• Analytical Science and Technology
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• v.30 no.6
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• pp.348-354
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• 2017

In this study, patterns of proteome expression were monitored and specifically expressed proteins in human milk were detected in collected human milk after 1 week, 3 weeks, and 6 weeks from delivery. A quantitative shotgun proteomic approach was used to identify human milk proteins and reveal their relative expression amounts. For each sample, two independent human milk samples from two mothers were pooled, and then three replicated shotgun proteomic analyses were carried out. Casein, which is a highly abundant protein in human milk, was removed, and then trypsin was treated to produce a digested peptide mixture. The peptides were loaded in the home-made reversed-phase C18 fused-silica capillary column, and then the eluted peptides were analyzed by using a linear ion-trap mass spectrometer. The relative quantitation of proteins was performed by the normalized spectral count method. For each sample, 81-109 non-redundant proteins were identified. The identified proteins consisted of glycoproteins, metabolic enzyme, and chaperon enzymes such as lactoferrin, carboxylic ester hydrolase, and clusterin. The comparative analysis for the 63 proteins, which were reproducibly identified in all three replications, revealed that 25 proteins were statically significant differentially expressed. Among the differentially expressed proteins, Ig lambda-7 chain C region and tenascin drastically decreased with the delivery time.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.2
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• pp.113-118
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• 2005

This experiment was conducted to compare the dry matter yields and fled values of winter forage crops using too cultivars of Barley, Rye md Italia ryegrass at the Dae-Ho reclaimed tideland, Korea from 2002 to 2004 2rowing season. Soil salt contents of three forage crops showed highest at sowing time, respectively and after wintering continuously lowed till harvest time. The dry matter yield was 6,668.8, 4,455.6 md 2,591.2 kg / ha, respectively, for Italian ryegrass, Barley and Rye. The highest Crude protein(CP) content was recorded in Italian ryegrass. Acid detergent fiber(ADF) contents were lowed in row with Barley, Italian ryegrass and Rye. Sodium contents in plant tissue were recorded high in line with Barley, Italian ryegrass and Rye. results mentions above suggest Italian ryegrass is suitable winter forage crops for cultivation on reclaimed tideland in view of the good emergence, forage production and its feed value.

• KSBB Journal
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• v.20 no.2 s.91
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• pp.106-111
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• 2005

The functional relationship between the number of mole of an i-fatty acid (Si) included in fish oil and the hydrolysis time(t) was expressed as a mathematical model, $S_i=-{\alpha_i}1n(t)+\beta_i$. The average errors of calculated values on the basis of the measured values were distributed in the range of less than $5\%$ for all the 15 fatty aids composing of fish oil. The equation of hydrolysis rate of each fatty acid was deduced as $v_i={\gamma_i}exp(\frac{S_i}{\alpha_i})$ from the above-mentioned $S_i=-{\alpha_i}ln(t)+{\beta_i}$. Therefore the hydrolysis yields of fatty acids were analyzed using the equation of $S_i\;Vs.\;t.$. The 15 fatty acids were categorized into 4groups from the view point of hydrolysis yield. The hydrolysis yields of the first group, including C14:0, C16:0, C16:1, C18:0, C18:1 (n-7) and 1l8:1 (n-9), were higher than $70\%$ at 48 hr of hydrolysis. Those of the second group, C20:1, C22:1, C18:3, C20:4 and C20:5, were distributed from $40\%,\;to\;60\%$, and third group were around $30\%$. The final group containing only C22:6 was very hard to be hydrolyzed and the yield was less than $20\%$ at the same time.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.417-418
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• 2018

