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http://dx.doi.org/10.5352/JLS.2013.23.8.963

Protective Effects of Vitamin C against Genomic DNA Damage Caused by Genotoxicants  

Yu, Gyeong Jin (Department of Biology, Kyungsung University)
Lee, Chun Bok (Department of Biology, Kyungsung University)
Publication Information
Journal of Life Science / v.23, no.8, 2013 , pp. 963-969 More about this Journal
Abstract
Although it is popularly believed that vitamin C protects cells from various genotoxicants, the degrees and mechanisms of itsprotective actions are not fully understood. In this study, vitamin C's protective effects against various genotoxicants were quantified, together with subsequent analyses on the mechanisms of these protective effects. Comet assay was employed to measure the degree of DNA damage in Chinese hamster ovary cells (CHO-K1) exposed to five genotoxicants, $H_2O_2$, $HgCl_2$, N-methyl-N-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline-1-oxide (4NQO), and UV-irradiation. In cases cells were treated with $H_2O_2$, $HgCl_2$, and 4NQO together with vitamin C, the damage to DNA decreased to the level of the control group. In cases of UV-irradiation, the protective effect of vitamin C appeared, but did not reach the control levels. Interestingly, vitamin C did not have protective effects against the genotoxicity of MNNG. The degrees of DNA damage of cells treated with vitamin C prior to exposure togenotoxicants were 28~49% lower than those of cells treated with vitamin C after being exposed to genotoxicants. In conclusion, vitamin C had strong antioxidanteffects against genotoxicants by being a primary antioxidant blocking genotoxicity reaching the cells, rather than being a secondary antioxidant acting on post-exposure DNA repair processes. However, vitamin C's protective effects appearto be limited, as there are genotoxicants, such as MNNG, whosegenotoxicityis not affected by vitamin C. Therefore, the results of this study warrant furtherstudies on toxic mechanisms of genotoxicants and their interactions with protective mechanisms of vitamin C.
Keywords
Comet assay; DNA damage; genotoxicants; protective effect; vitamin C;
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1 Antunes, L. M. and Takahashi, C. S. 1999. Protection and induction of chromosomal damage by vitamin C in human lymphocyte cultures. Teratog Carcinog Mutagen 19, 53-59.   DOI
2 Berger, T. M., Polidori, M. C., Dabbagh, A., Evans, P. J., Halliwell, B., Morrow, J. D., Roberts, L. J. and Frei, B. 1997. Antioxidant activity of vitamin C in iron-overloaded human plasma. J Biol Chem 272, 15656-15660.   DOI   ScienceOn
3 Blair, A., Lee, S. H. and Oe, T. 2001. Vitamin C-induced decomposition of lipid hydroperoxides to endogenous genotoxins. Science 292, 2083-2086.   DOI   ScienceOn
4 Blair, A., Lee, S. H., Williams, M. V. and Dubois, R. N. 2005. Cycolooxygenase- 2-mediated DNA damage. J Biol Chem 280, 28337-28346.   DOI   ScienceOn
5 Bowman, G. L., Frei, B., Dodge, H., Calabrese, C., Oken, B. S., Kaye, J. A. and Quinn, J. F. 2009. Ascorbic acid and rates of cognitive decline in Alzheimer's disease. J Alzheimers Dis 16, 93-98.
6 Bsoul, S. A. and Terezhalmy, G. T. 2004. Vitamin C in health and disease. J Contemp Dent Pract 5, 1-14.
7 Castillo, J., Benavente-Garcia, O., Lonente, J., Alcaraz, M., Redondo, A., Ortuno, A. and Del Rio, J. A. 2000. Antioxidant activity and radioprotective effects against chromosomal damage induced in vivo by X-rays of flavan-3-ols (Procyanidins) from grape seeds (Vitis vinifera): comparative study versus other phenolic and organic compounds. J Agric Food Chem 48, 1738-1745.   DOI   ScienceOn
8 Chen, K., Suh, J., Carr, A. C., Morrow, J. D., Zind, J. and Frei, B. 2000. Vitamin C suppresses osidative lipid damage in vivo, even in the presence of iron overload. Am. J Physiol Endocrinol Metab 279, 1406-1412.
