• Korean Journal of Medicinal Crop Science
• /
• v.13 no.4
• /
• pp.133-137
• /
• 2005

Experiments were conducted to find the optimal acclimatization conditions for Eleutherococcus senticosus plantlets regenerated from bioreactor cultured somatic embryos, various acclimatizing conditions were compared regarding both survival rate and growth of the plantlets. Among the various temperature and artificial soil tested, the highest survival rate (88%) was observed when plantlets were acclimatized in Klasmann bed soil at $10^{\circ}C$ When in vitro plantlets directly transplanted to field environment, shading treatment was necessary and 50% shading was more effective than 30% shading. Transplanting season were also important for successful acclimatization of in vitro cultured plantlets, transplanted on March 20 with 50% shading exhibited the best survival rate and further growth.

• Journal of Plant Biotechnology
• /
• v.31 no.2
• /
• pp.121-126
• /
• 2004

Bleeding heart (Dicentra spectabilis L. Lemaire) is one of the most valuable wild flower in Korea. This work was conducted for the mass production of somatic embryos through suspension culture and more effective plant regeneration system in Dicentra spectabilis. High-frequency embryogenic callus proliferation was achieved in SH liquid medium supplemented with 1 mg/L 2,4-D. Half-strength SH medium was suitable concentration for somatic embryo induction and germination. About 5,000 embryos were produced per 250$m\ell$ flask after 4 weeks of culture. Germination rate of somatic embryos was decreased when GA$_3$ was added in medium. The plantlets showed a 58％ survival rate when transferred to pots after 1 month of culture. The results indicate that micropropagation procedure via somatic embryogenesis can be applied for an efficient mass propagation of Dicentra spectabilis.

• Journal of Bio-Environment Control
• /
• v.20 no.3
• /
• pp.197-203
• /
• 2011

This experiment was carried out to investigate the growth characteristics of virus-free sweetpotato in hydroponic bag culture. The rooted one-node and upper-shoot cuttings of 'Yeonhwangmi' and 'Mannamiwere' transplanted into the plastic bag (0.5 mm of thickness, $W300{\times}L1,200{\times}H120mm$) culture system filled with commercial horticultural media (TKS-2, Flora Gard Co.) and cocopeat + perlite (3 : 7, 5 : 5, 7 : 3, (v/v). Nutrient solution of National Horticultural Research Station in Japan was separately irrigated $3times{\times}30minutes$ per day (10:00, 14:00 and 20:00 with timer control) by the drip irrigation. The growth of two varieties was not significantly different among four substrates in 15 days, but it was excellent in commercial horticultural media (TKS-2) and cocopeat 3 : perlite 7 (v/v) in 20 days. The growth in 'Yeonhwangmiwas' promoted than that in 'Mannami', and the upper-shoot cuttings of 'Yeonhwangmi' and 'Mannami' showed a significant vine elongation (over 10 and 2 cm, respectively) compared to the one-node cuttings. Fresh weight and dry weight in cocopeat 3 : perlite 7 (v/v) were significant increased than that in cocopeat 7 : perlite 3 (v/v). Therefore, the bag culture system filed with cocopeat 3 : perlite 7 (v/v) was successfully applied on propagation of virus-free sweetpotato seedling, and the result expected that the bag culture system could be commercialized without high financial costs for farmers.

• Journal of agriculture & life science
• /
• v.46 no.2
• /
• pp.35-42
• /
• 2012

This study was conducted to investigate growth responses at 45 days after cutting in six Sedum cultivars (S. reflexum 'Blue Spruce', S. rupestre 'Angelina', S. sexangulare, S. sexangulare var. elatum, S. spurium 'Atropurpureus', S. takesimense) using vegetation mats (individual coir tape and jute treated with different thicknesses and soil compositions) to broaden usefulness of evergreen Sedum cultivars. All the treatments including both single and interaction between treatments showed significant effects (p<0.001) on the number of branches, plant height, and shoot fresh weight except the number of branches treated with different mat types. No. of branches was showed two times in compost than in sand regardless of cultivars and mat types with the exception of S. takesimense showing no added branches in all treatments. Each plant height of all the cultivars except 'Angelina' showed the highest value under the conditions treated with 5 mm thick coir tape using compost. The fresh weight of 'Blue Spruce' treated with 5 mm thick coir tape using compost was 13.19 g of which value was the highest in all treatments. The better growth response in all Sedum cultivars was shown in the treatment of 5 mm thick coir tape than with jute net treatment.

