• Journal of the Korean Arthroscopy Society
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• v.16 no.2
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• pp.134-139
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• 2012

Purpose: To report the treatment results of arthroscopic osteochondroplasty in osteochondral lesion of the talus. Materials and Methods: This study included 32 patients who had osteochondral lesion of the talus treated with arthroscopic osteochondroplasty and were able to be followed up over 3 years from 1999 to 2007. The mean follow up period was 48 (38-108) months, and 22 male patients and 10 female patients with a mean age of 34 (20-56) years were included. Locations of lesion were evaluated with simple X-ray and magnetic resonance imaging (MRI), and severities of lesions were classified by Berndt and Harty classification. All cases were treated by arthroscopic debridement or microfracture. The treatment results were assessed by American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot scale and subjective and functional score of Kailkkonen. The group treated by arthroscopic microfracture was compared with the group treated by arthroscopic debridement. Results: The mean AOFAS ankle-hindfoot scale was significantly improved 53 (${\pm}7$) points preoperatively to 84 (${\pm}8$) points postoperatively (P<0.001). The mean subjective and functional score was also improved 55 (${\pm}8$) points preoperatively to 88 (${\pm}8$) points postoperatively with statistical significant (P<0.001). There were no relations between the severity of lesions and the postoperative functional score of ankle (P>0.05). There were no significant differences in the clinical results between the group treated with arthroscopic microfracture and the group treated with arthroscopic debridement (P>0.05). Conclusion: Arthroscopic osteochondroplasty of osteochondral lesion of the talus shows excellent result in terms of patients' satisfaction. There were no significant differences in the clinical results between arthroscopic microfracture group and debridement group.

• Journal of Bio-Environment Control
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• v.14 no.3
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• pp.203-211
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• 2005

The experiment was conducted to investigate the nutrition absorption pattern in the growth stages and develope the optimal nutrient solution hydroponically grown the tomato in closed substrate culture system with the nutrient solution of National Horticultural Research Station in Japan into 1/2S, 1 S, and 2S. When plant was grown in 1/2 S, the growth and yield were high and the pH and EC in the rooting zone were stable. Suitable composition of nutrient solution for tomato was $NO_3-N$ 7.1, $PO_{4}$-P 2.1, K 4.0, Ca 3.1, Mg 1.2, and $SO_{4}-S\;1.2\;me{\cdot}L^{-1}$ in the early growth stage and $NO_3-N$ 6.5, $PO_4-P$ 2.3, K 3.4, Ca 3.1, Mg 1.1, and $SO_4-S\;1.1\;me{\cdot}L^{-1}$ in the late growth stage by calculating a rate of nutrient and water uptake. To estimate the suitability for the nutrient solution of tomato in a development of optimum nutrient solution of tomato developed by Wonkwang university in korea (WU), plant was grown in perlite substrate supplied with different solution and strengths(S) by research station for greenhouse vegetable and floricultuin in the Netherlands (Proefstation voor tuinbouw onder glas te Naaldwijk; PTG) of 1/2 S, 1 S and 2 S, respectively, The growth was good at the PTG and WU of 2 S in early growth stage, and at the WU 2S in late growth stage. The highest yield of tomato obtained in the WU of 2 S, although blossom-end rot was appeared in all treatments. pH and EC in root zone of WU of 2 S were stable during the early and late growth stage. Therefore when plant was grown in WU of 2 S, N and P content in the nutrient solution need to low, according N and P content of their leaves were high in WU of 2 S.

• Journal of Wetlands Research
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• v.22 no.2
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• pp.81-91
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• 2020

As part of method for ecologically utilizing abandoned paddy, potential of the abandoned paddy as a target site for ex-situ conservation of 9 endangered species of different life form was confirmed by considering the nature of rice paddy. In order to create Dum-bung, a component of traditional Korean rice paddy, a part of the abandoned paddy was modified to change the water environment. The seeds, asexual reproductive and sexually reproductive individuals of each endangerd species were transplanted into the abandoned paddy to observe the survival rate and phenological response for 1 year, and then monitored for 2 years. As a result, Hydrophyte 4 taxa (Euryale ferox, Saururus chinensis, Dysophylla yatabeana, Menyanthes trifoliata), Geophyte taxa 1 (Epilobium hirsutum), and Hemicryptophyte taxa 1 (Cicuta virosa) could be introduced into the abandoned paddy. In particular, Euryale ferox, Dysophylla yatabeana, and Menyanthes trifoliata should be introduced into Dum-bung, and Saururus chinensis, Epilobium hirsutum, and Cicuta virosa should be introduced into paddy wetland. Growth of Euryale ferox and Brasenia schreberi was inhibited by herbaceous species, and the growth of Epilobium hirsutum was inhibited by herbivores. Therefore, in order to help efficient settlement of endangered plants introduced in abandoned paddy, it is necessary to remove herbs that inhibit growth and to manage herbivores. In addition, it is necessary to prevent the collapse of paddy bank by planting on the paddy field trees or herbaceous forming vegetation mat. When using abandoned paddy ecologically, it is effective to diversify the moisture environment by creating a Dum-bung to increase biodiversity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.10
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• pp.1510-1516
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• 2015

