• Title/Summary/Keyword: 유전다양성

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Effect of Antioxidant Activity and Induction of DNA Damage on Human Gastric Cancer Cell by Rubus coreanus Miquel (복분자 추출물의 항산화활성 및 인간 위암 세포주에 대한 유전적 손상 유도)

  • Jeon, Sang-Kyung;Lee, Ji-Won;Lee, In-Seon
    • Journal of Life Science
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    • v.17 no.12
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    • pp.1723-1728
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    • 2007
  • Rubus coreanus Miquel (RCM), a type of red raspberry, grows wild in Korea and China and its unripe fruit is used as a folk medicine for the treatment of impotence and as a diuretic. RCM was extracted with methanol and then further fractionated it into for different types. In this study, we investigated the antioxidant activity of a RCM extract (ext.) and its fraction (fr.). DPPH free radical scavenging activity assay, total polyphenols contents, total flavonoids contents assay were used to analyze antioxidant activity. The DPPH free radical scavenging activity $(RC_{50}:1.67{\mu}g/ml)$ and total polyphenols contents $(546.25{\mu}g/mg)$ were higher in butanol fraction than in other fr. And total flavonoids contents was higher in ethylacetate fr. $(141.78{\mu}g/mg)$. We applied comet assay to measure the DNA damage in the individual cells and exposed time course at $IC_{50}$. Comet assay is a rapid and sensitive fluorescent microscopic method to examine DNA damage and repair at individual cell level. The butanol fro from RCM significantly induced 54.12%, 57.95% of DNA damage after treated RCM for 8 hr. In conclus

Clonal Variation of Flowering in Pinus thunbergii Seed Orchard (해송 채종원(採種園)에서 개화특성(開花特性)의 클론 변이(變異))

  • Han, Sang-Urk;Choi, Wan-Yong;Chang, Kyong-Hwan;Kim, Tae-Su;Song, Jeong-Ho
    • Journal of Korean Society of Forest Science
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    • v.90 no.6
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    • pp.717-724
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    • 2001
  • This study was conducted in an attempt to quantify some traits that may be involved in the reproductive process of Pinus thunbergii. During the past 6 years (1995-2000), we surveyed flowering patterns of 60 P. thunbergii clones in a seed orchard which was established in Anmyon-do, 1979. From this survey, we estimated clonal contributions of male and female flowers and sexual asymmetry. Among 60 clones, 18 clones (30%) accounted for 0.37 (1999)~0.46 (1995) of clonal contribution in female flowers and 0.44 (1999)~0.57 (1995) of clonal contribution in male flowers. As compared with the previous reports in other pine species, more clones made contributions to reproductive process in P. thunbergii seed orchard. The relative effective population numbers for females (v♀) varied from 0.59 (1995) to 0.91 (1999) and those for males ( v♂) were between 0.56 (1995) and 0.83 (1998) at gamete level. This showed that the female gametes effectively contribute to the reproductive process more than did the male gametes. The relative effective population numbers at clonal level ($v_b$) were in the range of 0.72 (1995) and 0.93 (1999). More than 73% of the total clones showed values of 0.4~0.6 ($0.5{\pm}0.1$) in maleness index. The values averaged over all the clones were temporally variable with the range of 73% in 1996 and 100% in 2000. The degree of sexual asymmetry ($A_s$) ranged from 0.09 to 0.26. These values were relatively low when compared with those of other trees, suggesting that P. thunbergii seed orchard remains a high level of homogeneity in the number of male and female. All of the various types of effective population numbers were positively correlated with each other while they were negatively correlated with the degree of sexual asymmetry.

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Characteristics of mulberry cultivar 'Sangmaru' (Morus alba L.) for mulberry fruit production (오디 생산용 뽕나무 품종 '상마루'의 특성)

  • Sung, Gyoo Byung;Kim, Yong Soon;Kim, Kee Young;Ji, Sang Duk;Kim, Hyun-bok
    • Journal of Sericultural and Entomological Science
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    • v.53 no.2
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    • pp.110-117
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    • 2015
  • We bred a mulberry cultivar named Sangmaru, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at three places (Suwon, Cheongju, and Jangseong) for seven years from 2008. This is tetraploidy variety belonging to Morus alba L. made by colchicine treatment on growing point of 'Chungil (Morus alba L.)' winter buds. Sangmaru was high yielding cultivar in fruit productivity by 6% compared to control cultivar 'Chungil (Morus alba L.)' for five years. Although Sangmaru was similar in sugar content of mulberry fruits, it was bigger than that of 'Chungil'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

Analysis of Twinkling Artifacts Caused by Kidney Stones on Abdominal Ultrasound (복부 초음파 검사에서 신장결석으로 인해 발생되는 Twinkling 인공물에 관한 분석)

