• Journal of Soil and Groundwater Environment
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• v.13 no.3
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• pp.59-66
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• 2008

A biological study was conducted to evaluate the enhancement of landfarming of soil contaminated with petroleum hydrocarbon (TPH) applying organic composite nutrients and a chemical oxidation during bioremediation. The target value of soil TPH after treatment was 500 mg/kg TPH. Addition of an organic compost and liquid swine manure for the removal of soil THP showed higher efficiency as 84.4% and 92.2% respectively than inorganic nutrients of 80.2%. In addition to the removal of non-biodegradable portion of residual hydrocarbons in soil, a chemical oxidation was applied during tailing period of the biological remediation, which showed high remediation efficiency as 98.1% compared with single bioremediation efficiency of 84.7%.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.487-487
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• 2023

농업 분야에서 미생물은 영양분 가용화, 유기물 분해 등 토양 영양분 공급에 중요한 역할을 하며, 토양 건강성 증진, 식량 안보 및 식품 건강 면에서 많은 활용 가능성을 지니고 있다. 최근 유역 환경 건강성, 생물 다양성 보존, 효율적인 고품질 농산물 생산에 대한 관심이 커져, 지속 가능한 농업 중 하나인 유기농업과 관행농업 토양의 이화학적 및 생물학적 특성에 관한 비교 연구가 진행되고 있다. 미생물은 지속 가능한 농업 발전의 중요한 요소 중 하나로써, 미생물 다양성이 풍부할수록 토양 비옥도, 작물 성장 면에서 긍정적인 영향을 미친다고 알려져 있다. 본 연구는 이에 대한 기초 데이터를 제공하기 위해 논 경작지를 대상으로 유기 및 관행농업 토양의 미생물 군집조성과 Alpha diversity analysis(Chao1, Shannon, Simpson index)을 통해 비교하였다. 경기도 양평군에서 유기 및 관행 논 지역을 각각 1지점씩 선정하였으며, 8월부터 11월까지 총 4회 현장 조사를 진행하였다. 미생물 분석은 차세대염기서열분석을 실시하였으며, bacteria는 16S rRNA V3-4 영역, fungi는 ITS 3-4 영역을 sequencing 하였다. 미생물 군집조성은 문수준에서는 큰 차이가 없었으나, 속수준에서는 fungi 군집조성에 차이를 보였다. 예로 Ustilaginoidea 속은 관행 논 토양에서만 발견되었으며, 벼 이삭누룩병을 일으키는 병원균으로 과도한 질소 비료 시비가 원인으로 추정된다. 종 다양성은 bacteria diversity의 경우 관행 논 토양에서 높게 측정되는 반면, fungi diversity의 경우 유기 논 토양에서 높게 측정되었다. 결론적으로 체계적인 시비 관리 통해 미생물 군집은 조절될 수 있으며, 관행농업은 적절한 시비를 통해 토양 건강성 및 식품 건강성 면에서 유기농업과 비슷한 효과를 보여줄 가능성이 있다고 판단된다.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.3
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• pp.67-74
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• 2003

Starvation promoters can be utilized for in situ bioremediation and for the efficient bioprocessing. Previously we have cloned and characterized strong starvation promoters from envrionmentally relevant bacteria, Pseudomonas putida strains (Y. Kim, and A. Matin, J. Bacteriol. 177:1850-1859, 1995). Here we report the construction of the plasmid pYKS101 using one of the starvation promoters from P. putida MK1. The pYKS101 was found to be useful for a novel starvation promoter-driven gene expression system. Under this system, the target reporter gene, lacZ, was stably integrated into the chromosomal DNA of P. putida MK1. ${\beta}$-galactosidase activity was found to be four-fold higher upon carbon starvation than during exponential growth. The resultant recombinant strain is indigenous to the environment contaminated with various toxic materials, hence can be a good candidate for in situ bioremediation.

• 월간낙농육우
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• v.27 no.9 s.305
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• pp.172-178
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• 2007

우리나라의 젖소 사육농가도 예전과는 달리 효율적인 사양관리를 기하기 위해 부단히 노력한 결과, 조사료와 농후사료의 배합비율이나 사료의 질과 양을 어느 정도로 급여하는 지를 사육농가가 관심을 가지는 농가가 늘고 있는 추세이다. 특히 젖소사양관리에서는 고품질우유, 고능력우가 증가하면서 우유의 생산성, 고능력우의 선발육종, 송아지의 조기육성시스템, 분만간격단축 등에 현저한 향상을 보이고 있다. 젖소는 건유기의 조사료위주의 사양으로부터 비유최고조기에 농후사료위주 사양에 이르면서 사료의 급격한 변화에 따라 짧은 기간내에 적응하지 않으면 안된다. 이러한 이유로 인해서 영양불량응로 인한 대사성질병들의 발생이 증가하고 있는 실정에 비추어 소의 생리적조건에 맞는 필요한 영양분을 충분하게 공급하여 주지 않으면 안된다.

