• Journal of Life Science
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• v.28 no.2
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• pp.162-169
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• 2018

Many cytoplasmic proteins are targeted to the cytoplasmic membrane of the trans-Golgi network (TGN) via an N-terminal short helix. We previously showed that the 20 N-terminal amino acids of Aplysia phosphodiesterase 4 (ApPDE4) long form are sufficient for its targeting to the plasma membrane and the TGN. The N-terminus of the ApPDE4 long form binds to PI4P and sulfatide in vitro. Therefore, in order to decipher the roles of sulfatide in Golgi complex targeting, we examined the cellular localization of sulfatide-binding peptides. In this study, we found that enhanced green fluorescent protein (EGFP) fused to the C-terminus of modified sulfatide- and heparin-binding peptides (mHSBP-EGFP) was localized to the TGN. On the other hand, its mutant, in which tryptophan was replaced with an alanine, leading to the impairment of heparin and sulfatide binding, was localized to cytosol. We also found that the TGN targeting of mHSBP-EGFP is impaired by the treatment of antimycin A, phenylarsine oxide (PAO), and adenosine but not a high concentration of wortmannin. These results suggest that PAO and adenosine-sensitive kinases, including phosphatidylinositol 4-kinase II, may play key roles in the recruitment of mHSBP-EGFP.

• The Korean Journal of Community Living Science
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• v.28 no.3
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• pp.377-390
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• 2017

This study identified technological trends through an analysis of patents for the industrialization of personal protection and convenience equipment using information and communication technology (ICT) as a part of efforts to prevent farm work-related disasters. The analysis was conducted on patents registered and published between January 1974 and May 2016 by the world's five largest intellectual property offices, including the KIPO, USPTO, JPO, EPO, and SIPO. The results of the analysis indicate that the US (36.8%) and South Korea (30.9%) led technological research and development (R&D) with frequent patent applications. An analysis of the technological market revealed that these countries are in the growth and maturity stages, in which the number of patents and number of patent applicants grow rapidly. In terms of the technological market shares of major countries, the US recorded the highest market shares in the field of sensing systems for workers' dangerous conditions and convenience protection equipment based on the internet of things (IoT) convergence. South Korea marked the highest share of 41.8% in the field of sensing devices for dangerous conditions in the working environment. An analysis of the trend of patent applications by specific technologies disclosed the following results: sensing systems for workers' dangerous conditions accounted for the highest share (49.2%), followed by IoT convergence-based convenience protection equipment (26.3%) and sensing devices for dangerous conditions in the working environment (24.6%). Based on this study, ICT-based personal protection and convenience equipment technologies are expected to be actively developed in the future. It will be necessary to secure national competitiveness through R&D investments and commercialization in personal protection and convenience equipment appropriate for farm work as well as through the acquisition of patent technologies and intellectual property rights.

• Journal of Life Science
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• v.8 no.3
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• pp.263-271
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• 1998

One of the better-characterized transcription factor of E. coli is the cAMP receptor protein(CRP) and the CRP binds cAMP and DNA. The cAMP-CRP complex is involved in regulation of many genes at bacteria. The cAMP-CRP regulatory element represents, in some respects, a global regulatory network. The aim of this work was to study the structure and the mechanisms controlling the expression of CRP in Serratia marcescens. We have been get 5 different clones from Serratia which stimulated the cells to use maltose as a sole carbon source in E. coli TP2139. The crp gene clone, pCKB12, was confirmed by Southern hybridization with E. coli crp gene. The location of the crp gene was determined by construction subclones carrying various portions of pCKB12. To investigate the potential role of CRP in E. coli, lacZ fused plasmids were constructed and investigated the ${\beta}$-galactosidase activity of the fused plasmid. The Serratiamarcescens cAMP receptor protein can substitute the E. coli CRP in transcriptional activation at the lacZ gene. These results suggest that Serratia marcescens cAMP receptor protein complex functions to regulate several promoters in E. coli.