미국 국립가뭄경감센터 (National Drought Mitigation Center, NDMC)는 다양한 가뭄지수를 통합하여 미국 전역의 가뭄진행상황을 모니터링하고 가뭄대응정책 수립을 위한 주요 의사결정정보로 활용하고 있다. 대표적으로 1999년에 개발되어 현재까지 운영 중인 미국가뭄모니터 (United States Drought Monitor, USDM)는 미국 전역에 대하여 가뭄단계를 표시한 지도 (U.S. Drought Monitor map)를 매주 생성하여 제공하고 있다 (http://droughtmonitor.unl.edu/). 가뭄지표(drought index)는 가뭄의 현황과 시공간적인 전개 과정을 분석하고 정량적 가뭄심도 평가 및 가뭄대응계획 수립을 위한 도구로써 다양하게 개발되어 활용되고 있다. 가뭄의 정도를 정량화하기 위하여 개발된 다수의 가뭄지수는 대상과 평가방법에 따라 가뭄을 표현하는 특성이 서로 다르다. 하나의 가뭄지수로는 가뭄특성을 온전히 표현하기 어렵기 때문에, 최근에는 단일 가뭄지수에 의존하기 보다는 다수의 가뭄지수를 이용하되, 여러 가뭄지수 간의 특징을 고려하여 각 가뭄지수가 갖는 장단점을 상호 보완하여 사용하기를 권고하고 있다. USDM은 파머가뭄심도지수 (Palmer Drought Severity Index, PDSI), Soil Moisture Model (NOAA Climate Prediction Center, CPC), 미 지리조사국의 하천유량 주간보고 (USGS Weekly Streamflow), 표준강수지수 (Standardized Precipitation Index, SPI) 등의 주요 가뭄판단지표를 선정하고, 가뭄판단의 기준으로써 각 가뭄지수의 가뭄심도 (drought severity) 및 백분위수 (percentiles)로 등급을 구분하였다. 가뭄등급은 '정상 상태 (none)'를 포함하여 '비정상적인 건조 (abnormally dry, D0)'에서 최악의 가뭄상태를 의미하는 '이례적인 가뭄상태 (exceptional, D4)'에 이르는 6 단계로 구분하고, 정상상태를 제외한 5 단계의 통합가뭄단계로 표시한다. 우리나라에서는 기상청, 수자원공사, 농어촌공사에서 기상/수문/농업관련 가뭄지수의 위험지도를 실시간으로 제공하고 있으며, 각 지표별로 상이한 기준으로 가뭄을 판단하고 있다. 각각의 가뭄지표에 대한 가뭄판단기준은 해당 국가의 장기적으로 축적된 자료를 활용하여 가뭄단계 및 가뭄판단기준의 재설정에 대한 연구가 필요하다. 본 연구에서는 SPI, SC-PDSI, 표준강수증발산지수 (Standardized Precipitation Evapotranspiration Index, SPEI), Evaporative Demand Drought Index (EDDI), 유효가뭄지수 (Effectvie Drought Index, EDI)의 다양한 가뭄지수를 활용하여 USDM의 가뭄심도 및 가뭄판단기준을 적용하고자한다.

• Journal of the Institute of Convergence Signal Processing
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• v.6 no.2
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• pp.95-99
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• 2005

This paper presents an accurate recovery method of the reference clock which is needed for network synchronization in two-way satellite multimedia systems compliant with DVB-RCS specification and which use closed loop method for burst synchronization. In these systems, the remote station transmits TDMA burst via return link. For burst synchronization, it obtains reference clock from program clock reference (PCR) defined by MPEG-2 system specification. The PCR is generated periodically at the hub system by sampling system clock which runs at 27MHz $\pm$ 30ppm. Since the reference clock is recovered by means of digital PLL(DPLL) using imprecise PCR values due to variable random jitter, the recovered clock frequency of remote station doesn't exactly match reference clock of hub station. We propose a robust recovery method of reference clock against random delay jitter The simulation results show that the recovery error is remarkably decreased from 5 clocks to 1 clock of 27MHz relative to the general DPLL recovery method.