  • Kim, Ju-Hee;Jang, Hyon-Chol;Cho, Pyong-Kon
    • Journal of the Korean Society of Radiology
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    • v.15 no.5
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    • pp.637-642
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    • 2021
  • Kidney stones are largely classified into kidney stones, ureter stones, and urolithiasis depending on the location of their occurrence. Therefore, in this study, from January 2019 to June 2021, kidney stones found in 112 patients with flank pain or who visited for abdominal ultrasonography at a general hospital located in Daegu were diagnosed with urolithiasis. We wanted to investigate the effect on twinkling artifacts. As a result of the study, the incidence of twinkling artifacts due to kidney stones was relatively high in the longitudinal scan among the scan methods. As the number of kidney stones increased, the incidence of twinkling artifacts increased by 1.296 times (p<0.05). As the kidney stone size increased, the incidence of twinkling artifacts increased by 0.086-fold (p<0.05). It was found that the number and size of kidney stones are factors affecting twinkling artifacts. Since the effect of kidney stones on twinkling artifacts is related to the number and size of kidney stones, continuous attention should be paid to helping the detection of kidney stones by using variables affecting twinkling artifacts.

Calibration of cultivar parameters for cv. Shindongjin for a rice growth model using the observation data in a low quality (저품질 관측자료를 사용한 벼 생육 모델의 신동진 품종모수 추정)

  • Hyun, Shinwoo;Kim, Kwang Soo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.1
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    • pp.42-54
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    • 2019
  • Crop models depend on a large number of input parameters including the cultivar parameters that represent the genetic characteristics of a given cultivar. The cultivar parameters have been estimated using high quality data for crop growth, which require considerable costs and efforts. The objective of this study was to examine the feasibility of using low quality data for the parameter estimation. In the present study, the cultivar parameters for cv. Shindongjin were estimated using the data obtained from the report of new cultivars development and research from 2005 to 2016. The root mean square errors (RMSE) of the heading dates were less than 3 days when the parameters associated with phenology were estimated. In contrast, the coefficient of determination for yield tended to be less than 0.1. The large errors incurred by the fact that no growth data collected over a season was used for parameter estimation. This suggests that detailed observation data needs to be prepared for parameter calibration, which would be aided by remote sensing approaches. The occurrence of natural disasters during a growing season has to be considered because crop models cannot take into account the effects of those events. Still, our results provide a reasonable range for the parameters, which could be used to set the boundary of a given parameter for cultivars similar to cv. Shindongjin in further studies.

Development of Specific SNP Molecular Marker from Thistle in the DNA Sequences of Chloroplast TrnL-F and Matk Region Using HRM Analysis (엉겅퀴의 엽록체 TrnL-F와 Matk 영역 염기서열의 HRM 분석을 통한 특이적 SNP 분자마커의 개발)

  • Lee, Shin-Woo;Lee, Soo Jin;Kim, Yun-Hee
    • Journal of Life Science
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    • v.29 no.5
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    • pp.524-529
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    • 2019
  • Medicinal plants resources are becoming important assets since their usages have been expanded to the development of functional foods for human health, cosmetics and pharmaceutical industries. However, their phylogenetic origins and names are different from each country and quite often they are mixed each other resulting in the confusion for consumers. Particularly when they are very similar based on their morphological characteristics and distributed, it is extremely difficult to differentiate their origins even by specialists. Therefore, identification of each plant species is important for standardizing herbal medicine. Thistle is a medicinal and perennial plant. Obtaining information about the genetic diversity of plant populations is highly important for conservation and germplasm utilization. Although thistle is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish from other similar species from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from chloroplast genomic sequences to identify distinct Korean-specific thistle species via high resolution melting (HRM) curve analyses. We performed molecular authentication of four different kinds of thistle species from different regions using DNA sequences in the trnL-F and matK chloroplast intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific thistle species from different country.

SNP Markers Useful for the Selection of Yellow-fleshed Peach Cultivar (황육계 복숭아 품종 선발용 SNP 마커)

  • Kim, Se Hee;Kwon, Jung-hyun;Cho, Kang Hee;Shin, Il Sheob;Jun, Ji Hae;Cho, Sang-Yun
    • Korean Journal of Plant Resources
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    • v.34 no.5
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    • pp.443-450
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    • 2021
  • Peach flesh color is commercially important criteria for classification and has implications for nutritional quality. To breed new yellow-fleshed peach cultivar many cross seedlings and generations should be maintained. Therefore it is necessary to develop early selection molecular markers for screening cross seedlings and germplasm with economically important traits to increase breeding efficiency. For the comparison of transcription profiles in peach varieties with a different flesh color expression, two cDNA libraries were constructed. Differences in gene expression between yellow-fleshed peach cultivar, 'Changhowon Hwangdo' and white-fleshed peach cultivar, 'Mibaekdo' were analyzed by next-generation sequencing (NGS). Expressed sequence tag (EST) of clones from the two varieties was selected for nucleotide sequence determination and homology searches. Putative single nucleotide polymorphisms (SNPs) were screened from peach EST contigs by high resolution melting (HRM) analysis, SNP ID ppa002847m:cds and ppa002540m:cds displayed specific difference between 17 yellow-fleshed and 21 white-fleshed peach varieties. The SNP markers for distinguishing yellow and white fleshed peach varieties by HRM analysis offers the opportunity to use early selection. This SNP markers could be useful for marker assisted breeding and provide a good reference for relevant research on molecular mechanisms of color variation in peach varieties.

