• Korean Journal of Plant Taxonomy
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• v.44 no.4
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• pp.250-256
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• 2014

This study was carried out to understand the genetic relationship of three Aloe spp. cultivated in Korea, A. saponaria, A. vera and A. arborescens and a new variant in Korea based on three plastid (matK, trnL-F, rbcL) and one nuclear (ITS regions) DNA barcode markers. A total of 2,420 bp sequence was amplified. Two indels were detected in the trnL region, and also several species specific nucleotide loci were detected in all 29 parsimonious informative sites, and 148 variable sites were detected among four taxa studied while 170 variable and 75 parsimonious sites were detected when other Aloe spp. in worldwide were used. An UPGMA phenogram with 10,000 bootstrap replication showed that the new variant was closest to A. vera. The variant was not morphologically and genetically concurrent with any reported species so far. The clustering of Aloe species were broadly in agreement with previously reported results.

• Korean Journal of Plant Taxonomy
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• v.41 no.4
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• pp.315-323
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• 2011

To elucidate the taxonomic status of the genus Megaleranthis Ohwi, a monotype in Korea, its distribution and morphological characteristics were studied and a cluster analysis was performed. The cluster analysis showed that Megaleranthis was separately clustered from Trollius and Calathodes. Megaleranthis is morphologically different from Calathodes Hook.f. et Thomson in having petals, and from Trollius L. in having an involucre instead of a caulescent leaf under the flower and follicles with simple veins. The three genera above are distributed independently within Asia, although they can be found together at the boundaries where their distributions overlap: Trollius and Calathodes occur together between the Sichuan and Yunnan provinces in China, and Megaleranthis and Trollius can both be found on the northern edge of the Korean Peninsula. From both a morphological and a distributional viewpoint, Megaleranthis should be recognized as an independent genus different from both Trollius and Calathodes.

• Korean Journal of Plant Taxonomy
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• v.41 no.2
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• pp.119-129
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• 2011

Phylogenetic studies were conducted to evaluate the taxonomic relationships among 27 taxa, including 2 outgroups of Korean Campanulaceae, using PCR-RFLP analysis and ITS sequences. In the PCR-RFLP analysis, 15 restriction endonucleases produced 244 restriction sites and size variations from the chloroplast DNA, and 59 restriction sites (24%) showed polymorphism. The length of the ITS regions ranged from 588 bp to 797 bp. The sequence divergence including the outgroups is 0-39.36%. Phylogenetic analyses based on PCR-RFLP and ITS data suggest that Campanulaceae is monophyletic; Codonopsis and Platycodon forms an independent clade; the Peracarpa and Asyneuma clade is a sister to the Adenophora-Hanabusaya clade; Campanula is monophyletic; and Wahlenbergia basally branches within the ITS tree, whereas they are placed between Campanula and the Codonopsis-Platycodon clade in the PCR-RFLP tree; Hanabusaya is placed within the Adenophora clade; and Adenophora is paraphyletic and shows discordance to the infrageneric classifications based on morphological data. The present results show two data sets, largely congruent at the generic level, but their phylogenetic positions, in particular the Wahlenbergia and Hanabusaya and the infrageneric classifications in Adenophora, show some incongruence.

• Korean Journal of Plant Taxonomy
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• v.35 no.3
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• pp.161-174
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• 2005

In order to presume the relationships between two species of P. ternata and P. tripartia, and their populations of the Korean Pinellia, RAPD analysis was performed. The length of the amplified DNA fragments ranged from 300 to 2,500bp. Seventy scorable RAPD makers were found from the PCR reactions with 7 random oligoprimers and were analyzed by Nei-Li's genetic coefficient. Also, some regional groups instead of same taxa were clustered from the phenogram of UPGMA analysis and NJ tree. Populations within each species were clustered at low genetic distance, there had the closed relationship. According to the regional individuals, Pinellia ternata was showed the variation pattern of morphological (leaf shape and flower color) and cytological characters(somatic chromosome numbers). So we suggested to difference of characteristic variety based on variety of habitat. According to this study, new species (Pinellia sp.) was affiliated with Pinellia and had the closest relationship with Hallasan and Japan population. The RAPD data was very useful to define the genetic variation and to discuss the relationships among the intraspecific taxa and their populations of the Korean Pinellia.

• Journal of Korean Society of Forest Science
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• v.82 no.2
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• pp.166-175
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• 1993

The genetic variation patterns at 23 loci coding for 16 isozymes in eight natural populations of Pinus densiflora for. erects distributed in Kangwon-Kyungbuk region and 17 populations of Pinus densiflora and 13 populations of Pinus thunbergii were compared. The absence of marker alleles specific to P. thunbergii and almost the same allele-frequency distributions to those of P. densiflora did not support the hypothesis that P. densiflora for. erecta is a introgressive hybrid between P. densiflora and P. thunbergii. From the results of the hierarchial analysis of population differentiation using Wright's F statistics(1978), the frequency distributions of single-locus distance coefficients and other genetic analysis (genetic distance, cluster analysis, factor analysis, resin duct analysis), it was concluded that Pinus densiflora for. erecta cannot be treated genetically as a distinct group from other natural populations of P. densiflora.

