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Leukocyte Markers Differentiate Non-Infected from Spontaneously Infected Dairy Cows  

Yu, Do-Hyeon (College of Veterinary Medicine, Chonbuk National University)
Lee, Jong-Hyeon (College of Veterinary Medicine, Chonbuk National University)
Song, Ru-Hui (College of Veterinary Medicine, Chonbuk National University)
Noh, Dong-Ho (College of Veterinary Medicine, Chonbuk National University)
Li, Ying-Hua (College of Veterinary Medicine, Chonbuk National University)
Lee, Mi-Jin (College of Veterinary Medicine, Chonbuk National University)
Park, Jin-Ho (College of Veterinary Medicine, Chonbuk National University)
Publication Information
Journal of Veterinary Clinics / v.26, no.6, 2009 , pp. 524-527 More about this Journal
Abstract
Spontaneously infected and non-infected dairy cows were assessed in a cross-sectional study aimed at determining whether bovine leukocyte markers may diagnose intra-mammary infections (bovine mastitis). Animals located in herds where bovine mastitis was highly prevalent were investigated (n = 31 animals). The expression of three cell-surface markers (CD11b, CD4 and CD8) was assessed, and the somatic cell count (SCC) and bacteriological analyses (both cultures and PCR tests) were also conducted. Cows identified as infected revealed statistically significant higher milk leukocyte CD11b, CD4 percentage and milk CD4/CD8 ratios than non-infected cows. Immunological markers may diagnose spontaneous bovine mastitis.
Keywords
Bovine mastitis; Flow cytometry; CD11b/CD4/CD8;
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