• Journal of the Korean earth science society
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• v.39 no.1
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• pp.89-102
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• 2018

We analyzed the meteorological and radiation characteristics of Seoul metropolitan area using data from energy flux towers that were installed and operated by the Weather Information Service Engine (WISE). The meteorological and radiation variables included temperature, pressure, wind speed, wind direction, relative humidity, surface temperature, rainfall amount, upward and downward solar radiation, upward and downward longwave radiation, albedo and emissivity from 14 energy flux stations located in the Seoul metropolitan area from July 2016 to July 2017. According to the monthly data during the period, the albedo is low and emissivity is high at the Jungnang station in the urban and opposite at Bucheon station in the suburban area. For a station in natural state, the albedo was higher than urban stations because solar radiation reflects effectively. Relatively high temperatures were shown at stations located in urban area with low albedo and high emissivity, in general. However, temperature was high at Gajwa and Ttukseom stations, the albedo was relatively high due to the station environment surrounded by glass wall buildings and the Han river. In the station located in suburban area, both emissivity and temperature were low. Among these stations, Bucheon station had the highest emissivity values because the surface temperature was relatively lower than that of the suburban area. As a result, the albedo decreased and the emissivity increased at stations in urban areas. Additionally, Seoul metropolitan area had less than $100Wm^{-2}$ of net radiation, which implied that radiation energy could be absorbed in the atmosphere.

• Economic and Environmental Geology
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• v.46 no.5
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• pp.469-476
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• 2013

With the advent of a new Korean government, a new national agenda (140 items) was reported. Additionally, Work Plans of the executive bodies were also released, including the Ministry of Science, ICT and Future Planning (MSIP); the Ministry of Trade, Industry and Energy (MOTIE); the Ministry of Oceans and Fisheries (MOF); and the Ministry of Environment (MOE); the Ministry of Land, Infrastructure, and Transport (MOLIT). For government-supported research institutes, it will be important to watch and analyze the changes in the government's primary policies and strategic goals. In this paper, we analyzed new governmental research and development (R&D) policy with respect to geoscience and mineral resources technology. The results indicated that the executive bodies emphasized, for the job creating 'creative economy' and the 'safety and integration society', the establishment of the creative economy; science and technology development based on creativity and innovation; creation of new growth engines by fusion and diffusion; construction of stable energy systems; promotion of environmental industry; and creating satisfactory land services. In the area of geoscience and mineral resources technology, it is time to search for a new, creative, and interagency fusion R&D contents to meet the needs of the public and boost its national competitiveness.

• Journal of Life Science
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• v.17 no.9 s.89
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• pp.1177-1181
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• 2007

Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates diverse cellular functions by modulating the activity of a variety CaM-binding proteins (CaMBPs). Because eukaryotes have multiple CaMBPs, it is important to isolate and characterize them in different tissues and conditions. So far a number of CaMBPs have been identified through classical screening methods. Many classes of proteins have been predicted to bind CaMs based on their structural homology with already known targets. In an effort to develop a method for large-scale analysis of CaMBPs in Arabidopsis, we have generated a transgenic plants overexpressing AtCaM2-GFP. We performed protein pull-down assay to test whether exogenously expressed AtCaM2-GFP proteins can interact with CaMBPs. The exogenously expressed AtCaM2-GFP could strongly interact with a CaMBP, AS1 protein. This result suggests that AtCaM2-GFP in transgenic plants may interact with many CaMBPs in plant cell. Therefore, we will be able to isolate kinds of CaMBPs by using these transgenic plants in many different tissue and environments.

• Journal of radiological science and technology
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• v.34 no.2
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• pp.97-103
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• 2011

The Full-length examination of the femur sometimes does not show all of the femur because of the limited width in collimator and size of detectors although it is located in the diagonal direction. Aim of this study is to identify usefulness of the method of combining images which obtained from two radiographic images containing the femoral head and condyle respectively. Our group interviewed 30 radiological technologists who used both palliative DR method and SM method in their examinations of the femur of patients who were carried to the emergency rooms in Gwang-ju city from August 2007 to November 2009. We evaluated twenty images according to the contraction of femoral neck, turning of knee joints and inclusion of the femur. The examination were performed by two radiologists and six radiological technologists who have more than ten years of career. The results are as follows: SM method was graded with higher score for examination time, number of examiners, emaciation, retake and patients' discomfort (p< 0.001) while the. SM method was scored higher than the palliative DR method in the palliative DR method for examination of long bone.

