• Korean Journal of Food Science and Technology
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• v.41 no.3
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• pp.292-295
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• 2009

To discriminate the geographical origin (Korea vs. China) of soybean (Glycine max(L.) merrill) samples (Korean samples n=25, Chinese samples n=24), proximate composition of soybeans and relaxation times were analyzed using low field NMR. Composition results indicate that there are no significant differences in moisture, fat, or ash contents between soybeans. The crude protein content of Korean soybeans, however, was higher than that of Chinese soybeans (p<0.05). The relaxation times of T1-IR (p<0.0001), T1-SR (p<0.0001), and T2-SE (p<0.0086) in Korean soybeans were longer than those in Chinese soybeans. The geographical origin of soybeans could be identified using a canonical discriminant analysis using two relaxation times (T1-IR and T1-SR) with 96% accuracy. Furthermore, in this study, a canonical discriminant analysis using four relaxation times (T1-IR, T1-SR, T2-SE, and T2-CPMG) could discriminate the geographical origin with 100% accuracy. It was possible to identify the geographical origin of Korean and Chinese soybeans using relaxation times from 10 MHz NMR.

• Korean Journal of Food Science and Technology
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• v.48 no.5
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• pp.418-423
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• 2016

This study was conducted to identify the geographical origin of soybeans in Cheonggukjang by analyzing its organic components and inorganic elements with Fourier transform near-infrared spectroscopy (FT-NIRS) and with energy dispersive X-ray fluorescence (ED-XRF) coupled with multivariate statistical analysis. For method development, 280 samples from various regions were collected and analyzed. The discriminant accuracy for the developed methods was 97.5% for FT-NIRS and 98.0% for ED-XRF with multivariate statistical analysis. A validation test confirmed the discriminant accuracy to be 96.3% for FT-NIRS and 95.0% for ED-XRF. Overall, the results showed that methods using FT-NIRS and ED-XRF could be used to identify the geographical origin of Cheonggukjang.

• Analytical Science and Technology
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• v.14 no.4
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• pp.311-316
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• 2001

We have investigated the trace amount in an oriental medicine in oder to determine the geographical origin by using inductively coupled plasma-atomic emission spectrometry(ICP-AES) and chemometric anlysis with principal component analysis(PCA) and pattern recognition. Astragali Radix from several agricultural places in Korea was selected as an example of the oriental medicine and analyzed by ICP-AES. The dried Astragali Radix sample was treated with $HNO_3$ and $H_2O_2$, then digested using microwave oven. Elements such as Mg, Al, K, Ca, Ti, Mn, Fe, Cu, Zn, and Ba with different concentrations were found an used for the identification of the origin of agriculture places. Especially, the concentration of Al, Fe, Zn and Ti were employed to investigate the relationship between. Astragali Radix and the agricultural places by PCA and pattern recognition. We have made a program that is based on chemometrics in analytical spectroscopy. The results of the chemometrics analysis indicated that a distinction among Yechon and Chechon, Chungson, Kurye and Chinese Astragali Radix could be made. We believe that principal component analysis(PCA) and pattern recognition is a valuable tool to identify the origin of Astragali Radix in terms of the agricultural place.

• Korean Journal of Food Science and Technology
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• v.35 no.5
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• pp.994-997
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• 2003

Domestic and imported Cnidium officinale were investigated using GC based on a SAW sensor. Volatile components from the herb were detected by GC with a Surface Acoustic Wave (SAW sensor without any pretreatment. This system produced a frequency proportional to the amount of column effluent deposited on the SAW sensor. It could discriminate between domestic and imported Cnidium officinales. This was achieved by using a pattern recognition and a visual pattern called a $VaporPrint^{TM}$, derived from the frequency and chromatogram of the GC-SAW sensor. The origins of Cnidium officinale was well discriminated with the direct use of $VaporPrint^{TM}$.

• Korean Journal of Food Science and Technology
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• v.35 no.1
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• pp.144-148
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• 2003

Volatile components were detected from domestic and imported Angelica gigas Nakai without any pretreatment using GC based on Surface Acoustic Wave (SAW) sensor. This system produced a frequency proportional to the amount of column effluent deposited on the SAW sensor. Discrimination between domestic and imported Angelica gigas Nakai was achieved through recognition of visual pattern using $VaporPrint^{TM}$ derived from frequency and chromatogram of GC-SAW sensor.

