• Title/Summary/Keyword: 요소기반 분할

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Acoustic technology-assisted rapid proteolysis for high-throughput proteome analysis (대량 발굴 프로테옴 분석을 위한 어쿠스틱 기술 기반 고속 단백질 절편화)

  • Kim, Bo-Ra;Huyen, Trang Tran;Han, Na-Young;Park, Jong-Moon;Yu, Ung-Sik;Lee, Hoo-Keun
    • Analytical Science and Technology
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    • v.24 no.6
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    • pp.510-518
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    • 2011
  • Recent developments and improvements of multiple technological elements including mass spectrometry (MS) instrument, multi-dimensional chromatographic separation, and software tools processing MS data resulted in benefits of large scale proteomics analysis. However, its throughput is limited by the speed and reproducibility of the protein digestion process. In this study, we demonstrated a new method for rapid proteolytic digestion of proteins using acoustic technology. Tryptic digests of BSA prepared at various conditions by super acoustic for optimization time and intensity were analyzed by LC-MS/MS showed higher sequence coverage in compared with traditional 16 hrs digestion method. The method was applied successfully for complex proteins of a breast cancer cells at 30 min of digestion at intensity 2. This new application reduces time-consuming of sample preparation with better efficiency, even with large amount of proteins, and increases high-throughput process in sample preparation state.

A Basic Study on Prediction Module Development of Collision Risk based on Ship's Operator's Consciousness (선박운항자 의식 기반 충돌 위험도 예측 모듈 개발에 관한 연구)

  • Park, Young-Soo;Park, Sang-Won;Cho, Ik-Soon
    • Journal of Navigation and Port Research
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    • v.39 no.3
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    • pp.199-207
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    • 2015
  • In ports of Korea, the marine traffic flow is congested due to a large number of vessels coming in and going out. In order to improve the safety and efficiency of these vessels, South Korea is operating with a Vessel Traffic Service System, which is monitoring its waters for 24 hours. However despite these efforts of the VTS (Vessel Traffic Service) officers, collisions are occurring continuously, the risk situation is analyzed that occurs once in about 20 minutes, the risk may be greater. It investigated to reduce these accidents by providing a safety standard for collision danger in a timely manner. Thus, this study has developed a risk prediction module to predict risk in advance. This module can avoid collision risk to adjust the speed and course of ship using a risk evaluation model based on ship operator's risk perspective. Using this module, the ship operators and VTS officers can easily be identified risks in complex traffic situations, so they can take an appropriate action against danger in near future including course and speed change. To verify the effectiveness of this module, this paper predicted the risk of each encounter situation and confirmed to be capable of identifying a risk changes in specific course and speed changes at Busan coastal water.

Color Vision Based Close Leading Vehicle Tracking in Stop-and-Go Traffic Condition (저속주행환경에서 컬러비전 기반의 근거리 전방차량추적)

  • Rho, Kwang-Hyun;Han, Min-Hong
    • The Transactions of the Korea Information Processing Society
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    • v.7 no.9
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    • pp.3037-3047
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    • 2000
  • This paper describes a method of tracking a close leading vehicle by color image processing using the pairs of tail and brake lights. which emit red light and are housed on the rear of the vehicle in stop-and-go traffic condition. In the color image converted as an HSV color model. candidate regions of rear lights are identified using the color features of a pair of lights. Then. the pair of tailor brake lights are detected by means of the geometrical features and location features for the pattern of the tail and brake lights. The location of the leading vehicle can be estimated by the location of the detected lights and the vehicle can be tracked continuously. It is also possible to detect the braking status of the leading vehicle by measuring the change in HSV color components of the pair of lights detected. In the experiment. this method tracked a leading vehicle successfully from urban road images and was more useful at night than in the daylight. The KAV-Ill (Korea Autonomous Vehicle- Ill) equipped with a color vision system implementing this algorithm was able to follow a leading vehicle autonomously at speeds of up to 15km!h on a paved road at night. This method might be useful for developing an LSA (Low Speed Automation) system that can relieve driver's stress in the stop-and-go traffic conditions encountered on urban roads.