• Korean Journal of Microbiology
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• v.34 no.1_2
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• pp.31-36
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• 1998

A broad spectrum of antibiotic action was studied with Centipedin purified from centipede Scolopendra subspinipes multilans L. Koch aganist gram-positive, gram-negative bacteria and fungi. The minimal inhibitory concentrations(MICs) were determined in liquid medium. The significant antibiotic activity was obtained aganist gram-negative Klebsiella pneumoniae ATCC 8308 responsible for causing infection at lung and intestine. The MIC value against Klebsiella pneumoniae ATCC 8308 was $2{\mu}g/ml$, and this Centipedin was active against Proteus vulgaris NRRL B-123. In addition, it has been shown that Centipedin blocks procaryotic RNA transcription and a little of DNA replication system in vitro. Centipedin did not exhibit any significant cytotoxicity against animal cells such as human blood leukemia (HL-60) and mouse B lymphocyte myeloma cell.

• Journal of the Korean Chemical Society
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• v.44 no.4
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• pp.305-310
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• 2000

Adsorpdon behaviors ofNi(II), Co(II), Sr(II), and(I) ions were nvestigated on amorphous alumina with varying pH at three different etalion concentrations under the condition of 0.I M ionlc strength.With increasingmctalion concentration, the percentage of adsorption decreased and the adsorption edge was shifted to a higher pH value. The adsorption data obtained usingthe metal ion concentration of $1.0{\times}10^{-4}$M were quantitatively analyzed by surfacecomplexation modelingof Ni(II) and Co(II) ions adsorbed at high pH (>8)were found to be in hydrolyzed forms.

• Magazine of the Korea Concrete Institute
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• v.11 no.2
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• pp.167-175
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• 1999

Many composite girder bridges have been constructed for about thirty five years. Nowadays they are aged or deteriorated because of the increase in traffic and vehicle loads. In this study, the effect of strengthening with glass fiber sheet is investigated to estimate the possibility for applying to damaged prestressed concrete bridges. One normal and eight cracked specimens which had been preloaded were tested. The cracked specimens were strengthened with either external prestressing or bonding glass fiber sheet, or using both methods. The results showed that the maximum loads are almost same for both methods. So it seems that the strengthening with glass fiber sheet can be used for strengthening damaged prestressed concrete girders. It is important that proper devices should be selected to prevent glass fiber sheet from premature bonding failure below its maximum load, which is similar to end anchorage problem in external prestressing method. It is proved that the devices proposed in this paper have sufficient anchoring capability to increase load carrying capacity.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.3 no.1
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• pp.1-7
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• 2005

Anion exchange chromatography and HDEHP extraction chromatography using DTPA-lactic acid as an eluent were applied in series for the separation of $^{241}$Am and $^{244}$Cm in radwaste samples. The separated elements were determined by electrodeposition at the sodium hydrogen sulfate-sodium sulfate buffer solution followed by alpha-spectrometry. The recovery yields of $^{241}$Am and $^{244}$Cm were 85.2$\pm$$15.3\%$, respectively, from the synthetic solution of spent nuclear fuel sample. The amounts of 241Am and 2440m determined in radwaste sample solutions of condensate bottoms were at the range of 1.5-1.9 Bq/g and -1.7 Bq/g, respectively.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.3 no.1
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• pp.17-21
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• 2005

Shielded laser ablation system composed of laser system, image analyser, XYZ translator with motion controller, ablation chamber, manipulator and various optics was designed. Nd:YAG laser which can be tunable from 1064 nm to 266 m was selected as light source. CCD camera(< $\pm$200) was chosen to analyze a crater less than 50 un in diameter. XYZ translator was composed of three linear stage which can travel 50 w with a minimum movement of 1 um and motion controller. Before the performance test, each part of system was optically aligned. To perform the ablation test, the specimen was ablated by 50 um interval and observed by image analyser The shape of crater was almost round, indicating laser beam has homogeneous energy distribution. The resolution and magnification of image system were compatible with the design.