• Journal of Mushroom
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• v.14 no.4
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• pp.232-236
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• 2016

This study aimed to discriminate the geographical origins of domestic and chinese Wolfiporia extensa. They were subjected to oxygen (45.32 to 48.07%), carbon (38.09 to 40.12%), hydrogen (6.05 to 6.78%), and nitrogen (0.16 to 0.23%). Antioxidant activity was examined by DPPH free radical scavenging activity. According to the results of the experiment, no significant differences were found between domestic ($IC_{50}$, 7.25 mg/ml) and Chinese ($IC_{50}$, 8.35 mg/ml) W. extensa. However, as determined by the inorganic mineral contents were significantly different between domestic and Chinese W. extensa. The amount of potassium in domestic and Chinese W. extensa was and $33.14{\pm}17.27%$, $47.60{\pm}8.78%$, respectively. The results of this study suggested that the analysis of inorganic components by ED-XRF should be useful for origin identification of W. extensa.

• Journal of Life Science
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• v.14 no.4
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• pp.627-635
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• 2004

In order to distinguish the genetic polymorphism among the olive flounder (Paralichthys olivaceus) obtained from East sea of Jumunjin, aquaculture of Tongyoung and Geoje, and East sea of North Korea, the ND-4 and cytochrome b genes of olive flounder were divided into 5 regions. Each region was analyzed by degenerating gel electrophoresis scanning (DGES), and by subsequent DNA sequencing. The DGES disclosed characteristic DNA polymorphisms in ND-4-2 and ND-4-3 regions of olive flounder, which were also confirmed by the DNA sequencing. The olive flounders obtained from the different marine areas showed DNA mutations in ND-4-2 region (G390A, C402T, and A411G； GenBank： AB028664), and also showed frequent DNA mutations in ND-4-3 region (C515G, C537T, C538T, A567G, G714A, C736T, G756A, A759T, T817C, and T829G), white the cytochrome b gene showed no DNA mutation both in the DGES and DNA sequencing. These data suggest that the ND-4-2 and ND-4-3 regions are candidate loci to distinguish the origin of olive flounder, and that the DGES used in this study provided fast and reliable informations for the genetic polymorphism.

• Journal of Life Science
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• v.16 no.1
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• pp.88-94
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• 2006

The random amplified polymorphic DNA (RAPD) technique was investigated as a potential tool for the origin identification of olive flounder (Paralichthys olivaceus) and redlip croaker (Pseudosciaena polyactis). Olive flounder specimens were collected from North Korea and several locations of South Korea (Jumunjin, Tongyoung and Geoje). Fishes obtained from Tongyeong and Geoje were cultured products. Redlip croaker specimens were collected from South Korea and China. Consistent and distinct diagnostic bands were easily identified in the RAPD patterns of the olive flounder specimens. Although consistent diagnostic bands rarely appeared in the RAPD pattern of redlip croaker specimens because of their genetic heterogeneity, we were able to find potential diagnostic bands in the average RAPD pattern of each origin.

• Korean Journal of Food Science and Technology
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• v.34 no.5
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• pp.787-791
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• 2002

Optimal extraction, separation, and capillary rinsing conditions for capillary electrophoresis (CE) were established to identify the cultivation site (domestic vs. foreign) of Job's-tears (Coix lachrymajobi var. mayuen) using 240 samples (domestic sample n = 121, foreign sample n = 119). Job's-tears was extracted with 30% ethanol and separated on a $50-{\mu}m-I.D.$ untreated fused-silica capillary. Optimal analytic conditions were: temperature, $45^{\circ}C$; voltage, 15 kV; detector rise time, 0.1 sec; and pressure injection, 20 sec. Separation of peak investigated using 0.1 M phosphate buffer (pH 2.5) containing 0.05% hydroxypropylmethylcellulose (P buffer) revealed the optimal separation buffer was P buffer containing 26 mM hexane sulfonic acid with 30% methanol. Under the optimal conditions established for CE, the average correct identification percentage of domestic or foreign Job's-tears was 82%.

• Korean Journal of Food Science and Technology
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• v.49 no.4
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• pp.349-354
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• 2017

The geographical origins of raw ginseng (RG) were discriminated using an electronic tongue. Taste screening, DFA (discriminant function analysis), and CDA (canonical discriminant analysis) were used to statistically analyze the data. The taste profile patterns of umami, bitterness, and sweetness of the Korean RG was different from those of the Chinese RG. The Korean RG was stronger than the Chinese RG regarding the taste of umami. DFA discriminated the geographical origins of 154 samples, with a few overlapping samples, between the Korean and Chinese RG. CDA showed that the accuracy of origin discrimination for the Korean and Chinese RGs were 87.01 and 94.81%, respectively. The final accuracy of origin discrimination was 90.91%. The distance between the centroids of each group was 2.7463. Thus, the electronic tongue analysis can be used to efficiently differentiate the geographical origins of RG.