• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.22 no.6
• /
• pp.542-549
• /
• 2021

The purpose of this study was to provide an economical and easy preparation method for recombinant human epidermal growth factor (rhEGF) without the need for an expensive enzyme to cleave the fusion part. However, the N-terminal fusion part is still useful for affinity chromatography. The hEGF is an important hormone in cell growth and proliferation in humans, and many studies on the expression and purification of this protein have been reported. In the present study, the hEGF gene was designed to be optimized with the E. coli codon usage preference and to contain Asn-Gly at the N-terminus of the protein. The gene was inserted into pRSET_A, an E. coli expression vector, and transformed into E. coli BL21 (DE3). The recombinant fusion protein was successfully co-expressed with pG-Tf2, a chaperone vector, in E. coli and purified by Ni-NTA column chromatography. The rhEGF was then released by hydroxylamine treatment and confirmed by SDS-PAGE. ELISA analysis showed that the activity of the free rhEGF was more than 92% similar to that of commercial EGF. The biological activity of the rhEGF was confirmed by a cell proliferation test with human skin fibroblasts.

• Korea Science and Art Forum
• /
• v.30
• /
• pp.251-261
• /
• 2017

Metal is a material that has exerted a lot of influence on the development of human cultures, and has closely connected with our life from the past to the present. Types of metal we have used from the prehistoric times are varied, and iron relics take the largest percentage of metal relics excavated in our country. The biggest threat to the existence of iron relics ranging from excavated relics to the ones that are transmitted is the process of corrosion, and physical removal has been used the most for removing corroded oxides. For details for removal of corrosion oxides, this thesis aimed to research on the chemical corrosion oxides remover that protects parent material of iron relics but treats corrosion oxides only. For safe and effective removal of corrosion oxides of iron relics, this study was conducted aiming at finding the possibility of and optimized composition for removal of iron relics corrosion oxides by manufacturing new acid, alkaline and neutral oxides removers and changing their composition variously, exploring the possibility by applying the agents to modern relics. The results of this study are as follows: First, the acid solution removed only some part of corrosive substance oxidized on the surface of metal specimen. Second, the application of each of alkaline and neutral solution resulted in remaining black-colored corrosive substance, but it was removed when the quantity of the solution and the duration of application are increased. Third, All the three solutions did not cause any damage to parent material in the course of application, and showed the result that they are capable of removing unstable oxide layer while protecting parent material and stable corrosive layer as the solutions would be able to deal with situation by a relic only through the control of concentration of solution and duration of application.

• Journal of rehabilitation welfare engineering & assistive technology
• /
• v.10 no.1
• /
• pp.29-35
• /
• 2016

This paper presents an analysis of the short-range radar noise adjusting the angle of the radar reflector for the handicapped aids and suggests a complementary filter that combines a moving average filter with a heuristic algorithm to remove the radar noise. It also shows the effectiveness of the suggested filter by comparing the experiments results between the Kalman filter and the suggested filter.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2012.11a
• /
• pp.543-544
• /
• 2012

객체 추적 및 모니터링 시스템에서 안개와 같은 환경적 불완전 요소는 비전 처리 성능에 많은 영향을 준다. 특히 안개는 빛의 산란과 흡수에 의한 감쇠로 탐지 영역내의 물체의 색상이 비슷해지고 채도가 매우 떨어지게 되어 객체의 형태를 구별하기 어려워진다. 따라서 실외에서 신뢰할 수 있는 비전 처리를 위해서는 안개와 같은 환경적 불완전 요소의 제거가 필수라 할 수 있다.

• Journal of Urban Science
• /
• v.9 no.2
• /
• pp.13-20
• /
• 2020

The Recently, several countries have been affected by respiratory diseases, resulting in renewed research interest in their prevention and control. One such example was the 2015 outbreak of Middle East Respiratory Syndrome (MERS) in South Korea and COVID-19. In this study, we performed experiments and simulations based on concentration decay using CO2 as the tracer gas to elucidate the pollutant-removal efficiency for different inlet and exhaust locations and outdoor air-supply ratios. The wall inlet exhibited a higher pollutant-removal efficiency, owing to the upward movement of the air from the lower zone to the upper one. In conclusion, it is recommended that a total air-conditioning plan for isolation rooms be established as well as efficient system operation for pollutant removal and air-flow control to prevent the transmission of infections from the patients to others.