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A Study on the Life Cycle Analysis of Domestic Tourist Areas (국내 관광지의 수명주기 분석 연구)

  • Kim, Jung Jae;Lee, Kyung Jin
    • Journal of the Korean Institute of Landscape Architecture
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    • v.43 no.6
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    • pp.25-40
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    • 2015
  • Korean tourist areas are designated/managed in accordance with the Tourism Promotion Act. Since Taejongdae was designated as a tourist area in 1969, a total of 230 tourist areas have been designated/built/operated. It has been 46 years since the first tourist area was designated. According to the Tourism Area Life Cycle by Butler, it is estimated that the flow of the life cycle will be reflected in ups and downs over time. Thus, this study aimed to provide basic data for suggesting the direction of changes and development of the future tourist areas after analyzing the life cycle stage of domestic tourist areas, by applying the Tourism Area Life Cycle by Butler. The research method was based on the tourist areas by year, the number of visitors, and data of the target to derive the change transition curve, obtained by dividing the life cycle stages of the tourist areas based on the visitor rate of change. In the analysis results, more than 1/3 of domestic tourist areas are reaching the stagnation/decline stage, and tourist areas such as hot springs and seaside/beach resources show a particularly high ratio of stagnation/decline. The tourist areas that already have reached the stagnation/decline stage will need to analyze the causes for the decline, seek for resolution measures, and introduce new innovative elements. Even though the results of this study are not sufficient to be used as an absolute standard to decide the life cycle stage of domestic tourist areas, it is considered to be adequate for phenomenologically understanding the life cycle stage of Korean tourist areas. Based on this study, the causes for the stagnation/decline of tourist areas can be revealed while it can be also used as basic research to establish revitalization measures for tourist areas by introducing new innovation.

Skeleton Code Generation for Transforming an XML Document with DTD using Metadata Interface (메타데이터 인터페이스를 이용한 DTD 기반 XML 문서 변환기의 골격 원시 코드 생성)

  • Choe Gui-Ja;Nam Young-Kwang
    • The KIPS Transactions:PartD
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    • v.13D no.4 s.107
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    • pp.549-556
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    • 2006
  • In this paper, we propose a system for generating skeleton programs for directly transforming an XML document to another document, whose structure is defined in the target DTD with GUI environment. With the generated code, the users can easily update or insert their own codes into the program so that they can convert the document as the way that they want and can be connected with other classes or library files. Since most of the currently available code generation systems or methods for transforming XML documents use XSLT or XQuery, it is very difficult or impossible for users to manipulate the source code for further updates or refinements. As the generated code in this paper reveals the code along the XPaths of the target DTD, the result code is quite readable. The code generating procedure is simple; once the user maps the related elements represented as trees in the GUI interface, the source document is transformed into the target document and its corresponding Java source program is generated, where DTD is given or extracted from XML documents automatically by parsing it. The mapping is classified 1:1, 1:N, and N:1, according to the structure and semantics of elements of the DTD. The functions for changing the structure of elements designated by the user are amalgamated into the metadata interface. A real world example of transforming articles written in XML file into a bibliographical XML document is shown with the transformed result and its code.

Concepts and functional characteristics of consciousness in comparison of memory and attention (기억과 주의와의 비교를 통한 의식의 개념과 기능적 특성)

  • Kim, Eun-Sook;Shin, Hyun-Jung
    • Korean Journal of Cognitive Science
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    • v.21 no.4
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    • pp.559-602
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    • 2010
  • This study examines the concepts and functional characteristics of consciousness in comparison of memory and attention from the perspective of information processing. It also provides an understanding of the relationships between the three as hypothetical constructs. Consciousness is regarded as too ambiguous a concept to be understood and accepted as a mental construct without the inclusion of memory and attention in any conceptualization. We need one criterion to count satisfactorily as an explanation of consciousness in information processing. Consciousness would be a subjective awareness of momentary experience and also have the characteristic of an operating system performing control and consolidation in information processing. This could be called cognitive consciousness which refers to a subjective awareness and an executive control system, even though those are not equivalent concepts. Consciousness, memory and attention, three mental constructs could operate dependently or independently depending on the specific tasks conditioned in many information processing levels whose modules with three mental constructs could operate in hierarchy. In this premise, working memory could not be a unitary system, contrary to those of Baddeley and Hitch(1974) and Baddeley(1992, 2000), just being a mental workplace consisting of two components: the phonological loop, and the visual-spatial sketchpad without the episodic buffer and a central executive which are the characteristics of consciousness. In the continuum of information processing, the conscious processes and the unconscious processes seem not to be totally different and contrasting processes.