• Korean Journal of Plant Resources
• /
• v.21 no.2
• /
• pp.111-116
• /
• 2008

This study was carried out to induce plant regeneration via multiple shoot formation from sucker explants of Rubus fruticosus L. ${\times}$ R. parvifolius L. To induce adventitious shoots, the sucker explants were sterilized in 1.2% sodium hypochlorite (NaOCl) solution, and then were cultured on the full and 1/2 MS solid medium supplemented with BA (0.1, 0.5, 1.0, $2.0mg{\cdot}L^{-1}$). After 4 weeks of culture, the highest frequency (83.3%) of shoot formation from sucker explants was obtained from the full MS medium with $1.0mg{\cdot}L^{-1}$ BA. The highest shoot number (3.7) per explant was obtained from the full MS medium with $0.5mg{\cdot}L^{-1}$ BA. After 12 weeks of culture, the number of shoots (15.4) per explant was increased. The highest frequency (95%) of root formation was obtained from the 1/2 MS medium, when the explant with shoot were cultured on the full, 1/2 and 1/4 MS medium. The survival rate of the plantlets after transfer to plastic pots containing sand, soil, and vermiculite (1.1:1, vol.) was 95%. The results indicate that multiple shoot procedure can be applied for an efficient mass propagation of Rubus fruticosus L. ${\times}$ R. parvifolius L.

• Journal of Plant Biotechnology
• /
• v.34 no.1
• /
• pp.1-6
• /
• 2007

Effect of BA concentrations and culture methods on in vitro plant multiplication from shoot-tip cultures of Wasabia japonica was studied. Shoot-tips with leaf primordia and apical meristem were cultured on MS basal medium for all the experiments. Liquid medium for 2 weeks followed by semi-solid medium for 4 weeks containing 1.0 mg/L BA was the best to number of shoots (22.8) and shoot length (3.5 cm). Shoots proliferated could be divided into ca. 5 to 11 of cultures for the multiplication of plantlets. Divided plantlets showed root formation (90%) well onto MS basal medium without growth regulators like IBA and NAA. After rooting, all the plantlets transferred into the pots containing composed soil (bio-media Co., peatmoss $8{\sim}10%$, coir dust $66{\sim}70%$, zeolite $13{\sim}17%$, vermiculite $3{\sim}7%$, perlite $2{\sim}4%$) and grown well into whole plants with multiple shoots.

• Korean journal of applied entomology
• /
• v.44 no.2
• /
• pp.125-130
• /
• 2005

The ecological characteristics of the specialist insect predator, Oligota kashmirica benefica, were investigated for developing artificial rearing method with special interest on the oviposition behavior, the pattern of adult emergence and temperature in storage, and its effect on the density of the citrus red mite, Panonychus citri. Female beetle oviposited eggs mainly, $95\%$ of the eggs, on the bottom of leaves of the yuzu tree, and $91.3\%$ of them were covered with ecdysis skin of the red mites or the feces of themselves. The rate of adult emergence of the beetle rose up to $86.7\%$ when the horticultural media was provided for pupation from $60\%$ when it pupated in upland soil. Most larvae, $88\%$ of them, were found from the surface to the depth of 2 cm in the horticultural media. The optimum temperature for the storage of the adult beetle was found to be $12^{\circ}C$, at this temperature the 96.7, 73.3 and $70.0\%$ of the beetle survived for 10, 20, and 30 days, respectively. The control effect of the citrus red mite was appeared highest by releasing the beetle at the rate of one beetle against 150 mites.

• Journal of Korean Society of Forest Science
• /
• v.103 no.3
• /
• pp.359-367
• /
• 2014

This study was carried out to determine the effects of seed storage methods and pre-treatments on seed germination, soil types and shading conditions on potted seedlings growth of S. sieboldiana var. pendula and S. sieboldiana for. xanthocarpa, endemic species in Korea. The seed germination rate of S. sieboldiana var. pendula was the highest at 48.6% when seeds were treated with stratification for 60 days and then soaked in 100 ppm $GA_3$. And seed germination rate of S. sieboldiana for. xanthocarpa was the highest at 51.4% when seeds were pre-chilled for 30 days and then soaked in 100 ppm $GA_3$. And potted seedlings of S. sieboldiana var. pendula showed the best quality under 50% shading in bedsoil with the growth characteristics of plant height (41 cm), number of leaves(8), leaf width (16 cm), leaf length (18 cm), root length (22 cm), fresh weight(aerial part/root part; 20/6.9 g) and dry weight(aerial part/root part; 4.5/2.0 g). And potted seedlings of S. sieboldiana for. xanthocarpa showed the best quality under non-shading in bedsoil with the growth characteristics of plant height (39 cm), number of leaves(10), leaf width (12 cm), leaf length (15 cm), root length (22 cm), fresh weight (aerial part/root part; 21/13 g) and dry weight (aerial part/root part; 4.2/2.2 g). Therefore, seeds treated with stratification or prechilling and then soaked in $GA_3$ were effective in germination of S. sieboldiana var. pendula and S. sieboldiana for. xanthocarpa, and potted seedlings should be cultivated in bedsoil under non or 50% shading condition.

