Hynes. (2000). Genomics analysis of metastasis reveals an essectial role for RhoC. Nature, 406, 532-535.   DOI
17 D. Philp, A. L. Goldstein & H. K. Kleinman. (2004). Thymosin ${\beta}4$ promotes angiogenesis, wound healing, and hair follicle development. Mech Ageing Dev, 125, 113-115.   DOI
18 T. Huff, C. S. Muller, A. M. Otto, R. Netzke & E. Hannappel. (2001). beta-Thymosins, small acidic peptides with multiple functions. Int J Biochem Cell Biol, 33, 205-209.   DOI
19 W. S. Wang, P. M. Chen, H. L. Hsiao, S. Y. Ju & Y. Su. (2003). Over expression of the thymosin beta-4 gene is associated with malignant progression of SW480 colon cancer cells. Oncogene, 22, 3297-3306.   DOI
20 K. M. Malinda, A. L. Goldstein & H. K. Kleinmman. (1997). Thymosin beta 4 stimulates directional migration of human umbilical vein endothelial cells. FASEG J, 11, 474-481.   DOI
21 T. Kobayashi, F. Okada, N. Fujii, N. Tomita, S. Ito & H. Tazawa. (2002). Thymosin-beta 4 regulates motility and metastasis of malignant mouse fibrosarcoma cells. Am J Pathol, 160, 869-882.   DOI
22 K. H. Kim, K. J. Jo & Y. Go. (2011). Understanding and technique of genetics, Korea medical book, 203-205.
23 S. J. Kim. (1993). c-myc DNA Amplification and DNA Ploidy Analysis in Cervical Cancer of Uterus, Obstetrics &Gynecology Science, 75-88.   DOI
24 M. S. OK. (2012). Analysis of Expression Patterns of Thymosin ${\beta}4$ and CD133 in Normal Stomach, Korea society of life science, 1415-1419.
25 Y. B. Yu. (2012). Protective Effects of Cordyceps militaris on Carbon tetrachloride-Induced Liver Damages. Doctoral dissertation, Inje university. Gimhea.
26 J. H. Lee, C. G, Yoon & S. I. Lee. (1993). Effects of dietary protein on the changes of lipoprotein fractions in carbon tetrachloride-treated rats. J. Korean Soc. Food Sci. Nutr., 22, 127-131.
27 J. L. Kenneth & D. S. Thomas. (2001). Analysis of relative gene expression data using real-time Quantitative PCR and the 2-DDCT method. Methods, 25, 402-408.   DOI
28 S. H. Hong, (2000). Effects of Vitamin E on Carbon Tetrachloride : Induced Acute Hepatotoxicity in the Rat, Master's dissertation, Konkuk universty graduate school. Seoul.
29 C. J. McClain, Z. Song, S. S. Barve, D. B. Hill & I. Deaciuc, (2004). Recent advances in alcoholic liver disease IV. dysregulated cytokine metabolism in alcoholic liver disease. Am. J. Physiol. Gastrointest. Liver Physiol. 287(1), 497-502.   DOI
30 M. D. Wheeler, M. Nakagami, B. U. Bradford, T. Uesugi, R. P. Mason, H. D. Connor, A. Dikalova, M. Kadiiska & R. G. Thurman. (2001). Overexpression of manganese superoxide dismutase prevents alcohol-induced liver injury in the rat. J. Biol. Chem, 276(1), 36664-36672.   DOI
31 M. J. Kim. (2017). A study on Expression of Thymosin ${\beta}4$ in Cervical Cancer induced Human Papilloma Virus. Master's dissertation, Konyang university graduate school. Daejeon.
32 J. S. Kim & S. K. Choi. (2018). A study of Expression of TGF-${\beta}1$, c-Myc, Erb-B2 and Thymosin-${\beta}4$ Gene in Alcoholic Liver Damage Tissue. Journal of The Korea Convergence Society. 9(5), 91-97   DOI
33 H. J. Han. (2019). The study on the role of thymosin beta 4 in PrP (106-126) mediated neurotoxicity. Master's dissertation, Jbnu university Graduate school Jeonju.
34 S. H. Min. (2017). Clinical and Genetic Characteristics of MYC Gene Aberrations in Patients with Multiple Myeloma. Master's dissertation, Ulsan university Graduate school Ulsan.
35 Y. J. Jo. (2017). Transcriptional Control of c-Myc on VKORC1 Gene Expression, Master's dissertation, chungbuk university Graduate school Cheongju.