• Journal of Sericultural and Entomological Science
• /
• v.36 no.2
• /
• pp.115-118
• /
• 1994

Differences of feeding response of 280 silkworm genetic stocks and 71 breeding lines to MP-O artificial diet and the mode of the inheritance were investigated. Feeding response to MP-O diet varied markedly between the silkworm varieties, and the non-normally distributed curve for the response was observed. From the genetic analysis, the high feeding response to MP-O diet was recessive to the low feeding response. Therefore it is considered that the high feeding response of the newly hatched silkwrom larvae to MP-O diet is controlled by a recessive gene.

• Korean Journal of Breeding Science
• /
• v.40 no.2
• /
• pp.159-163
• /
• 2008

A New spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Water Fog' was bred by open pollination of 'Angaesoguk'. Its characteristics were investigated three times from 2004 to 2006 under forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Water Fog' was 24 October, but year-round production was possible by shading and lighting. It had flower diameter of 2.3 cm, 46.5 petals and 34.4 flowers per stem in autumn. The petal was narrow(0.4cm) and the color of petal was white with presence of green at flower center. Days to flower from short-day start for 'Water Fog' was about 48 in spring. 'Water Fog' showed the vase life of 22.6 days in autumn. This cultivar was registered for a commercialization in 2007. 'Water Fog' would be cultured under greenhouse and rain shelter condition in Korea.

• Horticultural Science & Technology
• /
• v.31 no.6
• /
• pp.764-771
• /
• 2013

Recently, Cucumber mosaic virus (CMV)-P1 infection in pepper cultivation is very serious problem, which causes low marketability and yield. In this research, 56 domestic pepper cultivars including 20 PR (Phytophthora resistance) cultivars, 31 foreign pepper lines collected from USA and 112 genetic pepper resources form RDA Genebank were used for CMV-P1 resistance evaluation. Resistance evaluation was performed at 24 days and 51 days after artificial inoculation of peppers by enzyme-linked immunosorbent assay (ELISA). Among the 36 domestic cultivars 'Muhanjilju' were resistant to CMV-P1. All 20 PR cultivars and 29 foreign peppers except 'NuMex Twilight' and 'Chainese Giant' were susceptible. Among 112 pepper lines from RDA Genebank, nine pepper lines were resistant, and 17 pepper lines were moderately resistant, and 86 pepper lines were susceptible. Almost all domestic peppers on the market were highly susceptible to CMV-P1, whereas 17.2% of foreign pepper cultivars and genetic pepper lines from RDA Genebank were resistant or moderately resistant. Resistant pepper lines selected in this study can be used as genetic sources for breeding CMV-P1 resistant pepper.

• Korean Journal of Plant Resources
• /
• v.26 no.4
• /
• pp.491-502
• /
• 2013

Persimmon (Diospyros kaki Thunb) fruit is one of the most important fruit and have been cultivated from ancient times in Korea. In this study, we found 16 EST-SSR markers that contained one or more EST-SSR sites from 246 cDNA sequences. The developing of EST-SSR marker analysis from 42 persimmon cultivars was compared by genetic relationships and morphological relationships using 6 qualitative traits (fruit related 6 traits) and 19 quantitative traits (flower related 19 traits). In this study, 25 primer sets were tested to identify PCR polymorphism and 14 potential EST-SSR primer pairs were selected. The result of morphological relationship EST-SSR marker analysis showed that the coefficient 0.02 was difficult to categorize in several groups. And then, coefficient 0.77 of genetic relationship showed that the group was classified as four groups. The result of correlation distance between genetic relationship and morphological relationship were investigated was low significance (-0.03). Our results also provided an optimized method for improvement of breeding efficiency and introduce of superior character at persimmon cultivars using EST-SSR markers which was useful for further investigation.