• Proceedings of the Korean Society of Computer Information Conference
• /
• 2023.07a
• /
• pp.573-574
• /
• 2023

화염 영역 검출을 위해 이전 기법에서는 화재 이미지에서 연기제거 및 색상보정을 통해 이미지를 전처리하였다. 그러나 이 기법은 임계값에 영향을 많이 받고, 밝기채널을 이용하여 검출하기 때문에 밤에 일어난 화재 이미지에서는 평균이상의 퍼포먼스를 수행하지만, 주변이 밝은 대낮의 화재 이미지에서는 퍼포먼스가 줄어드는 문제가 있다. 이를 보완하고자 본 논문에서는 CycleGAN을 이용하여 낮 이미지를 밤 이미지로 바꾸어 이미지 전처리를 진행하는 기법을 제안함으로써 화염 감지의 정확도가 개선되었음을 실험을 통해 보여준다.

• Journal of the Korea Convergence Society
• /
• v.10 no.9
• /
• pp.69-74
• /
• 2019

The purpose of this study is to compare the efficiency of bacteria removal of three different types of hand washing methods. This study performed a convenient sampling of 30 volunteers in cross-over design. The study divided the 30 volunteers into three random groups. The three groups were asked to use antiseptic soap, alcohol-based hand disinfectant, and disposable wet wipes respectively. The result of the study showed that mean log reduction values after each had washing method were $-0.45({\pm}0.69)$ with antiseptic soap and water, $-1.19({\pm}0.52)$ with alcohol-based hand disinfectant and $-0.75({\pm}0.58)$ with disposable wet wipes. The difference was statistically significant when using alcohol-based hand disinfectant compared to the other two methods (p=0.000). According to this study, alcohol-based hand disinfectant was the most effective product based on bacteria removal for hand washing. Advantages of using alcohol-based hand disinfectant are that it is cost-effective and easy to buy, also eco-friendly. Therefore, to prevent infectious disease, providing alcohol-based hand disinfectant to every corner of the community will be very helpful.

• Membrane Journal
• /
• v.32 no.5
• /
• pp.314-324
• /
• 2022

This study is aimed to design a membrane process that systematically removes contaminants including microplastics in sun-dried salt using a separation membrane. In this study, we selected the separation membrane material, pore size, and module suitable for the sun-dried salt fields, and proceeded with the experiments under the salt fields and laboratory conditions. A pilot plant was constructed and tested in our lab and in the actual saltern with the selected 200 kDa, 4 kDa ultrafiltration membranes, and 3 kDa nanofiltration membranes. Most of the impurities in the sea salt were 0.1 ㎛ in size, and more than 7 types of various microplastics were detected in the impurities. After that, as a result of checking the filtered water through the separation membrane process, no impurities were detected. As a result of comparing the existing sea salt component and the sea salt component prepared with separation membrane filtrate, impurities were effectively removed without change in the sea salt component.

• Proceedings of the Korea Institute of Convergence Signal Processing
• /
• 2005.11a
• /
• pp.167-170
• /
• 2005

Recently, filtering methods for attenuating noise while preserving image details are in progress actively. And SM(standard median) filter showed a great performance for noise removal in impulse noise environment but, it caused edge cancellation error. So, variable methods that modified SM(standard median) filter have been proposed, and CWM(center weighted median) filter is representative. Also, there are several methods to improve the efficiency based on min/max operation in term of preserving detail and filtering speed. In this paper, we managed a pixel corrupted by impulsive noise using min/max value of the surrounding band enclosing a pixel, and compared the efficiency with exiting methods in the simulation.

• Proceedings of the Korea Institute of Convergence Signal Processing
• /
• 2005.11a
• /
• pp.278-281
• /
• 2005

본 논문에서는 백색 잡음이 첨가된 오디오 신호로부터 잡음을 추정하고 이에 따른 지각 필터 보정을 통한 적응적 지각 필터의 속도 개선 알고리즘을 제안한다. 제안된 잡음 추정 알고리즘은 고주파 구간에서 획득한 잡음 에너지를 사용하여 지각필터를 구성하고 일정 대역에서 추정된 기울기를 고주파 영역에 적용함으로써 전 대역의 잡음 에너지를 효과적으로 제거할 수 있게 되며 필터의 속도 향상에도 크게 기여하게 된다. 이는 기존의 적응지각필터와의 비교를 위하여 수행속도 및 SSNR, NMR 비교를 수행하였고 그 결과를 통하여 성능 개선을 확인할 수 있었다.