Analysis of Globalization After COVID-19 Based on Network (네트워크 기반 코로나바이러스감염증-19 이후 세계화 분석)

  • Ryu, Jea Woon;Kim, Hak Yong
    • The Journal of the Korea Contents Association
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    • v.21 no.6
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    • pp.62-70
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    • 2021
  • 2020 was a year in which the world spent in disorder due to the pandemic of Coronavirus infection-19(COVID-19). The pandemic was at the beginning of a turning point in history. For examples, the Black Death(Pest) that destroyed the feudal system of medieval Europe in the 14th century, smallpox that led to the destruction of the Inca Empire by Spain in the 17th century, and the Spanish flu that ended World War I early. The great transformation that will come after COVID-19 is presented from various fields and perspectives, but the understanding and direction of the transformation is ambiguous. This study attempts to derive and to analyze core terms based on a network of the future of globalization after COVID-19. Four Networks related to globalization, anti-globalization, and globalization and digitalization after COVID-19 were established respectively. A network integrating four networks was also constructed. The core terms were extracted from the hub nodes, the stress centrality, and the simplified network to which the K-core algorithm was applied. After COVID-19, the changes in globalization were analyzed from the extracted core terms. This study is thought to be meaningful to propose a method of deriving and analyzing core terms based on a network in understanding social changes after COVID-19.

Applicability Analysis on Estimation of Spectral Induced Polarization Parameters Based on Multi-objective Optimization (다중목적함수 최적화에 기초한 광대역 유도분극 변수 예측 적용성 분석)

  • Kim, Bitnarae;Jeong, Ju Yeon;Min, Baehyun;Nam, Myung Jin
    • Geophysics and Geophysical Exploration
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    • v.25 no.3
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    • pp.99-108
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    • 2022
  • Among induced polarization (IP) methods, spectral IP (SIP) uses alternating current as a transmission source to measure amplitudes and phase of complex electrical resistivity at each source frequency, which disperse with respect to source frequencies. The frequency dependence, which can be explained by a relaxation model such as Cole-Cole model or equivalent models, is analyzed to estimate SIP parameters from dispersion curves of complex resistivity employing multi-objective optimization (MOO). The estimation uses a generic algorithm to optimize two objective functions minimizing data misfits of amplitude and phase based on Cole-Cole model, which is most widely used to explain IP relaxation effects. The MOO-based estimation properly recovered Cole-Cole model parameters for synthetic examples but hardly fitted for the real laboratory measures ones, which have relatively smaller values of phases (less than about 10 mrad). Discrepancies between scales for data misfits of amplitude and phase, used as parameters of MOO method, and it is in necessity to employ other methods such as machine learning, which can deal with the discrepancies, to estimate SIP parameters from dispersion curves of complex resistivity.

Water Absorption and Alkali Digestibility by Seed Size in Local Black Soybean Collections (종실크기에 따른 수집 검정콩 수분흡수 및 알칼리 붕괴도)

  • Kim, Su-Kyeong;Kim, Dae-Ho;Son, Beom-Young;Kang, Dong-Ju;Han, Kyung-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.4
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    • pp.452-458
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    • 1997
  • Seed characteristics such as seed size, seed shape and cotyledon color are important in relation to processing and cooking of soybean. Seed shape, water absorption rate, and alkali digestibility were evaluated for 28 local black soybean collections. Water uptake rate was low in small seed size at low temperature(5, 2$0^{\circ}C$). However, higher water absorption rate was observed at high temperature(40, 6$0^{\circ}C$) regardless of seed size. When seeds soaked up water, it reached to water absorption equilibrium after 10 hrs below 2$0^{\circ}C$, within 10 hrs at 4$0^{\circ}C$, and around 4 hrs at 6$0^{\circ}C$, respectively. Significant positive correlations were observed between 100 seed weight and length, width and thickness of seed, respectively. Alkali digestibility value(ADV) was higher in large seed group than in small seed group. Among seed characteristics, significant positive correlation was shown between ADV and 100 seed weight, length, width, thickness and hilum length of seed, respectively. Soybean seed with green cotyledon showed higher ADV than that with yellow cotyledon.

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