• Applied Chemistry for Engineering
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• v.29 no.1
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• pp.1-9
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• 2018

Microalgae is considered as one of environmentally sustainable and potential feedstocks to produce biodiesels. However, recent studies on life cycle assessments (LCA) of microalgal buidiesels have shown that energy requirement is not small to produce biodiesel from microalgae, especially during cultivation stage. The costs for carbon sources, nutrients like nitrogen or phosphorous, and water for cultivation can contribute up to 80% of the total medium costs. In the present article, recent trends on the utilization of several promising nutrient sources such as municipal wastewaters, organic fertilizers, combustion exhaust emissions and organic solid wastes were reviewed, and the potential strategies to be used as substitutes of artificial culture media, especially for the biodiesel production, were discussed.

• Journal of the Korea Organic Resources Recycling Association
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• v.10 no.2
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• pp.100-116
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• 2002

There are several purpose to introduce microorganism into the Soil. The major purpose is to promote plant growth and inhibit plant pathogens. The model example is to put in nitrogen fixing symbiotic bacteria, Pythium and Rhizobium. In order to achieve the intended goal, the introduced microorganism should survive and colonize with sufficient density. The survival of introduced microorganism depend upon biotic and abiotic factors. Predation and competition are important among biotic factor. Water tension, organic carbon, inorganic nutrients(N, P), pH are important factor among abiootic factor. Soil texture and distribution of soil pore are also important in the survival and colonization of introduced microorganism. Selection by soil ecosystem for inoculant is a crucial factor for colonization. Good example are control of autochtonous microorganism and the introduction of surfactant biodegrading Pseudomonas. Sometimes, carriers such as peat and montmorillonite can be added to help colonization. Carriers can protect introduced microorganism by supplying protective microhabitat. Organic polymer is also used as a carrier to immobilize bacteria or industrial enzymes. Examples of these carrier are calcium alginate, agarose and k-carrageenan. The function of these carrier is to provide microhabitat and help colonization for introduced microorganism.

• Geotechnical Engineering
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• v.14 no.4
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• pp.151-162
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• 1998

The compressibility and the permeability of organic soil are so high that they cause many engineering problems when constructing a structure on the soil. If the organic content of the soil could be reduced by any applicable engineering technique, the engineering properties of the soils can be improved to some extent. The purpose of this study would thus be focused on how to decrease the amount of organic matters by applying aerobic biodegradation for eliminating post-construction settlement problems. To enhance the aerobic decomposition, oxygen was supplied to the soil samples prepared by the mixture of kaolinite and sawdust as organic matter. The dissolved oxygen and the organic content of the soil samples were measured, in accordance with the passage of time through the bests. As oxygen suppliers, HaOa liquid and pure oxygen gas were compared to meet the requirement of the test purposes. Newly manufactured oedometer with the diameter of 130 mm and the height of 300 mm was used for 100 days to perform the compressibility tests for the soils. Based on the results of this experiment, the oxygen gas-treated samples with nutrient settled 30% more than the samples untreated. This confirmed the efficiency of the aerobic biodegradation. $NaNO_3$ added into the soils as nutrients was proved more effective than $K_2HP0_4$. To confirm the activity of micro-organisms, sodium azide was also added to the soils.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.5
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• pp.927-938
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• 2000

When microorganism is injected into porous medium such as soils along with appropriate substrate and nutrients, biomass retained in the soil pore. Soil pore size and shape are varied from the initial condition as a result of biofilm formation, which make hydraulic conductivity reduced. In this research, hydraulic conductivity reduction was measured after microorganism are inoculated and cultured with synthetic substrates and nutrients. Biomass-soil mixture was evaluated its applicability to the field condition as an alternative liner material in landfill by measuring hydraulic conductivity change after repetitive freeze-thaw cycles. Resistance of biofilm to chemical solution and degree of biodegradation were measured through column test.

• Journal of Animal Environmental Science
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• v.17 no.1
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• pp.49-54
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• 2011