The objective of this study was to investigate the quality and sensory characteristics of sweet persimmon mashes and spirits fermented by three different yeasts [Saccharomyces species, labeled as LB (Lalvin BM $4{\times}4$), LD (Lalvin DV10), and UC (Uvaferm CM)]. In the production of sweet persimmon wines, UC had the highest alcohol content of 10.20% (v/v) after 9 days of fermentation (P<0.05). The range of pH after fermentation was 3.63~3.75. The total aerobic bacteria and yeast contents increased until day 3 and then continuously decreased to approximately 8.60 log CFU/mL and 8.20 log CFU/mL, respectively. Reducing sugar contents were 3.37% in UC, 3.91% in LD, and 4.05% in LB after fermentation. Total sugar contents were 4.89% in UC, 6.24% in LD, and 5.47% in LB after fermentation. Two-stage single-pot distillation was conducted to produce sweet persimmon spirits. While conducting second distillation, spirits were collected every 100 mL fraction. The alcohol contents gradually decreased as more fractions were collected. The amounts of acetaldehyde were 226 mg/L for LD, 225 mg/L for LB, and 310 mg/L for UC in the first fraction. LD produced the highest volume (677.8 mL) of alcohol in its body part in comparison with LB (408.0 mL) and UC (445.4 mL). In the descriptive analysis, UC had stronger intensities of sweet aroma, sour aroma and taste, and persimmon flavor (P<0.05). Persimmon characteristics seemed to be well characterized by UC. In conclusion, LD was the most efficient in terms of production cost, whereas UC would be used to produce a more flavorful sweet persimmon spirit.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.573-582
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• 2005

This study was conducted to investigate effects of the brown seaweed waste(BSW) supplementation on milk production and related endocrine response in serum in Holstein dairy cows. A total of 14 Holstein dairy cows(initial mean live weight 625kg, average lactation days 225, Reproduction 2.4) were randomly allocated into control(basal diet) and treatment groups (4% BSW/basal diet) with 7 replications for 90 days. Dry matter intake was not affected by brown seaweed waste supplementation, but daily milk yield(kg) at the last experiment significantly increased (6.25kg) in treatment group compared with control group(p<0.05) at the last experiment. The plasma insulin-like growth factor(IGF)-1, triiodothyronine($T_3$) and thyroxine($T_4$) levels were significantly increased in treatment group compared with control group(p<0.05), although the concentration of plasma growth hormone(GH) was not significantly different. Milk composition was not significantly different between groups. The somatic cell count(SCC) in milk were significantly reduced in treatment group compared with control group(p<0.05), but antibodies(total IgG, G1, G2) were not significantly different between groups. Therefore we strongly believe that the increased milk yield is related to metabolic hormones as IGF-1, $T_3$ and $T_4$ and the mechanism of reducing SCC in milk must do more study related nonspecific immunsystem in the future.

• Journal of Animal Science and Technology
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• v.52 no.5
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• pp.399-406
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• 2010

The objective of this study was to investigate the effects of dietary ginger and Scutellaria on antioxidant capacity and IgG concentration in plasma and colostrum of sows. Twenty-four sows (Landrace $\times$ Yorkshire) in parity 2 were assigned to 3 treatment groups (8 sows per treatment) from 30 day before farrowing up to weaning on day 21 after farrowing. The treatment diets included 1) CON (Basal diet), 2) GDP (0.3% ginger dry powder was used as the replacement of wheat in basal diet) and 3) SDP (0.3% Scutellaria dry powder was used as the replacement of wheat in basal diet). No differences were found in antioxidant capacity and IgG concentration in plasma of sows among all the three treatments. Antioxidant capacity in colostrum was not affected by replacement of ginger and Scutellaria dry powder. IgG and protein concentration in colostrum was significantly higher (P<0.05) in GDP than that in CON and SDP. However, fat and lactose concentration were not affected by treatments. The total amino acid concentration in colostrum was significantly higher (P<0.05) in GDP than that in CON and SDP. Plasma antioxidant capacity was not affected by treatments in piglet on 0 day and 21 day, but tended to increase by supplementation of ginger. On 0 day, plasma IgG in piglet was not detected by replacement of ginger and Scutellaria dry powder. However, plasma IgG concentration in piglets on 21 day was significantly higher (P<0.05) in GDP than that in CON and SDP. These results indicate that colostrum IgG concentration in sows and plasma IgG concentration in piglets were improved by feeding ginger dry powder 30 days before farrowing in comparison to basal diet.

