• Korean Journal of Ichthyology
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• v.27 no.1
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• pp.33-38
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• 2015

The diet composition of Leiognathus nuchalis were studied using 350 specimens collected from October 2010 to September 2011 in the coastal waters of Gadeok-do, Korea. L. nuchalis was carnivorous feeder that consumed mainly copepods, crab larvae, and shrimp larvae. Its diet also included small quantities of polychaetes, amphipods, euphausiids, bivalve larvae, and gastropod larvae. The size of L. nuchalis ranged from 3.0 to 9.8 cm in body length (BL). L. nuchalis underwent an ontogenetic dietary shift. 3~5 cm size individuals fed mainly on copepods. 5~7 cm size individuals fed mainly crab larvae and shrimp larvae. 8~10 cm size individuals fed mainly polychaetes. The size increases, the weight of food organisms are constantly increased.

• Journal of fish pathology
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• v.10 no.1
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• pp.65-72
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• 1997

In the present study, acute toxicity data were obtained for hydrogen sulfide to larvae and adults of blue crab Portunus trituberculatus, white shrimp Metapenacus monoceros and prawn Macrobrachium nipponense under continuous flow through system. The larvae 96hr-$LC_{50}$ values of hydrogen sulfide were 7.3, 9.3 and $9.0{\mu}g/\ell$ for P. trituberculatus, M. monoceros and M. nipponense respectively. The larval sensitivity of the three crustaceans studied for the hydrogen sulfide was in the order of P. trituberculatus> M. nipponense> M. monoceros. The adults 96hr-$LC_{50}$ values of hydrogen sulfide were 42.5, 37.8 and $56.6{\mu}g/\ell$ for P. trituberculatus, M. monoceros and M. ripponense, respectively. The order of toxicity of hydrogen sulfide to adults of the three crustaceans was P. tritruberculatus> M. monoceros> M. nipponense. The larval/adult ratios of hydrogen sulfide toxicity were 5.8, 4.1 and 6.3 for P. trituberculatus M. monoceros and M. nipponense respectively, and larvae were found to be more sensitive to hydrogen sulfide than adults in all cases.

• Journal of Korean Ophthalmic Optics Society
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• v.3 no.1
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• pp.9-13
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• 1998

Visual system of Korean Salamander(Hynobius leechi) was morphologically studied. Fertilized eggs in egg sacs were collected and were developed in sterile saline solution. Various sized larvae of 5-30mm in length were fixed. Specimens were paraffin sectioned and were observed under light microscope. In 5mm length larva, lens rudiment induced by optic cup was combined with sensory ectoderm. The shape of lens was changed as spherical in 12mm length larva, but the retinal layer did not differentiated into three layers. The differentiation of retinal layer was clear in 14-16mm length larva. The central region of lens fibers was degenerated. Iris and ciliary body were formed from the marginal zone of optic cup in 20mm length larva. Choroid was thicker in elder eye of 30mm length larva and cartilage developed at outer region of optic cup. The outer segment of photoreceptor cell layer grew longitudinally. Optic nerve was connected to the ventral part of brain through cartilage.

• Korean Aquaculture
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• v.14 no.2
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• pp.52-65
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• 2002

비단가리비의 인공 종묘 생산을 목적으로 전라남도 신안군 대흑산도 주변에 서식하는 비단가리비를 대상으로 인공 종묘 생산을 위한 산란 유발, 수정란의 발생 과정, 유생 사육, 채묘 및 중간 육성 등 양식 생물학적 연구를 실시하였다. 어미의 각종 산란 유발 자극에 대한 반응은 Serotonin 주사, 온도 자극, 혼합 자극에서 반응율이 가장 높았으며, 자외선 조사 해수 자극은 수컷만 반응을 하였고 간출 자극은 반응이 없어 실효성이 없는 것으로 나타났다. 수정란의 크기는 $69.5{\mu}m$이었고, 수정 후 약 2시간에 2 세포기, 8시간 후에 8 세포기, 20시간 후에는 담륜자 유생으로 부화하였으며 40시간 후에는 D상 유생으로 되었다. 수온별 비단가리비 유생의 성장은 수온 $20^{\circ}C$에서 각장 $178.9{\mu}m$로 가장 좋았으며, 이 때의 생존율은 15.5%이었다. 그러나 수온 $15^{\circ}C$에서는 낮은 성장을 보여 각장 $135.9{\mu}m$로 성장하였으며, 생존율도 9.8%로 저조하였다. 수용 밀도별 사육 시험에서 $1m{\ell}$당 1개체와 5개체에서 성장 및 생존율이 양호하여 성장 및 생존율을 볼 때 적정 사육 밀도는 $1m{\ell}$당 5개체 이하로 나타났다. 먹이 생물 종류에 따른 유생의 성장을 알기 위하여 I, gal-baba, C, calcitrans, N, oculata를 단독 또는 혼합으로 공급하였을 때 실험 종료후 각장의 성장은 I, galbana+C. calcitrans+N. oculata구가 $194.2{\mu}$로 가장 좋았고 N. oculata구는 $162.2{\mu}m$로 가장 낮은 성장을 보였다. 생존율에서는 I. galbana+C. calcitransoculata구는 9.4%로 가장 낮은 생존율을 보였다. 채묘기별 유생의 부착률은 염화비닐판을 수평으로 놓은 것과 패각이 각각 3.43%와 3.17%로 가장 양호하였으나, 양파망과 염화비닐판을 수직으로 놓은 것은 각각 1.52%와 1.61%로 비교적 저조하였다. 수정 후 40일째부터 90일째까지 측정한 부착치패의 경과 일수에 따른 각장의 성장은 $SL=184.44e^{0.0335X}(r^2=0.9861)$의 회귀직선식으로서 나타났다. 중간육성 시험에서 수심별 성장을 분석한 결과, 비단가리비 치패는 저층보다 표층이 각장 5.92mm, 전중량 6.07g 정도 더 빨리 성장하였다.

