• The Science & Technology
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• no.3 s.406
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• pp.6-7
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• 2003

껐다켰다 ··· 유전자 조작 기술 개발/ 음주로 인한 간질환 급증/ '산소화효소' 작용기전 세계 최초 규명/ 스팸메일 적발 시스템 가동된다/ 과학문화재단, '빅 프로젝트' 추진/ 범인 잡는 '뇌지문 탐지기'/ 우리나라 인터넷 이용률 세계 3위/ 한양대 이공계, 철학 모르면 졸업 못해

• Analytical Science and Technology
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• v.27 no.3
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• pp.147-152
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• 2014

RUVIS(Reflective Ultraviolet Imaging System) is an effective equipment that detects the location of latent fingerprint at crime scene using short wavelength ultraviolet of 254 nm. In this study, the degree of DNA damage in biological samples was compared depending on the distance and time of processing using four commonly used RUVIS. 50% of DNA was damaged by treating 10 seconds at 10 cm distance in 3 types of RUVIS such as Police RUVIS, SIRCHIE mini light and SIRCHIE RUVIS. In addition, the degree of DNA damage was increased as the distance was closer and the treatment time was longer. It showed that short wavelength UV could cause DNA damage when used close to the samples at crime scene. Therefore, it was suggested to use RUVIS at a distance of at least 1 m. The degree of DNA damage was not significant by Polilight which used long wavelength ultraviolet of 350 nm. As a result, the choice and usage of which UV light and RUVIS were critical for detection of fingerprint and successful DNA typing.

• Journal of Life Science
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• v.15 no.3 s.70
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• pp.427-433
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• 2005

With the completely discovery of the Drosophila genome sequence, the next great challenge is to extract its biological information by systematic expression and to perform functional analysis of the gene. Here we reported a proteome analysis of D. melanogaster with two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). The cell extracts of D. melanogaster, $200{\mu}g$ were resolved to more than 400 silver-stained spots by 2-DE. The most abundant protein spots were ranged from 4.0-7.5 of pI and from 15-90 kDa of molecular weight. The excised spots were destained and in-gel digested by trypsin. The masses of the resulting peptide mixtures were measured by MALDI-TOF-MS. Identified proteins were compared with measured peptide mass and a dynamic peptide searching database which is accessible via the internet. The results revealed that identified proteins were produced by 59 genes derived from 65 protein spots.

• Environmental Analysis Health and Toxicology
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• v.23 no.1
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• pp.1-9
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• 2008

유산균인 비피도박테리아는 사람과 동물에서 유익한 프로바이오틱 미생물로 알려져 있다. 본 연구에서는 이러한 비피도박테리아 균주의 분류를 위한 repetitive DNA element PCR fingerprinting (ERIC-또는 TAP-PCR)의 사용을 평가하였다. 사람분변으로부터 분리한 알려지지 않은 비피도박테리움 균주와 한국생명공학연구원 생물자원센터로부터 분양받은 표준균주를 가지고 분류 및 동정에 ERIC-PCR과 TAP-PCR을 이용한 RAPD-fingerprinting을 수행하였다. 그 결과 비피도박테리움 균주에 대한 속과 종단위의 분류가 가능하였으며, 실험에 사용된 모든 비피도박테리움 균주는 RAPD-fingerprinting 분석을 통해 유전적 다양성을 확인하였다. 또한 ERIC2와 TAP1 프라이머를 이용한 실험에서는 Bifidobacterium adolescenits 특이 유전자 단편을 확인하였으며 이는 B. adolescenits 균주의 동정에 유용할 것으로 사료된다.

• Journal of Periodontal and Implant Science
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• v.25 no.1
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• pp.153-166
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• 1995

54 clinical isolates of Actinobacillus actinomycetemcomitans showed distinct hybrdization patern(DAN fingerprinting patterns) when the bacterial DNA were hybridized with randomly cloned 4.7 - kb DNA probe. The frequency of the genotypic distribution demonstrated that type C was the most prevalent genotype, the next being D, NT, A, B, and E in the descending order. The most prevalent serotype was serotype c, the next being a, nd, and b in the descending order. It was noted that the one serotype can represent more than two different genotypes and that multiple genotypic variants can also exist in the periodontal pockets within the sam subject.

• Journal of Korean Society on Water Environment
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• v.24 no.2
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• pp.195-200
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• 2008

To analyze the riverine microbial community structure, genetic fingerprints and ecological indexes such as species abundances, diversity, evenness, dominance of targeted rivers in Gyeonggi Province were acquired and evaluated using terminal restriction fragment length polymorphism (T-RFLP) technique. Genetic fingerprinting technique such as T-RFLP, which is able to show the microbial community clearly unlike traditional culture-dependent techniques, was thought to be useful to analyse the riverine microbial ecosystem under various factors. Riverine ecosystem evaluation using visible organisms would give biased results with time, targeted organism and researcher. But, T-RFLP, which can exclude the subjected biases such as culture condition and identification, would be an option to understand natural ecosystem by including the microorganisms that defy culture but perform important functions.

