• Nuclear Engineering and Technology
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• v.5 no.3
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• pp.202-213
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• 1973

In a continuation of the 1st series of these studies, a separation of the components in organic phases obtained from Szilard Chalmer reactions of aromatic chloro derivatives was attempted to clarify the conditions of isolating the main component. Though it was unable to isolate all constituents of organic phases, the main component was well isolated by means of a simple distillation or a thin layer chromatography. It was identified that the main component was a recombined product of the recoiled $^{38}$ Cl atom with the original sample. In cases of solid samples the main component amounted about 80-60% of the radio activity of the organic phase, whereas the main component from liquid samples gave less than 70% of the radio activity of the organic phase. A prolonged irradiation could increase the yield of a main component, but it was, in case of a radiation senintive compound, decreased as the irradiation is extended. The number of aide produets separated from an organic phase of a liquid sample was greater than that of a solid sample, and hence the main component of a solid sample was isolated easier. The overall fields of the labelled main products of 22 kinds of compounds were listed and a procedure of the separation of the main component by a thin layer chromatograply was proposed and evaluated. The feasibility of the present procedure for labelling purpose was also reviewed with respects to the chemical structure of a compound.

• Korean Journal of Plant Tissue Culture
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• v.28 no.3
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• pp.165-171
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• 2001

New visible and fast assay system have been developed for tobacco transformant introduced with adenosine deaminase (ADA) marker gene, which converts cytotoxic adenosine analogues to non-toxic inosine analogues and ammonia. Ammonia was changed to blue color in the solution of phenol-nitoprusside and alkaline-hypochlorite. It was possible to detect activity of ADA visibly on the holes of 96 well plate using tiny explant of transgenic tobacco leaves within 1 hour incubation time. As substrates of ADA enzyme from transgenic plant on the plate, a number of adenosine analogues such as 9-D-arabinofuranosyl adenine, cordycepin, 2'-deoxyadenosine, adenosine and xylofuranosyl adenine were possible for detection of ADA activity. Optimal condition of substrate for ADA enzyme was each 10 mM and pH 7.5 in adenosine solution. Especially, transgenic plant did not convert adenosine to inosine and ammonia in the presence of ADA inhibitor deoxycoformycin, which means that ammonia produced from transgenic plant is due to expression of ADA gene. Now, we show that this detection system can be easily, sensitively, fast and cheaply as well as visibly assayed in vitro as GUS gene system with very small size of transformant explant.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.1
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• pp.75-85
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• 2023

This study was conducted to assess the effect of heptapeptide, composed of seven amino acids, on the activation of human hair cells isolated from human hair follicles. We have confirmed that the heptapeptide could bind to Lgr5 from the results of surface plasmon resonance (SPR) analysis. Heptapeptide enhanced the proliferation of human hair follicle dermal papilla cells (HHFDPCs) in a dose dependent manner. It induced the protein level of nuclear β-catenin, and the expressions of β-catenin downstream target genes, including LEF1, Cyc-D1 and c-Myc, in HHFDPCs. Heptapeptide significantly induced the phosphorylation of Akt and ERK, and the mRNA expressions of growth factors, including hepatocyte growth factor (HGF), keratinocyte growth factor (KGF) and vascular endothelial growth factor (VEGF), in HHFDPCs. In addition, heptapeptide significantly increased mRNA expression levels of differentiation-related transcription factors of human hair germinal matrix cells (HHGMCs) and differentiation markers of human hair outer root sheath cells (HHORSCs). Additionally, we investigated the effect of heptapeptide on human hair follicle stem cells (HHFSCs) differentiation and found that the heptapeptide reduced the mRNA and protein levels of stem cell markers, while it increased those levels of differentiation markers. These results have indicated that the heptapeptide promotes proliferation or differentiation of various types of hair follicle constituent cells through the induction of Wnt/β-catenin signaling. From the results, we have suggested that the heptapeptide in this study could be applied as a new functional material for the improvement of hair growth and alopecia.

• Journal of the Korean Society of Hazard Mitigation
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• v.5 no.3 s.18
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• pp.67-72
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• 2005

This study is intended to examine the effect of photoluminescent exit path markings in the event of failure of both the power and back-up power to the lighting and illuminated exit sign. To achieve the purpose, the test house was exhibited in Fire EXPO '05. 520 visitors were examined from May 26-29, 2005. The results of this study are as follows; The evacuation from buildings in dark conditions showed that 70% of men and 72% of women were crawled along the wall. Meanwhile, 88% of men and 83% of women were evacuated with ordinary walking in photoluminescent exit path markings. The photoluminescent exit path markings located on public buildings floors will aid in evacuation from buildings in the event of failure of the power to the lightings and illuminated exit signs.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.232-232
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• 2004

본 연구는 돼지의 발정동기화에 의한 과배란 유기시 돼지 난소의 배란상태(황체수)와 혈액내 혈액성분(WBC, RBC Hb, HCT, MCV, MCH, MCHC, Platelet)과의 비교분석을 통하여 다배란에 따른 표지(Marker) 혈액성분을 구명하기 위하여 실시하였다. 돼지 난소의 배란상태를 구명하기 위하여 30두의 성축 암컷을 이용하였으며, 발정 동기화는 PG 600(PMSG 400 IU, HCG 200IU)을 주사한 후 10일 후에 Regumate porcine을 6일간 급여한 후에 PMSG 주사하고 2일 후에 hCG주사한 다음날 자연종부를 실시하여 수정에 이르게 하였다. (중략)

• Product Safety
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• s.198
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• pp.38-41
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• 2010

