Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Molecular Authentication and Genetic Polymorphism of Korean Ginseng (Panax ginseng C. A. Meyer) by Inter-Simple Sequence Repeats (ISSRs) Markers

ISSRs 마크에 의한 고려 인삼의 분자적 인증과 유전적 다형현상

  • Bang, Kyong-Hwan (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Lee, Sung-Woo (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Hyun, Dong-Yun (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Cho, Joon-Hyeong (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Cha, Seon-Woo (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Seong, Nak-Sul (Division of Ginseng & Medicinal Crops, National Institute of Crop Science, RDA) ;
  • Huh, Man-Kyu (Department of Molecular Biology, Dongeui University)
  • Published : 2004.06.01
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Abstract

Molecular authentication and genetic polymorphism of Korean ginseng cultivars and accessions were investigated using ISSR (inter-simple sequence repeat amplification) markers. Five primers among 56 produced clear and reproducible DNA fragments among seven cultivars and accessions. A total of 43 bands ranging from 250 bp to 1,700 bp from five primers were scored. Average number of bands per primer was 8.6 and only nine bands were polymorphic across the six Panax ginseng from Korea. Especially Chunpoong cultivar exhibited the highest level of polymorphism, whereas other accessions did not showed almost any polymorphism. Consequently, these ISSR markers will be available to differentiate Chunpoong cultivar from other major Korean ginseng cultivars and accessions, such as Yunpoong, Hwangsukjong and Jakyungjong, at the DNA level.

ISSR마크를 사용하여 고려 인삼의 품종 및 계통간 분자적 인증과 유전적 다형현상을 조사하였다. 56개의 ISSR 프라이머 중 5개가 일곱 품종 및 계통간 명확하고 재현성이 높은 DNA분절을 나타내는 최적 프라이머로 선택되었다. 전체 43밴드는 250 bp - 1,700 bp의 분자량을 가지며 프라이머당 8.6개의 밴드를 나타내었다. 고려 인삼에서 다형현상 정도는 20.9%였다. 특히 천풍 품종이 가장 높은 다형현상을 나타낸 반면 다른 품종은 거의 다형현상을 나타내지 않았다. 결론적으로 DNA수준에서 ISSR마크로 천풍이 다른 고려 인삼의 품종 및 계통인 연풍, 황숙종, 자경종과 구분에 이용될 수 있음이 판명되었다.

Keywords

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