• Journal of Oral Medicine and Pain
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• v.20 no.2
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• pp.515-528
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• 1995

The human genomic deoxyribonucleic acid(DNA) was extracted from the pulp, dentin of 22 teeth by clelex, phenol methods. Samples of the tooth-derived DNA were amplified by polymerase chain reaction(PCR), electrophosed for sex determination by detection of X-Y homologus amelogenin gene and D1S80 locus detection The following results have been achieved. 1. Chelex and phenol method are effective to sex determination in the pulp and dentin 2. Chelex method is not suitable for detection of D1S80 locus. 3. Concentration and purity of DNA for teeth using chelex method is lower than using phenol method. From the above investigation, chelex method is simple, rapid for sex determination, but it is not suitable for detection of VNTRs.

• Proceedings of the Korea Institutes of Information Security and Cryptology Conference
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• 2003.07a
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• pp.110-112
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• 2003

사람은 각각의 개인마다 전적으로 구분되는 생물학적인 고유한 특징을 가지고 있다. 유전자 형질, 지문, 목소리, 얼굴의 생김새, 눈동자의 홍채 등이 그러한 특징들이며, 이러한 개개인의 생물학적 고유성을 이용하여 자동으로 그 사람의 신원을 인식하는 시스템은 높은 신뢰도와 편리성 때문에 많은 연구가 되어왔고 현재 다양한 곳에서 실제 적용을 하여 사용되고 있다. 본 논문에서는 생체인식 시스템 중에서도 높은 신뢰도를 자랑하고 있는 홍채인식과 웨이블릿 변환을 이용한 홍채 코드 생성과정을 다루어 보았다. 화상처리 알고리즘과 최근 신호처리의 분야에서 많이 사용되고 있는 웨이블릿 변환을 중점으로 서술했으며 웨이블릿 변환을 응용한 여러 가지 인식 알고리즘의 적용을 개발 목표로 삼았다. 실제 홍채인식 시스템은 높은 보안을 필요로 하는 곳에 적용되어 상용화가 되어있고 많은 자료들이 공개가 되어 이미지 처리를 공부하는 사람들에게 좋은 아이템이라 생각한다.

• Proceedings of the Korea Information Processing Society Conference
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• 2013.05a
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• pp.268-271
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• 2013

CCTV는 사람들 생활에서 없어서는 안 되는 유용한 존재로 자리매김 했다. 가장 큰 장점은 범죄 수사과정에서 유전자나 지문감식에 견줄 만큼 기여도가 크다는 점이다. 폭력 및 범죄를 해결하는데 유용하게 쓰이고 있고 날로 발전하고 있는 상황이다. 본 연구에서는 최근 공간정보기술의 발달에 따라 여러 분야에 공간 정보 기반의 관제시스템이 운영 중이거나 추진 중에 있으며, 지능형 영상감시를 위한 동향과 CCTV 설치에 따른 합법성과 프라이버시 보호에 대해 연구를 한다.

• The Korean Journal of Mycology
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• v.31 no.2
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• pp.103-113
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• 2003

Twenty five isolates of Alternaria were obtained from various solanaceous crops in Korea. For all isolates, morphological characteristics of the conidia were determined and compared with those of representative isolates of A. solani and A. tomatophila. A selection of the isolates and the representative Alternaria isolates were evaluated for Pathogenicity to potato, tomato, egg plant and red pepper. Molecular characteristics of 17 isolates of Alternaria inculding the representative isolates were determined using sequence analysis of IRS rDNA and histone H3 gene, and URP-PCR analysis. Based on morphological characteristics, the isolates from the solanaceous crops were grouped as identical or very similar to either A. tomatophila (ATO), A. solani (ASO), and unidentified Alternaria sp. (ASP). Isolates of ASO were moderately pathogenic to all the solanaceous crops tested, but ATO isolates were highly pathogenic to tomato and the ASP isolate was pathogenic only to potato. Among the molecular markers used in this study, the URP-PCR analysis was found to be appropriate for taxonomic resolution of these species. Based on the conidial morphology, pathogenicity test and molecular characteristics, A. tomatophila (early blight of tomato) could be distinguished from A. solani (early blight of potato), and the Alternaria sp. (ASP) from potato, which was closely related to ASO in conidial morphology, was considered as a new species.