• Environmental Analysis Health and Toxicology
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• v.23 no.4
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• pp.337-343
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• 2008

따개비류는 암반기질에 무리를 이루어 비교적 단단히 부착생활을 하며 전 세계적으로 분포하는 생물이다. 본 생물은 형태가 다른 두 단계의 플랑크톤 유생시기를 거쳐 기질에 부착하여 생활한다. 따개비류에 관한 국내연구는 순수 분류학적으로 몇 편의 연구가 이루어졌을 뿐이며 실험실 내 사육조건 및 유생의 민감도 등에 관한 연구는 제대로 수행되어 있지 않은 실정이다. 본 연구에서는 첫 번째 단계 유생을 대상으로 몇몇 중금속(Cd, Cr, Cu 그리고 Zn)에 대한 민감도를 조사하였고 타 연구결과와의 비교로 생태 독성학적 적용을 시도하였다. 네 가지 금속물질, 카드뮴, 크롬, 구리 그리고 아연에 대한 따개비 유생의 반수사망농도는 각각 0.43, 24.4, 0.22 그리고 1.74mg/L로 산정되었다. 외국의 생물검정용 따개비와 본 연구에 사용된 고랑따개비의 민감도는 아주 유사하게 나타났다. 따라서 국내산 고랑따개비의 유생은 해양 환경 평가에 일반적으로 이용되는 해양생물과 동반 검정의 수행에 자격을 갖추었다고 할 수 있다. 본 연구의 결과는 해양환경의 평가는 물론 방오물질개발 등에의 기초자료를 제공할 수 있을 것으로 사료된다.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.14 no.3
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• pp.189-194
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• 2009

To investigate the difference in the ecological niches between Pfiesteria piscicida and Pfiesteria-like species (Cryptoperidininopsis brodyi and Stoeckeria algicida), we have observed the feeding behavior of three potential predators on abalone larvae and measured the survival rates of abalone larvae as a function of initial predator concentration. When the predators were mixed with abalone larvae, P. piscicida and C. brodyi became active and exhibited attacking behavior on abalone larvae within a few seconds. They could ingest whole soft body of abalone larvae using a peduncle. In contrast, feeding and attacking behavior were not observed from S. algicida. Survival rates in abalone larvae decreased with an increase of the initial concentration of P. piscicida and C. brodyi. These results indicate that Pfiesteria and Pfiesteria-like species displaying the very same shape and size have different ecological niche in the marine food webs, which implies that identification to species level is definitely important to understand and discriminate the ecological roles of them.

• 한국해양학회지
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• v.23 no.2
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• pp.70-75
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• 1988

In order to set up a predictive model for an effective spat collection of ark shells, Anadara broughtonii, the survival rate and the time required for each developmental stage of planktonic larvae were investigated during the period from July 1 to October 30 in 1974, in one of the main ark shell seed collection areas, Chinhae Bay, in the southern part of Korea. The advent of D-shaped larvae ca. $94.3{\times}72.7{\mu}m$ long had three peaks during the surveyed period: August 25, August 31 and September 9, umbo-shaped larvae ca. $141.6{\times}108.4{\mu}m$ and full grown larvae ca. $269.3{\times}221.7{\mu}m$ long also showed three peaks: September 6, September 12 and September 20 for the former, and September 20, September 25 and October 5 for the latter, respectively. About 11 to 12 days was required for D-shaped larvae to develop to umbo-shaped stage. At this intermorphological stage, the daily survival rate was 0.93 with a total survival rate of 45% for the stage. The time required for umbo-shaped larvae to develop to full grown larvae varied from 13 to 15 days with a daily survival rate of 0.93 and with a total survival rate of 36% in the period. Twenty-five to twenty-six days were required for each peak group of the D-shaped larvae to reach a full grown stage, and their total survival rate was 16% during this developmental stage.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.307-315
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• 2011

