• Korean Journal of Veterinary Research
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• v.37 no.1
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• pp.185-188
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• 1997

전남대학교 수의과대학에서는 조류 blue-green algae를 먹이로 하여 간질의 중간숙주인 애기물달팽이를 실험실에서 유지시켜 왔으나 조류의 배양에 어려움이 많아서 보다 간편하면서 달팽이의 번식에도 좋은 방법을 고안하고자 노력하였다. $28.5{\times}17{\times}18cm$ 크기의 유리수조에 모래를 1.5cm 높이로 깔고 10개의 꼬막껍질을 그 위에 올려놓은 다음 4리터의 증류수를 부어넣었다. 야외에서 채집한 달팽이의 난괴를 부화시켜 얻은 0.63cm 크기의 어린 달팽이 45마리를 세 그룹으로 나누어 세 개의 수조에서 $20^{\circ}C$의 온도를 유지하면서 사육하였고 먹이로 상추잎을 넣어주었다. 매주 수조바닥의 물 $2{\ell}$를 흡인하여 갈아주면서 상추잎을 바꿔넣었고, 계속해서 공기를 통과시켜 산소를 공급하였다. 이와 동일한 방법으로 실험실에서 조류로 사육하던 17대의 달팽이를 사육하여 그 성적을 비교하였던 바 야외에서 채집한 달팽이의 성장률이 실험실에 적응된 것들에 비하여 약간 떨어졌으나 생존율이나 산란율에서는 큰 차이가 인정되지 않았다. 그래서 1년이 넘도록 이 방법으로 달팽이를 사육하면서 간질의 피낭유충을 생산하고 있다. 꼬막껍질은 서서히 분해하면서 달팽이의 각을 형성하는 물질을 제공하여 생존율을 높게 한 것으로 생각된다. 그리고 수조를 햇볕이 드는 창문 가까이에 놓아두었기 때문에 조류가 자생하여 적은 양이나마 달팽이의 먹이가 되었을 것으로 보인다.

• Journal of Aquaculture
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• v.4 no.1
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• pp.1-12
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• 1991

A series of rearing experiments were conducted to determine the growth rates and feed conversion efficiencies of tilapia in accordance with body size or age in nearly total closed system glass aquariums ($270\;\ell$ each in water volume) and concrete tanks ($4000\;\ell$) from April 10 to October 16, 1987. The fish used for the experiments was a Japanese strain of Oreochromis niloticus, and the size of the fish ranged from 7 g to more than 1,000 g in body weight. The starting stocking rates for each experimental lot were 10 to 20 kg in the glass aquarium ($3.7{\%}$ to $7.4{\%}$ of water volume) and 200 kg in the concrete tank ($5{\%}$ of water volume). A single experimental rearing term was 14 days with slight variations on occasions. Water temperature was designed to be kept at $26^{\circ}C$ but slight fluctuations were inevitable. Dissolved oxygen level was designed to be maintained at around $3\;mg/\ell$, but it also showed some variations. The ammonia level in the glass aquarium section once reached up to $18\;mg/\ell$, but generally remained at around $4\;mg/\ell$, and in the concrete tank section it was maintained at around $1\;mg/ell$. The feed was composed of mainly soybean meal with a small amount of fish meal as the protein source, and the crude protein content was about $32{\%}$. Mean daily growth rate was $3.5{\%}$ of body weight with 0.9 in food conversion ratio in the glass aquarium when the mean weight of fish was around 10 g with gradually reduced performances as the fish grew bigger. When the mean weight was 800 g, mean daily growth rate was $0.5{\%}$ with about 1.5 in food coversion for fish in the glass aquarium, and $0.8{\%}$ and 1.6 for fish in the concrete tank, respectively. According to the mean growth rate obtained from this experiment, it was calculated that the fish reared in the concrete tank require 223 days from 50 g to reach 1,000 g which is the ideal size for market in Korea, at the conditions provided as above, and 302 days from 10 g fingerlings to 800 g fish in the glass aquarium conditions of the closed recirculating water system.

• Journal of Advanced Marine Engineering and Technology
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• v.37 no.6
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• pp.623-630
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• 2013

This study was to evaluate hydrodynamic performance evaluation between an abroad product, a developed inflatable kayak and new developed kayaks. In order to test, inclining and turning trial test were carried out in the Ocean engineering Basin. Also, resistance test was carried out using a reduced scale model in the circulating water channel. In conclusion, stability of KONA was evaluated was the most greatest, the coefficient of resistance and center of gravity from RD-FK-12 were considerable, and turning performance of RD-FK-11 was greater than this of KONA and RD-FK-12.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.10a
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• pp.293-294
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• 2001

전복류는 옛날부터 고급수산품으로서 가치가 높고, 근년에 와서는 경제성장과 함께 그 수요가 날로 증가되는 추세로 양식 대상종으로 전망이 밝다(변 등, 1984, 김, 1997). 전복양식은 최근 연안환경의 오염으로 인한 양식장 적지의 제한 등의 여러 가지 문제점의 제기로 종묘생산과 양성에 이르기까지 육상 수조에서 완전양식이 시도되고 있는 추세이다. 육상수조식 양식은 전 사육과정을 인위적으로 관리 하면서 사육하기 때문에 생존율 및 성장효과 면에서는 다른 양식 방법보다 유리하다는 이점을 가지고 있지만, 전복이 한정된 공간에서 대량으로 사육됨에 따라 여러 가지 질병과 수질악화, 여름철 고수온기 때의 수온의 급상승이나 적조 생물 및 이상수괴에 의한 치패의 성장저하 대량 폐사 등의 문제점을 가지고 있다. (중략)