• Journal of Plant Biotechnology
• /
• v.49 no.3
• /
• pp.178-186
• /
• 2022

The tetraploid Solanum acaule is a wild potato species from Bolivia widely used for potato breeding because of its diverse attractive traits, including resistance to frost, late blight, potato virus X, potato virus Y, potato leafroll virus, potato spindle tuber viroid, and cyst nematode. However, the introgression of useful traits into cultivated potatoes via crossing has been limited by differences in endosperm balance number between species. Somatic fusion could be used to overcome sexual reproduction barriers and the development of molecular markers is essential to select proper fusion products. The chloroplast genome of S. acaule was sequenced using next-generation sequencing technology and specific markers for S. acaule were developed by comparing the obtained sequence with those of seven other Solanum species. The total length of the chloroplast genome is 155,570 bp, and 158 genes were annotated. Structure and gene content were very similar to other Solanum species and maximum likelihood phylogenetic analysis with 12 other species belonging to the Solanaceae family revealed that S. acaule is very closely related to other Solanum species. Sequence alignment with the chloroplast genome of seven other Solanum species revealed four InDels and 79 SNPs specific to S. acaule. Based on these InDel and SNP regions, one SCAR marker and one CAPS marker were developed to discriminate S. acaule from other Solanum species. These results will aid in exploring evolutionary aspects of Solanum species and accelerating potato breeding using S. acaule.

• Journal of Life Science
• /
• v.33 no.8
• /
• pp.632-638
• /
• 2023

A soybean cultivar with a green seed coat and cotyledon contains high levels of lutein, which is beneficial for eye health. Plus, antinutritional components such as lipoxygenase, Kunitz trypsin inhibitor (KTI), lectin and stachyose exist in the mature seed. The genetic elimination of these antinutritional factors is a necessary step in green soybean breeding. This research was conducted to improve a new green soybean line with the green cotyledon and tetra null genotype (lox1lox2lox3tilers2) in terms of lipoxygenase, KTI, lectin and stachyose. We used five germplasms to develop a breeding population. A total of 69 F₂ seeds were obtained from the cross of parent 1 and parent 2, and from those, 21 F₂ seeds were selected that had the green seed coat color, and which were free of lectin protein. Next, four F₂ plants with the green seed coat and tetra null genotype were selected from the breeding population derived from four genotypes. The absence of lipoxygenase, KTI and lectin proteins was confirmed in the F₅ strain. The breeding line has a green seed coat, green cotyledon and white hilum color. The 100-seed weight and stachyose content for the breeding line were 30.7 g and 2.40 g/kg, respectively. The line selected in this study could be used as a cultivar or parent to improve colored soybean cultivars through the removal of antinutritional components such as lip- oxygenase, KTI, lectin and stachyose.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.15
• /
• pp.33-59
• /
• 1974

A series of experiments were conducted at Crop Experiment Station from 1968 to 1971 to obtain basic information on effects of selection on yield of wheat varieties. Heritability estimates, correlation and path coefficients computed for yield and yield related characters from fixed variety groups-Korean, U.S., and Japan; early and late-$F_2$ and $F_3$ generations were studied. The same estimates for fixed variety groups grown under different fertilizer levels and years were also studied. The results were summarized as follow: 1. Three variety groups classified by their origins as Korea, United States and Japan showed high heritability estimates for heading date, plant height and spike length. The heritability estimates for grain number per spike and 1, 000 grain weight were moderate and those for the number of spikes per plant and grain yield were low. Very low estimates of heritability were obtained for grain number per spike and yield of variety group from the Unites States. 2. High genotypic correlation coefficients between 1, 000 grain weight and yield were obtained for all variety groups originated from Korea, United States and Japan and early variety group, except late variety group. The genotypic correlation coefficients between grain number per spike and yield were also high for all variety groups except variety group originated from the United States. 3. The direct effects of 1, 000 grain weight in terms of path-coefficients to yield were high for all variety groups except late variety group. 4. High genotypic correlation coefficients were obtained between 1, 000 grain weight and yield in $F_2$ from. two crosses. The same degree of genotypic correlation coefficients were obtained between grain number per spike and yield, although slight differences in its magnitude were found depending upon the cross combination. 5. The analysis of path-coefficients in $F_2$ shows that the direct effects of yield components to yield were negligible. 6. The characters that showed high genotypic correlation coefficients with yield in $F_3$ were 1, 000 gnain weight and grain number per spike. These characters showed also high direct effects to yield. 7. No great variations of heritability estimates for heading date, plant height and spike length were obtained for either fertilizer responsive or non responsive variety group due to fertilizer levels applied. 8. Heritability estimates of 1, 000 grain weight in fertilizer responsive group and yield in fertilizer nonresponsive group were high as level of fertilizer increased. 9. Heritability estimates for grain number per spike and 1, 000 grain weight of fertilizer non-responsive-group were higher than those of fertilizer. responsive group. 10. Genotypic corretation coefficients between yield and 1, 000 grain weight in fertilizer responsive group were getting lower as the level of fertilizer increased and those in fertilizer non-responsive group were vice versa. 11. Genotypic correlation coefficient between yield and spike number per plant in fertilizer responsive group was high. However, the genotypic correlation coefficient between yield and spike number per plant in fertilizer non-responsive group was low. 12. The direct effects of 1, 000 grain weight to yield were higher than other yield components either in fertilizer responsive or non-responsive group regardless of levels of fertilizer applied. The spike number per plant, however, was high only when high level of fertilizers were applied to fitilizer responsive group. 13. Slight variations of heritability estimates for heading dates, plant height, spike length, grain number per spike and 1, 000 grain weight were obtained between years. However, the spike number per plant with low heritabilility showed great variation between years. 14. The character that showed high genotypic correlation coefficients with yield in two years was 1, 000 grain weight, and this character was also high in direct effect to yield in terms of path-coefficients. 15. From the above experimental results, it might be concluded that 1, 000 grain weight would be one of the most important characters to increase the effects of selection for yield in wheat breeding in Korea.

