• Title/Summary/Keyword: 우세장

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Metagenomic Analysis of Jang Using Next-generation Sequencing: A ComparativeMicrobial Study of Korean Traditional Fermented Soybean Foods (차세대 염기서열 분석을 활용한 장류의 메타지놈 분석 : 한국 전통 콩 발효식품에 대한 미생물 비교 연구)

  • Ranhee Lee;Gwangsu Ha;Ho Jin Jeong;Do-Youn Jeong;Hee-Jong Yang
    • Journal of Life Science
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    • v.34 no.4
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    • pp.254-263
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    • 2024
  • Korean jang is a food made using fermented soybeans, and the typical products include gochujang (GO), doenjang (DO), cheonggukjang (CH), and ganjang (GA). In this study, 16S rRNA metagenome analysis was performed on a total of 200 types of GO, DO, CH, and GA using next-generation sequencing to analyze the microbial community of fermented soybean foods and compare taxonomic (biomarker) differences. Alpha diversity analysis showed that in the CHAO index, the species richness index tended to be significantly higher compared to the DO and GA groups (p<0.001). The results of the microbial distribution analysis of the GO, DO, CH, and GA products showed that at the order level, Bacillales was the most abundant in the GO, DO, and CH groups, but Lactobacillales was most abundant in the GA group. Linear discriminant analysis effect (LEfSe) analysis was used to identify biomarkers at the family and species levels. Leuconostocaceae, Thermoactinomycetaceae, Bacillaceae, and Enterococcaceae appeared as biomarkers at the family level, and Bacillus subtilis, Kroppenstedtia sanguinis, Bacillus licheniformis, and Tetragenococcus halophilus appeared at the species level. Permutational multivariate analysis of variance (PERMANOVA) analysis showed that there was a significant difference in the microbial community structure of the GO, DO, CH, and GA groups (p=0.001), and the microbial community structure of the GA group showed the greatest difference. This study clarified the correlation between the characteristics of Korean fermented foods and microbial community distribution, enhancing knowledge of microorganisms participating in the fermentation process. These results could be leveraged to improve the quality of fermented soybean foods.

Characterization of Microstructures and Fracture Toughness of SR Specimen in Granitic Rocks (화강암에서 SR 시편의 파괴인성과 미세구조적인 특징)

  • Lee, Sang-Eun
    • Tunnel and Underground Space
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    • v.20 no.3
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    • pp.217-224
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    • 2010
  • Three relatively homogeneous granitic rocks were studied to investigate the relationship between their microstructural properties and fracture toughness. Fracture toughness and ultrasonic velocity were varied with the orientation of mineral's long axis and microcrack, obtained from optical microscope. The lowest fracture toughness values are obtained, when the fracture propagates parallel to weakness planes which have the orientation of mineral's long axis and microcrack, in other words, when weakness planes develop perpendicular to the direction of tensile stress agrees with that of rift plane. The fracture toughness values, measured with the short rod method, varied from 1.63 to 2.62 MPa $m^{0.5}$, and their values are related with the average grain size and average microcrack length.

Sexual Dimorphism in a River Sculpin(Cottus poecilopus Heckel) in Korea (한국산 둑중개 (Cottus poecilopus Heckel)의 성적 이형)

  • Byeon, Hwa-Kun
    • Korean Journal of Ichthyology
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    • v.8 no.1
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    • pp.14-21
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    • 1996
  • The sexual dimorphism of river sculpin (Cottus poecilopus Heckel) was investigated samples were collected from the Chiak stream from January 1993 to April 1994. Sexual dimorphism in body size and some morphological characteristics were observed in this species ; males were larger in size and had a larger mouth, anal fin, ventral fin and pevic fin than females. Males have a genital papilla and nuptial color, but females do not. Females may mate preferentially with large males. Larger males could be superior in nest defense and fanning of eggs to smaller ones, thus resulting in higher survival of eggs. Similary, the larger mouth in males could be advantageous in acquiring and defending their nests. Such sexual dimorphism in this species may reflect to outcome of sexual selection.

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A Case Study on the Vibration Characteristics of Tunnel Blasting in Igneous Rock (화성암반에서 터널발파 진동측정치의 분석에 관한 사례 연구)

  • 윤성현;안명석;이광열
    • Explosives and Blasting
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    • v.21 no.1
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    • pp.69-76
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    • 2003
  • Test blasting has been performed with V-cut to investigate the characteristics. Blasting vibrations were measured at two directions, the proceed direction and side direction. Propagation characteristics were determined by regression analysis; square root scaled distance and cube root scaled distance with maximum charge per delay of the blast. Testing result, The cross point was 62m in the allowable vibration velocity of 3mm/sec and 46m In 5mm/sec. Also, vibration level with measuring point was highest and decayed fastest, adapting to cube root scaled distance, for the proceed direction on ground.