• The Korean Journal of Malacology
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• v.30 no.3
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• pp.235-242
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• 2014

This study was conduct to investigate the effect of Intermediate culture types on the growth and survival rate of the abalone, Haliotis discus hannai, in net cage and indoor tank. Intermediate cultures were to determine there that was to setting at marine net cage culture (NCC) in net cage, floor culture (FC), net floor culture (NFC), double shelter culture (DSC) and indoor net cage culture (INCC) in indoor tank, in two replicate. In the growth performance of juvenile abalone reared through intermediate culture, that the absolute growth rate ($AGR_{SL}$, $AGR_{SB}$), daily growth rate ($DGR_{SL}$, $DGR_{SB}$), and specific growth rate ($SGR_{SL}$, $SGR_{SB}$) to the shell length $(_{SL})$ and shell breadth $(_{SB})$ of NCC were higher than those of different groups (P < 0.05). As weight gain (WG), daily weight gain (DWG) and specific weight gain (SWG) to body weight through intermediate culture types in indoor tank was not significant. Also that, survival rate among experimental groups of intermediate culture in indoor tank was not significant. Therefore, these results is showed that should to cultivate for net cage so that intermediate culture of juvenile abalone over 2 cm, accordingly research to effective progress of juvenile abalone intermediate culture in indoor tank be should from various reason as well as feed and rearing condition.

• Journal of Ocean Engineering and Technology
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• v.9 no.2
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• pp.61-70
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• 1995

The purpose of this paper is to calculate the excavation volume of unequal interval grid using nonlinear boundary in eathwork volume determination for reclamation of the foreshore. A congruence area formula by first and third equation is compared with trapezoidal, simpson formulas to earthwork volume. And nonlinear spot level method of unequal interval grid is compared with linear and nonlinear spot level method of equal interval grid excavation volume. As a result algorithm of derived area and volume formula should provide a better accuracy than linear and nonlinear spot level currently in use. Practical application of each method to the excavation volume is illustrated by digital elevation model of aerial photogrammetry and model test of aquarium.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.40 no.4
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• pp.304-310
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• 2004

Resistance performance of small size fishing vessel is experimentally investigated to find out the effect of fore-body shape and trim for the better hull from development. The tests are performed for five different cases in the high speed circulating water channel(CWC). Wave patterns are observed to investigate the relation between the resistance performance and the wave characteristics.

• KSBB Journal
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• v.24 no.5
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• pp.432-438
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• 2009

Here, we demonstrated simple nucleic acid, RNA, concentration method using polymer micro chip containing glass bead ($100\;{\mu}m$). Polymer micro chip was fabricated by PDMS ($1.5\;cm\;{\times}\;1.5\;cm$, $100\;{\mu}m$ in the height) including pillar structure ($160\;{\mu}m\;(I)\;{\times}\;80\;{\mu}m\;(w)\;{\times}\;100\;{\mu}m\;(h)$, gap size $50\;{\mu}m$) for blocking micro bead. RNA could be adsorbed on micro glass bead at low pH by hydrogen bonding whereas RNA was released at high pH by electrostatic force between silica surface and RNA. Amount of glass beads and flow rate were optimized in aspects of adsorption and desorption of RNA. Adsorption and desorption rate was measured with real time PCR. This concentrated RNA was applied to amplification micro chip in which NASBA (Nucleic Acid Sequence Based Amplification) was performed. As a result, E.coli O157 : H7 in the concentration of 10 c.f.u./10 mL was successfully detected by these serial processes (concentration and amplification) with polymer micro chips. It implies this simple concentration method using polymer micro chip can be directly applied to ultra sensitive method to measure viable bacteria and virus in clinical samples as well as environmental samples.

• Journal of Marine Life Science
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• v.7 no.2
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• pp.139-144
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• 2022

This study aims to investigate egg quality changes and free amino acid composition. Factors that can affect egg quality about farming conditions were investigated through biochemical analysis of egg by red spotted grouper, Epinephelus akaara. As a result, the buoyant, fertilization embryonic survival and hatching rate were better when reared in sea cage than in tank. And the content of free amino acid in fertilized eggs reared in sea cage was higher then in tank. In conclusion, fertilized eggs with good egg quality can be produced when farming sea cage, and contained more free amino acid.

• The Korean Journal of Pharmacology
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• v.31 no.1 s.57
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• pp.135-140
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• 1995

Glycation occurs by covalent binding between the carbonyl group of monosaccharides and the epsilon amino group of amino acid. It can alter the physiological function of proteins and causes the development of diabetic complications. In this study, the influence of glycation on protein binding of warfarin and dansylsarcosine was studied by equilibrium dialysis which was performed for 3 hours at $37^{\circ}C$ in the water bath. The high glycated albumin which contained $50{\pm}16%$ of glycated albumin bound less than natural albumin which contained $8.5{\pm}5.28%$ of glycated albumin, if drugs concentration were more than the albumin concentration. But only warfarin binding showed a significant difference of 6% (P<0.05) when the molar concentration ratio of warfarin per albumin was 3. In consideration of low therapeutic concentrations, low glycated albumin concentrations in the body, and rapid elimination of excessive free drugs, these small increaes of free warfarin concentrations by glycation of albumin are not considered as risk. factors for drug intoxication for diabetics, if renal functions are intact.