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Analysis of phylogenetic relationship among Korean Pinellia Tenore (Araceae) using RAPD markers  

Tae, Kyoung-Hwan (Department of Life Science, Daejeon University)
Kim, Dong-Kap (Department of Life Science, Daejeon University)
Kim, Joo-Hwan (Department of Life Science, Daejeon University)
Publication Information
Korean Journal of Plant Taxonomy / v.35, no.3, 2005 , pp. 161-174 More about this Journal
Abstract
In order to presume the relationships between two species of P. ternata and P. tripartia, and their populations of the Korean Pinellia, RAPD analysis was performed. The length of the amplified DNA fragments ranged from 300 to 2,500bp. Seventy scorable RAPD makers were found from the PCR reactions with 7 random oligoprimers and were analyzed by Nei-Li's genetic coefficient. Also, some regional groups instead of same taxa were clustered from the phenogram of UPGMA analysis and NJ tree. Populations within each species were clustered at low genetic distance, there had the closed relationship. According to the regional individuals, Pinellia ternata was showed the variation pattern of morphological (leaf shape and flower color) and cytological characters(somatic chromosome numbers). So we suggested to difference of characteristic variety based on variety of habitat. According to this study, new species (Pinellia sp.) was affiliated with Pinellia and had the closest relationship with Hallasan and Japan population. The RAPD data was very useful to define the genetic variation and to discuss the relationships among the intraspecific taxa and their populations of the Korean Pinellia.
Keywords
genetic distance; NJ tree; Pinellia; RAPD; relationship;
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Times Cited By KSCI : 2  (Citation Analysis)
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