Animal manure is produced annually 43.7 million tonnes in Korea. Among them, about 85.6 % are used as compost or liquid fertilizer to the agricultural land. The animal manure can be effectively utilized by mixing with organic byproducts that result in generation of biogas from anaerobic co-digestion process. This study aimed to optimize the content of total solid materials (TS) and determine the effect of organic byproduct on the co-digestion process. Prior to the byproduct treatments, determination of proper content of TS was conducted by controlling at 5 or 10 %. For the byproduct treatments, swine manure without adding the byproduct was used for control treatment, and swine manure mixed with either corn silage or kitchen waste was used for other treatments. Volume of biomethane ($CH_4$) generated from digested materials was quantified before and after byproduct treatments. In result, a 1.4-fold higher biomethane, about 0.556 L/$L{\cdot}d$, was produced when the content of TS was controlled at 10 %, compared at 5 %, about 0.389 L/$L{\cdot}d$. When the swine manure was mixed with the corn silage or kitchen waste, a two-fold higher biomethane was produced, about 1.theand 1.0heL/$L{\cdot}d$, respectively, compared to the control treatment. Biogas production from organic dry matter (odm) was a3, 362eand 2h6 L/kg odm${\cdot}$d for control, corn silage, and kitchen waste treatment, respectively. The lower biogas production in the treatment of kitchen waste than that of corn silage is associated with its relatively high odm contents. The methane concentration during the whole process ranged from 40 at the beginning to 70 % at the end of process for both the control and kitchen waste treatments, and ranged from 52 to 70 % for the corn silage treatment. Hydrogen sulfide ($H_2S$) concentration ranged between 350 and 500 ppm. All the integrated results indicate that addition of organic byproduct into animal manure can double the generation of biogas from anaerobic fermentation process.

• Korean Journal of Soil Science and Fertilizer
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• v.4 no.1
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• pp.101-107
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• 1971

1. 수도(水稻)의 생육기간(生育其間)만이라도 심수하(甚水下)에 있어야하는 논은 투수성(透水性)의 영향(影響)을 받아야 하고, 동영향(同影響)은 수도생육(水稻生育)과 관련(關聯)을 갖는다. 즉(卽) 투수성(透水性)이 클 경우(境遇)에는 물의 소비(消費)가 많을 뿐만이 아니라 영양분(營養分)의 유실(流失) 및 유효화(有效化)가 억제(抑制)되고, 투수성(透水性)이 적을 경우(境遇)에에는 심(甚)한 환원상태(還元狀態)의 발달(發達)로 유해성분(有害成分)이 집적(集積)되어 수도(水稻)의 생육(生育)은 해(害)를 입게된다. 이렇게 생각하면 투수성(透水性)으로 본 벼 생육(生肉)에 가장 적합(適合)한 토양조건(土壤條件)이 있을 것인바 이 조건(條件)은 토양(土壤)의 종류외(種類外)에도 수도(水稻)의 품종(品種)이나 생육시기(生育時期)에 따라 다른것이다. 2. 투수성(透水性)이 큰 토양(土壤)에는 잘 유거(流去)되지 않는 비료(肥料)(유기물비료(有機物肥料)나 용성연비(熔成憐肥) 등(等))를 쓸것이며, 투수성(透水性)이 적고 유해성분(有害成分)이 집적(集積)되기 쉬운 토양(土壤)에는 류산근(류硫酸根)이나 유기질(有機質) 비료등(肥料等) 유해성분(有害成分)의 집적(集積)을 증가(增加)할 우려가 있는 비료(肥料)의 시용(施用)을 삼가야 한다. 3. 기왕(旣往) 국내(國內)에서 실시(實施)한 시험결과(試驗結果)에 의(依)하면 중점토(重粘土)에서는 연산(憐酸), 중점토(重粘土)와 퇴화염토(退化鹽土)에서는 가리결핍(加里缺乏)을 일으키기 쉬운것 같으나 그 밖의 토양(土壤)에서는 뚜렷한 경향(傾向)이 인정(認定)되지 않는다. 4. 투수성(透水性)이 큰 토양(土壤)에서는 산적토(山赤土)를 객토(客土)하는 경우(境遇)가 많은 바 이때에는 금비(金肥) 특(特)히 질소질비료(窒素質肥料)를, 객토(客土)하지 않았을때 보다 많이 써야 하는것이 일반적(一般的)이다. 5. 투수성(透水性)이 약(弱)하고 유해성분(有害成分)이 집적(集積)되기 쉬운 논에는 우선(于先) 석회(石灰)(소석회(消石灰))를 시용(施用)하여 토양반응(土壤反應)을 높여야 하며 심(甚)한 노후답(老朽畓)이나 퇴화염토(退化鹽土)에는 석회(石灰)와 아울러 규산질(珪酸質) 비료(肥料)를 시용(施用)한 연후(然後)에 비료(肥料) 삼요소(三要素) 시용(施用)을 고려(考慮)해야 할 것이다. 산성(酸性)으로 기울어져있는 많은 우리나라 논 토양(土壤)에는 알칼리성(性) 비료(肥料)인 용성연비(熔成憐肥)를 시용(施用)하는 것이 효과적(效果的) 일것이다. 6. 소석회(消石灰)와 규산(珪酸)의 시용(施用)은 연산(憐酸)과 가리(加里)의 요구량(要求量)을 증가(增加) 할것이며 비료(肥料) 삼요소(三要素)에 대(對)한 수도(水稻)의 응수곡선(應酬曲線)의 모습을 기왕(旣往)의 것과 달리 할것이다.