• Proceedings of the KAIS Fall Conference
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• 2002.11a
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• pp.193-196
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• 2002

완전인공심장은 크게 정상류형과 박동류형이 있다. 정상류형 인공심장중 축류형 혈액펌프는 기구가 간단하고 비용적형이기 때문에 소형화가 가능한 장점이 있지만, 가동중 발생하는 난류로 인해 용혈현상이 따른다는 단점이 있다. 이 용혈의 형성과정은 실제와 가까운 모의실험을 하지 않고서는 알 수가 없다. 따라서 본 연구에서는 모의 실험단계를 거치지 않고 유한요소해석에 의한 난류평가를 통하여 용혈지수가 가장 낮은 임펠러의 형상을 연구하였다. 난류해석 결과, vane매수가 적을 경우 상대적으로 용혈지수가 낮게 나타나는 것을 알 수 있었으나 vane매수가 적을 경우에는 일정한 출구유량을 얻기 위해 임펠러의 고속회전 이 필요하며 이에 따른 난류에너지가 발생, 높은 용혈지수가 예상되므로 본 논문에서는 vane매수 4매-6매 중 6000-7000rpm의 회전속도사이의 조건으로 설계된 임펠러의 모델이 적당한 것으로 예측할 수 있었다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.1
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• pp.91-95
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• 1993

To investigate hemolysin from Vibrio vulnificus in terms of protein chemistry and immunochemistvy, the simple method to produce antiserum was developed as follows ; Crude hemolysin from Vibrio vulnificus was mixed with cholesterol-phosphatidylcholine-liposome. Only hemolysin with molecular weight of 50kD was selertively bound to the liposome. Thus, without purification of crude hemolysin, liposome bound hemolysin was used as antigen to produce antiserum by injecting into back muscle of a rabbit. Resultant antiserum reacted only with hemolysin. Hemouysin of Vibrio vulnificus from patients and environment was formed single band in gel diffusion precipitation reaction with antiserum.

• Applied Biological Chemistry
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• v.43 no.3
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• pp.190-195
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• 2000

Tolaasin is a peptide toxin produced by Pseudomonas tolaasii and causes a brown blotch disease forming brown, slightly sunken spots and blotches on the cultivated mushrooms. It is a lipodepsipeptide consisting of 18 amino acids and its molecular mass is 1,985 Da. It forms a pore in plasma membranes, resulting in the disruption of membranes of fungal, bacterial, plant, and animal cells as well as mushroom tissue. In order to measure the toxicity of tolaasin, erythrocytes of blood were used to evaluate the tolaasin-induced hemolysis. Hemolytic activity of tolaasin was measured by observing the absorbance change either at 420 nm, representing the release of hemoglobins from red blood cells(RBCs), or at 600 nm, representing the density of residual cells. The hemolytic activity of culture-extract of P. tolaasii increased at early-stationary phase of growth and was maximal at late stationary phase. The hemolytic activity of tolaasin appeared high in the RBCs of dog and rat. The RBCs of rabbit and hen were less susceptible to tolaasin. The effects of various cations were also measured. $Cd^{2+}$ and $La^{3+}$. as well as $Zn^{2+}$ appeared inhibitory to the tolaasin-induced hemolysis. The effects of various anions on tolaasin-induced hemolysis were measured and carbonate showed the greatest inhibition to the hemolysis. However, phosphate stimulated the tolaasin-induced hemolysis and no effects were observed by chloride and nitrate.

• The Korean Journal of Mycology
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• v.25 no.4 s.83
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• pp.253-256
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• 1997

To elucidate the hemolytic toxicities of Korean basidiomycetes, the cold-water extracts of 46 wild mushrooms were tested for hemolytic activity on mouse erythrocytes. Twelve species including Amanita esculenta, A. griseofarinosa, A. longistriata, A. melleiceps, A. phalloides, A. rubescence, A. spissacea, A. vaginata, Hebeloma crustuliniforme, Lepiota cygnea, Macrolepiota procera, and Oudemansiella platyphylla showed strong hemolytic activities. Of these, A. griseofarinosa and H. crustuliniforme showed no decrease in their hemolytic activity even after five minutes' boiling.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.101-101
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• 1993

사용한 CPZ, PPZ, TFZ 및 TRZ의 약물이 UVA조사에 의해 용혈독성이 크게 나타났으며 CPZ, PPZ, TRZ에 의한 광용혈정도는 ascorbic acid에 의해 유의성 있게 감소되었다. 또한 적혈구를 가하기 전 각 약물을 미리 조사시켜 생성된 물질에 의한 광용혈현상의 광독성생성물질은 chlorpromazine과 thioridazine에서 보여졌으며, cholrpromazine의 광독성생성물질에 의한 적혈구 용혈현상만 ascorbic acid에 의해 감소되었다. UVA조사전 후의 각 약물에 대한 TA98, TA100에서의 발암성은 인정되지 않았다.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.455-456
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• 2000