Digestibility index of 12 phytoplankton species were invested during the larval development sizes. Ingestible size of phytoplankton varied depending on larval sizes: Isochrysis galbana, I. aff. galbana, Pavlova lutheri, Chlorella ellipsoidea, Nannochloris oculata was ingested 94.2-99.7% all larval sizes. Cheatoceros calcitrans, C. gracilis and C. simplex could ingest over 90.0% after umbo stage (mean shell length $189.3{\pm}13.8{\mu}m$). Phaeodactylum triconutum, Dunaliella tertiolecta and Tetraselmis tetrathele could not ingested D-shaped larvae (shell length $65.0-100.0{\mu}m$) but ingested 97.3-99.7%, 43.3-99.3%, 48.5-99.3% after then larval stages, respectively. But Thalassiosira weissflogii was ingested 1.0-1.7% only at full grown stage. Over 50.0% ingestion cell size was D-shape stage larvae with smaller than mean $102.3{\mu}m$ in shell length could ingest phytoplankton with $4.6{\mu}m$ in both major and minor axis and up to $9.3{\mu}m$ in minor axis basis for larger than mean $158.3{\mu}m$ in shell length, respectively. At all larval stages, phytoplankton with larger than $10.0{\mu}m$ in both major and minor axis could not be ingested.

• The Korean Journal of Malacology
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• v.25 no.2
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• pp.97-103
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• 2009

It is a research that free floating larva of Ruditapes philippinarum in compliance with infiltration flow of tidal accumulation and bottom-clinging system in high density also, It is a precondition this for the continuous augmentation of Ruditapes philippinarum resources. So in flow field of tidal the free floating larva produced the infiltration water tank that can possibly bottom-clinging and experiment the water tank which uses free floating larva, It evaluates that the acceleration effect of free floating larva by infiltration in objective. As a result, 1) The experiment on free floating larva's bottom sediment grain diameter came to be high recording as bottom-clinging rate at the static water field and even biologically it selects bottom-clinging substrate it will be able to confirm. 2) About occurrence of infiltration flow field is in case of that: the drift of a current 10cm/s, bottom sediment grain 1.21mm infiltration flow 0.3cm/s increase of 3~5 times was confirmed. 3) From free floating larva of Ruditapes philippinarum the choice of bottom sediment grain diameter depends in the biological factor and form the flow field the bottom-clinging acceleration effect was controled over physical stable condition. 4) In case of density of Ruditapes philippinarum free floating larva of sea area, bottom sediment grain diameter, flow condition which are very cleanly in the research that possibly could conjecture the free floating larva's bottom-clinging rate.

• The Korean Journal of Malacology
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• v.17 no.2
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• pp.79-84
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• 2001

In order to obtain the basic information for seedling production of the scallop, Patinopecten yessoensis, the egg development and larva growth were investigated at different conditions such as water temperature, salinity and phytoplankton. Eggs were demersal isolated eggs, which averaged 77.3${\pm}$2.7$\mu\textrm{m}$ in diameter after spawning. The fertilized eggs developed to D-shaped larva of shell length 117.5${\pm}$3.8$\mu\textrm{m}$ after 60 hours at 15$^{\circ}C$. The range of water temperature and salinity during egg development were 10-20$^{\circ}C$ and 28-34 ppt, respectively. The time of egg development was shorter with higher water temperature. After 10 days of spawning, D-shaped larva reached 160 $\mu\textrm{m}$ in shell length, and after 25 days became full-grown larva 250 $\mu\textrm{m}$ in shell length, in which could be observed eye spots. The relative growth formula between shell length (SL) and shell height (SH) was SH = 1.0425SL-27.731 (r$^2$= 0.9749) during the entire larva period. In regard to water temperature, growth and survival rates of larvae were good at 16$^{\circ}C$. Lower growth and survival rates were observed at 12$^{\circ}C$ and 20$^{\circ}C$ than that at 16$^{\circ}C$. When larvae were fed mixed phytoplankters, such as isochrysis galbana, Pavlova lutheri and Chaetoceros calcitrans, their growth and survival rates were the highest among groups.