• Advances in Traditional Medicine
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• v.8 no.2
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• pp.154-163
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• 2008

The methanol extract of stem barks of Careya arborea Roxb. (MECA) (Family- Myrtaceae) was evaluated for antitumor activity and antioxidant status against Ehrlich's Ascites Carcinoma (EAC) bearing Swiss albino mice. After 24 h of tumor inoculation the MECA was administered at the doses of 50, 100 and 200 mg/kg body weight/mice/day for 14 days. After the last dose and 18 h fasting mice were sacrificed. The effect of MECA on the growth of transplantable murine tumor, life span of EAC bearing hosts, hematological profiles, serum and liver biochemical parameters were estimated. The MECA showed significant (P < 0.01) decrease in ascites volume, packed cell volume and viable cell count and prolonged the life span of EAC tumor bearing mice. Hematological profiles reverted to more or less normal levels in extract treated mice. The MECA also produced protective effect by decreasing the activity of serum enzymes, bilirubin and increase the protein and uric acid levels. MECA significantly (P < 0.05) decreased the levels of lipid peroxidation, while significantly (P < 0.05) increased the levels of glutathione content, vitamin C, vitamin E, superoxide dismutase and catalase CAT. The results indicate that MECA exhibited significant antitumor and antioxidant activity in EAC bearing mice.

• Advances in Traditional Medicine
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• v.8 no.3
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• pp.228-235
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• 2008

This study was conducted to evaluate the estrogen activity of silkworm (Bombyx mori) pupa extracts and their fractions. Powdered samples of freeze-dried silkworm pupa were extracted at room temperature (RT), $40^{\circ}C$, $60^{\circ}C$, $80^{\circ}C$, and $100^{\circ}C$ in water (D.W), chloroform, ethyl acetate, and methanol for 6h and then filtered (0.45 um). The extracts were then freeze-dried. The estrogenic activity of these extracts was then investigated by competition binding assays using estrogen receptor ${\alpha}\;(ER{\alpha})$ and $ER{\beta}$, and by evaluating their effects on the proliferation of the human breast cancer cell line, MCF-7. Among the extracts evaluated, water extracts prepared at RT showed the highest binding affinity to $ER{\alpha}$ ($IC_{50}$, 1.76 ug/ml) and $ER{\beta}$ ($IC_{50}$, 0.07 ug/ml). In addition, MCF-7 cells that were treated with 62.5 ug/ml of the RT extract showed the greatest increase in proliferation (2-fold; 1291.79%) when compared to control cells (659.82%). Next, the water extract that was prepared at RT (sample 1) was dissolved in D.W. and further fractionated using a Dowex 50W - 8X ($H^+$) column. The flow-through and wash were then pooled together and freeze-dried (sample 2). The bound materials were then eluted with 20 mM NaCl, after which they were applied to a Dowex 1X2 - 200 ($Cl^-$) column and washed with D.W. to remove the sodium ions. The eluants were then freeze-dried (sample 3). Of these fractions, sample 2 showed the highest binding affinity to ER{\alpha} ($IC_{50}$, 1.44 ug/ml) and $ER{\beta}$ ($IC_{50}$, 1.18 ug/ml). In addition, MCF-7 cells that were treated with sample 2 (15.6 ug/ml) showed the largest increase in growth (1159.39%) when compared to control cells (525.26%). Taken together, these results suggest that the fraction of the RT water extract of silkworm pupa referred to as sample 2 may be useful as a phytoestrogen.

• Journal of KIISE:Software and Applications
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• v.36 no.9
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• pp.732-748
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• 2009

The technology of Semantic Web realizes the base technology for context-awareness that creates new services by dynamically and flexibly combining various resources (people, concepts, etc). According to the realization of ubiquitous computing technology, many researchers are currently working for the embodiment of web service. However, most studies of them bring about the only predefined results those are limited to the initial description by service designer. In this paper, we propose a new service supporting model to provide an automatic method for plan related tasks which achieve goal state from initial state. The inputs on an planner are intial and goal descriptions which are mapped to the current situation and to the user request respectively. The idea of the method is to infer context from world model by DL-based ontology reasoning using OWL domain ontology. The context guide services to be loaded into planner. Then, the planner searches and plans at least one service to satisfy the goal state from initial state. This is STRIPS-style backward planner, and combine OWL-S services based on AI planning theory that enabling reduced search scope of huge web-service space. Also, when feasible service do not find using pattern matching, we give user alternative services through DL-based semantic searching. The experimental result demonstrates a new possibility for realizing dynamic service modeler, compared to OWLS-XPlan, which has been known as an effective application for service composition.