• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.9-9
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• 2023

한국 특산 식물인 미선나무 (Abeliophyllum distichum)는 IUCN Red List의 EN (위기종) 등급에 지정되 어 보호받아 왔다. 미선나무는 국내 자생지 6곳을 포함하는 13개의 서식지에서 생육되고 있다고 알려져 있다. 2017년 이후, 국내에서는 지속적인 보전과 관리하에 법정보호종에서 해제되어 멸종위기종 복원 성 공 사례의 귀감이 되었으나 국내를 제외하면 미선나무의 활용은 아직 어려운 현실이다. 미선나무는 1속 1종이지만 종 내에서 다양성이 존재한다. 꽃의 형태적 특징에 따른 다양한 분류가 이루어지며, 옥황1호을 포함한 분홍, 상아, 연녹색, 흰색 등으로 구분된다. 유전체 연구 및 성분 분석을 통해, 각 표현형마다 유전 정보와 유효 성분의 차이가 존재한다는 것이 알려졌다. 활용 가치를 높이려면, 유전적으로 안정되고 표준화가 가능한 원료가 필요하며, 이는 신품종을 이용하거나 새로운 개발이 필요하다는 것을 내포한다. 일반 식품으로의 활용은 어려우나 기능성 화장품의 원료 등으로 활용되고 있으며, 이러한 활용들은 미선나무의 높은 활용 가치를 투영한다. 활용 가치 평가를 위해 수행된 DNA 손상, 멜라닌 생합성, 항염증, 항산화, 항암 억제 등의 연구를 통해 다양한 약리학적 효능을 가진다는 결과들이 도출되었고, 많은 문헌들이 그 근거를 뒷받침한다. 또한, 유효 성분의 합성경로를 재정립하고 RNA 분석을 통해 그 양상을 예측하기 위한 연구가 진행 중에 있다. 미선나무에서 유래한 식물조직 배양체인 callus의 유도 및 배양이 진행되고 있으며, 옥황1호 유래 callus를 활용하여 다양한 연구를 수행하고 있다. 앞서 언급한 미선나무 연구 및 개발에 대한 한계를 파악하고, 천연 식물 자원으로의 미선나무에 대한 지속적인 관심과 지원을 통해 나고야의정서에 대응하는 전략적 접근이 필요하다.

• Korean Journal of Ichthyology
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• v.32 no.2
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• pp.39-48
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• 2020

The slender shinner (Pseudopungtungia tenuicorpa), a tiny freshwater fish of about 8 to 10 cm belonging to Cyprinidae, is an endangered species found only in the Han and Imjin Rivers on the Korean Peninsula. During the breeding season, this species spawns in nests of Coreoperca herzi, a predator of this species, or small crevices on rocks. This unique reproductive ecology can make this species more vulnerable to anthropogenic perturbance that can further limit the places to spawn. Here, mtDNA and microsatellite loci were analyzed to identify the genetic diversity and structure of slender shinners and further to provide the basic data necessary for the conservation planning of this species. A total of 28 polymorphic microsatellite markers were developed using Illumina paired-end sequencing, and 67 slender shinners collected from three localities in the Han River were genotyped using these loci. This species showed a remarkably high level of genetic diversity with mean expected heterozygosity of 0.914 and mean allele number per locus of 27.9, and no signature of drastic demographic decline was detected. As a result of our microsatellite analysis, the genetic structure between the two stems of the Han River, North Han and South Han, was prominent. Such a genetic structure was also evident in the sequence analysis of 14 haplotypes obtained from mtDNA control region. Although slender shinners are only found in very limited areas around the world, the genetic structure indicates that there is a block of gene flow among the populations, which should be reviewed in the future if management and restoration of this species is needed.