• Journal of Bio-Environment Control
• /
• v.26 no.4
• /
• pp.424-431
• /
• 2017

This study was conducted to set the optimum nutrient solution concentration by growth stage for new strawberry cultivars 'Berrystar' and 'Jukhyang'(Fragaria ${\times}$ ananassa Duch. cvs. 'Berrystar', 'Jukhyang') grown through hydroponics to improve the quality and yield. Three different EC levels were applied to the nutrient solution. The treatment levels were 0.7, 1.0 and 1.3 times higher than the nutrient concentration standard for 'Seolhyang' based on the 'Manual for strawberry cultivation' of Rural Development Administration. Based on the results, there were no significant differences in growth of 'Berrystar' by EC level. 'Jukhyang' showed the most vigorous growth grown in 1.3 times higher nutrient concentration. While the growth of 'Berrystar' and 'Jukhyang' grown in higher EC level has leaves with more chlorophyll concentration. However the quantum yield of leaves was not affected by the treatments. On the treatment with 1.3 times higher EC level, the weight, length, width and firmness of 'Berrystar' and 'Jukhyang' were significantly high. The sugar contents of the harvest analyzed by HPLC did not differed particularly, but the percentage composition of reducing sugar and non-reducing sugar were presented differently depending on the treatments. Marketable fruit yield increased as nutrient concentration increases. However, there were no large differences by treatments. Meanwhile, 'Jukhyang' showed significant difference by nutrient concentration and had the largest yield for a treatment grown in 1.3 times higher EC level. Based on these results, it is recommended to provide the same nutrient solution concentration level to the nutrient concentration standard of 'Seolhyang' for 'Berrystar', and the 1.3 times higher level for 'Jukhyang'.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.45 no.5
• /
• pp.42-59
• /
• 2017

To investigate rooting and community forming ability, sod strips of Gramineae such as Miscanthus sacchariflorus (Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg., and Arundinella hirta(Thunb.) Tanaka were planted horizontally at a width of 10~15cm in October 2010 on the bank slope of Kwangju Stream in Korea. Seeds of each species were sown in April 2010 in plastic seedbeds(60cm length ${\times}$ 30cm width ${\times}$ 5cm depth) filled with 4cm of garden soil and grew until October 2010 to form rectangular sod, which then was cut into multiple strips(20cm length ${\times}$ 6cm width ${\times}$ 4cm root depth). Weeds growing on the experiment sites were removed twice a year until those four species had formed a community by 2013. Weeds were not eradicated during 2014 and 2015 to observe whether the community formation can be maintained without weed removal. t-tests on stem numbers and heights in May, July, and September were conducted between 2013 and 2014, and between 2014 and 2015. Stem numbers and heights of the four species in 2014 were significantly decreased(p<0.001) compared with those in 2013 due to weed damage to their growth in 2014. Stem numbers and heights of Miscanthus sacchariflorus(Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg. in 2015 were similar to those in 2014(p>0.05). Stem numbers and heights of Arundinella hirta(Thunb.) Tanaka in 2015, however, were significantly reduced compared with those in 2014(p<0.001) due to weed impact on its growth. After three years of weed removal, a community of each species was formed. Miscanthus sacchariflorus(Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg. were able to establish communities without any further weed removal. Arundinella hirta(Thunb.) Tanaka, however, was not able to maintain its community due to considerable damage to its growth caused by weeds even after three years of weed removal. Miscanthus sacchariflorus(Maxim.) Benth., Miscanthus sinensis var. purpurascens(Andersson) Rendle, Imperata cylindrica var. koenigii(Retz.) Pilg. are more suitable to sod strip plantings on stream bank slopes in terms of the maintenance of community formation without weed eradication. t-test on stem numbers and heights each September during the five year experiment period between sod strip planting and potted plants of the four species were conducted. Stem numbers of strip sod plantings were significantly higher(p<0.001) than those of potted one. Heights of strip sod plantings, however, were significantly lower(p<0.05) than those of the potted samples. Therefore, strip sod planting is more advantageous regarding bank slope erosion protection due to the higher number of stems.