• Korean Journal of Plant Taxonomy
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• v.34 no.3
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• pp.205-220
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• 2004

Numerical analysis based on the 67 morphological characters from 28 populations of 6 species of Korean Rhus sensu lato (Anacardiaceae) was performed for the taxonomic delimitation. Based on the results of PCA with 47 quantitative characters, the sum of contributions for the total variance of three major principal components was 77,9% (PCl 35.2%, PC2 22.5% and PC3 20.2%). The sum of contributions for the total variance of three major principal components were 90,7% (PCl 37.7%, PC2 33.0% and PC3 20.0%) based on the results of PCA with 20 qualitative The characters. Two dimensional plotting from PCA results recognized six distinct species. UPGMA phenogram based on simple matching coefficient method recognized clear taxonomic delimitations among six taxa. On the cluster analysis, qualitative characters were more useful for grouping the species treated. Numerical analysis was very valuable to delimit the Korean taxa of Rhus s.l.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2003.10a
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• pp.71-74
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• 2003

주광성이 있는 어족을 밀집시켜 어획하기 위하여 사용되는 집어등은 어류 중 불빛에 유집이 잘 되는 멸치, 오징어 및 꽁치 둥을 대상으로 하는 어업에서 주로 사용된다. 어장에 분산되어 있는 어류에 집어등을 투사하면, 농밀하게 어군을 형성하게 되므로 효과적인 어획이 가능하게 된다. 본 연구에서는 꽁치봉수망 조업시 어선의 집어등에 의한 수중 환경의 변화를 구명하여 광환경이 꽁치의 어획에 미치는 영향을 분석하기 위한 기초 단계로 꽁치봉수망 어선의 집어등 수중 조도를 측정하여 꽁치가 분포하는 어획수심의 광환경을 분석하였다. (중략)

• Applied Microscopy
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• v.32 no.4
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• pp.345-360
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• 2002

Pollen morphology of four genera, 22 species of Korean Crassulaceae has been examined with light and scanning electron microscopy. The presence/absence of the aperture margo and granules was useful characters to clarify the generic boundaries, and the shapes of the streate were significant to delineate taxonomic relationships within the genus Sedum. The pollen grains of Korean Crassulaceae were small and medium in size. Hylotelephium viviparum has the smallest pollen grains, and H. viridescens the largest. The pollen grains are tricolporate, circular in polar view and were prolate to prolate-spheroidal in equatorial shape. Korean Crassulaceae species have striate grains. The close pollen morphological relationships among Hylotelephium, Meterostachys and Orostachys species were concordant to the results based on the previous molecular and morphological studies. The results of the clustering analysis using pollen characters support the segregation of Sedum sensu lato which has been proposed by the previous authors. Within the genus Sedum, S. kamtschatium is closely related to S. middendorffianum with granules on the surface of the pollen grains. Sedum polytrichoides, a member of the Sedum, was clustered to the species of the Meterostachys, Hylotelephium and Orostachys, and needs to reexamine the taxonomic status. O. malacophyllus collected at Chungdong-Jin was significantly differ from the same species from Gampo in pollen and morphological characters.

• Korean Journal of Plant Resources
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• v.27 no.4
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• pp.337-343
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• 2014

This study was conducted to classify the variation in leaf and fruit morphology among natural grown 16 Cudrania tricuspidata stands. As results of the analysis in C. tricuspidata stands total 134 Species were found and as results of the analysis in leaf morphology, it was grouped as Group IV. By the analysis of variance in length, width, and weight of fruits were differed in 15 C. tricuspidata stands. The length, width, and weight of fruits were higher in Jinan and Sunchang, than Gochang, Gimje, and Goheung. By the cluster analysis of 3 fruit morphology were classified as 3 Groups. According to the results of this study C. tricuspidata leaves and fruit morphological characteristics for each group were compared by analysis there were differences between the morphological characteristics and the characteristics of the fruit did not match.

• Korean Journal of Plant Resources
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• v.20 no.4
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• pp.272-280
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• 2007

In order to presume the genetic relationship about two species and their 30 regional populations of the Saururus and Houttuynia of Saururaceae, RAPD analyses were performed. The length of amplified DNA fragments ranged from 300 to 2,000 bp. 156 scorable RAPD makers were found from PCR reactions with sixteen random oligoprimers. Also, some regional populations were clustered separately from the UPGMA phenogram. The OTUs between cultivated and natural populations were distinguished distinctly on the UPGMA phenogram. And the regionals populations of the treated taxa were clustered. Among the regional populations of two species, GN populations had the close relationship JJ populations rather than JN populations. The RAPD data was very useful to define the genetic relationship and distinguish the cultivated populations from natural populations by regional distributions in this study.