• Journal of Life Science
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• v.23 no.8
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• pp.963-969
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• 2013

Although it is popularly believed that vitamin C protects cells from various genotoxicants, the degrees and mechanisms of itsprotective actions are not fully understood. In this study, vitamin C's protective effects against various genotoxicants were quantified, together with subsequent analyses on the mechanisms of these protective effects. Comet assay was employed to measure the degree of DNA damage in Chinese hamster ovary cells (CHO-K1) exposed to five genotoxicants, $H_2O_2$, $HgCl_2$, N-methyl-N-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline-1-oxide (4NQO), and UV-irradiation. In cases cells were treated with $H_2O_2$, $HgCl_2$, and 4NQO together with vitamin C, the damage to DNA decreased to the level of the control group. In cases of UV-irradiation, the protective effect of vitamin C appeared, but did not reach the control levels. Interestingly, vitamin C did not have protective effects against the genotoxicity of MNNG. The degrees of DNA damage of cells treated with vitamin C prior to exposure togenotoxicants were 28~49% lower than those of cells treated with vitamin C after being exposed to genotoxicants. In conclusion, vitamin C had strong antioxidanteffects against genotoxicants by being a primary antioxidant blocking genotoxicity reaching the cells, rather than being a secondary antioxidant acting on post-exposure DNA repair processes. However, vitamin C's protective effects appearto be limited, as there are genotoxicants, such as MNNG, whosegenotoxicityis not affected by vitamin C. Therefore, the results of this study warrant furtherstudies on toxic mechanisms of genotoxicants and their interactions with protective mechanisms of vitamin C.

• Korean Journal of Ichthyology
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• v.32 no.3
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• pp.117-129
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• 2020

The body color pattern in fish is a distinctive feature for species identification. The blass bloched rockfish Sebastes pachycephalus is a commercially important marine fish species, distributed in the central and southern parts of Korea and south Hokkaido of Japan. It has a morphological feature divided into four subspecies according to with or lacking distinct spots on the body surface, and to the location of markings on the body surface. However, the genetic basis of body color pattern of S. pachycephalus is still unknown. Thus we analyzed the transcriptome of S. pachycephalus skin samples using RNA-seq analysis to investigate functional genes related to body color patterns. The experimental skin samples were prepared by classified into 'Wild type' (lacking distinct spots and markings) and 'Color type' (with distinct spots and marking). Two skin sample transcriptomes were compared pairwise and the results revealed that were 164 differentially expressed unigenes in the skin samples of 'Wild type' and 'Color type'. Gene Ontology analysis of 164 differentially expressed unigenes showed that these genes were included in the functional group of molecular function (2 genes), biological process (46 genes), and cellular component (6 genes). There were several genes that body color type skin specific expression and the genes were CTL (Galactose-specific lectin nattectin), CUL1 (Cullin-1), CMAS (N-acylneuraminate cytidylyltransferase), NMRK2 (Nicotinamide riboside kinase 2), ALOXE3 (Hydroperoxide isomerase ALOXE3), SLC4A7 (sodium bicarbonate cotransporter 3). Our study is the first attempt to search for functional genes involved in the formation of body color patterns in S. pachycephalus. The differentially expressed unigenes obtained in this study can be used as candidate genes for functional gene study related to body coloration of fish.

• Journal of Scientific & Technological Knowledge Infrastructure
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• s.3
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• pp.20-25
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• 2000

생물정보학은 넓은 의미로 컴퓨터를 이용하여 모든 생화학정보를 광범위하게 연구.활용하는 학문이라고 할 수 있다. 하지만 최근의 인간유전체사업(Human Genome Project)의 인기에 의해 생물정보학이 DNA나 단백질의 서열 정보 분석의 분야로만 편중되어 의미되는 경향이 많은 것이 사실이다.

• Journal of Scientific & Technological Knowledge Infrastructure
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• s.3
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• pp.68-75
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• 2000

생물정보학은 넓은 의미로 컴퓨터를 이용해 모든 생화학정보를 광범위하게 연구.활용하는 학문이라고 할 수 있다. 하지만 최근의 인간유전체사업(Human Genome Project)의 인기에 의해 생물정보학이 DNA나 단백질 서열정보 분석의 분야로만 편중되어 의미되는 경향이 많은 것이 사실이다.