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A Tablet PC-Based Music-Making Program for Improving Executive Function of Adolescents With Intellectual Disabilities (지적장애 청소년의 집행기능 향상을 위한 태블릿 PC 기반 음악 만들기 활동)

  • Ji, Kyeongmi
    • Journal of Music and Human Behavior
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    • v.12 no.1
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    • pp.1-21
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    • 2015
  • This study examined the effects of a tablet PC-based music-making program on the executive function of adolescents with intellectual disabilities. Four adolescents with intellectual disabilities participated in this program. Each participant received 45-minute individual sessions twice a week for a total of 16 sessions. The music-making program was designed in the sequence of planning; learning table PC operations; exploring musical elements; making rhythm, melody, and lyrics; composing loop sections; and presentation of the completed music. The Stroop test, Children's Color Trails Test, and Digit Span and Letter-Number sequencing tests were measured at pretest, midtest, and posttest in order to examine changes in executive function. The participants showed increased scores on all three tests. The participants' attention span also increased and their attempts to correct errors during tasks occurred more frequently at posttest. This study supports the effects of the technology-based program on the executive function of adolescents with intellectual disabilities and presents its expanded applicability for adolescents who show low cognitive function and limited motivation for cognitive engagement.

growth of Cadmium Sulfide (CdS) Thin Film by Solution Growth Technique and Study of Quantum Size Effects (용액성장법에 의한 Cadmium Sulfide(CdS) 박막 성장 및 양자 사이즈 효과에 관한 연구)

  • 임상철
    • Journal of the Microelectronics and Packaging Society
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    • v.4 no.1
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    • pp.1-12
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    • 1997
  • 본 연구에서는 용액성장법에 의해 양자 입자로 구성된 CdS 박막을 슬라이드 유리기 판위에 성장시키고 이들의 구조적 광학적 특성에 대하여 연구하였고 이들 결과를 토대로 용 액성장법으로 성장된 CdS 박막의 양자 사이즈 효과에 대하여 연구하였다. 성장시간은 1, 3, 10, 20분이었고 성장온도는 75$^{\circ}C$였다. X-선 회절 분석결과 본 연구에서 합성된 CdS 박막은 hexagonal상의 결정구조를 갖는 것으로 나타났고 성장시간에 따라 막의 투께는 61~195nm, 입자사이즈는 8.5~22.5nm로 나타났다. 광에너지 변화에 따른 투과도 측정결과 본 연구의 CdS 시료는 성장시간에 따라 에너지 밴드갭이 2.43~2.51 eV로 나타나서 벌크 CdS의 에너 지 밴드갭인 2.42 ev보다 높은 에너지 밴드갭을 갖게 되어 양자 사이즈 효과에 의한 blue shift 현상이 용액성장법에 의해 합성된 CdS 시료에도 존재한다는 것이 밝혀졌다 그리고 이 같은 용액성장법으로 성장된 CdS에 대해 최초로 수행된 Raman 산란 실험결과 이성장방법 으로 성장된 CdS에는 1TO, E2, 1LO 포논 모드가 존재함을 알수 있었고 또한 입자 사이즈 감소에 의한 1LO포논 모드의저주파수 shift 현상 즉 포논 모드의 softening 현상이 있음이 밝혀졌고 softening은 최대6.2%까지 발생하였다. 이와같은 높은 softening은 본연구의 CdS 박막 내 양자 입자의 입도가 작은것에 기인하는 것으로 밝혀졌다. 또한 본 CdS 시료의 양 자 사이즈 효과의 결과로 1TO 포논도 나타났는데 이 1TO 포논과 E2 포논의 Raman shift 는 성장시간 즉 막의 두께와는 무관한 것으로 나타났다.행렬모형(二重比例行列模型)을 이용하여, 산업구조의 변화로 인한 직업별 인력수요 변화가 충분히 고려되도록 하였다. 전망의 결과에 따르면 향후 우리 경제는 지식기반경제(knowledge-based economy)로 이행하고 있다고 볼 수 있다. 우선 산업구조면에서 지식집약적산업으로의 구조조정이 일어나게 되고 이에 따라 산업별 취업구조에서도 고기술산업의 취업준비중이 급속히 증가하게 된다. 직업별 취업분포에 있어서도 전문기술직 행정관리직 등의 고숙련 사무직의 비중은 크게 증가하는 반면 생산관련직과 농림어업직의 비중은 감소하게 된다. 이처럼 경제가 지식집약화되어 감에 따라 고학력자에 대한 수요는 지속적으로 증가하지만 현재 적절한 인력양성과 공급이 이루어지지 않고 있어 향후 기술이나 기능에 따른 수급부일정(需給不一政)(skill mismatch)현상이 매우 심해질 것으로 보인다. 따라서 앞으로의 인력정책에서 가장 주안점을 두어야 할 부분은 첨단기술산업과 관련된 인력의 양성에 있다고 하겠다.2시간까지 LPDG용액은 MEC용액보다 비교적 나은 회복을 보였고 재관류 3일과 7일의 폐기능 평가에서 두 용액 모두에서 폐기능의 점차적 소실을 보였으며 이는 병리조직검사에서 보듯이 폐혐에 의한 외적인 요소라고 생각되며 따라서 LPDG용액은 허혈재관류손상 방지 및 급성폐렴 등 염증을 잘 관리한다면 20시간 이상 LPDG용액의 안전한 폐보존의 가능성 을 얻을 수 있었다.ic 형태로 외래유전자가 발현되었지만 대조구에서 87.0% (26/30개) 배반포기가 $\beta$-Gal 활력을 보인 반면, G418 처리구에서는 모든 배반포기가 $\beta$-Gal 활력을 보였다 (P<0.05). 그러나 대조구 및 G418 처리구의 ICM