• Korean Journal of Breeding Science
• /
• v.40 no.4
• /
• pp.377-386
• /
• 2008

The growth performance of Eastern white pine (Pinus storbus L.) was studied with six provenances in four plantations. All growth performances were significantly different among provenances and plantations. The most outstanding source in volume growth at age 39 is North Carolina in all plantations and this trends had been started from four years old seedlings. In plantations, the growth is best in ChunCheon plantation while that is worst in GunPo plantation. The Effect of plantations was thought to be larger than that of provenances growth of P. strobus. A pattern of growth by ages was different by among plantations and even ate age about 40, the growth was still vigorous. Annual precipitation, foggy days, altitude and sand contents in soil are positively correlated with growth and the correlations between by ages were very high.

• The Journal of the Korean bone and joint tumor society
• /
• v.6 no.4
• /
• pp.143-151
• /
• 2000

Purpose : The p53 tumor suppressor gene is one of the most frequently altered genes in human malignancies. We try to explore the implication of p53 alteration in Ewing's sarcoma. Materials and Methods : We analyzed 35 paraffin blocks to explore the deletion and sequence alterations of p53. Results : Quantitative PCR analysis showed that 2 tumors showed a homozygous deletion of the gene. Mutational analysis of exons 4 to 9 of p53 by PCR-SSCP revealed that 3 tumors carry sequence alterations in exons 5 or 8, and DNA sequencing analysis identified missense point mutations. Conclusion : Taken together, our data demonstrate that p53 is genetically altered in a small fraction of Ewing's sarcoma.

• Journal of Pharmacopuncture
• /
• v.7 no.2
• /
• pp.19-28
• /
• 2004

봉독은 관절염 치료를 비롯한 여러 질환에 그 응용범위가 넓어지고 있으며 기전규명과 새로운 치료효과 개발을 위한 연구가 필요하다. 연골의 파괴는 진행된 각종 관절병증의 공통 병리기전이며 연골세포의 기능이상은 이 기전에 중요한 의미를 지닌다. 사람 연골세포의 특성을 유지하고 있는 HTB-94 연골육종세포를 배양하고 봉독을 처치했을 때의 유전자 발현양상을 microarray를 이용하여 관찰하였다. 대조군에 비해 4배 이상 발현의 차이가 있는 경우를 유의한 것으로 보았을 때 microarray의 344개 유전자중 봉독처치시 발현이 증강되는 유전자는 없었으며 발현이 억제되는 유전자는 interleukin 6 receptor, interleukin 1 alpha, tissue inhibitor of metalloproteinase 1, matrix metalloproteinase 1, tumor necrosis factor (ligand) superfamily, members 4, 8 and 12, and caspases 2, 6, and 10등 35개가 관찰되었다. Microarray를 통한 유전자발현 분석을 통해 관절염에 대한 봉독치료의 기전을 시사하는 유용한 자료를 얻을 수 있었으며 앞으로 보다 넓은 범위에 대한 연구가 필요할 것이다.