• The Korean Journal of Zoology
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• v.37 no.3
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• pp.452-465
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• 1994

미꾸리속의 미꾸리(Misgumus anguillicaudatus)와 미꾸라지(M. mizolepis)의 종내 및 종간 유전적 변이와 유전적 차이를 알아보고자 2종 15개집단과 대만산 Paramisgumus김늠bwanus 1개 집단을 대상으로 전기영동법의 의한 동위효소분석을 실시한 결과 각. ongui각caudotus 9개 집단의 평균 유전적 변이정도는 P=34.20%, Ho=0.099, He=0 114였고. M. mizolepgs 6개 집단은 평균 P=35.55%, Ho=0 141, He=0.148이었으며, p연abwonus는 P=45.9%, Ho=0.132. 연e=0.119로서 일반적인 담수어류에 비해 높은 변이를 나타내었다 종간 평균 유전적 근연치를 비교한 결과 M. aneuflISca udatuo와 M. mfzole부랍 사이는 5=0.467이었고. M. angu연흘audatus와 P. dabrvanus 사이는 5=0.475로 비교적 근연관계가 낮았으나 M. mizolep결와 P. dabwanuo 사이는 5=0.834로 매우 가까운 유연관계를 나타냈다 유전적 차이치를 토대로 M. ongu비상라udctus와 M. mfzolep결의 종분화 연대를 산출한 결과 이들은 약 350만년전에 분화된 것으로 추정되었다.

• Journal of Life Science
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• v.14 no.3
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• pp.425-428
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• 2004

Molecular authentication and genetic polymorphism of Korean ginseng cultivars and accessions were investigated using ISSR (inter-simple sequence repeat amplification) markers. Five primers among 56 produced clear and reproducible DNA fragments among seven cultivars and accessions. A total of 43 bands ranging from 250 bp to 1,700 bp from five primers were scored. Average number of bands per primer was 8.6 and only nine bands were polymorphic across the six Panax ginseng from Korea. Especially Chunpoong cultivar exhibited the highest level of polymorphism, whereas other accessions did not showed almost any polymorphism. Consequently, these ISSR markers will be available to differentiate Chunpoong cultivar from other major Korean ginseng cultivars and accessions, such as Yunpoong, Hwangsukjong and Jakyungjong, at the DNA level.

• Journal of Life Science
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• v.22 no.3
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• pp.285-292
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• 2012

Genus Spiraea is composed of many long-lived woody species that are primarily distributed throughout Asia and Europe. In this study, we evaluated a representative sample of the 38 taxa in the world, including 14 in Korea, with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within the genus. The molecular data allowed us to resolve well-supported clades in the taxa. In 47 world accessions (38 taxa: 14 Korean taxa, 33 world taxa, and 9 overlapping taxa), total alignment length was 689 positions, of which 452 were parsimony informative, 527 variable, 75 singleton, and 159 constant characters. Although the phylogenic tree showed that many taxa of genus Spiraea were well separated from each other, many branches were not congruent with the morphological characteristics and geographical distributions of the genus. There were 430 segregating sites and the nucleotide diversity (${\pi}$) value was 0.281. Under the neutral mutation hypothesis, the probability that the Tajima test statistic (D) is positive (2.325) is more than 0.5. Therefore, there may be a site at which natural selection, which increases genetic variation, is operating.

• Korean Journal of Environmental Biology
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• v.34 no.3
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• pp.169-176
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• 2016

Although chemical fertilizers have a quick effect and broad applicability to agricultural fields, they have caused many problems like increasing soil acidity or decreasing soil organic matters. Environmental-friendly agriculture has been attempted in various ways such as organic agriculture, natural farming, low input and sustainable agriculture. The common interest of all environmental-friendly systems is to decrease burden to agricultural environment by low input of agricultural labor and materials. This study was conducted to estimate overwintering capacity and genetic distance among Chinese Milk Vetch (Astragalus sinicus, CMV) collections based on morphological characteristics and AFLP (Amplified fragment length polymorphism) analysis. Furthermore, the effect of CMV as green manure was observed in mix-cultured paddy fields with rice, sesame and sweet-potato. An another objective of this study was also to compare the pattern of weed occurrence in paddy fields with or without CMV and different rice transplanting times. The CMV collected from Paju district in central region of Korea was successively occurring through self-reseeding without artificial management. However, there was no noticeable difference in growth habit between Paju native CMV and introduced CMV from China which is currently used in farm fields. On the basis of multi-dimensional scaling and tree analyses, there are no significant difference of agricultural growth characteristics among Paju and chinese collections only excepting leaf angle and root length. The flowering time of Gurye collection was fast for 1 week as compared to other collections. AFLP that was commonly used for plant classfication, was applied to exam the genetic variation of CMV collections. Total 579 PCR products and 336 polymorphic fragments were generated using 8 primer pairs.