Water Balance of a Small Catchment in the Subalpine Grassland of Mt. Halla, Southern Korea (한라산 아고산 초지대 소유역의 물수지)

  • An Jung-Gi;Kim Tae-Ho
    • Journal of the Korean Geographical Society
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    • v.41 no.4 s.115
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    • pp.404-417
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    • 2006
  • In order to clarify some characteristics of hydrological cycle in the subalpine zone of Mt. Halla, water balance has been analysed using hydrological data of a first-order drainage basin around Mansedongsan and meterological data of Odeung AWS. The experimental basin extends from 1,595 m to 1,645 m in altitude and has an catchment area of 1.34 ha. It is largely underlain by trachybasalt and covered with sasa bamboo and sedges. Hydrological observations were carried out every 20 minutes from April 15 to September 19, 2004. The basin shows the total precipitation of 3,074 m that is 1.6 to 3 times of those in coastal and intermontane regions. Surface runoff amounts to 850 mm that is equivalent to 27.6% of the precipitation. By contrast, evapotranspiration only accounts for 14.2% of the precipitation, and the remnant of 1,790 m penetrates underground through a basement. The basin is located in the subalpine zone and then it has a high rainfall intensity as well as a large rainfall due to frequent orographic precipitation. But surface runoff usually dose not exceed 30% of the rainfall while Percolation demonstrates about 2 times of the runoff. Compared with granite or gneiss basins in Korea Peninsula, the experimental basin is characterized by a higher portion of percolation in water balance. And it is probably related to the highly permeable basaltic lavas in Jeju Island which are also overlain by porous volcanic soils.

Defining the Tumour and Gross Tumor Volume using PET/CT : Simulation using Moving Phantom (양전자단층촬영장치에서 호흡의 영향에 따른 종양의 변화 분석)

  • Jin, Gye-Hwan
    • Journal of the Korean Society of Radiology
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    • v.15 no.7
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    • pp.935-942
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    • 2021
  • Involuntary movement of internal organs by respiration is a factor that greatly affects the results of radiotherapy and diagnosis. In this study, a moving phantom was fabricated to simulate the movement of an organ or a tumor according to respiration, and 18F-FDG PET/CT scan images were acquired under various respiratory simulating conditions to analyze the movement range of the tumor movement by respiration, the level of artifacts according to the size of the tumor and the maximum standardized uptake value (SUVmax). Based on Windows CE 6.0 as the operating system, using electric actuator, electric actuator positioning driver, and programmable logic controller (PLC), the position and speed control module was operated normally at a moving distance of 0-5 cm and 10, 15, and 20 reciprocations. For sphere diameters of 10, 13, 17, 22, 28, and 37 mm at a delay time of 100 minutes, 80.4%, 99.5%, 107.9%, 113.1%, 128.0%, and 124.8%, respectively were measured. When the moving distance was the same, the difference according to the respiratory rate was insignificant. When the number of breaths is 20 and the moving distance is 1 cm, 2 cm, 3 cm, and 5 cm, as the moving distance increased at the sphere diameters of 10, 13, 17, 22, 28, and 37 mm, the ability to distinguish images from smaller spheres deteriorated. When the moving distance is 5 cm compared to the still image, the maximum values of the standard intake coefficient were 18.0%, 23.7%, 29.3%, 38.4%, 49.0%, and 67.4% for sphere diameters of 10, 13, 17, 22, 28, and 37 mm, respectively.