• Title/Summary/Keyword: 유전자클로닝

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Glyphosate 저항성 pseudomonas sp. strain HG-1 의 분리 및 저항성 유전자의 클로닝

  • 이병철;조홍범;채영규;최영길
    • Korean Journal of Microbiology
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    • v.30 no.1
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    • pp.42-46
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    • 1992

  • From the soil environment which is treated with herbicides. we isolated strain having high resistance against glyphosate. After being identified. the isolated strain was turned out to be Pseudomc~nu.c~c ~paciua nd to have intense tolerance lo the 10 mM glyphosate. The isolated strain shows slow, growth rate about twenty hours in glyphosate comparing with that in inorganic phosphate. As a result of confirming the p~~sitioonf glyphosate resistant gene. it was proved to exist in chromosome. After cloning it into E coli C600, transformants E. coli THC-101 and plasmid pGR19 were obtained.

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