반응성 스퍼터링 후 열처리를 이용한 CIGS 박막의 조성비 변화에 따른 특성분석

  • Lee, Ho-Seop;Park, Rae-Man;Jang, Ho-Jeong;Kim, Je-Ha
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.08a
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    • pp.375-375
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    • 2011
  • Cu(In1-xGax)Se2 (CIGS)박막증착법 중 금속 전구체의 셀렌화 공정법은 다른 제조 방법에 비해 대면적 생산에 유리하고, 비교적 공정 과정이 간단하다는 장점이 있다. 이 제조 방법은 금속 전구체를 만든 후에 셀렌화 공정을 하게 된다. 셀렌화 공정은 대부분 H2Se 가스를 사용하지만 유독성으로 사용하는데 주의해야 한다. 본 실험은 H2Se를 사용하지 않고 Se원료를 주입하기 위해 Se cracker를 사용했고 금속 전구체 증착과 셀렌화를 동시에 하는 반응성 스퍼터링 후 열처리 법을 이용하여 CIGS 박막을 증착 했다. CIGS의 박막의 Cu/[In+Ga], Ga/[In+Ga]비를 변화시켜 특성변화를 관찰했다. Cu/[In+Ga]비가 감소할수록 CIGS의 결정방향인 (112) 이 우세하게 발달했고 Ga/[In+Ga]비가 증가할수록 CIGS의 결정면 사이의 값이 작아지기 때문에 CIGS peak의 2-Theta 값이 증가하게 된다. CIGS 박막 태양전지의 구조는 Al/Ni/ITO/i-ZnO/CdS/CIGS/Mo/glass 제작했다. CIGS박막의 조성비가 Cu/[In+Ga]=0.84, Ga/[In+Ga]=0.24인 박막태양전지에서 개방전압 0.48 V, 단락전류밀도 33.54 mA/cm2, 충실도 54.20% 그리고 변환효율 8.63%를 얻었다.

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Reconstruction of April-August Precipitation in Mt. Sorak Region from Tree Rings (나무나이테를 이용한 설악산 지역의 4월~8월 총강수량 복원)

  • ;;Liu Yu
    • The Korean Journal of Quaternary Research
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    • v.15 no.1
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    • pp.47-52
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    • 2001
  • April∼August precipitation (141 years : A.D. 1858∼1998) of Mt. Sorak region (East-central Korea) was reconstructed using a tree-ring chronology of Pinus densiflora sampled from Beakdam Shelter area. During the reconstructed Periods, dry periods were 1880∼1887, 1893∼1901 and 1922∼1938, and wet one 1906∼1918. In the long-term variation, the late 19th and early 20th century were drier than the late 20th century. Major wet/dry periodicities in April∼August precipitation series reconstructed were 3.16 and 4.14 years, indicating that short-term variation were more prominent than long-term one.

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A Survey of the flying Aphid Population at the Alpine Area, Cholla-Pukto (전북고랭지역의 진딧물 밀도조사)

  • Yoon Soon Ki;Choi Seong Sik
    • Korean journal of applied entomology
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    • v.13 no.4 s.21
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    • pp.205-208
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    • 1974
  • This survey was carried out in order to find out population density of aphid at the alpine area. Thetraps were set from May 1 to October 31 in 1973. The summarized results are as follows; 1. About 37 species of aphids were trapped, including 4 species of potatao virus vectors. 2. Of these, dominant species are Aphis gossypii Koch, Aphis lerodendri Matsumura, and Lipahis erysimi Kaltenbaeh. The $67\%$ of 3, dominant species consisted of the trapped total aphids. 3. The potato virus vectors are Myzus persicae Sulzer, Aulacorhum solani Kaltenbach, Lipaphis erysimi Kaltenbaeh and Aphis gossypii Glover. 4. Tile number of aphids and vectors at the alpine area is considerably lower than that at the level land. 5. The peak of the flying aphid occurrence is shown in the latter part of September.

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Preliminary Result of Lineament Analysis for the Potential Site Selection of HLW Geological Disposal (HLW 지층처분 광역 후보부지 선정을 위한 선형구조 예비 분석 결과)