• Korean Journal of Ecology and Environment
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• v.53 no.3
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• pp.265-274
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• 2020

Silver croaker (Pennahia argentata) is a turbulent species that is widely distributed worldwide and is mainly found in the bottom of the ocean. In the study, we characterized the cytochrome c oxidase subunit I (COI) gene of the mitochondrial DNA (mtDNA) on P. argentata inhabiting Gwangyang Bay and analyzed the phylogenetic location of marine fish species. As a result of multiple arrangement of 605 bp COI sequences, high homology of mtDNA nucleotide sequences was confirmed in the silver croakers from Gwangyang Bay (98~100%). However, the nucleotide variation was different according to the catching points of the inland and the open seas of Gwangyang Bay. The nucleotide sequence variation in COI was high in P. argentata from the open seas of Gwangyang Bay (43.2~70.3%). Furthermore, the phylogenetic analysis of 13 fish showed that P. argentata from Gwangyang Bay were grouped into one clade with P. argentata reported in Taiwan, and the evolutionary distance was 0.036. In addition, it was identified that the evolutionary distance was close to that of fish belonging to the Mi-iuy croaker (Miichthys miiuy) and the Big-head pennah croaker (Pennahia Macrocephalus) (0.041~0.048). The result of these studies will be used as the key genetic information for fisheries resources monitoring and species diversity management according to the coastal environment.

• The Korean Journal of Zoology
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• v.34 no.1
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• pp.110-122
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• 1991

기름종개과의 N. toni, L. costota, N. multifasciata 3종에 대한 형태분석, 핵형분석 및 전기영동법에 의한 유전자 분석을 실시 각 종의 지리적 변이 및 계통적 유연관계를 조사하였다. 형태 측정치에 대한 discriminant 분석결과 연 yoni 월악산집단, 1. costoto 거제도집단 그리고 환multifasc지물 산철집단이 같은 종내 타 집단들과 형태상 완전히 분리 되어졌다. 핵형분석에서 N. muftifosciota와 1. costoto는 2n: 50으로 염색체수는 조사된 전 집단이 변리없이 동일하였다. N to티 삼척과 진부집단은 2n: 50으로 동일하였으나 고성집단은 2n=50, In : 48인 염색체상이 한개체에서 동시에 발견되었고, 인형성부위의 위치 및 형태가 타 집단과 차이가 있었다. 전기영동을 실시하여 총 28개의 유전자를 검출, 분석하였다. 3종중 N. multifosciata 청도집단의 유전적 변이 (HDi 123, HG : .160)가 가장 높았고, N. toni 고성집단이 가장 적었다. ( HD= .017, HG= .015). N. yoni 고성집단과 강릉집단은 각 집단의 고유한 genetic marker를 다수 갖고 있으며 타집단들과 유전적 근연관계도 매우 먼 것으로 나타나 각기 독립된 별종으로 추정된다.

• The Korean Journal of Malacology
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• v.11 no.2
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• pp.171-179
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• 1995

한국산 다슬기류의 종별 유전적 변이를 알아보고 계통분류학적 근거를 제공하기 위하여 다슬기속(Simisulcospira)에 속하는 다슬기(S. libertina),주름다슬기,(S. forticosta), 곳체다슬기(S. gottschei), 좀주름다슬기(S. tegulata)및 참다슬기(S. coreana)등 5종을 전국 각지에서 채집하여 전기영동(starch gelelectrophoresis)를 이용해 분자유전학적 분석을 실시한 결과를 용약하면 아래와 같다. 유전적 다형형의 빈도는 58%에서 83%로 대단히 높았고 이형접합자의 평균 빈도도 50%내외였다. Rogers' D를 근거로 한 각 종간 유연관계는 주름다슬기와 곳체다슬기가 근연관계를 이루고 있었으며 이들 두 군과 다슬기는 유전적으로 거리를 두고 있었다. 이러한 결과는 패각의 형채와 비교할 때 유사한 경향을 보였다.