• Communications of the Korean Institute of Information Scientists and Engineers
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• v.34 no.2
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• pp.43-56
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• 2016

• Proceedings of the Korean Vacuum Society Conference
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• 2000.02a
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• pp.68-68
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• 2000

펄스레이저 증착법(이하 PLD)을 이용하여 마이크로파 유전체 소자 및 절연 산화막으로의 응용을 위한 MgTiO3 박막을 다양한 기판상에서 증착하였다. 사파이어 기판에 (a,c-plane Al2O3) 성장된 MgTiO3 박막은 에피텍셜 성장(epitaxial growth)이 되었으며, SiO2/Si 및 Pt/Ti/Si 기판위세 성장된 MgTiO3 박막의 경우 003방향으로 배향(oriented) 되었다. MgTiO3 박막은 450~75$0^{\circ}C$까지 기판온도를 변화시키면서 증착시켰으며, 증착시 산소분압은 50~200 mTorr로 변화시켰다. PLD 증착시 타켓에 조사된 레이저 에너지 밀도는 약 2J/cm2였으며, MgTiO3 박막 증착후 200Torr O2 분위기에서 상온까지 1$0^{\circ}C$/min 의 속도로 냉각시켰다. 사파이어 c-plane 상에서 일머나잇(ilminite) MgTiO3 구조가 55$0^{\circ}C$ 에피텍셜 성장하는 것을 관찰할 수 있었으며, 사파이어 a-plane 상에서는 MgTiO3 구조가 $650^{\circ}C$ 이상부터 110방향으로 배향되며 성장하였다. $600^{\circ}C$ 이상에서 c-축으로 배향된 구조를 갖고 있었다. 증착된 MgTiO3 박막의 조성분석(stoichio metric analysis)을 위해 RBS 분석을 수행하여, 증착에 이용된 타켓과 동일한 조성을 갖는 MgTiO3 박막이 성장된 것을 확인할 수 있었다. 사파이어 기판상에 증착된 MgTiO3 박막은 가시영역에서 투명하였으며, 약 270nm 파장을 갖는 영역에서 급격한 흡수단을 보였다. 이때의 MgTiO3 박막은 AFM 분석을 통해 약 0.87mn rms roughness 값을 갖는 매우 평탄한 표면구조를 갖고 있는 것을 확인하였다. MIM(Pt/MgTiO3/Pt) 구조의 캐패시터를 형성시켜 MgTiO3 박막의 유전특성(dielectric properties)을 관찰하였다. PLD로 성장된 MgTiO3 박막의 유전율(relative dielectric constant)은 약 22였으며, 1MHz에서 약 1.5%의 유전손실(dielectirc loss) 값을 보였다. 또한 이때 MgTiO3 박막은 낮은 유전분산값을 보였다.

• Korean Journal of Poultry Science
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• v.32 no.1
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• pp.29-34
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• 2005

This study was conducted to estimate the effects of genotype for chicken major histocompatibility complex (MHC) B-LB genes on economic traits. To detect polymorphism, 400 bp fragments of MHC B-LB genes were obtained and sequenced. After digestions using restriction enzyme Hea III, two restriction enzyme sites were observed. There were two mutations at position 427 and 651 those were decided as Type I and Type II, respectively. Using RFLP analyses, type I were genotyped to TT, TC and CC, and type II to MM, Mm and mm. The relatively higher TC genotype frequencies (0.8) of Type I and Mm genotype frequencies (0.88) of Type II were observed in Korean native chickens. The effects of the genotype on 150 days body weight trait were investigated by the associations of CC and Mm genotypes (P<0.05) in Korean native chickens. This result suggests that a significant association exists between the SNP and 150 days body weight.

• Journal of Life Science
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• v.12 no.6
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• pp.715-720
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• 2002

A mutant Arabidopsis thaliana which is very sensitive to Ultraviolet-B(UV-B) radiation has been isolated by ethylmethane sulfonate(EMS) mutagenesis. Genetic cross proved the UV-sensitive gene(uvs) to segregate as a single Mendelian locus. For mapping of uvs, we crossed Arabidopsis thaliana Lansberg with uvs plant(Columbia), and made F2 plants by F1 selfcross. We designed 10 kinds of CAPS marker primers. Each primers amplifies a single mapped DNA sequence from uvs and Lansberg erecta ecotyres. Also identified was at least one restriction endonuclase for each of these PCR product that generates ecotype-specific digestion pattern. We got crossing over value of UB-sensitivity and each CAPS marker which located on different chromosome arm. The value of crossing over showed that uvs was linked to LFY3 which was on chromosome 5.