• The Korean Journal of Pesticide Science
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• v.2 no.1
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• pp.46-52
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• 1998

The hydrolysis rate of insecticidal buprofezin(IUPAC : tert-butylimino-3-isopropyl-5-phenylperhydro-1,3,5-thiadiazin-4-one) in the range of pH 2.0 and 12.0 have been examined in 15%(v/v) aqueous dioxane at $45^{\circ}C$. The hydrolysis mechanism of buprofezin is proposed from the pH-effect, solvent effect(${\ell}{\gg}m$), thermodynamic parameter(${\Delta}H^{\neq}$=11.12 $Kcal{\cdot}mol^{-1}$ &, ${\Delta}S^{\neq}=5.0e.u.$), rate equation and hydrolysis product, l-isopropyl-3-phenyl urea. General acid catalyzed hydrolysis and specific acid catalyzed($k_{H3O+}$) hydrolysis through $A-S_{E}2$ and A-2(or $A_{AC}2$) reaction mechanism with orbital-control reaction proceed below pH 8.0 and above pH 9.0, the nucleophilic addition-elimination, $Ad_{N}-E$ mechanism via tetrahedral($sp^{3}$) intermediate is initiation by general base catalyzed($k_{H2O}$) reaction. Buprofezin was more stable in alkaline ($k=10^{-8}sec.^{-1}$) than acid solutions from the sigmoid pH-rate profile. And the half-life($t=\frac{1}{2}$) of hydrolysis reaction in neutral aqueous solution(pH 7.0) at $45^{\circ}C$ was about 3 months.

• Journal of Astronomy and Space Sciences
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• v.22 no.4
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• pp.393-408
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• 2005

We developed and compared two automatic algorithms for moving object detections in the YSTAR-NEOPAT sky survey program. One method, called starlist comparison method, is to identify moving object candidates by comparing the photometry data tables from successive images. Another method, called image subtraction method, is to identify the candidates by subtracting one image from another which isolates sources moving against background stars. The efficiency and accuracy of these algorithms have been tested using actual survey data from the YSTAR-NEOPAT telescope system. For the detected candidates, we performed eyeball inspection of animated images to confirm validity of asteroid detections. Main conclusions include followings. First, the optical distortion in the YSTAR-NEOPAT wide-field images can be properly corrected by comparison with USNO-B1.0 catalog and the astrometric accuracy can be preserved at around 1.5 arcsec. Secondly, image subtraction provides more robust and accurate detection of moving objects. For two different thresholds of 2.0 and $4.0\sigma$, image subtraction method uncovered 34 and 12 candidates and most of them are confirmed to be real. Starlist comparison method detected many more candidates, 60 and 6 for each threshold level, but nearly half of them turned out to be false detections.

• Korean Journal of Food Science and Technology
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• v.39 no.4
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• pp.438-446
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• 2007

The fermented soybean product, cheonggukjang, is favored by many people, partly due to its bio-functional ingredients. Since the fermentation process of cheonggukjang is mediated by enzymes, including proteases, produced by microbes, analysis of the proteome profile changes in cheonggukjang during fermentation would provide us with valuable information for fermentation optimization, as well as a better understanding of the formation mechanisms of the bio-functional substances. The soluble proteins from cheonggukjang were prepared by a phenol/chloroform extraction method, in order to remove interfering molecules for high resolution 2-D gel analysis. Proteomic analysis of the cheonggukjang different fermentation periods suggested that most of the soluble soy proteins were degraded into smaller forms within 20hr, and many microbial proteins, such as mucilage proteins, dominated the soluble protein fraction. The proteomic profile of cheonggukjang was very different from natto, in terms of the 2-D gel protein profile. Among the separated protein spots on the 2-D gels, 50 proteins from each gel were analyzed by MALDI-TOF MS and PMF for protein identification. Due to database limitations with regard to soy proteins and microbial proteins, identification of the changed proteins during fermentation was restricted to 9 proteins for cheonggukjang and 15 for natto. From de novo sequencing of the proteins by a tandem MS/MS, as well as by database searches using BLASTP, a limited number of proteins were identified with low reliability. However, the 2-D gel analysis of proteins, including protein preparation methods, remains a valuable tool to analyze complex mixtures of proteins entirely. Also, for intensive mass spectrometric analysis, it is also advisable to focus on a few of the interestingly changed proteins in cheonggukjang.

• Journal of Life Science
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• v.17 no.4 s.84
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• pp.462-469
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• 2007

In this study, we isolated, characterized, and compared the vasa homologous genes of diploid and triploid Paragonimus westermani and localized VASA homologous proteins in both lung fluke types. Open reading frames of Pw-vasa-2n and Pw-vasa-3n were of 1812 bp, and encoded deduced proteins of 622 amino acids with calculated molecular weights of 69.0 kDa and 68.9 kDa and pI's of 9.11 and 9.03, respectively. A comparison of these two VASA deduced protein sequences showed that only 6 of the 622 amino acids differed. The deduced sequences of Pw-VASA-2n and Pw-VASA-3n contained eight consensus sequences characteristic of the DEAD-box protein family and their N-terminal regions contained four arginine-glycine-glycine (RGG) motifs. These two lung fluke VASA-like proteins were more similar to those of other VASA proteins than to those of other DEAD-family proteins isolated from several organisms (planarian, zebra fish, mouse, and human). vasa homologous gene transcription and VASA protein expressions in triploid type lung flukes was slightly stronger than in the diploid type. Immunostaining showed that testes and a portion of the ovaries of both diploid and triploid lung flukes reacted strongly to anti-Pw-VASA antibody.