9 Eylar, E., Baez, I., Navas, J. and Mercado, C. 1996. Sustained levels of ascorbic acid are toxic and immunosuppressive for human T cells. P R Health Sci J 15, 21-26.
10 Glascott, P. A. Jr., Tsyganskaya, M., Gilfor, E., Zerm, M. A. and Farber, J. L. 1996. The antioxidant function of the physiological content of vitamin C. Mol Pharmacol 50, 994-999.
11 Halliwell, B. 1996. Vitamin C: antioxidant or pro-oxidant in vivo? Free Radic Res 25, 439-454.   DOI   ScienceOn
12 Hara, R., Mo, J. and Sancar, A. 2000. DNA damage in the nucleosome core is refractory to repair by human excision nuclease. Mol Cell Biol 20, 9173-9181.   DOI   ScienceOn
13 Kandarkar, S. V. and Sawant, S. S. 1996. The effect of vitamin C on the hamster cheek pouch treated with the water soluble carcinogen 4-nitroquinoline-1-oxide (4NQO). Eur J Cancer B Oral Oncol 32B, 230-237.
14 Kandarkar, S. V. and Reade, P. C. 1991. The effect of topical vitamin C on palatal oral mucosal carcinogenesis using 4-nitroquinoline-1-oxide. J Biol Buccale 19, 199-204.
15 Kang, S. A., Jang, Y. J. and Park, H. 1998. In vivo dual effects of vitamin C on parauat-induced lung damage: dependence on released metals from the damaged tissue. Free Radical Res 28, 93-107.   DOI   ScienceOn
16 Kosmoski, J. V. and Smerdon, M. J. 1999. Synthesis and nucleosome structure of DNA containing a UV photoproduct at a specific site. Biochemistry 38, 9485-9494.   DOI   ScienceOn
17 Nefic, H. 2001. Anticlastogenic effeft of vitamin C on cisplatin induced chromosome aberrations in human lymphocyte cultures. Mutat Res 498, 89-98.   DOI   ScienceOn
18 Ogrunc, M., Becker, D. F., Ragsdale, S. W. and Sancar, A. 1998. Nucleotide excision repair in the third kingdom. J Bacteriol 180, 5796-5798.
19 Padayatty, S. J., Katz, A., Wang, Y., Eck, P., Kwon, O., Lee, J. H., Chen, S., Corpe, C., Dutta, A., Dutta, S. K. and Levine, M. 2003. Vitamin C as an Antioxidate: Evaluation of its role in disease prevention. J Am Coll Nutr 22, 18-35.   DOI   ScienceOn
20 Piperakis, S. M., Tassiou, A. M. and Visvardis, E. E. 1997. Study of DNA damage induction and repair capacity of fresh and cryopreserved lymphocytes exposed to $H_2O_2$ and $\gamma$-irradiation with the alkaline comet assay. Mutat Res 383, 71-80.   DOI   ScienceOn
21 Rao, M. V. 1997. Mercury and its effects on mammalian systems-a critical review. Ind J Environ Toxicol 7, 3-11.
22 Sato, K., Kusaka, y., Zhang, Q., Deguchi, Y., Li, B., Okada, K., Nakakuki, K. and Muraoka, R. 1997. Direct effect of inorganic mercury on citrate uptake by isolated rat renal brush border membrane vesicles. Ind Health 35, 456-460.   DOI
23 Singh, N. P., McCoy, M. T., Tice, R. R., and Schneider, E. L. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175, 184-191.   DOI   ScienceOn
24 Sona, R. and Darina, S. 2002. Effects of vitamins C and E on cytotoxicity induced by N-nitroso compounds, N-nitrosomorpholine and N-methyl-N'-nitro-N-nitrosoguanidine in Caco-2 and V79 cell lines. Cancer Lett 182, 11-18.   DOI   ScienceOn
25 Zhou, D. F., Ke, W. Z., Chen, N. F. and Ji, K. 2005. Effects of different levels of vitamin C on UV radiation-induced DNA damage. Guang Pu Xue Yu Guang Pu Fen Xi 25, 2012-2015.