  • Ko, Kyoungtae;Kihm, You Hong;Lee, Hong-Jin
    • Economic and Environmental Geology
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    • v.51 no.2
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    • pp.167-176
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    • 2018
  • It is necessary to consider various geological parameters such as lithology, geological structure, earthquake, hydraulic geology, geochemistry, geological engineering, and geothermal in order to select potential sites for HLW(high-level radioactive waste) geological disposal. In particular, the geological lineament reflects the characteristics of various geological parameters and can be used as an important criterion for site selecting such as nuclear power plants and HLW repositories. In this paper, the Finnish lineament classification method for HLW disposal site selection through the lineament analysis was applied to the lineament data in the Korean peninsula. For this purpose, we used previous lineament data from the KIGAM(Korea Institute of Geoscience and Mineral Resources) and obtained new lineament data from the field geologists such as structural geologist, paleoseismologist, and geomorphologist. To ensure the reliability of the new lineament analysis data, we used high-resolution satellite images and hill-shade relief maps which were constructed by a digital elevation model. In the prevailing direction analysis from the acquired lineament data, the NNE-SSW direction was the most dominant, but the ENE-WSW and NNW-SSE directions also showed highly frequency depending on the experts. Applying the Finnish classification method, the geometrical development characteristics of the lineament corresponding to the Class 1 and 2 used for the wide-wide candidate site were compared. As a result of direction analysis for Class 1, the NNE-SSW direction was the most dominant and the WNW-ESE direction also showed a high frequency. In the case of Class 2, the NNE-SSW is the most prevalent and WNW-ESE or ENE-WSW direction also had highly frequency depending on the experts. Different lineament analysis results based on the same data are interpreted as a result of subjective experience and analytical criteria from the every experts. Therefore, it is necessary to establish integrated criteria and consider geophysical data for the publication of reliable nation-wide lineament map.

Comparative molecular field analyses (CoMFA) on the antifungal activity against phytophthora blight fungus of 3-phenylisoxazole and 3-phenyl-2,5-dihydroisoxazol-5-one derivatives (고추 역병균에 대한 3-phenylisoxazole과 3-phenyl-2,5-dihydroisoxazol-5-one 유도체들의 살균 활성에 관한 비교 분자장 분석 (CoMFA))

  • Sung, Nack-Do;Lee, Hee-Chul
    • The Korean Journal of Pesticide Science
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    • v.6 no.2
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    • pp.72-79
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    • 2002
  • 3D-QSAR between fungicidal activitives ($pI_{50}$) against metalaxyl-sensitive (SPC: 95CC7105) or metalaxyl-resisitant (RPC: 95CC7303) isolate of phytophthora blight fungus (Phytophthora capsici), and a set of 3-phenylisoxazole (A) and 3-phenyl-2,5-dihydroisoxazole (B) derivatives as substrates were conducted using comparative molecular field analyses (CoMFA). The antifungal activities of (A) were generally higher than those of (B). And it is assumed that the most stable conformation of the active substrate was approximately planar from conformational search. The CoMFA models proved a good predictive ability and suggested that the electronic field of substrates were higher than hydropohobic field and steric field requirements for recognition forces of the receptor site. And the factors were strongly correlated (cross-validated $q^2>0.570$ & conventional $r^2>0.968$) with the fungicidal activitives. According to the CoMFA analyses, the selectivity factors for RPC suggested that the sterically bulky groups (C14 & C15) and electron withdrawing groups (C15 & C16) have to be introduced to the ortho, meta and para-position on the benzoyl moiety of substrates.

Comparison of microbial community profiling on traditional fermented soybean products (Deonjang, Gochujang) produced in Jeonbuk, Jeonnam, and Jeju province area (제주·호남권 전통된장과 고추장의 미생물 군집구조의 분석)

  • Cho, Sung Ho;Park, Hae Suk;Jo, Seung Wha;Yim, Eun Jung;Yang, Ho Yeon;Ha, Gwang Su;Kim, Eun Ji;Yang, Seung Jo;Jeong, Do Yeon
    • Korean Journal of Microbiology
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    • v.53 no.1
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    • pp.39-48
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    • 2017
  • In order to evaluate the diversity of microbial population of Korean traditional Deonjang and Gochujang produced in Jeju, Jeonnam, and Jeonbuk province area, microbial communities were analyzed using next generation sequencing. In this result, the dominant bacteria of Deonjang in three area were Bacillus amyloliquefaciens, Tetragenococcus halophilus, and Bacillus was major dominant bacteria in Jeonnam (43.16%) and Jeonbuk (64.54%) area. But in Jeju area, Bacillus was 0.22%, which was significantly different from the other two. Equally, the dominant fungi of Deonjang in 3 area were Candida versatilis. Common fungus in Jeonnam and Jeonbuk area was Candida sp., respectively, 64.22% and 33.68% and Micor sp. was a common fungus in Jeonnam (15.66%) and Jeonbuk area (36.73%). But in Jeju area, Candida sp. and Zygosaccharomyces rouxii were dominant than mold. Bacillus subtilis, Bacillus licheniformis, and B. amyloliquenfaciens were the preminant bacteria in the traditional Gochujang in three regions. But there were no common dominant fungi in the 3 regions. Aspergillus sp. and Rhizopus sp. prevailed in Jeju and Jeonnam region, and Zygosaccharomycess rouxii predominanted in Jeonbuk area. These results suggested that the difference in the samples collected for the study were classified into similar groups according to the characteristics of each sample rather than regional characteristics.