병원성 Vibrio spp등의 추정되는 병원성 인자들로서는 Hemolysin(용혈소) Protease, Phospholipase와 Siderophores등 여러 가지가 있으며 이 가운데 Hemolysin 은 공통인자로 중요시되어 왔다. V. fluvialis는 자연환경에서도 분리가능 하였지만, 임상에서도 분리되었다. 본 연구에서는 병원성 비브리오속인 V. fluvialis의 용혈소를 분리정제하여 그 물리화학적 특성을 확인하였다. (중략)

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.328-328
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• 1994

광독성 및 광알러지를 일으키는 제반 약물이나 화학물질의 안정성과생체 이용율을 높이고 현재까지 확실하게 규명되어있지 않은 약물 및 화학물질에의한 광독성 광알러지성의 기전을 이해하고 설명할수있는 학문적 기초를 확립 하고저 phenothiazines 중 몇가지 약물를 택하여 적혈구를 이용한 광용혈 여부와 이에 미치는 ascorbic acid, butyl hydroxy anisole, dibutyl hydroxy toluene의 영향을 조사하였다. 각 약물 농도를 50$\mu\textrm{g}$/m1로 하고 UVA(350nm.2.5 ㎽/cm)조사시간은 30분으로 하여 적혈구의 광용혈현상을 Kahn등의 방법에의해 spectrophotometer로 측정한결과 chlorpromazine, perphenazine, thioridazine매 의한 광용혈정도는 ascorbic acid에의해 유의성 있게 감소되었다. 또한 적혈구를 가하기 전 각 약물을 미리조사시켜 생성된 물질에의한 광용혈현상의 광독성생성물질은 chlor- promazine과 thioridazine에서 보여졌으며, chlorpromazine의 광독성생성물질에 의한 적혈구 용혈현상만 ascorbic acid에의해 감소되었다.

• Clinical and Experimental Pediatrics
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• v.50 no.12
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• pp.1261-1265
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• 2007

Extrarenal manifestations of hemolytic uremic syndrome (HUS) have increasingly been recognized and may be major determinants of mortality and morbidity. Although microthrombi are often found in the pulmonary and coronary circulation, apparent lung and cardiac involvement are clinically infrequent. We describe here a 10-month-old boy with HUS who developed pulmonary hemorrhage, acute respiratory distress syndrome and dilated cardiomyopathy. Complete renal as well as clinical recovery from these very uncommon complications was achieved by optimum supportive care.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1992.05a
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• pp.52-52
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• 1992

cisplatin 자체는 용혈작용이 고농도에서 얼었는데 반하여 호중구 자극상등액에서는 저농도에서 높은 용혈작용을 나타내 신장독성이 호중구 활성화에 기인할 것이라는 가능성을 시사하였다. In vitro에서 대부분의 활성산소 제거제는 세포독성 억제효과가 있었으며 in vivo 투여시에도 합성 항산화제인 BHA 등은 혈청 BUN, creatinine치의 현저한 감소를 나타내 좋은 신장독성억제 효과를 보여주었다.

• Korean Journal of Microbiology
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• v.34 no.1_2
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• pp.26-30
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• 1998

Tetradecapeptide, Mastoparan B(MP B) and its [$Ala^2$]-, [$Ala^4$]-, [$Ala^6$]-, [$Ala^9$]-MP B derivatives were synthesized, and then their antibacterial and hemolytic activities were assayed to examine the effect of hydrophobicity and amphiphilicity on the MP B-induced those activities. MP B and more hydrophobic [$Ala^2$]-, [$Ala^4$]-MP B showed stronger antibacterial activity and less hydrophobic [$Ala^6$]-MP B than MP B did similar or weaker activity, so more hydrophobic [Ala]-MP B derivative had stronger activity. But more hydrophobic [$Ala^9$]-MP B than MP B showed weaker activity because of its Trp substitution by Ala. On the other hand, [$Ala^2$]- and [$Ala^4$]-MP B showed 100.0% and 69.4% hemolytic activity, but [$Ala^6$]-MP B did the weakest activity(6.1%) and [$Ala^9$]-MP B, weaker activity(26.0%) than MP-B. Therefore, more hydrophobic [Ala]-MP B derivative had stronger activity and the effect of amphiphilicity on the activity was weak.