'전기 없이도 작동하는 피난유도선', '와이어리스(무선) 피난유도선' 바론 (주)알지비테크롤러지(회장 조성복)사의 제품이다. 발광시트 전문생산업체인 알지비테크놀러지는 그 동안 알지비테크놀러지는 발광시트를 이용하여 기존 비상등 및 축광 표지판등이 가지고 있는 문제점들인 외부충격에 의한 파손, 짙은 연기에서의 시인성(연기속 시각효과)과 유도능력 등을 보완할 수 있는 제품 개발에 힘써왔다. 지난2010. 5.12 ~ 5.14간 이뤄진 '2010 재난대응 안전한국훈련' 중 지하철화재 대응훈련부분에 참가하기 위하여 '와이어리스피난유도선'을 몽촌토성역 설치로 그 첫 선을 보였는 바, 국내외 재난안전관련 업계로부터 최고의 찬사로 주목을 받았고, 이에 제품관련 문의와 상담이 폭주하고 있어 세계적으로 주목받는 기업으로의 성장을 기대하고 있다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2018.11a
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• pp.141-143
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• 2018

세계적으로 해양자원의 개발, 해양과학자료 조사 등 다양한 목적으로 해양구조물은 증가되고 있다. 해양구조물의 안전과 선박의 충돌을 방지하기 위하여 IALA, IMO등 국제기구에서는 해양구조믈에 등부표, 등대, 전기폰, 레이콘 등 해양안전유도장비의 설치를 권고하고 있다. 해양안전유도 장비의 설치 목적을 달성하기 위하여는 안정된 전기 에너지의 공급이 필요하다. 고립된 해상에서의 전력을 공급하기 위하여는 태양광, 풍력 등 신재생에너지를 사용하여 전력을 생산할 때, 그간 에너지원으로 사용하지 않던 일정 전압 이하의 에너지를 최대한 충전 가능전압으로 승압하는 에너지 수확(Energy Harvest)형 충방전조절기 개발 기술을 연구하였다.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.524-527
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• 2009

Spontaneously infected and non-infected dairy cows were assessed in a cross-sectional study aimed at determining whether bovine leukocyte markers may diagnose intra-mammary infections (bovine mastitis). Animals located in herds where bovine mastitis was highly prevalent were investigated (n = 31 animals). The expression of three cell-surface markers (CD11b, CD4 and CD8) was assessed, and the somatic cell count (SCC) and bacteriological analyses (both cultures and PCR tests) were also conducted. Cows identified as infected revealed statistically significant higher milk leukocyte CD11b, CD4 percentage and milk CD4/CD8 ratios than non-infected cows. Immunological markers may diagnose spontaneous bovine mastitis.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.22 no.5
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• pp.148-162
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• 2023

The objective of this study is to derive the appropriate perception location for changes in driving behavior of autonomous vehicles in urban road environments based on traffic safety signs. For this purpose, 32 types of signs that induce changes in driving behavior were selected from currently used traffic safety signs and classified as three types according to changes in driving behavior. Based on this, three scenarios were designed: stop, speed change, and lane change scenarios. These were used to confirm the impact on traffic flow. As a result of the analysis, it was found that each scenario needs to receive information on traffic safety signs in advance to ensure changes in traffic flow and safety. Consequently, the appropriate perception location can be used as a basis for establishing standards for delivering message sets to autonomous vehicles or revising traffic safety signs for them. In addition, this study is expected to contribute to the establishment of safe and efficient driving strategies on urban roads as autonomous vehicles are introduced in the future.

• Radiation Oncology Journal
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• v.24 no.2
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• pp.128-137
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• 2006

Purpose: We investigated whether a relationship exists between tumor control dose 50 ($TCD_{50}$) or tumor growth delay (TGD) and radiation induced apoptosis (RIA) in syngeneic murine tumors. Also we investigated the biological markers that can predict radiosensitivity in murine tumor system through analysis of relationship between $TCD_{50}$, TGD, RIA and constitutive expression levels of the genetic products regulating RIA. Materials and Methods: Syngeneic murine tumors such as ovarian adenocarcinoma, mammary carcinoma, squamous cell carcinoma, fibrosarcoma, hepatocarcinoma were used In this study. C3H/HeJ mice were bred and maintained in our specific pathogen free mouse colony and were $8{\sim}12$ weeks old when used for the experiments. The tumors, growing in the right hind legs of mice, were analyzed for $TCD_{50}$, TGD, and RIA at 8 mm in diameter. The tumors were also analyzed for the constitutive expression levels of $p53,\;p21^{WAF1/CIP1},\;BAX,\;Bcl-2,\;Bcl-X_L,\;Bcl-X_S$, and p34. Correlation analysis was peformed whether the level of RIA were correlated with $TCD_{50}$ or TGD, and the constitutive expression levels of genetic products regulating RIA were correlated with $TCD_{50}$, TGD, RIA. Results: The level of RIA showed a significant positive correlation (R=0.922, p=0.026) with TGD, and showed a trend to correlation (R=-0.848), marginally significant correlation with $TCD_{50}$ (p=0.070). It indicates that tumors that respond to radiation with high percentage of apoptosis were more radiosensitive. The constitutive expression levels of $p21^{WAF1/CIP1}$ and 34 showed a significant correlation either with $TCD_{50}$ (R=0.893, p=0.041 and R=0.904, p=0.035) or with TGD (R=-0.922, p=0.026 and R=-0.890 p=0.043). The tumors with high constitutive expression levels of $p21^{WAF1/CIP1}$ or p34 were less radiosensitive than those with low expression. Conclusion: Radiosensitivity may be predicted with the level of RIA in murine tumors. The constitutive expression levels of $p21^{WAF1/CIP1}$ or p34 can be used as biological markers which predict the radiosensitivity.