• Korean journal of applied entomology
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• v.17 no.4 s.37
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• pp.193-199
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• 1978

Five parents and their 10 $F_1$ crosses of maize (Zea mays L.) were tested by means of diallel analysis for the inheritance of peroxidase activity following Puccinia sorghi rust infection. Peroxidase activity was measured at day zero and at 2 days and 8 days after inoculation. Peroxidase activity was increased significantly by P. sorghi infection in all 15 genotypes after 8 days, but not after 2 days. Highly significant differences in peroxidase activity were detected among the 15 genotypes. The highly genera] resistant inbred, CM105, and its hybrids showed exceptionally high peroxidase activity in both healthy and infected plants. However, another highly resistant inbred, Oh545, showed exceptionally low peroxidase activity. Significant general combining ability (GCA) and specific combining ability (SCA) means quares were detected for peroxidase activity independent of disease. GCA mean spuares, however, were consistently a major contiribution to the inheritance of peroxidase activity in the infected plants whereas SCA men square contributions were minor. Rust resistant maize plants controlling mongenic dominant $Rp_1^d$gene showed stronger peroxidase reroxidase responses than their susceptible counterparts in the gel electrophoresis and densiometric tracings. The increased peroxidase activity occurred in both major leaf peroxidases, $Px_3\;and\; Px_7$.

• Journal of Practical Agriculture & Fisheries Research
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• v.4 no.1
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• pp.45-54
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• 2002

A lot of clones of the genus Dioscorea species have been introduced from some tropical and subtropical regions since 1997. The aim of this study was to determine characteristics of the production process in yams(Dioscorea spp). By utilizing 51clones of water yams(Dioscorea spp), some morphological characteristics were investigated at the field. Intraspecific genetic relationship of 51 variation types of the Yam classified by their external morphological characteristics such as leaf and tuber shape were assessed by DNA using random and specific primers. D. Alata and D. opposita were showed different relationship between yield and the growth of their aboveground parts. This suggests that even in the same species there were differences in yield volume and translocation of assimilation products, depending on the types. D. alata were distinguished from others Dioscorea species at 62% level in AFLP analysis. Also in principal component analysis, D. alata were showed the class from II to V.

• Journal of Life Science
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• v.18 no.6
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• pp.753-758
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• 2008

Genus Hemerocallis is a herbaceous species and some species among their taxa are very important herbal medicines. We evaluated representative samples of the eight taxa in Korea with inter simple sequence repeats (ISSR) markers to estimate phylogenetic relationships within taxa of this genus. The studied taxa were Hemerocallis fulva L., H. fulva for. kwanso, H. dumortieri Morren, H. coreana Nakai, H. hongdoensis M.G.Chung & S.S.Kang, H. middendorffi Trautv. et Mayer, H. thunbergii Baker, H. minor Miller. In addition, we investigated the genetic variation and structure of Korean populations of these taxa. The mean genetic diversity was 0.098 across species, varying from 0.068 to 0.123. A low level of genetic variation was found in populations of Hemerocallis species. Specially, gene diversity for H. minor was maintained the highest among genus Hemerocallis. An indirect estimate of the number of migrants per generation (Nm=0.218) indicated that gene flow was not extensive among Korean populations of Hemerocallis species. The phylogenic tree showed distinct three clades. One includes H. fulva, H. fulva for. kwanso and H. middendorffi. Another includes three Hemerocallis species, H. dumortieri, H. thunbergii and H. minor. The H. coreana and H. hongdoensis were shown as the sister group to the second clades. Although the size of sampling was not large enough for eight Korean Hemerocallis species, the analyses of ISSRs will certainly provide an enhanced view on the phylogeny of species.